Mouse Kidney Whole Tissue Lysate (Adult Whole Normal)

Images

 
Western Blot: Mouse Kidney Whole Tissue Lysate (Adult Normal) [NB820-59661] - Western blot analysis of Actin (NB600-532) using NB820-59961.
SDS-Page: Mouse Kidney Whole Tissue Lysate (Adult Normal) [NB820-59661] - Ponceau S staining of whole normal mouse kidney lysate using NB820-59661.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

Mouse Kidney Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Mouse Normal Tissue: Kidney

Applications/Dilutions

Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Reviewed Applications
Read 1 Review rated 5
using
NB820-59661 in the following application:

Reactivity Notes

Mouse

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Tissue
Life Stage
Adult
Tissue
Digestive/Waste
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Notes

Protein supplied in 1x Laemmli Sample Buffer without 2-mercaptoethanol. 2-Mercaptoethanol should be added to 5% prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Review for Kidney Lysate (NB820-59661) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB820-59661:
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WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Benjamin Ko
WB Mouse 07/21/2010
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Summary

ApplicationWestern Blot
Sample TestedMouse kidney whole cell lysate, Sample Amount: 80ug
SpeciesMouse
Lotb307253
CommentsExcellent whole kidney lysate to serve as positive control for Western blotting.

Blocking

Blocking DetailsBlocking Buffer: 3% BSA in TBST, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: TBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Alexa, Secondary Ab Dilution Ratio: 1:10000

Details

Detection NotesDetection Method: Licor, Positive Control: none

Comments

CommentsExcellent whole kidney lysate to serve as positive control for Western blotting.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

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FAQs for Kidney Lysate (NB820-59661) (0)

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Calculators

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Recent Reviews

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Benjamin Ko
07/21/2010
Application: WB
Species: Mouse