Integrin beta 1/CD29 Antibody (P5D2) [Unconjugated]

Images

 
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open ...read more
Integrin beta 1/CD29 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781) at 25 µg/mL for 3 hours at ...read more
A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), ...read more
Integrin beta 1/CD29 knockout A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17781, filled histogram) or isotype control antibody (Catalog ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IP, B/N, CyTOF-ready, ICC/IF, KO
Clone
P5D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Integrin beta 1/CD29 Antibody (P5D2) [Unconjugated] Summary

Immunogen
Human skin keratinocytes
Specificity
Detects human Integrin beta 1/CD29.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGB1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunoprecipitation
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Knockout Validated
Control
Integrin beta 1/CD29 Knockout HeLa Cell Lysate
Publications
Read Publications using
MAB17781 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 1/CD29 Antibody (P5D2) [Unconjugated]

  • CD29 antigen
  • CD29
  • Fibronectin receptor subunit beta
  • FNRBVLAB
  • GPIIA
  • Integrin beta 1
  • integrin beta-1
  • integrin VLA-4 beta subunit
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
  • ITGB1
  • MDF2
  • MSK12
  • very late activation protein, beta polypeptide
  • VLA-4 subunit beta
  • VLA-BETA

Background

The Integrin beta 1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits. It regulates not only its multiple ligands, but activates a signaling cascade in its expressing cells. CD29 is proposed to play a role in cell adhesion, apoptosis, and differentiation (1,2). Over amino acids (aa) 21-728, human and mouse share 92% aa sequence identity.
  1. Barkan, D. and A.F. Chambers (2011) Clin. Cancer Res. 17:7219.
  2. Humphries, M.J. (2000) Biochem. Soc. Trans. 28:311.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin beta 1/CD29 Antibody (MAB17781)(17)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Blocking, Flow Cytometry, ICC, IHC, Neutralization.


Filter By Application
Blocking
(1)
Flow Cytometry
(3)
ICC
(2)
IHC
(1)
Neutralization
(9)
All Applications
Filter By Species
Human
(15)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using MAB17781 Applications Species
A Rajan, BD Persson, L Frängsmyr, A Olofsson, L Sandblad, J Heino, Y Takada, AP Mould, LM Schnapp, J Gall, N Arnberg Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells Sci Rep, 2018;8(1):10019. 2018 [PMID: 29968781] (Flow Cytometry) Flow Cytometry
SF Ahmad, JK Brown, LL Campbell, M Koscielnia, C Oliver, N Wheelhouse, G Entrican, S McFee, GS Wills, MO McClure, PJ Horner, S Gaikoumelo, KF Lee, HOD Critchley, WC Duncan, AW Horne Pelvic Chlamydial Infection Predisposes to Ectopic Pregnancy by Upregulating Integrin ?1 to Promote Embryo-tubal Attachment EBioMedicine, 2018;0(0):. 2018 [PMID: 29500127] (Neutralization, Human) Neutralization Human
CJ Lovitt, TB Shelper, VM Avery Doxorubicin resistance in breast cancer cells is mediated by extracellular matrix proteins BMC Cancer, 2018;18(1):41. 2018 [PMID: 29304770] (Neutralization, Human) Neutralization Human
S Gopal, L Veracini, D Grall, C Butori, S Schaub, S Audebert, L Camoin, E Baudelet, A Radwanska, S Beghelli-d, SM Violette, PH Weinreb, S Rekima, M Ilie, A Sudaka, P Hofman, E Van Obberg Fibronectin-guided migration of carcinoma collectives Nat Commun, 2017;8(0):14105. 2017 [PMID: 28102238] (Neutralization, Human) Neutralization Human
Rickard J Storm Human adenovirus type 37 uses ?V?1 and ?3?1 integrins for infection of human corneal cells J. Virol, 2016;0(0):. 2016 [PMID: 27974569] (Flow Cytometry, Human) Flow Cytometry Human
R Bass Identification of novel peptide motifs in the serpin maspin that affect vascular smooth muscle cell function Biochim. Biophys. Acta, 2016;1864(2):336-344. 2016 [PMID: 27888098] (Neutralization, Human) Neutralization Human
CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes Sci Rep, 2016;6(0):34310. 2016 [PMID: 27670158] (Neutralization, Human) Neutralization Human
Zhang, Qian, Dove, Christop, Hor, Jyh Lian, Murdock, Heardley, Strauss-Albee, Dara M, Garcia, Jordan A, Mandl, Judith N, Grodick, Rachael, Jing, Huie, Chandler-Brown, Devon B, Lenardo, Timothy, Crawford, Greg, Matthews, Helen F, Freeman, Alexandr, Cornall, Richard, Germain, Ronald N, Mueller, Scott N, Su, Helen C DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity. J Exp Med, 2014;211(13):2549-66. 2014 [PMID: 25422492] (Blocking, Human) Blocking Human
Rui X, Mehrbod M, Van Agthoven J, Anand S, Xiong J, Mofrad M, Arnaout M The alpha-subunit regulates stability of the metal ion at the ligand-associated metal ion-binding site in beta3 integrins. J Biol Chem, 2014;289(33):23256-63. 2014 [PMID: 24975416] (Neutralization, Human) Neutralization Human
Dotterweich J, Ebert R, Kraus S, Tower R, Jakob F, Schutze N Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells. Cell Commun Signal, 2014;12(0):36. 2014 [PMID: 24965524] (Neutralization, Human) Neutralization Human
Show All 17 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Integrin beta 1/CD29 Antibody (MAB17781). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
    • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB17781U
Alexa Fluor 405 FAB17781V
Alexa Fluor 488 FAB17781G
Alexa Fluor 594 FAB17781T
Alexa Fluor 647 FAB17781R
Alexa Fluor 700 FAB17781N
Alexa Fluor 750 FAB17781S
Phycoerythrin FAB17781P

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Blogs on Integrin beta 1/CD29.

Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions
Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits....  Read full blog post.

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Bioinformatics

Gene Symbol ITGB1
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