CD24 Antibody (SN3)

Flow Cytometry: CD24 Antibody (SN3) [NB100-64861] - Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD24: RPE.

• Reactivity: Hu
• Applications: Flow, IHC, IHC-Fr, IHC-P
• Clone: SN3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CD24 Antibody (SN3) Summary

• Immunogen: Glycoproteins purified from human NALM-1 cell line
• Specificity: Recognizes the human CD24 cell surface antigen, a heavily glycosylated molecule of 35-45kD. CD24 is expressed by granulocytes and B lymphocytes, and by some activated T cells and T cell malignancies.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD24
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1/50-1/100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
• Application Notes: This CD24 antibody is validated for IHC-P from a verified customer review.

Reactivity Notes

May react with Chimpanzee

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CD24 Antibody (SN3)

• CD 24
• CD24 antigen (small cell lung carcinoma cluster 4 antigen)
• CD24 antigen
• CD24 molecule
• CD24
• CD24A
• CD24Asignal transducer CD24
• FLJ22950
• FLJ43543
• MGC75043
• Small cell lung carcinoma cluster 4 antigen

Background

CD24 (also known as heat stable antigen) is a phosphatidylinositol linked cell surface glycoprotein with a polypeptide core of 30 amino acids and high carbohydrate content. The apparent molecular mass of the two forms of CD24 purified from a mouse lymphoma cell line appear to be in the range of 40-60 and 23-30 kDa. CD24 is expressed at all stages of B cell development and on most thymocytes. The absence of expression from mature T cells is closely associated with their maturation from CD4+ CD8+ CD24+ thymocytes to either CD4+ CD8- CD24- or CD4- CD8+ CD24- T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes. Its ligand is P selectin (CD62P). CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes. CD24 is also a positive marker of pancreatic cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD24 Antibody (NB100-64861)(2)

Publications using NB100-64861

• Takashima K, Fujii S, Komatsuzaki R et al. CD24 and CK4 are upregulated by SIM2, and are predictive biomarkers for chemoradiotherapy and surgery in esophageal cancer Int J Oncol Jan 17 2020 [PMID: 32124945]
• Barkal AA, Brewer RE, Markovic M et al. CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy Nature Aug 1 2019 [PMID: 31367043] (Flow, Human)

Review for CD24 Antibody (NB100-64861) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Human 03/25/2020

Summary

• Sample Tested: Gastric carcinoma
• Species: Human
• Lot: N/A

Comments

• Comments: pH 9 antigen retrieval

Bioinformatics

• Gene Symbol: CD24