CD44 Antibody

Images

 
Western Blot: CD44 Antibody [NBP1-84578] - Analysis in human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: CD44 Antibody [NBP1-84578] - Staining of human cell line U-251 MG shows localization to plasma membrane.
Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Immunohistochemical staining of human skin shows strong membranous positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Immunohistochemical staining of human breast shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Immunohistochemical staining of human squamous cell lung carcinoma shows strong membranous positivity in tumor cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CD44 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:DDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIPATTLMSTSATATETATKRQETWDWFSWLFLPSESKNHLHTTTQMAGTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPR
Marker
Cell Membrane Marker
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD44
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CD44 Protein (NBP1-84578PEP)
Publications
Read Publications using
NBP1-84578 in the following applications:

Reactivity Notes

Reactivity reported in scientific literature (PMID: 18241271)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CD44 Antibody

  • CD44 antigen
  • CD44 molecule (Indian blood group)
  • CD44
  • CD44R
  • CDw44
  • cell surface glycoprotein CD44
  • chondroitin sulfate proteoglycan 8
  • CSPG8
  • ECMR-III
  • epican
  • Extracellular matrix receptor III
  • GP90 lymphocyte homing/adhesion receptor
  • HCAM
  • HCELL
  • hematopoietic cell E- and L-selectin ligand
  • Heparan sulfate proteoglycan
  • Hermes antigen
  • homing function and Indian blood group system
  • HUTCH-I
  • Hyaluronate receptor
  • IN
  • LHR
  • MC56
  • MDU2
  • MDU2CD44 antigen (homing function and Indian blood group system)
  • MDU3
  • MDU3CDW44
  • MIC4
  • MIC4MGC10468
  • MUTCH-I
  • Pgp1
  • PGP-1
  • PGP-I
  • Phagocytic glycoprotein 1
  • Phagocytic glycoprotein I

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD44 Antibody (NBP1-84578)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(2)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-84578 Applications Species
Helen Schneck, Berthold Gierke, Frauke Uppenkamp et al. EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer. PLOS ONE 2015 Dec 22 [PMID: 26859839] (ICC/IF, Human) ICC/IF Human
Ran Ma, Irma Fredriksson, Govindasamy-Muralidharan Karthik et al. Superficial scrapings from breast tumors is a source for biobanking and research purposes. Laboratory Investigation 2014 [PMID: 24776644] (ICC/IF, Human) ICC/IF Human
Baccelli I, Schneeweiss A, Riethdorf S et al. Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay. Nat Biotechnol 2013 Jun [PMID: 23609047]
Petit V, Massonnet G, Maciorowski Z et al. Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters. Lab Invest 2013 May [PMID: 23459372]
Vazquez-Martin A, Oliveras-Ferraros C, Cufi S et al. Metformin regulates breast cancer stem cell ontogeny by transcriptional regulation of the epithelial-mesenchymal transition (EMT) status. Cell Cycle 2010 Sep 15 [PMID: 20890129]
Twarock S, Rock K, Sarbia M et al. Synthesis of hyaluronan in oesophageal cancer cells is uncoupled from the prostaglandin-cAMP pathway. Br J Pharmacol 2009 May [PMID: 19338584]
Asplund A, Gry Bjorklund M, Sundquist C et al. Expression profiling of microdissected cell populations selected from basal cells in normal epidermis and basal cell carcinoma. Br J Dermatol 2008 Mar [PMID: 18241271]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD44 Antibody (NBP1-84578) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD44
Entrez