CD44 Antibody (8E2F3) - Azide and BSA Free

Western Blot: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - Analysis using anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2). Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue). Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - Analysis of FFPE human breast carcinoma tissues using CD44 antibody (8E2F3). The signal was developed using DAB based detection and the sections were processed for counterstaining with hematoxylin. The antibody generated mainly a membrane staining represe

Flow Cytometry: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left). Image from the standard format of this antibody.

Western Blot: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - Analysis using anti-CD44 mAb against HeLa (1) and HUVE-12 (2) cell lysates. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - Analysis of methanol-fixed A431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue). Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - Analysis of PANC-1 cells using anti-CD44 mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). DRAQ5 fluorescent DNA dye (blue). Image from the standard format of this antibody.

Flow Cytometry: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - An intracellular stain was performed on HeLa cells with NBP1-47386AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.

• Reactivity: Hu, Mu, Rb(-)
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 8E2F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD44 Antibody (8E2F3) - Azide and BSA Free Summary

• Immunogen: Purified recombinant fragment of human CD44 (628-699) expressed in E. coli. [Uniprot: P16070]
• Localization: Membrane; Single-pass type I membrane protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD44
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow (Intracellular)
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Not reactive to rabbit per customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for CD44 Antibody (8E2F3) - Azide and BSA Free

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44, also known as IN, LHRMIC4, CDW44, HCELL, is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD44