CCR5 Antibody (45531) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) were stained with either (A) Mouse Anti-Human CCR5 Monoclonal Antibody (Catalog # MAB182) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.

Recombinant Human CCL3/MIP‑1 alpha (Catalog # 270-LD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL3/MIP‑1 alpha (40 ng/mL) is neutralized (green line) by increasing concentrations of Human CCR5 Monoclonal Antibody (Catalog # MAB182). The ND50 is typically 5-20 µg/mL.

CCR5 was detected in immersion fixed human dendritic cells using Human CCR5 Monoclonal Antibody (Catalog # MAB182) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: Flow, B/N, CyTOF-ready, ICC/IF
• Clone: 45531
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR5 Antibody (45531) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CCR5
  Met1-Leu352
  Accession # P51681
• Specificity: Detects human CCR5.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR5
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 5-20 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR5 Antibody (45531) [Unconjugated]

• C-C CKR-5
• C-C motif chemokine receptor 5 A159A
• CCCKR5
• CC-CKR-5FLJ78003
• CCR5
• CCR-5
• CD195 antigen
• CD195
• chemokine (C-C motif) receptor 5
• chemokine receptor CCR5
• chemr13
• CKR5
• CKR-5
• HIV-1 fusion coreceptor
• IDDM22CMKBR5C-C chemokine receptor type 5

Background

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha , MIP-1 beta , MCP-2, and RANTES. It also functions as a coreceptor for macrophage tropic HIV-1 infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR5 Antibody (MAB182)(25)

Publications using MAB182

• YF Sun, L Wu, SP Liu, MM Jiang, B Hu, KQ Zhou, W Guo, Y Xu, Y Zhong, XR Zhou, ZF Zhang, G Liu, S Liu, YH Shi, Y Ji, M Du, NN Li, GB Li, ZK Zhao, XY Huang, LQ Xu, QC Yu, DH Peng, SJ Qiu, HC Sun, M Dean, XD Wang, WY Chung, AR Dennison, J Zhou, Y Hou, J Fan, XR Yang Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma Nature Communications, 2021;12(1):4091. 2021 [PMID: 34215748] (IHC, Human)
• G Nishikawa, K Kawada, J Nakagawa, K Toda, R Ogawa, S Inamoto, R Mizuno, Y Itatani, Y Sakai Bone marrow-derived mesenchymal stem cells promote colorectal cancer progression via CCR5 Cell Death Dis, 2019;10(4):264. 2019 [PMID: 30890699] (Flow Cytometry, Human)
• P Colin, Z Zhou, I Staropoli, J Garcia-Per, R Gasser, M Armani-Tou, Y Benureau, N Gonzalez, J Jin, BJ Connell, S Raymond, P Delobel, J Izopet, H Lortat-Jac, J Alcami, F Arenzana-S, A Brelot, B Lagane CCR5 structural plasticity shapes HIV-1 phenotypic properties PLoS Pathog., 2018;14(12):e1007432. 2018 [PMID: 30521629] (Competitive Binding Assay, Human)
• JW Lee, A Hoshino, K Inoue, T Saitou, S Uehara, Y Kobayashi, S Ueha, K Matsushima, A Yamaguchi, Y Imai, T Iimura The HIV co-receptor CCR5 regulates osteoclast function Nat Commun, 2017;8(1):2226. 2017 [PMID: 29263385] (Neutralization, Mouse)
• WL Wu, CR Grotefend, MT Tsai, YL Wang, V Radic, H Eoh, IC Huang ?20 IFITM2 differentially restricts X4 and R5 HIV-1 Proc. Natl. Acad. Sci. U.S.A., 2017;114(27):7112-7117. 2017 [PMID: 28630320] (Flow Cytometry, Human)
• Ward , S T, Li , K K, Hepburn , E, Weston , C J, Curbishley , S M, Reynolds , G M, Hejmadi , R K, Bicknell , R, Eksteen , B, Ismail , T, Rot , A, Adams , D H The effects of CCR5 inhibition on regulatory T-cell recruitment to colorectal cancer. Br J Cancer, 2015;112(2):319-28. 2015 [PMID: 25405854] (IHC-P, Human)
• Hartmann N, Giese N, Giese T, Poschke I, Offringa R, Werner J, Ryschich E Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer. Clin Cancer Res, 2014;20(13):3422-33. 2014 [PMID: 24763614] (Flow Cytometry, Human)
• Nakano Y, Monde K, Terasawa H, Yuan Y, Yusa K, Harada S, Maeda Y Preferential recognition of monomeric CCR5 expressed in cultured cells by the HIV-1 envelope glycoprotein gp120 for the entry of R5 HIV-1. Virology, 0;452(0):117-24. 0 [PMID: 24606688] (Flow Cytometry, Human)
• Flegler A, Cianci G, Hope T CCR5 conformations are dynamic and modulated by localization, trafficking and G protein association. PLoS ONE, 2014;9(2):e89056. 2014 [PMID: 24586501] (IHC-Fr, Human)
• Alonzo , Francis, Kozhaya , Lina, Rawlings , Stephen, Reyes-Robles , Tamara, DuMont , Ashley L, Myszka , David G, Landau , Nathanie, Unutmaz , Derya, Torres , Victor J CCR5 is a receptor for Staphylococcus aureus leukotoxin ED. Nature, 2013;493(7430):51-5. 2013 [PMID: 23235831] (Bioassay, Human)
• Duluc , D, Gannevat , J, Anguiano , E, Zurawski , S, Carley , M, Boreham , M, Stecher , J, Dullaers , M, Banchereau , J, Oh , S Functional diversity of human vaginal APC subsets in directing T-cell responses. Mucosal Immunol, 2013;6(3):626-38. 2013 [PMID: 23131784] (Flow Cytometry, Human)
• Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Int. J. Cancer, 2010;127(2):332-44. 2010 [PMID: 19937793] (Neutralization, Human)
• Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention. Cancer Res., 2009;69(15):6249-55. 2009 [PMID: 19638592] (Flow Cytometry, Human)
• Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008;181(11):8053-67. 2008 [PMID: 19017998] (Flow Cytometry, Human)
• Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions. Int. J. Cancer, 2008;122(4):769-76. 2008 [PMID: 17935139] (Flow Cytometry, Human)
• Bolovan-Fritts CA, Spector SA Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction. Blood, 2007;111(1):175-82. 2007 [PMID: 17895402] (Neutralization, Human)
• Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. J. Immunol., 2007;179(6):3724-33. 2007 [PMID: 17785809] (Flow Cytometry, Human)
• Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells. Cytokine, 2006;36(5):237-44. 2006 [PMID: 17350279] (Neutralization, Human)
• Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006;120(2):220-31. 2006 [PMID: 16782408] (Neutralization, Human)
• Lepej SZ, Rode OD, Jeren T, Vince A, Remenar A, Barsic B Increased expression of CXCR3 and CCR5 on memory CD4+ T-cells migrating into the cerebrospinal fluid of patients with neuroborreliosis: the role of CXCL10 and CXCL11. J. Neuroimmunol., 2005;163(1):128-34. 2005 [PMID: 15885315] (Flow Cytometry, Human)
• Lim HW, Hillsamer P, Kim CH Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses. J. Clin. Invest., 2004;114(11):1640-9. 2004 [PMID: 15578096] (ICC, Human)
• Ottonello L, Montecucco F, Bertolotto M, Arduino N, Mancini M, Corcione A, Pistoia V, Dallegri F CCL3 (MIP-1alpha) induces in vitro migration of GM-CSF-primed human neutrophils via CCR5-dependent activation of ERK 1/2. Cell. Signal., 2005;17(3):355-63. 2005 [PMID: 15567066] (Flow Cytometry, Human)
• Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. J. Leukoc. Biol., 2003;73(5):584-90. 2003 [PMID: 12714572] (Neutralization, Human)
• Howard OM, Dong HF, Yang D, Raben N, Nagaraju K, Rosen A, Casciola-Rosen L, Hartlein M, Kron M, Yiadom K, Dwivedi S, Plotz PH, Oppenheim JJ Histidyl-tRNA synthetase and asparaginyl-tRNA synthetase, autoantigens in myositis, activate chemokine receptors on T lymphocytes and immature dendritic cells. J. Exp. Med., 2002;196(6):781-91. 2002 [PMID: 12235211] (Neutralization, Human)
• Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function. J. Biol. Chem., 1999;274(14):9617-26. 1999 [PMID: 10092648] (Flow Cytometry, Human)

Review for CCR5 Antibody (MAB182) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 08/28/2018

Summary

• Application: Flow Cytometry
• Sample Tested: Peripheral blood mononuclear cells (PBMCs)
• Species: Human
• Lot: AWK0717121

Comments

• Comments: Freshly isolated human PBMCs were stained with mouse anti-human CCR5 monoclonal antibody &#40;MAB182&#41; or mIgG2b isotype control at 2.5 µg/ml for 30 minutes on ice followed by goat anti-mouse IgG PE secondary staining. Additional CD3 APC staining.

Bioinformatics

• Gene Symbol: CCR5
• Uniprot:
  • P51681()