Western Blot: CD44 Antibody (8E2F3) [NBP1-47386] - Western blot analysis using anti-CD44 mAb against Hela (1) and HUVE-12 (2) cell lysates.
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Confocal analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 ...read more
Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using anti-CD44 mAb.
Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Flow cytometric analysis of Hela cells using anti-CD44 mAb (right) and negative control (left).
Western Blot: CD44 Antibody (8E2F3) [NBP1-47386] - Western blot analysis using anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Confocal immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing ...read more
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Confocal immunofluorescence analysis of PANC-1 cells using anti-CD44 mAb (green). Red: Actin filaments have been labeled with DY-554 ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.03% Sodium Azide
Ammonium sulfate precipitation
This CD44 (8E2F3) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP1-47386 in the following application:
Not reactive to rabbit per customer review. Immunogen's sequence homology with other species: Bovine (89%), Rat (87%), Golden hamster (87%) and Chinese hamster (80%)
Alternate Names for CD44 Antibody (8E2F3)
CD44 molecule (Indian blood group)
cell surface glycoprotein CD44
chondroitin sulfate proteoglycan 8
Extracellular matrix receptor III
GP90 lymphocyte homing/adhesion receptor
hematopoietic cell E- and L-selectin ligand
Heparan sulfate proteoglycan
homing function and Indian blood group system
MDU2CD44 antigen (homing function and Indian blood group system)
Phagocytic glycoprotein 1
Phagocytic glycoprotein I
CD44, also known as IN, LHRMIC4, CDW44, HCELL, is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.