Syndecan-2/CD362 Antibody (305515) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Rat Anti-Human Syndecan-2/CD362 Monoclonal Antibody (Catalog # MAB2965) or (B) Rat IgG2BIsotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 305515
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Syndecan-2/CD362 Antibody (305515) [Unconjugated] Summary

• Additional Information: Clone 305515 was used by HLDA to establish CD designation
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Syndecan-2/CD362
  Glu19-Gly144
  Accession # AAH49836.1
• Specificity: Detects human Syndecan-2/CD362 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Syndecan-1, recombinant human (rh) Syndecan-3, or rhSyndecan-4 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: SDC2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Syndecan-2/CD362 Antibody (305515) [Unconjugated]

• CD362 antigen
• CD362
• Fibroglycan
• heparan sulfate proteoglycan 1, cell surface-associated
• Heparan sulfate proteoglycan core protein
• HSPG
• HSPG1
• HSPG1syndecan proteoglycan 2
• SDC2
• SYND2
• SYND2cell surface-associated heparan sulfate proteoglycan 1
• syndecan 2
• Syndecan2
• Syndecan-2

Background

Syndecan-2, previously known as fibroglycan or heparan sulfate proteoglycan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans show conserved cytoplasmic domains and divergent extracellular portions (except for GAG attachment sites). Among the Syndecans, Syndecan-2 is most similar to Syndecan-4 (1‑3). Human Syndecan-2 is synthesized as a 201 amino acid (aa) core protein with an 18 aa signal sequence, a 126 aa extracellular domain (ECD), a 25 aa transmembrane region and a 32 aa cytoplasmic tail (4). The human ECD of Syndecan-2 contains three closely-spaced consensus Ser-Gly sequences for the attachment of HS side chains. It shares 76%, 73%, 87%, 78% and 63% aa identity with the ECD of mouse, rat, bovine, canine and chicken Syndecan-2, respectively. The cytoplasmic tail has both serine and tyrosine phosphorylation sites. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 22 kDa core protein. Non-covalent homodimerization of Syndecan-2 is dependent on the transmembrane domain (5). Syndecan-2 is expressed in cells of mesenchymal origin, neuronal and epithelial cells, and is the predominant syndecan expressed during embryonic development. Expression is upregulated in several cancer cell lines (6). After induction in macrophages by inflammatory mediators, Syndecan-2 selectively binds FGFbasic, VEGF and EGF (7). Syndecan-2 expressed on human primary osteoblasts binds GM-CSF and may function as a co‑receptor (8). Activated endothelial cell Syndecan-2 specifically binds IL-8 and may participate in promoting neutrophil extravasation by forming a chemotactic IL-8 gradient (9). Typically, cytokine, chemokine and extracellular matrix protein binding occurs through interaction with HS side chains, but the Syndecan-2 extracellular domain can bind TGF-beta directly via protein-protein interaction (10).

1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
2. Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
3. Essner, J. J. et al. (2006) Int. J. Biochem. Cell Biol. 38:152.
4. Marynen, P. et al. (1989) J. Biol. Chem. 264:7017.
5. Choi, S. et al. (2005) J. Biol. Chem. 280:42573.
6. Park, H. et al. (2002) J. Biol. Chem. 277:29730.
7. Clasper, S. et al. (1999) J. Biol. Chem. 274:24113.
8. Modrowski, D. et al. (2000) J. Biol. Chem. 275:9178.
9. Halden, Y. et al. (2004) Biochem. J. 377:533.
10. Chen, L. et al. (2004) J. Biol. Chem. 279:15715.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Syndecan-2/CD362 Antibody (MAB2965)(6)

Publications using MAB2965

• Hsieh, LT;Hall, BS;Newcombe, J;Mendum, TA;Varela, SS;Umrania, Y;Deery, MJ;Shi, WQ;Diaz-Delgado, J;Salguero, FJ;Simmonds, RE; The Mycobacterium ulcerans toxin mycolactone causes destructive Sec61-dependent loss of the endothelial glycocalyx and vessel basement membrane to drive skin necrosis eLife 2025-02-06 [PMID: 39913180] (Flow Cytometry, Human)
• Matsunuma, M;Kan, R;Yamada, Y;Hamada, K;Kanagawa, M;Nomizu, M;Kikkawa, Y; Chain-specificity of laminin ?1-5 LG45 modules in the recognition of carbohydrate-linked receptors and intramolecular binding Scientific reports 2023-06-27 [PMID: 37369727] (Flow Cytometry, Human)
• LT Hsieh, BS Hall, J Newcombe, TA Mendum, Y Umrania, MJ Deery, WQ Shi, FJ Salguero, RE Simmonds Mycolactone causes catastrophic Sec61-dependent loss of the endothelial glycocalyx and basement membrane: a new indirect mechanism driving tissue necrosis in Mycobacterium ulcerans infection bioRxiv : the preprint server for biology, 2023-02-21;0(0):. 2/21/2023 [PMID: 36865118]
• DM Beauvais, SE Nelson, KM Adams, NA Stueven, O Jung, AC Rapraeger Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression The Journal of Biological Chemistry, 2022-05-13;0(0):102029. 2022-05-13 [PMID: 35569509] (Flow Cytometry, Human)
• Cabezon R, Sintes J, Llinas L, Benitez-Ribas D Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Immunol. Lett., 2010-10-07;134(2):167-73. 2010-10-07 [PMID: 20933012] (Flow Cytometry, Human)
• Matesanz-Isabel J, Sintes J, Llinas L, de Salort J, Lazaro A, Engel P New B-cell CD molecules. Immunol. Lett., 2010-10-07;134(2):104-12. 2010-10-07 [PMID: 20933010] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: SDC2
• Uniprot:
  • AAH49836.1()