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CD4 Antibodies

CD4 (cluster of differentiation 4) is a single chain type I transmembrane glycoprotein belonging to the immunoglobin (Ig) superfamily. CD4 has a theoretical molecular weight of 55 kDa and is expressed on most thymocytes and a subset of mature T lymphocytes. CD4 functions in T cell development and has a diverse role in innate immunity. We offer highly rated antibodies to detect CD4 that have been cited in 80+ publications. Browse our extensive catalog for recombinant monoclonal antibodies, monoclonal antibodies, polyclonal antibodies, and CD4-conjugated antibodies. Validated research applications for our CD4 antibodies include Blocking/Neutralizing, CyTOF-ready, ELISA, Enzyme Immunoassay, Flow Cytometry, ICC, Immunofluorescence, IHC-Frozen, IHC-Paraffin, Immunoprecipitation, Simple Western, and Western Blot. Our CD4 antibodies are used in a variety of model species including Human, Mouse, Rat, Bovine, Chicken, Feline, Goat, Primate, Rabbit, Sheep, and more. Choose the anti-CD4 antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products. Each CD4 antibody is fully covered by Novus’ Guarantee+ to give you peace of mind and support when you need it.

Alternate Names for CD4 Antibodies

anti-CD4 antibody, anti-CD4 antigen (p55) antibody, anti-CD4 antigen antibody, anti-CD4 molecule antibody, anti-CD4 receptor antibody, anti-CD4mut antibody, anti-T-cell surface antigen T4/Leu-3 antibody, anti-T-cell surface glycoprotein CD4 antibody

153 Results for "cd4" in Primary Antibodies

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NBP1-19371

CD4 Antibody - BSA Free

(9 Reviews)

(53 Publications)

0.1 ml

NBP1-19371

Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.

Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).

Flow Cytometry: CD4 Antibody - BSA Free [NBP1-19371] - An intracellular stain was performed on U-251 MG cells with CD4 Antibody NBP1-19371AF594 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Western Blot: CD4 Antibody [NBP1-19371] - Mouse spleen, 25ug total protein. Image from verified customer review.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Staining of paraffin-embedded mouse brain tissue. Photo courtesy of product review by verified customer.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Analysis of a FFPE tissue section of mouse brain using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody primarily generated a strong signal in the membranes of a subset of cells in the tested section.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Analysis of a FFPE section of mouse spleen using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong signal in the membranes of a subset of cells in the spleen section and the staining showed punctate appearance in some cells.

Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on U937 cells with CD4 Antibody NBP1-19371AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on U937 cells with CD4 Antibody NBP1-19371G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.

Simple Western: CD4 Antibody [NBP1-19371] - Lane view shows a specific band for CD4 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Dual RNAscope ISH-IHC: CD4 Antibody [NBP1-19371] - IFNG mRNA (red) and CD4 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human lung cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-IFNG and CD4 antibody at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin (Cat. No. DS9800), BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).

Dual RNAscope ISH-IHC: CD4 Antibody [NBP1-19371] - CCR5 mRNA (red) and CD4 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human lung cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CCR5 and CD4 antibody at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).

ATM activation by chloroquine alleviates senescence.(a) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in human skin fibroblasts (HSFs). A gradually increased level of p16 indicates cellular senescence, while elevated gamma H2AX level indicates accumulated DNA damage. (b) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in mouse embryonic fibroblasts (MEFs). (c) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in brain tissues isolated from 3-, 10-, and 18-month-old male mice. (d) SA-beta -Gal staining in HSFs treated with sh-ATM or scramble shRNA. Scale bar, 100 µm. (e) Quantification of SA-beta -Gal-positive staining of (d) from five views randomly captured for each group. Data represent means ± SEM. ***p<0.001. (f) Immunoblots showing increased gamma H2AX and unaffected LC3I/II in HSFs treated with sh-ATM or scramble shRNA. (g) Immunoblots showing protein levels of pS1981 ATM, gamma H2AX, and cleaved caspase-3 in HSFs treated with 10 μM of CQ for indicated time. (h) SA-beta -Gal staining in HSFs expressing either scramble or ATM shRNA treated with 1 μM CQ or DMSO (12 hr). Scale bar, 100 µm. (i) Quantification of SA-beta -Gal-positive staining of (h) from five views randomly captured for each group. Data represent means ± SEM. ***p<0.001; ‘N.S.’ indicates no significant difference. (j) HSFs at passage 20 were continuously cultured with 1 μM CQ or DMSO, and cell number was calculated at each passage. Data represent means ± SEM. ***p<0.01. (k) Immunoblots showing protein levels of gamma H2AX, p62, and LC3 in MEFs treated with 1 μM CQ or DMSO. Note that CQ had little effect on the expression levels of p62 and LC3. (l) MEFs at passage one were continuously cultured in 20% O2 with 1 μM CQ or DMSO, and cell number was determined at each passage. Data represent means ± SEM. ***p<0.01.10.7554/eLife.34836.006Figure 1—source data 1.Statistical analysis for SA-beta -Gal positive staining.10.7554/eLife.34836.007Figure 1—source data 2.Statistical analysis for EdU positive staining.Sta

Immunocytochemistry/ Immunofluorescence: CD4 Antibody - BSA Free [NBP1-19371] - Rats with mild liver damage show immune cells infiltration in cerebellum at 4 weeks, which is prevented by rifaximin. Analysis of immune cells infiltration in cerebellum was performed at 4 weeks by immunohistochemistry using antibodies against (A) CD4, a marker of T lymphocytes (F(3,8) = 4.511, p < 0.01) & (B) IBA1, a marker of meningeal & perivascular macrophages (K-W(4,10) = 27.76, p < 0.0001). (C) Double immunofluorescence with anti-CD4 & anti-CX3CR1 as markers of autoreactive CD4+CD28− T lymphocytes was performed & quantified (K-W(4,5) = 8.711, p < 0.05). (D) Double immunofluorescence with anti-CD4 & anti-CCR6 as marker of Th17 lymphocytes was performed & quantified (K-W(4,7) = 8.858, p < 0.05). Number of animals in each group was added under group names. One-way ANOVA with Tukey’s test (CD4+) & nonparametric Kruskal–Wallis (K-W statistic) with Dunn’s test (Iba1, CD4+/CX3CR1+ & CD4+/CCR6+) was performed to compare all groups. Values significantly different from control rats are indicated by asterisks & from CCl4 rats by a. * p < 0.05, a p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34440206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Topical 2% REB decreases the ocular inflammatory cytokines in PM-induced ocular surface inflammation in a rat model. (A) Immunofluorescence staining for (i) MMP-9, (ii) TNF-alpha , (iii) IL-1 beta , and (iv) IL-17 in the cornea of normal, PM-exposed, PM+REB, and PM+HA-treated groups. (B) Western blot analysis of pNF-kappa B and NF-kappa B, (C) IL-17, (D) CD-4, and (E) MMP-9 expression in the conjunctiva. (F) Changes in the expression of MMP-9, (G) TNF-alpha , (H) IL-1 beta , and (I) IL-17 in the cornea. (J) Relative expression of pNF-kappa B, (K) IL-17, (L) CD-4, and (M) MMP-9 normalized to GAPDH. In (F–M), the data are expressed as mean +/- SEM (n = 6, n = 4). One-way ANOVA was followed by the Tukey test. * p < 0.05, ** p < 0.01, *** p < 0.001 indicate statistically significant differences. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40332777), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immune signaling in the WAT during CoV-2 infection is altered differently between the sexes. Immunoblot images of (a) immune cell markers (CD4, CD8, F4/80) and (b) inflammatory markers (TNF alpha , IL-6, IL-10). beta -actin was used as loading control. Fold changes in the protein levels were normalized to beta -actin expression and are shown as bar graphs. The error bars represent standard error of the mean. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared to uninfected sex-matched mice and between the infected groups (n = 4/sex/group) (M, male; F, female). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36674830), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Rats with mild liver damage show immune cells infiltration in cerebellum at 4 weeks, which is prevented by rifaximin. Analysis of immune cells infiltration in cerebellum was performed at 4 weeks by immunohistochemistry using antibodies against (A) CD4, a marker of T lymphocytes (F(3,8) = 4.511, p < 0.01) and (B) IBA1, a marker of meningeal and perivascular macrophages (K-W(4,10) = 27.76, p < 0.0001). (C) Double immunofluorescence with anti-CD4 and anti-CX3CR1 as markers of autoreactive CD4+CD28− T lymphocytes was performed and quantified (K-W(4,5) = 8.711, p < 0.05). (D) Double immunofluorescence with anti-CD4 and anti-CCR6 as marker of Th17 lymphocytes was performed and quantified (K-W(4,7) = 8.858, p < 0.05). Number of animals in each group was added under group names. One-way ANOVA with Tukey’s test (CD4+) and nonparametric Kruskal–Wallis (K-W statistic) with Dunn’s test (Iba1, CD4+/CX3CR1+ and CD4+/CCR6+) was performed to compare all groups. Values significantly different from control rats are indicated by asterisks and from CCl4 rats by a. * p < 0.05, a p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34440206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

(A) Immunofluorescence staining showing the expression of (i) PD-L1, (ii) CD4, (iii) CD3e and (iv) IL-17 localized in the corneas in the control, desiccation+PBS and desiccation+PD-L1 group. (B–E) Changes in the percentage of PD-L1, CD4, CD3e and IL-17 positive cells in the cornea. Immunopositivity for PD-L1 is shown in higher-power fields. Immunopositivity was counted in low-power fields and calculated as relative to the total number of DAPI-positive cells. (F,G) Western blot showing the expression of CD4 and IL-17 in the cornea. (H,I) Relative expression ratio of CD4 and IL-17 to beta -actin. In (B–E,H,I), data are presented with bar graph (n = 6, day 10, * p < 0.05; ** p < 0.01; *** p < 0.001 significant difference by one-way ANOVA). Original images of (F,G) can be found in Supplementary Materials. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/2218-273X/14/1/68), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

RD and HFD differently regulate the expression levels of p-AMPK alpha , p-mTOR, p-Perilipin 1, CD4, CD8, F4/80, TNF alpha , IFN gamma , and IL-6 in tumor-associated mammary fat pad during early (1 wpi) and later (5 wpi) time points of tumor growth in young female E0771 tumor-bearing mice. (A) Immunoblots showing different protein expression between RD and HFD at 1 wpi and 5 wpi. beta -Actin was used as loading control. (B) Bar graphs showing the levels of each protein marker normalized to beta -Actin (top panel—1 wpi; bottom panel—5 wpi). The error bars represent the standard error of the mean. * p ≤ 0.05 and *** p ≤ 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36675341), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Ca, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ca, Rb

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-19371AF350
Alexa Fluor 405	NBP1-19371AF405
Alexa Fluor 488	NBP1-19371AF488
Alexa Fluor 532	NBP1-19371AF532
Alexa Fluor 594	NBP1-19371AF594
Alexa Fluor 647	NBP1-19371AF647
Alexa Fluor 700	NBP1-19371AF700
Alexa Fluor 750	NBP1-19371AF750
Alexa Fluor Plus 405	NBP1-19371AFP405
Alexa Fluor Plus 488	NBP1-19371AFP488
Alexa Fluor Plus 555	NBP1-19371AFP555
Alexa Fluor Plus 594	NBP1-19371AFP594
Alexa Fluor Plus 647	NBP1-19371AFP647
Alexa Fluor Plus 680	NBP1-19371AFP680
Allophycocyanin	NBP1-19371APC
Biotin	NBP1-19371B
CoraFluor 1	NBP1-19371CL1
DyLight 350	NBP1-19371UV
DyLight 405	NBP1-19371V
DyLight 488	NBP1-19371G
DyLight 550	NBP1-19371R
DyLight 594	NBP1-19371DL594
DyLight 650	NBP1-19371C
DyLight 680	NBP1-19371FR
DyLight 755	NBP1-19371IR
FITC	NBP1-19371F
HRP	NBP1-19371H
Janelia Fluor 525	NBP1-19371JF525
Janelia Fluor 549	NBP1-19371JF549
Janelia Fluor 646	NBP1-19371JF646
Janelia Fluor 669	NBP1-19371JF669
PE	NBP1-19371PE
PerCP	NBP1-19371PCP
mFluor Violet 450 SE	NBP1-19371MFV450
mFluor Violet 500 SE	NBP1-19371MFV500
mFluor Violet 610 SE	NBP1-19371MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-19371SS

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AF-379-NA

CD4 Antibody [Unconjugated]

(1 Review)

(31 Publications)

100 μg

AF-379-NA

Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

CD4 was detected in immersion fixed human T cells using 2 µg/mL Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑379‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for <a class=NoLineLink href=

Fluorescent ICC Staining of Cells on Coverslips." alt="CD4 was detected in immersion fixed human T cells using 2 µg/mL Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑379‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; <a class=

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol." class="big_lightbox">

CD4 was detected in immersion fixed paraffin-embedded sections of human tonsil tissue using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=

HAF017). A specific band was detected for CD4 at approximately ~55kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of PBMC and SUP‑T1 human T cell lymphoblastic lymphoma cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Simple Western lane view shows lysates of Human PBMC and SUP‑T1 human T cell lymphoblastic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD4 at approximately 65 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-379-NAU-100UG
Alexa Fluor 405	AF-379-NAV-100UG
Alexa Fluor 488	FAB8165G
Alexa Fluor 532	AF-379-NAX-100UG
Alexa Fluor 594	AF-379-NAT-100UG
Alexa Fluor 647	AF-379-NAR-100UG
Alexa Fluor 700	FAB8165N-100
Alexa Fluor 750	AF-379-NAS-100UG
Alexa Fluor Plus 405	AF-379-NAAFP405
Alexa Fluor Plus 488	AF-379-NAAFP488
Alexa Fluor Plus 555	AF-379-NAAFP555
Alexa Fluor Plus 594	AF-379-NAAFP594
Alexa Fluor Plus 647	AF-379-NAAFP647
Alexa Fluor Plus 680	AF-379-NAAFP680
Allophycocyanin	AF-379-NAA
Biotin	BAF379
CoraFluor 1	AF-379-NACL1
DyLight 350	AF-379-NAD
DyLight 405	AF-379-NAE
DyLight 488	AF-379-NAK
DyLight 550	AF-379-NAL
DyLight 594	AF-379-NAM
DyLight 650	AF-379-NAW
DyLight 680	AF-379-NAY
DyLight 755	AF-379-NAZ
FITC	AF-379-NAF
HRP	AF-379-NAH
Janelia Fluor 525	AF-379-NAJF525
Janelia Fluor 549	AF-379-NAI
Janelia Fluor 646	AF-379-NAJ
Janelia Fluor 669	AF-379-NAJF669
PE	AF-379-NAP
PerCP	AF-379-NAC
mFluor Violet 450 SE	AF-379-NAMFV450
mFluor Violet 500 SE	AF-379-NAMFV500
mFluor Violet 610 SE	AF-379-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB8165G
Carrier Free	AF-379-SP
Carrier Free	FAB8165N-100

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NBP2-25199

CD4 Antibody (RPA-T4) - Azide and BSA Free

(1 Review)

(13 Publications)

0.1 mg

NBP2-25199

Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the FITC conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis. Image using the Azide Free format of this antibody.

Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the DyLight 405 conjugate of NBP2-27216. Staining of CD4 in human PBMCs using anti-CD4 antibody. Image from verified customer review.

Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of this antibody. Green represents isotype control; red represents anti-CD4 antibody. Image using the Azide Free format of this antibody.

Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Allophycocyanin conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis. Image using the Azide Free format of this antibody.

Flow Cytometry: CD4 Antibody (RPA-T4) - Azide and BSA Free [NBP2-25199] - analysis of CD4 in human PBMCs using 0.1 ug of this antibody. Secondary antibody is goat anti-mouse PE.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-5916A

Alt Catalog# IMG-5916A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-27216AF350
Alexa Fluor 405	NBP2-27216AF405
Alexa Fluor 488	NBP2-27216AF488
Alexa Fluor 532	NBP2-27216AF532
Alexa Fluor 594	NBP2-27216AF594
Alexa Fluor 647	NBP2-27216AF647
Alexa Fluor 700	NBP2-27216AF700
Alexa Fluor 750	NBP2-27216AF750
Alexa Fluor Plus 405	NBP2-27216AFP405
Alexa Fluor Plus 488	NBP2-27216AFP488
Alexa Fluor Plus 555	NBP2-27216AFP555
Alexa Fluor Plus 594	NBP2-27216AFP594
Alexa Fluor Plus 647	NBP2-27216AFP647
Alexa Fluor Plus 680	NBP2-27216AFP680
Allophycocyanin	NBP2-27245
Biotin	NBP2-25199B
DyLight 350	NBP2-27216UV
DyLight 405	NBP2-27216V
DyLight 488	NBP2-27216G
DyLight 550	NBP2-27216R
DyLight 650	NBP2-27216C
DyLight 680	NBP2-27216FR
DyLight 755	NBP2-27216IR
FITC	NBP2-27216F
HRP	NBP2-27216H
Janelia Fluor 549	NBP2-27216JF549
Janelia Fluor 646	NBP2-27216JF646
PE	NBP2-27248
PerCP	NBP2-27216PCP
mFluor Violet 450 SE	NBP2-27216MFV450
mFluor Violet 500 SE	NBP2-27216MFV500
mFluor Violet 610 SE	NBP2-27216MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-27216
Azide and BSA Free	NBP2-25199SS
Azide and BSA Free	NBP2-27216-25ug

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NBP2-25191

CD4 Antibody (GK1.5) - BSA Free

(7 Publications)

0.1 mg

NBP2-25191

Flow Cytometry: CD4 Antibody (GK1.5) [NBP2-25191] - Analysis using the Allophycocyanin conjugate of NBP2-26585. Staining of CD4 in 10^6 BALB/c mouse splenocytes using 1 ug of this antibody. Green represents isotype control; red represents mouse CD4 this antibody. Image using the Azide Free format of this antibody.

Flow Cytometry: CD4 Antibody (GK1.5) [NBP2-25191] - Analysis using the FITC conjugate of NBP2-26585. Staining of CD4 in 10^6 BALB/c mouse splenocytes using 1 ug of NBP2-26584. Green represents isotype control (20212C); red represents mouse CD4 NBP2-26584 antibody. Image using the Azide Free format of this antibody.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Alt Catalog#

IMG-5922A

Alt Catalog# IMG-5922A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25191AF350
Alexa Fluor 405	NBP2-25191AF405
Alexa Fluor 488	NBP2-25191AF488
Alexa Fluor 532	NBP2-25191AF532
Alexa Fluor 594	NBP2-25191AF594
Alexa Fluor 647	NBP2-25191AF647
Alexa Fluor 700	NBP2-25191AF700
Alexa Fluor 750	NBP2-25191AF750
Alexa Fluor Plus 405	NBP2-25191AFP405
Alexa Fluor Plus 488	NBP2-25191AFP488
Alexa Fluor Plus 555	NBP2-25191AFP555
Alexa Fluor Plus 594	NBP2-25191AFP594
Alexa Fluor Plus 647	NBP2-25191AFP647
Alexa Fluor Plus 680	NBP2-25191AFP680
Allophycocyanin	NBP2-26598
Allophycocyanin/Cy7	NBP2-25191APCCY7
Biotin	NBP2-25191B
CoraFluor 1	NBP2-25191CL1
DyLight 350	NBP2-25191UV
DyLight 405	NBP2-25191V
DyLight 488	NBP2-25191G
DyLight 550	NBP2-25191R
DyLight 594	NBP2-25191DL594
DyLight 650	NBP2-25191C
DyLight 680	NBP2-25191FR
DyLight 755	NBP2-25191IR
FITC	NBP2-25191F
HRP	NBP2-25191H
Janelia Fluor 525	NBP2-25191JF525
Janelia Fluor 549	NBP2-25191JF549
Janelia Fluor 646	NBP2-25191JF646
Janelia Fluor 669	NBP2-25191JF669
PE	NBP2-25191PE
PE/Atto594	NBP2-25191PEATT594
PE/Cy5.5	NBP2-25191PECY55
PE/Cy7	NBP2-25191PECY7
PerCP	NBP2-25191PCP
mFluor Violet 450 SE	NBP2-25191MFV450
mFluor Violet 500 SE	NBP2-25191MFV500
mFluor Violet 610 SE	NBP2-25191MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-26585
BSA Free	NBP2-25191SS
Azide and BSA Free	NBP2-26585-1mg
Azide and BSA Free	NBP2-26585-25ug

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NB100-64988

CD4 Antibody (W3/25) - BSA Free

(3 Publications)

0.25 mg

NB100-64988

Immunocytochemistry/Immunofluorescence: CD4 Antibody (W3/25) [NB100-64988] - Staining of rat splenocytes with CD4 (W3/25) antibody. Live cells were incubated with CD4 antibody on ice, then fixed with 2% formaldehyde prior to secondary antibody incubation.

Immunohistochemistry-Frozen: CD4 Antibody (W3/25) [NB100-64988] - Staining of frozen rat spleen with Mouse anti Rat CD4.

Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Staining of stimulated rat spleen cells.

Reactivity:

Species Glossary

Reactivity: Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-80647AF350
Alexa Fluor 405	NB100-64988AF405
Alexa Fluor 488	NB100-64988AF488
Alexa Fluor 532	NBP2-80647AF532
Alexa Fluor 594	NBP2-80647AF594
Alexa Fluor 647	NB100-64988AF647
Alexa Fluor 700	NB100-64988AF700
Alexa Fluor 750	NBP2-80647AF750
Allophycocyanin	NB100-64988APC
Biotin	NB100-64988B
DyLight 350	NB100-64988UV
DyLight 405	NB100-64988V
DyLight 488	NB100-64988G
DyLight 550	NB100-64988R
DyLight 594	NBP2-80647DL594
DyLight 650	NB100-64988C
DyLight 680	NB100-64988FR
DyLight 755	NB100-64988IR
FITC	NB100-64988F
HRP	NB100-64988H
Janelia Fluor 549	NBP2-80647JF549
Janelia Fluor 646	NBP2-80647JF646
PE	NB100-64988PE
PerCP	NB100-64988PCP

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-64988SS
Azide and BSA Free	NBP2-80647

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MAB379

CD4 Antibody (34930) [Unconjugated]

(11 Publications)

100 μg

MAB379

NL009) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB379) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # <a class=

NL009) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB379) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

CX3CR1 expressions in affected muscle of patients with polymyositis. The muscle tissues of polymyositis (PM) patients were double-stained with CD4, CD8, or CD68, as well as CX3CR1, and were analyzed with fluorescent microscopy: (A) CX3CR1, (B) CD4, (C) merged (A) and (B), (D) CX3CR1, (E) CD8, (F) merged (D) and (E), (G) CX3CR1, (H) CD68, and (I) merged (G) and (H). Arrows indicate double-positive cells. Original magnification, ×400. Image collected and cropped by CiteAb from the following publication (//arthritis-research.biomedcentral.com/articles/10.1186/ar3761), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB379U-100UG
Alexa Fluor 405	FAB379V-100UG
Alexa Fluor 488	FAB379G-100UG
Alexa Fluor 532	FAB379X
Alexa Fluor 594	FAB379T-100UG
Alexa Fluor 647	FAB379R-100UG
Alexa Fluor 700	FAB379N-100UG
Alexa Fluor 750	FAB379S-100UG
Alexa Fluor Plus 405	FAB379AFP405-100UG
Alexa Fluor Plus 488	FAB379AFP488-100UG
Alexa Fluor Plus 555	FAB379AFP555-100UG
Alexa Fluor Plus 594	FAB379AFP594-100UG
Alexa Fluor Plus 647	FAB379AFP647-100UG
Alexa Fluor Plus 680	FAB379AFP680-100UG
Allophycocyanin	FAB379A
Allophycocyanin/Cy7	FAB379APCCY7
Biotin	FAB379B
CoraFluor 1	FAB379CL1
DyLight 350	FAB379D
DyLight 405	FAB379E
DyLight 488	FAB379K
DyLight 550	FAB379L
DyLight 594	FAB379M
DyLight 650	FAB379W
DyLight 680	FAB379Y
DyLight 755	FAB379Z
FITC	FAB379F
HRP	FAB379H
Janelia Fluor 525	FAB379JF525
Janelia Fluor 549	FAB379I
Janelia Fluor 646	FAB379J
Janelia Fluor 669	FAB379JF669
PE	FAB379P
PE/Atto594	FAB379PEATT594
PE/Cy5.5	FAB379PECY55
PE/Cy7	FAB379PECY7
PerCP	FAB379C
mFluor Violet 450 SE	FAB379MFV450
mFluor Violet 500 SE	FAB379MFV500
mFluor Violet 610 SE	FAB379MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB379-SP
Carrier Free	MAB379-500
Carrier Free	FAB379V-100UG
Carrier Free	FAB379U-100UG
Carrier Free	FAB379T-100UG
Carrier Free	FAB379S-100UG
Carrier Free	FAB379R-100UG
Carrier Free	FAB379N-100UG
Carrier Free	FAB379G-100UG

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NBP2-76423

CD4 Antibody (BL-155-1C11) - BSA Free

100 ul

NBP2-76423

Flow Cytometry- CD4 Antibody (BL-155-1C11) [NBP2-76423] -Rabbit anti-CD4 recombinantmonoclonal [BL-155-1C11] or isotype control (unshaded). Secondary: DyLight® 488-conjugated goatanti-rabbit IgG.

Immunohistochemistry-Paraffin: CD4 Antibody (BL-155-1C11) [NBP2-76423] -Rabbit anti-CD4 recombinant monoclonal antibody[BL-155-1C11]. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate: DAB.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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AF554

CD4 Antibody [Unconjugated]

(10 Publications)

100 μg

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF554U-100UG
Alexa Fluor 405	AF554V-100UG
Alexa Fluor 488	AF554G-100UG
Alexa Fluor 532	AF554X-100UG
Alexa Fluor 594	AF554T-100UG
Alexa Fluor 647	AF554R-100UG
Alexa Fluor 700	AF554N-100UG
Alexa Fluor 750	AF554S-100UG
Alexa Fluor Plus 405	AF554AFP405-100UG
Alexa Fluor Plus 488	AF554AFP488-100UG
Alexa Fluor Plus 555	AF554AFP555-100UG
Alexa Fluor Plus 594	AF554AFP594-100UG
Alexa Fluor Plus 647	AF554AFP647-100UG
Alexa Fluor Plus 680	AF554AFP680-100UG
Allophycocyanin	AF554A
Biotin	BAF554
CoraFluor 1	AF554CL1
DyLight 350	AF554D
DyLight 405	AF554E
DyLight 488	AF554K
DyLight 550	AF554L
DyLight 594	AF554M
DyLight 650	AF554W
DyLight 680	AF554Y
DyLight 755	AF554Z
FITC	AF554F
HRP	AF554H
Janelia Fluor 525	AF554JF525
Janelia Fluor 549	AF554I
Janelia Fluor 646	AF554J
Janelia Fluor 669	AF554JF669
PE	AF554P
PerCP	AF554C
mFluor Violet 450 SE	AF554MFV450
mFluor Violet 500 SE	AF554MFV500
mFluor Violet 610 SE	AF554MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF554-SP

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NBP3-14741

CD4 Antibody (BLR167J)

100 ul

NBP3-14741

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox">

CD4 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rabbit Anti-Mouse CD4, Monoclonal Antibody (Catalog# NBP3-14741) at 1:250 dilution at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="CD4 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rabbit Anti-Mouse CD4, Monoclonal Antibody (Catalog# NBP3-14741) at 1:250 dilution at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

Western Blot: CD4 Antibody (BLR167J) [NBP3-14741] - Whole cell lysate (10 ug) from NIH 3T3, BW5147.3, mouseThymus, 70Z/3, and EL4 cells prepared using NETN lysisbuffer. Antibody: Rabbit anti-CD4 recombinant monoclonalantibody [BLR167J] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgGChemiluminescence with an exposure timeof 30 seconds. Lower Panel: Rabbit anti-Actin recombinantmonoclonal antibody [BLR057F].

Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741] - Mouse lung, : Rabbit anti-CD4 recombinant monoclonal antibody [BLR167J]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.

Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741] - Mouse spleen, Rabbit anti-CD4 recombinant monoclonal antibody [BLR167J]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741] - Mouse gut, Rabbit anti-CD4 recombinant monoclonal antibody [BLR167J]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunoprecipitation: CD4 Antibody [NBP3-14741] - s: Whole cell lysate (200 ugper IP reaction; 10% of IP loaded) from mouse thymus.Antibodies: Rabbit anti-CD4 recombinant monoclonalantibody used for IP at 6 ul/200ug lysate. CD4 was also immunoprecipitated by anotherantibody against a different epitope of CD4 For blotting immunoprecipitated CD4, was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NB100-2181

CD4 Antibody (B486A1) - BSA Free

0.1 ml

NB100-2181

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-2181AF350
Alexa Fluor 405	NB100-2181AF405
Alexa Fluor 488	NB100-2181AF488
Alexa Fluor 532	NB100-2181AF532
Alexa Fluor 594	NB100-2181AF594
Alexa Fluor 647	NB100-2181AF647
Alexa Fluor 700	NB100-2181AF700
Alexa Fluor 750	NB100-2181AF750
Alexa Fluor Plus 405	NB100-2181AFP405
Alexa Fluor Plus 488	NB100-2181AFP488
Alexa Fluor Plus 555	NB100-2181AFP555
Alexa Fluor Plus 594	NB100-2181AFP594
Alexa Fluor Plus 647	NB100-2181AFP647
Alexa Fluor Plus 680	NB100-2181AFP680
Allophycocyanin	NB100-2181APC
Allophycocyanin/Cy7	NB100-2181APCCY7
Biotin	NB100-2181B
CoraFluor 1	NB100-2181CL1
DyLight 350	NB100-2181UV
DyLight 405	NB100-2181V
DyLight 488	NB100-2181G
DyLight 550	NB100-2181R
DyLight 594	NB100-2181DL594
DyLight 650	NB100-2181C
DyLight 680	NB100-2181FR
DyLight 755	NB100-2181IR
FITC	NB100-2181F
HRP	NB100-2181H
Janelia Fluor 525	NB100-2181JF525
Janelia Fluor 549	NB100-2181JF549
Janelia Fluor 646	NB100-2181JF646
Janelia Fluor 669	NB100-2181JF669
PE	NB100-2181PE
PE/Atto594	NB100-2181PEATT594
PE/Cy5.5	NB100-2181PECY55
PE/Cy7	NB100-2181PECY7
PerCP	NB100-2181PCP
mFluor Violet 450 SE	NB100-2181MFV450
mFluor Violet 500 SE	NB100-2181MFV500
mFluor Violet 610 SE	NB100-2181MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-2181SS
Azide and BSA Free	NBP2-80646

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MAB2410

CD4 Antibody (296712) [Unconjugated]

(1 Review)

(1 Publication)

100 μg

MAB2410

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

CD4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine CD4 Monoclonal Antibody (Catalog # MAB2410) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="CD4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine CD4 Monoclonal Antibody (Catalog # MAB2410) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Reactivity:

Species Glossary

Reactivity: Ca

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2410U-100UG
Alexa Fluor 405	FAB2410V-100UG
Alexa Fluor 488	FAB2410G-100UG
Alexa Fluor 532	FAB2410X
Alexa Fluor 594	FAB2410T-100UG
Alexa Fluor 647	FAB2410R-100UG
Alexa Fluor 700	FAB2410N-100UG
Alexa Fluor 750	FAB2410S-100UG
Alexa Fluor Plus 405	FAB2410AFP405-100UG
Alexa Fluor Plus 488	FAB2410AFP488-100UG
Alexa Fluor Plus 555	FAB2410AFP555-100UG
Alexa Fluor Plus 594	FAB2410AFP594-100UG
Alexa Fluor Plus 647	FAB2410AFP647-100UG
Alexa Fluor Plus 680	FAB2410AFP680-100UG
Allophycocyanin/Cy7	FAB2410APCCY7
Biotin	FAB2410B
CoraFluor 1	FAB2410CL1
DyLight 350	FAB2410D
DyLight 405	FAB2410E
DyLight 488	FAB2410K
DyLight 550	FAB2410L
DyLight 594	FAB2410M
DyLight 650	FAB2410W
DyLight 680	FAB2410Y
DyLight 755	FAB2410Z
FITC	FAB2410F
HRP	FAB2410H
Janelia Fluor 525	FAB2410JF525
Janelia Fluor 549	FAB2410I
Janelia Fluor 646	FAB2410J
Janelia Fluor 669	FAB2410JF669
PE/Atto594	FAB2410PEATT594
PE/Cy5.5	FAB2410PECY55
PE/Cy7	FAB2410PECY7
PerCP	FAB2410C
Phycoerythrin	FAB2410P
mFluor Violet 450 SE	FAB2410MFV450
mFluor Violet 500 SE	FAB2410MFV500
mFluor Violet 610 SE	FAB2410MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB2410P
Carrier Free	MAB2410-SP
Carrier Free	FAB2410V-100UG
Carrier Free	FAB2410U-100UG
Carrier Free	FAB2410T-100UG
Carrier Free	FAB2410S-100UG
Carrier Free	FAB2410R-100UG
Carrier Free	FAB2410N-100UG
Carrier Free	FAB2410G-100UG

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NBP3-18057

CD4 Antibody - BSA Free

0.1 mg

NBP3-18057

Flow Cytometry: CD4 Antibody [NBP3-18057] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP3-18057 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunohistochemistry-Paraffin: Rabbit CD4 pAb [NBP3-18057] - Immunofluorescence staining of human tonsil FFPE tissue. Antibody diluted 1:50 (Catalog # NBP3-18057AF647) in 3% BSA and incubated over night at 4 °C. Heat mediated antigen retrieval at pH 9. Image from a verified customer review.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-18057AF350
Alexa Fluor 405	NBP3-18057AF405
Alexa Fluor 488	NBP3-18057AF488
Alexa Fluor 532	NBP3-18057AF532
Alexa Fluor 594	NBP3-18057AF594
Alexa Fluor 647	NBP3-18057AF647
Alexa Fluor 700	NBP3-18057AF700
Alexa Fluor 750	NBP3-18057AF750
Alexa Fluor Plus 405	NBP3-18057AFP405
Alexa Fluor Plus 488	NBP3-18057AFP488
Alexa Fluor Plus 555	NBP3-18057AFP555
Alexa Fluor Plus 594	NBP3-18057AFP594
Alexa Fluor Plus 647	NBP3-18057AFP647
Alexa Fluor Plus 680	NBP3-18057AFP680
Allophycocyanin	NBP3-18057APC
Biotin	NBP3-18057B
CoraFluor 1	NBP3-18057CL1
DyLight 350	NBP3-18057UV
DyLight 405	NBP3-18057V
DyLight 488	NBP3-18057G
DyLight 550	NBP3-18057R
DyLight 594	NBP3-18057DL594
DyLight 650	NBP3-18057C
DyLight 680	NBP3-18057FR
DyLight 755	NBP3-18057IR
FITC	NBP3-18057F
HRP	NBP3-18057H
Janelia Fluor 525	NBP3-18057JF525
Janelia Fluor 549	NBP3-18057JF549
Janelia Fluor 646	NBP3-18057JF646
Janelia Fluor 669	NBP3-18057JF669
PE	NBP3-18057PE
PerCP	NBP3-18057PCP
mFluor Violet 450 SE	NBP3-18057MFV450
mFluor Violet 500 SE	NBP3-18057MFV500
mFluor Violet 610 SE	NBP3-18057MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-18057SS

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

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Research Areas

Has Sample Size

Promotional Item

Species Glossary

CD4 Antibodies

CD4 Antibody - BSA Free

CD4 Antibody [Unconjugated]

CD4 Antibody (RPA-T4) - Azide and BSA Free

CD4 Antibody (GK1.5) - BSA Free

CD4 Antibody (W3/25) - BSA Free

CD4 Antibody (34930) [Unconjugated]

CD4 Antibody (BL-155-1C11) - BSA Free

CD4 Antibody [Unconjugated]

CD4 Antibody (BLR167J)

CD4 Antibody (B486A1) - BSA Free

CD4 Antibody (296712) [Unconjugated]

CD4 Antibody - BSA Free