Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A7L6)

Immunohistochemistry-Paraffin: Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A7L6) [NBP2-44448] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Heparan Sulfate Monoclonal Antibody (A7L6).

• Reactivity: Hu, Mu, Po, Bv, Fi, Pm
• Applications: Flow, ICC/IF, IHC, ICC/IF
• Clone: A7L6
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A7L6) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-47695)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Murine EHS laminin preparation
• Localization: Basement membrane
• Specificity: This monoclonal antibody specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecans strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: HSPG2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined. Use in IHC-Fr reported in scientific literature (PMID:34228647).
• Theoretical MW: 400 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Human reported in scientific literature (PMID:34228647)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A7L6)

• basement membrane-specific heparan sulfate proteoglycan core protein
• endorepellin (domain V region)
• heparan sulfate proteoglycan 2
• Heparan Sulfate Proteoglycan
• HSPG
• perlecan proteoglycan
• perlecan
• PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia)
• PRCAN
• SJA
• SJS
• SJS1

Background

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodeling (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (NBP2-44448)(1)

Publication using NBP2-44448

• Xie Y, He L, Lugano R Et Al. Key molecular alterations in endothelial cells in human glioblastoma uncovered through single-cell RNA sequencing JCI insight 2021-07-06 [PMID: 34228647] (IHC-P, IHC-Fr)

Bioinformatics

• Gene Symbol: HSPG2
• Uniprot:
  • P98160()
  • P98160()
  • P98160()