CCR5 Antibody (45531) [Unconjugated]

Images

 
Human peripheral blood mononuclear cells (PBMCs) were stained with either (A) Mouse Anti-Human CCR5 Monoclonal Antibody (Catalog # MAB182) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by Goat ...read more
Recombinant Human CCL3/MIP‑1 alpha (Catalog # 270-LD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that ...read more
CCR5 was detected in immersion fixed human dendritic cells using Human CCR5 Monoclonal Antibody (Catalog # MAB182) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready, ICC/IF
Clone
45531
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

CCR5 Antibody (45531) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human CCR5
Met1-Leu352
Accession # P51681
Specificity
Detects human CCR5.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CCR5
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 5-20 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB182 in the following applications:

Publications
Read Publications using
MAB182 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR5 Antibody (45531) [Unconjugated]

  • C-C CKR-5
  • C-C motif chemokine receptor 5 A159A
  • CCCKR5
  • CC-CKR-5FLJ78003
  • CCR5
  • CCR-5
  • CD195 antigen
  • CD195
  • chemokine (C-C motif) receptor 5
  • chemokine receptor CCR5
  • chemr13
  • CKR5
  • CKR-5
  • HIV-1 fusion coreceptor
  • IDDM22CMKBR5C-C chemokine receptor type 5

Background

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha , MIP-1 beta , MCP-2, and RANTES. It also functions as a coreceptor for macrophage tropic HIV-1 infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCR5 Antibody (MAB182)(25)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: Bioassay, Competitive Binding Assay, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Neutralization.


Filter By Application
Bioassay
(1)
Competitive Binding Assay
(1)
Flow Cytometry
(12)
ICC
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
Neutralization
(7)
All Applications
Filter By Species
Human
(24)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using MAB182 Applications Species
YF Sun, L Wu, SP Liu, MM Jiang, B Hu, KQ Zhou, W Guo, Y Xu, Y Zhong, XR Zhou, ZF Zhang, G Liu, S Liu, YH Shi, Y Ji, M Du, NN Li, GB Li, ZK Zhao, XY Huang, LQ Xu, QC Yu, DH Peng, SJ Qiu, HC Sun, M Dean, XD Wang, WY Chung, AR Dennison, J Zhou, Y Hou, J Fan, XR Yang Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma Nature Communications, 2021;12(1):4091. 2021 [PMID: 34215748] (IHC, Human) IHC Human
G Nishikawa, K Kawada, J Nakagawa, K Toda, R Ogawa, S Inamoto, R Mizuno, Y Itatani, Y Sakai Bone marrow-derived mesenchymal stem cells promote colorectal cancer progression via CCR5 Cell Death Dis, 2019;10(4):264. 2019 [PMID: 30890699] (Flow Cytometry, Human) Flow Cytometry Human
P Colin, Z Zhou, I Staropoli, J Garcia-Per, R Gasser, M Armani-Tou, Y Benureau, N Gonzalez, J Jin, BJ Connell, S Raymond, P Delobel, J Izopet, H Lortat-Jac, J Alcami, F Arenzana-S, A Brelot, B Lagane CCR5 structural plasticity shapes HIV-1 phenotypic properties PLoS Pathog., 2018;14(12):e1007432. 2018 [PMID: 30521629] (Competitive Binding Assay, Human) Competitive Binding Assay Human
JW Lee, A Hoshino, K Inoue, T Saitou, S Uehara, Y Kobayashi, S Ueha, K Matsushima, A Yamaguchi, Y Imai, T Iimura The HIV co-receptor CCR5 regulates osteoclast function Nat Commun, 2017;8(1):2226. 2017 [PMID: 29263385] (Neutralization, Mouse) Neutralization Mouse
WL Wu, CR Grotefend, MT Tsai, YL Wang, V Radic, H Eoh, IC Huang ?20 IFITM2 differentially restricts X4 and R5 HIV-1 Proc. Natl. Acad. Sci. U.S.A., 2017;114(27):7112-7117. 2017 [PMID: 28630320] (Flow Cytometry, Human) Flow Cytometry Human
Ward , S T, Li , K K, Hepburn , E, Weston , C J, Curbishley , S M, Reynolds , G M, Hejmadi , R K, Bicknell , R, Eksteen , B, Ismail , T, Rot , A, Adams , D H The effects of CCR5 inhibition on regulatory T-cell recruitment to colorectal cancer. Br J Cancer, 2015;112(2):319-28. 2015 [PMID: 25405854] (IHC-P, Human) IHC-P Human
Hartmann N, Giese N, Giese T, Poschke I, Offringa R, Werner J, Ryschich E Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer. Clin Cancer Res, 2014;20(13):3422-33. 2014 [PMID: 24763614] (Flow Cytometry, Human) Flow Cytometry Human
Nakano Y, Monde K, Terasawa H, Yuan Y, Yusa K, Harada S, Maeda Y Preferential recognition of monomeric CCR5 expressed in cultured cells by the HIV-1 envelope glycoprotein gp120 for the entry of R5 HIV-1. Virology, 0;452(0):117-24. 0 [PMID: 24606688] (Flow Cytometry, Human) Flow Cytometry Human
Flegler A, Cianci G, Hope T CCR5 conformations are dynamic and modulated by localization, trafficking and G protein association. PLoS ONE, 2014;9(2):e89056. 2014 [PMID: 24586501] (IHC-Fr, Human) IHC-Fr Human
Alonzo , Francis, Kozhaya , Lina, Rawlings , Stephen, Reyes-Robles , Tamara, DuMont , Ashley L, Myszka , David G, Landau , Nathanie, Unutmaz , Derya, Torres , Victor J CCR5 is a receptor for Staphylococcus aureus leukotoxin ED. Nature, 2013;493(7430):51-5. 2013 [PMID: 23235831] (Bioassay, Human) Bioassay Human
Show All 25 Publications.

Review for CCR5 Antibody (MAB182) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB182:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry CCR5 MAB182
Enlarge
4
reviewed by:
Verified Customer
Flow Human 08/28/2018
View

Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood mononuclear cells (PBMCs)
SpeciesHuman
LotAWK0717121

Comments

CommentsFreshly isolated human PBMCs were stained with mouse anti-human CCR5 monoclonal antibody &#40;MAB182&#41; or mIgG2b isotype control at 2.5 µg/ml for 30 minutes on ice followed by goat anti-mouse IgG PE secondary staining. Additional CD3 APC staining.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CCR5 Antibody (MAB182) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB182U
Alexa Fluor 405 FAB182V
Alexa Fluor 488 FAB182G
Alexa Fluor 532 FAB182X
Alexa Fluor 594 FAB182T
Alexa Fluor 647 FAB182R
Alexa Fluor 700 FAB182N
Alexa Fluor 750 FAB182S
Allophycocyanin FAB182A
DyLight 350 FAB182D
DyLight 405 FAB182E
DyLight 488 FAB182K
DyLight 550 FAB182L
DyLight 594 FAB182M
DyLight 650 FAB182W
DyLight 680 FAB182Y
DyLight 755 FAB182Z
Fluorescein FAB182F
Fluorescein FAB182F
Janelia Fluor 549 FAB182I
Janelia Fluor 646 FAB182J
PerCP FAB182C
Phycoerythrin FAB182P
Phycoerythrin FAB182P

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Recent Reviews

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5
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1
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Verified Customer
08/28/2018
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CCR5
Uniprot