CD44 Antibody (8E2F3) - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye ...read more
Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of FFPE human breast carcinoma tissues using CD44 antibody (8E2F3). The signal was developed using DAB based detection and the sections were ...read more
Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left).
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Western Blot: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386] - Generation and characterization of immortalized primary keratinocytes (IPKs) from K14Cre.Ptpn2w/w and K14Cre.Ptpn2fl/fl mice. Western blot analysis of ...read more
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of methanol-fixed A431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. DRAQ5 ...read more
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of PANC-1 cells using anti-CD44 mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). DRAQ5 fluorescent DNA ...read more
Flow (Intracellular): CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Immunoprecipitation: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386] - HAP1 lysates were prepared and immunoprecipitation was performed using 1.0 ug of the CD44 Antibody (NBP1-47386) pre-coupled to either ...read more

Product Details

Summary
Reactivity Hu, Mu, Rb(-), Pm, Ca, EqSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP
Clone
8E2F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

CD44 Antibody (8E2F3) - BSA Free Summary

Immunogen
Purified recombinant fragment of human CD44 (628-699) expressed in E. coli. [Uniprot: P16070]
Localization
Membrane; Single-pass type I membrane protein.
Marker
Cell Membrane Marker
Predicted Species
Baboon (94%), Equine (93%), Canine (93%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD44
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow (Intracellular)
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation
  • Western Blot
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP1-47386 in the following applications:

Publications
Read Publications using
NBP1-47386 in the following applications:

Reactivity Notes

Not reactive to rabbit per customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for CD44 Antibody (8E2F3) - BSA Free

  • CD44 antigen
  • CD44 molecule (Indian blood group)
  • CD44
  • CD44R
  • CDw44
  • cell surface glycoprotein CD44
  • chondroitin sulfate proteoglycan 8
  • CSPG8
  • ECMR-III
  • epican
  • Extracellular matrix receptor III
  • GP90 lymphocyte homing/adhesion receptor
  • HCAM
  • HCELL
  • hematopoietic cell E- and L-selectin ligand
  • Heparan sulfate proteoglycan
  • Hermes antigen
  • homing function and Indian blood group system
  • HUTCH-I
  • Hyaluronate receptor
  • IN
  • LHR
  • MC56
  • MDU2
  • MDU2CD44 antigen (homing function and Indian blood group system)
  • MDU3
  • MDU3CDW44
  • MIC4
  • MIC4MGC10468
  • MUTCH-I
  • Pgp1
  • PGP-1
  • PGP-I
  • Phagocytic glycoprotein 1
  • Phagocytic glycoprotein I

Background

CD44, also known as IN, LHRMIC4, CDW44, HCELL, is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

7268-CT
Species: Hu
Applications: BA
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
796-IC
Species: Mu
Applications: BA
210-TA
Species: Hu
Applications: BA
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NB100-77417
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, In vivo, WB
728-LS
Species: Hu
Applications: BA
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
202-IL
Species: Hu
Applications: BA
NBP1-91258
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
MAB17781
Species: Hu
Applications: Block, CyTOF-ready, Flow, ICC, IP, KO
NB600-1071
Species: Hu(-), Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF1730
Species: Hu
Applications: AdBlk, CyTOF-ready, Flow, ICC
NB100-77903
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-Fr
NB500-328
Species: Hu
Applications: CyTOF-ready, Flow, IP
AF6585
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
AF808
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, IHC, Neut, WB
D6050
Species: Hu
Applications: ELISA
AF2364
Species: Hu
Applications: IHC, WB

Publications for CD44 Antibody (NBP1-47386)(17)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 7 applications: FLOW, Flow, ICC/IF, IHC, IHC-P, PAGE, WB.


Filter By Application
FLOW
(1)
Flow
(3)
ICC/IF
(2)
IHC
(3)
IHC-P
(4)
PAGE
(1)
WB
(4)
All Applications
Filter By Species
Human
(7)
Mouse
(6)
Rat
(1)
Canine
(1)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NBP1-47386 Applications Species
Lubanska D, Alrashed S, Mason GT et al. Impairing proliferation of glioblastoma multiforme with CD44+ selective conjugated polymer nanoparticles Scientific reports Jul 15 2022 [PMID: 35840697] (PAGE) PAGE
Byun Js, Oh M, Lee S Et Al. The transcription factor PITX1 drives astrocyte differentiation by regulating the SOX9 gene J. Biol. Chem. Aug 5 2020 [PMID: 32759168] (IHC, Rat) IHC Rat
Chen PY, Qin L, Li G et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms Cell Stem Cell Apr 2 2020 [PMID: 32243809] (Mouse) Mouse
Saxena N, Bhardwaj G, Jadhav S, et al. Pre-metastatic niche drives breast cancer invasion by modulating MSC homing and CAF differentiation bioRxiv Jan 15 2021 (ICC/IF, Human) ICC/IF Human
Li H, Chaitankar V, Zhu J et al. Olfactomedin 4 mediation of prostate stem/progenitor-like cell proliferation and differentiation via MYC Scientific reports Dec 14 2020 [PMID: 33318499] (IHC-P, ICC/IF, Human) IHC-P, ICC/IF Human
Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (Flow, Mouse)

Details:
Citation using the Allophycocyanin format of this antibody.
Flow Mouse
Tsai FJ, Lai MT, Cheng J et al. Novel K6-K14 keratin fusion enhances cancer stemness and aggressiveness in oral squamous cell carcinoma Oncogene Mar 13 2019 [PMID: 30867567] (IHC, Mouse) IHC Mouse
Scimeca M, Urbano N, Bonfiglio R et al. Breast osteoblast-like cells: a new biomarker for the management of breast cancer. Br. J. Cancer. Oct 17 2018 [PMID: 30327566] (IHC-P, Human) IHC-P Human
Marzban H, Sasani F Canine Mammary Gland Cancer Stem Cell and its Potential Role in Malignant Biologic Behavior Iranian Journal of Veterinary Medicine Jan 1 2020 (WB, Canine) WB Canine
Hassanzadeh H, Matin MM, Naderi-Meshkin H et al. Using paracrine effects of Ad-MSCs on keratinocyte cultivation and fabrication of epidermal sheets for improving clinical applications Cell Tissue Bank Aug 13 2018 [PMID: 30105667] (Flow, Human) Flow Human
Show All 17 Publications.

Review for CD44 Antibody (NBP1-47386) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-47386:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot CD44 NBP1-47386
Enlarge
5
reviewed by:
Verified Customer
WB Human 07/12/2013
View

Summary

ApplicationWestern Blot
Sample TestedHuman Brain Tissue
SpeciesHuman
Lot130226

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD44 Antibody (NBP1-47386) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional CD44 Products

Bioinformatics Tool for CD44 Antibody (NBP1-47386)

Discover related pathways, diseases and genes to CD44 Antibody (NBP1-47386). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CD44 Antibody (NBP1-47386)

Discover more about diseases related to CD44 Antibody (NBP1-47386).
 

Pathways for CD44 Antibody (NBP1-47386)

View related products by pathway.

PTMs for CD44 Antibody (NBP1-47386)

Learn more about PTMs related to CD44 Antibody (NBP1-47386).
 

Research Areas for CD44 Antibody (NBP1-47386)

Find related products by research area.

Blogs on CD44.

Taking Biomarker Discovery From 2D to 3D: Increased Biological Activity of EVs Isolated From 3D Prostate Cancer Cultures
Jamshed Arslan, Pharm D, PhD Tissues within the human body are made of a three-dimensional (3D) arrangement of cells working together to perform vital functions. The commonly used 2D monolayer cultures have limited ...  Read full blog post.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

The Osteopontin Antibody and Hepatic Research
Antibody suppliers, such as us at Novus Biologicals, supply a wide range of cell marker products, among them the Osteopontin antibody. Recently, the osteopontin antibody has proven useful in hepatic cancer research.Osteopontin (OPN) is mainly expres...  Read full blog post.

CD Antibodies Uncover Markers for Rare Breast Cancer
We at Novus Biologicals have added several new products to our CD antibody database. The CD, or Cluster of Differential proteins are a family of type I transmembrane glycoproteins widely expressed in immune cell populations. These include B cells, thy...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
07/12/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol CD44
Entrez
Uniprot