CD44 Antibody (8E2F3)

Images

 
Western Blot: CD44 Antibody (8E2F3) [NBP1-47386] - Western blot analysis using anti-CD44 mAb against Hela (1) and HUVE-12 (2) cell lysates.
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Confocal analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 ...read more
Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human breast carcinoma tissues using CD44 antibody (clone 8E2F3). The signal was developed ...read more
Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Flow cytometric analysis of Hela cells using anti-CD44 mAb (right) and negative control (left).
Western Blot: CD44 Antibody (8E2F3) [NBP1-47386] - Western blot analysis using anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Confocal immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing ...read more
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Confocal immunofluorescence analysis of PANC-1 cells using anti-CD44 mAb (green). Red: Actin filaments have been labeled with DY-554 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rb(-), Bb, Ca, EqSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
8E2F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CD44 Antibody (8E2F3) Summary

Immunogen
Purified recombinant fragment of human CD44 (628-699) expressed in E. coli. [UniProt# P16070]
Localization
Membrane; Single-pass type I membrane protein.
Marker
Cell Membrane Marker
Predicted Species
Canine (93%), Equine (93%), Baboon (94%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD44
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This CD44 (8E2F3) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-47386 in the following applications:

Publications
Read Publications using
NBP1-47386 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • 1 publication
  • WB
    2 publications

Reactivity Notes

Not reactive to rabbit per customer review. Immunogen's sequence homology with other species: Bovine (89%), Rat (87%), Golden hamster (87%) and Chinese hamster (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for CD44 Antibody (8E2F3)

  • CD44 antigen
  • CD44 molecule (Indian blood group)
  • CD44
  • CD44R
  • CDw44
  • cell surface glycoprotein CD44
  • chondroitin sulfate proteoglycan 8
  • CSPG8
  • ECMR-III
  • epican
  • Extracellular matrix receptor III
  • GP90 lymphocyte homing/adhesion receptor
  • HCAM
  • HCELL
  • hematopoietic cell E- and L-selectin ligand
  • Heparan sulfate proteoglycan
  • Hermes antigen
  • homing function and Indian blood group system
  • HUTCH-I
  • Hyaluronate receptor
  • IN
  • LHR
  • MC56
  • MDU2
  • MDU2CD44 antigen (homing function and Indian blood group system)
  • MDU3
  • MDU3CDW44
  • MIC4
  • MIC4MGC10468
  • MUTCH-I
  • Pgp1
  • PGP-1
  • PGP-I
  • Phagocytic glycoprotein 1
  • Phagocytic glycoprotein I

Background

CD44, also known as IN, LHRMIC4, CDW44, HCELL, is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD44 Antibody (NBP1-47386)(4)

Review for CD44 Antibody (NBP1-47386) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-47386:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot CD44 NBP1-47386
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reviewed by:
Corey.
WB Human 07/12/2013
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Summary

ApplicationWestern Blot
Sample TestedHuman Brain Tissue
SpeciesHuman
Lot130226
CommentsWorking in human tissue with non-specific signal at 50 kDa

Blocking

Blocking Details5% Milk, 2 hours at RT

Primary Anitbody

Dilution Ratio1:1000, TBST over night in cold room

Secondary Antibody

Secondary DescriptionGoat anti Mouse/ HRP
Secondary Manufacturer Cat#Jackson Immuno
Secondary Concentration1:10000

Details

Detection NotesECL-Plus

Comments

CommentsWorking in human tissue with non-specific signal at 50 kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD44 Antibody (NBP1-47386) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CD44 Antibody (NBP1-47386)

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Research Areas for CD44 Antibody (NBP1-47386)

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Blogs on CD44.

The Osteopontin Antibody and Hepatic Research
Antibody suppliers, such as us at Novus Biologicals, supply a wide range of cell marker products, among them the Osteopontin antibody. Recently, the osteopontin antibody has proven useful in hepatic cancer research.Osteopontin (OPN) is mainly expres...  Read full blog post.

CD Antibodies Uncover Markers for Rare Breast Cancer
We at Novus Biologicals have added several new products to our CD antibody database. The CD, or Cluster of Differential proteins are a family of type I transmembrane glycoproteins widely expressed in immune cell populations. These include B cells, thy...  Read full blog post.

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Recent Reviews

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1
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Corey.
07/12/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol CD44
Entrez
Uniprot