CD44 Antibody (8E2F3)

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Confocal analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human breast carcinoma tissues using CD44 antibody (clone 8E2F3). The signal was developed using DAB based detection and the sections were processed for counterstaining with hematoxylin. The antibody generated mainly a membrane staining representative of CD44 protein.

Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Flow cytometric analysis of Hela cells using anti-CD44 mAb (right) and negative control (left).

Western Blot: CD44 Antibody (8E2F3) [NBP1-47386] - Western blot analysis using anti-CD44 mAb against Hela (1) and HUVE-12 (2) cell lysates.

Western Blot: CD44 Antibody (8E2F3) [NBP1-47386] - Western blot analysis using anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).

Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with CD44 Antibody (8E2F3) NBP1-47386AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Confocal immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Confocal immunofluorescence analysis of PANC-1 cells using anti-CD44 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Flow (Intracellular): CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with CD44 Antibody (8E2F3) NBP1-47386AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

• Reactivity: Hu, Mu, Rb(-), Bb, Ca, Eq
• Applications: WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-P
• Clone: 8E2F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CD44 Antibody (8E2F3) Summary

• Immunogen: Purified recombinant fragment of human CD44 (628-699) expressed in E. coli. [UniProt# P16070]
• Localization: Membrane; Single-pass type I membrane protein.
• Marker: Cell Membrane Marker
• Predicted Species: Baboon (94%), Equine (93%), Canine (93%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD44
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Flow (Intracellular)
• Application Notes: This CD44 (8E2F3) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Positive Control:

  HeLa Lysate (NB800-PC1)
  CD44 Lysate (NBP2-64923)

Reactivity Notes

Not reactive to rabbit per customer review. Immunogen's sequence homology with other species: Bovine (89%), Rat (87%), Golden hamster (87%) and Chinese hamster (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for CD44 Antibody (8E2F3)

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44, also known as IN, LHRMIC4, CDW44, HCELL, is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD44 Antibody (NBP1-47386)(5)

Publications using NBP1-47386

• Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018 Jan 24 [PMID: 29367423] (Flow, Mouse)
  
  Details:
  This citation used the Allophycocyanin form of this antbody
• Lee HC, Ling QD, Yu WC et al. Drug-resistant colon cancer cells produce high carcinoembryonic antigen and might not be cancer-initiating cells. Drug Des Devel Ther. 2013 Jun 17 [PMID: 23818760] (IHC, Mouse)
• Chefetz I, Alvero AB, Holmberg JC et al. TLR2 enhances ovarian cancer stem cell self-renewal and promotes tumor repair and recurrence. Cell Cycle 2013 Feb 1 [PMID: 23324344] (IHC-P, FLOW, WB, Human)
• Papakonstantinou E, Klagas I, Karakiulakis G et al. Steroids and beta2-Agonists Regulate Hyaluronan Metabolism in Asthmatic Airway Smooth Muscle Cells Am J Respir Cell Mol Biol 2012 Dec [PMID: 22865625]
• Du W, Wright BM, Li X, Finke J, Rini BI, Zhou M, He H, Lal P, Welford SM. Tumor-derived macrophage migration inhibitory factor promotes an autocrine loop that enhances renal cell carcinoma. Oncogene. 2012 Apr 30. [PMID: 22543583] (WB, Human)

Review for CD44 Antibody (NBP1-47386) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Corey. WB Human 07/12/2013

Summary

• Application: Western Blot
• Sample Tested: Human Brain Tissue
• Species: Human
• Lot: 130226
• Comments: Working in human tissue with non-specific signal at 50 kDa

Blocking

• Blocking Details: 5% Milk, 2 hours at RT

Primary Anitbody

• Dilution Ratio: 1:1000, TBST over night in cold room

Secondary Antibody

• Secondary Description: Goat anti Mouse/ HRP
• Secondary Manufacturer Cat#: Jackson Immuno
• Secondary Concentration: 1:10000

Details

• Detection Notes: ECL-Plus

Comments

• Comments: Working in human tissue with non-specific signal at 50 kDa

Bioinformatics

• Gene Symbol: CD44
• Entrez:
  • 960(Human)
  • 12505(Mouse)
  • 25406(Rat)
  • 100126860(Porcine)
• Uniprot:
  • P16070()