CCR5 Antibody (45531) [Unconjugated]

Images

 
Human peripheral blood mononuclear cells (PBMCs) were stained with either (A) Mouse Anti-Human CCR5 Monoclonal Antibody (Catalog # MAB182) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by Goat ...read more
CCR5 was detected in immersion fixed human dendritic cells using Human CCR5 Monoclonal Antibody (Catalog # MAB182) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ ...read more
Recombinant Human CCL3/MIP‑1 alpha (Catalog # 270-LD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready, ICC/IF
Clone
45531
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

CCR5 Antibody (45531) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human CCR5
Met1-Leu352
Accession # P51681
Specificity
Detects human CCR5.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CCR5
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 5-20 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB182 in the following applications:

Publications
Read Publications using
MAB182 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR5 Antibody (45531) [Unconjugated]

  • C-C CKR-5
  • C-C motif chemokine receptor 5 A159A
  • CCCKR5
  • CC-CKR-5FLJ78003
  • CCR5
  • CCR-5
  • CD195 antigen
  • CD195
  • chemokine (C-C motif) receptor 5
  • chemokine receptor CCR5
  • chemr13
  • CKR5
  • CKR-5
  • HIV-1 fusion coreceptor
  • IDDM22CMKBR5C-C chemokine receptor type 5

Background

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha , MIP-1 beta , MCP-2, and RANTES. It also functions as a coreceptor for macrophage tropic HIV-1 infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR5 Antibody (MAB182)(30)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: Bioassay, Competitive Binding Assay, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Neutralization.


Filter By Application
Bioassay
(1)
Competitive Binding Assay
(1)
Flow Cytometry
(13)
ICC
(5)
IHC
(1)
IHC-Fr
(2)
IHC-P
(3)
Neutralization
(10)
All Applications
Filter By Species
Human
(28)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using MAB182 Applications Species
Lee, JW;Lee, IH;Watanabe, H;Liu, Y;Sawada, K;Maekawa, M;Uehara, S;Kobayashi, Y;Imai, Y;Kong, SW;Iimura, T; Centrosome clustering control in osteoclasts through CCR5-mediated signaling Scientific reports 2023-11-27 [PMID: 38012303] (Neutralization, Human) Neutralization Human
M Weichseldo, Y Tagaya, M Reitz, AL DeVico, OS Latinovic Identifying CCR5 coreceptor populations permissive for HIV-1 entry and productive infection: implications for in vivo studies Journal of Translational Medicine, 2022-01-24;20(1):39. 2022-01-24 [PMID: 35073923] (ICC, Human) ICC Human
MC Mladinich, JN Conde, WR Schutt, SY Sohn, ER Mackow Blockade of Autocrine CCL5 Responses Inhibits Zika Virus Persistence and Spread in Human Brain Microvascular Endothelial Cells MBio, 2021-08-17;12(4):e0196221. 2021-08-17 [PMID: 34399621] (Neutralization, Human) Neutralization Human
C Jacquemard, F Koensgen, P Colin, B Lagane, E Kellenberg Modeling of CCR5 Recognition by HIV-1 gp120: How the Viral Protein Exploits the Conformational Plasticity of the Coreceptor Viruses, 2021-07-18;13(7):. 2021-07-18 [PMID: 34372601] (Flow Cytometry, Human) Flow Cytometry Human
YF Sun, L Wu, SP Liu, MM Jiang, B Hu, KQ Zhou, W Guo, Y Xu, Y Zhong, XR Zhou, ZF Zhang, G Liu, S Liu, YH Shi, Y Ji, M Du, NN Li, GB Li, ZK Zhao, XY Huang, LQ Xu, QC Yu, DH Peng, SJ Qiu, HC Sun, M Dean, XD Wang, WY Chung, AR Dennison, J Zhou, Y Hou, J Fan, XR Yang Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma Nature Communications, 2021-07-02;12(1):4091. 2021-07-02 [PMID: 34215748] (IHC, Human) IHC Human
G Nishikawa, K Kawada, J Nakagawa, K Toda, R Ogawa, S Inamoto, R Mizuno, Y Itatani, Y Sakai Bone marrow-derived mesenchymal stem cells promote colorectal cancer progression via CCR5 Cell Death Dis, 2019-03-19;10(4):264. 2019-03-19 [PMID: 30890699] (Flow Cytometry, Human) Flow Cytometry Human
P Colin, Z Zhou, I Staropoli, J Garcia-Per, R Gasser, M Armani-Tou, Y Benureau, N Gonzalez, J Jin, BJ Connell, S Raymond, P Delobel, J Izopet, H Lortat-Jac, J Alcami, F Arenzana-S, A Brelot, B Lagane CCR5 structural plasticity shapes HIV-1 phenotypic properties PLoS Pathog., 2018-12-06;14(12):e1007432. 2018-12-06 [PMID: 30521629] (Competitive Binding Assay, Human) Competitive Binding Assay Human
JW Lee, A Hoshino, K Inoue, T Saitou, S Uehara, Y Kobayashi, S Ueha, K Matsushima, A Yamaguchi, Y Imai, T Iimura The HIV co-receptor CCR5 regulates osteoclast function Nat Commun, 2017-12-20;8(1):2226. 2017-12-20 [PMID: 29263385] (Neutralization, Mouse) Neutralization Mouse
WL Wu, CR Grotefend, MT Tsai, YL Wang, V Radic, H Eoh, IC Huang ?20 IFITM2 differentially restricts X4 and R5 HIV-1 Proc. Natl. Acad. Sci. U.S.A., 2017-06-19;114(27):7112-7117. 2017-06-19 [PMID: 28630320] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
James M. Fox, Richard Kasprowicz, Oliver Hartley, Nathalie Signoret CCR5 susceptibility to ligand-mediated down-modulation differs between human T lymphocytes and myeloid cells Journal of Leukocyte Biology 2015-07-01 [PMID: 25957306]
Show All 30 Publications.

Review for CCR5 Antibody (MAB182) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB182:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry CCR5 MAB182
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4
reviewed by:
Verified Customer
Flow Human 08/28/2018
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Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood mononuclear cells (PBMCs)
SpeciesHuman
LotAWK0717121

Comments

CommentsFreshly isolated human PBMCs were stained with mouse anti-human CCR5 monoclonal antibody (MAB182) or mIgG2b isotype control at 2.5 µg/ml for 30 minutes on ice followed by goat anti-mouse IgG PE secondary staining. Additional CD3 APC staining.

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Verified Customer
08/28/2018
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CCR5
Uniprot