A Multicolored Approach to CD4 Antibody Assays

Thu, 02/23/2012 - 16:04

Novus Biologicals offers the widest selection of conjugated CD4 antibody products, with a database that's continually expanding to take into account the latest developments. This includes CD4 antibodies conjugated to Dynomics and PE fluorescent dyes, for FACS analysis.

CD4 is a cluster of differentiation transmembrane protein belonging to the immunoglobulin superfamily (IgSF), a large group of proteins with functions in the recognition, adhesion and binding of cells. CD4 is widely expressed on T helper lymphocytes, and to a lesser degree on monocytes, macrophages, and dendritic cells.

Immunohistochemistry-Paraffin: CD4 Antibody

Conjugated antibodies are those which are chemically bound to a dye; either chromatic (chromogen) or fluorescent (fluorochrome) to allow visual detection of the antigen for quantitative analysis. Conjugated CD4 antibodies are widely used as stem cell markers, in a variety of assays. Applications vary depending on the conjugate, but include immunoprecipitation (IP); immunohistochemistry (IHC); immunofluorescence; flow cytometry (FCM) analysis. FACS (fluorescence-activated cell sorting) is a specialized form of FCM, used in, for example, the sorting of stem cells for cloning.

Our conjugated CD4 antibodies include a number of fluorochrome-conjugated reagents, for example our Phycoerythrin (PE) antibodies, which activate and fluoresce (emit light) at specific wavelengths.

PE is a large protein derived from algae, and one of the most commonly used FACS dyes. The dye has a large absorption coefficient, and even though only one PE molecule is normally conjugated to each CD4 antibody, it is one of the brightest dyes in use today. It can be excited by common Argon laser tuned to 488 nm; although some users prefer a 533nm setting, which is more sensitive. It emits at around 570 nm.

Novus Biologicals offers CD4 reagents for your research needs including:

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