Mouse Kidney Whole Tissue Lysate (Adult Whole Normal) Summary
Total Protein - Mouse Normal Tissue: Kidney
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Lysate Details for Array
Protein supplied in 1x Laemmli Sample Buffer without 2-mercaptoethanol. 2-Mercaptoethanol should be added to 5% prior to use.
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
for 6 months from date of receipt.
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Kidney Lysate (NB820-59661) (1)
Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
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Benjamin Ko WB
Application Western Blot Sample Tested Mouse kidney whole cell lysate, Sample Amount: 80ug Species Mouse Lot b307253 Comments Excellent whole kidney lysate to serve as positive control for Western blotting. Blocking
Blocking Details Blocking Buffer: 3% BSA in TBST, Blocking Time: 2 hours, Blocking Temp: Room temperature Primary Anitbody
Dilution Ratio Primary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: TBST, Incubation Time: overnight, Incubation Temp: 4C Secondary Antibody
Secondary Description Secondary Ab: Alexa, Secondary Ab Dilution Ratio: 1:10000 Details
Detection Notes Detection Method: Licor, Positive Control: none Comments
Comments Excellent whole kidney lysate to serve as positive control for Western blotting.
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