CD44 Antibody (8E2F3)

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).

Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of FFPE human breast carcinoma tissues using CD44 antibody (8E2F3). The signal was developed using DAB based detection and the sections were processed for counterstaining with hematoxylin. The antibody generated mainly a membrane staining representative of CD44 protein.

Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left).

Western Blot: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis using anti-CD44 mAb against HeLa (1) and HUVE-12 (2) cell lysates.

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD44 Antibody [8E2F3] conjugated to DyLight 550 (NBP1-47386R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Western Blot: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis using anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).

Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of methanol-fixed A431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of PANC-1 cells using anti-CD44 mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). DRAQ5 fluorescent DNA dye (blue).

Flow (Intracellular): CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

• Reactivity: Hu, Mu, Rb(-), Pm, Ca, Eq
• Applications: WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-P
• Clone: 8E2F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CD44 Antibody (8E2F3) Summary

• Immunogen: Purified recombinant fragment of human CD44 (628-699) expressed in E. coli. [Uniprot: P16070]
• Localization: Membrane; Single-pass type I membrane protein.
• Marker: Cell Membrane Marker
• Predicted Species: Baboon (94%), Equine (93%), Canine (93%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD44
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow (Intracellular)
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Not reactive to rabbit per customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for CD44 Antibody (8E2F3)

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• Epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44, also known as IN, LHRMIC4, CDW44, HCELL, is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD44 Antibody (NBP1-47386)(16)

Publications using NBP1-47386

• Li H, Chaitankar V, Zhu J et al. Olfactomedin 4 mediation of prostate stem/progenitor-like cell proliferation and differentiation via MYC Scientific reports Dec 14 2020 [PMID: 33318499] (IHC-P, ICC/IF, Human)
• Chen PY, Qin L, Li G et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms Cell Stem Cell Apr 2 2020 [PMID: 32243809] (Mouse)
• Tsai FJ, Lai MT, Cheng J et al. Novel K6-K14 keratin fusion enhances cancer stemness and aggressiveness in oral squamous cell carcinoma Oncogene Mar 13 2019 [PMID: 30867567] (IHC, Mouse)
• Scimeca M, Urbano N, Bonfiglio R et al. Breast osteoblast-like cells: a new biomarker for the management of breast cancer. Br. J. Cancer. Oct 17 2018 [PMID: 30327566] (IHC-P, Human)
• Hassanzadeh H, Matin MM, Naderi-Meshkin H et al. Using paracrine effects of Ad-MSCs on keratinocyte cultivation and fabrication of epidermal sheets for improving clinical applications Cell Tissue Bank Aug 13 2018 [PMID: 30105667] (Flow, Human)
• Baek M, kim M, Lim JS et al. Epidermal-specific deletion of TC-PTP promotes UVB-induced epidermal cell survival through the regulation of Flk-1/JNk signaling. Cell Death Dis. Jun 28 2018 [PMID: 29955047] (WB, Mouse)
• Jeong YJ, Oh HK, Park SH, Bong JG. Association between inflammation and cancer stem cell phenotype in breast cancer. Nat Commun. [PMID: 29434947] (IHC-P, Human)
• Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018 Jan 24 [PMID: 29367423] (Flow, Mouse)
  
  Details:
  This citation used the Allophycocyanin form of this antbody
• Lee HC, Ling QD, Yu WC et al. Drug-resistant colon cancer cells produce high carcinoembryonic antigen and might not be cancer-initiating cells. Drug Des Devel Ther. 2013 Jun 17 [PMID: 23818760] (IHC, Mouse)
• Chefetz I, Alvero AB, Holmberg JC et al. TLR2 enhances ovarian cancer stem cell self-renewal and promotes tumor repair and recurrence. Cell Cycle 2013 Feb 1 [PMID: 23324344] (IHC-P, FLOW, WB, Human)
• Papakonstantinou E, Klagas I, Karakiulakis G et al. Steroids and beta2-Agonists Regulate Hyaluronan Metabolism in Asthmatic Airway Smooth Muscle Cells Am J Respir Cell Mol Biol 2012 Dec [PMID: 22865625]
• Du W, Wright BM, Li X, Finke J, Rini BI, Zhou M, He H, Lal P, Welford SM. Tumor-derived macrophage migration inhibitory factor promotes an autocrine loop that enhances renal cell carcinoma. Oncogene. 2012 Apr 30. [PMID: 22543583] (WB, Human)
• Marzban H, Sasani F Canine Mammary Gland Cancer Stem Cell and its Potential Role in Malignant Biologic Behavior Iranian Journal of Veterinary Medicine Jan 1 2020 (WB, Canine)
• Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (Flow, Mouse)
  
  Details:
  Citation using the Allophycocyanin format of this antibody.
• Saxena N, Bhardwaj G, Jadhav S, et al. Pre-metastatic niche drives breast cancer invasion by modulating MSC homing and CAF differentiation bioRxiv Jan 15 2021 (ICC/IF, Human)
• Byun Js, Oh M, Lee S Et Al. The transcription factor PITX1 drives astrocyte differentiation by regulating the SOX9 gene J. Biol. Chem. Aug 5 2020 [PMID: 32759168] (IHC, Rat)

Review for CD44 Antibody (NBP1-47386) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Corey . WB Human 07/12/2013

Summary

• Application: Western Blot
• Sample Tested: Human Brain Tissue
• Species: Human
• Lot: 130226
• Comments: Working in human tissue with non-specific signal at 50 kDa

Blocking

• Blocking Details: 5% Milk, 2 hours at RT

Primary Anitbody

• Dilution Ratio: 1:1000, TBST over night in cold room

Secondary Antibody

• Secondary Description: Goat anti Mouse/ HRP
• Secondary Manufacturer Cat#: Jackson Immuno
• Secondary Concentration: 1:10000

Details

• Detection Notes: ECL-Plus

Comments

• Comments: Working in human tissue with non-specific signal at 50 kDa

Bioinformatics

• Gene Symbol: CD44
• Entrez:
  • 960(Human)
• Uniprot:
  • P16070()