MHC Class I Antibody (OX18)


Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, more
Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved more
Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).
Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The more
Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells more
Flow (Intracellular): MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were stained with MHC Class I NB120-6405 (Blue) and a matched isotype control NBP2-27287 (Orange). Cells were fixed with 4% PFA and then more

Product Details

Reactivity Mu, RtSpecies Glossary
Applications ELISA, EM, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, CyTOF-ready
1.0 mg/ml

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MHC Class I Antibody (OX18) Summary

Rat spleen glycoproteins
Cell Membrane; Type I membrane protein
Recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognised.
Protein G purified
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  • Electron Microscopy
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Block/Neutralize
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
The epitope recognized by this anti-rat RT1-A antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 24678820). ELISA reported in scientific literature (PMID: 2783579). Use in Electron Microscopy reported in scientific literature (PMID: 3044648). Use in blocking/neutralizing reported in scientific literature (PMID: 1698855). This antibody is CyTOF ready.
Read Publications using
NB120-6405 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32580156).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for MHC Class I Antibody (OX18)

  • Clone OX18
  • DKFZp686P19218
  • EA1.2
  • EA2.1
  • HLA class I histocompatibility antigen, alpha chain E
  • HLA class I histocompatibility antigen, E alpha chain
  • HLA-6.2
  • HLAE
  • lymphocyte antigen
  • major histocompatibility complex, class I, E
  • MHC class I antigen E
  • MHC Class I RT1-A
  • MHC HLA-E alpha-1
  • MHC HLA-E alpha-2.1
  • MHC
  • OX18
  • QA1
  • RT1-A (OX18)
  • RT1-A


MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MHC Class I Antibody (NB120-6405)(32)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 5 applications: B/N, FLOW, ICC/IF, IHC-Fr, IP.

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Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using NB120-6405 Applications Species
Smith TA, Ghergherehchi CL, Mikesh M et al. Polyethylene glycol-fusion repair of sciatic allografts in female rats achieves immunotolerance via attenuated innate and adaptive responses J. Neurosci. Res. Sep 15 2020 [PMID: 32931034]
Stet, RJM et al. U9F4: A monoclonal antibody recognizing a rat polymorphic class I determinant. 1987
Rabenstein M, Unverricht-Yeboah M, Keuters MH et al. Transcranial Current Stimulation Alters the Expression of Immune-Mediating Genes Front Cell Neurosci. Oct 25 2019 [PMID: 31708742] (IHC-Fr, Rat) IHC-Fr Rat
Stettner M, Lohmann B, Wolffram K et al. Interleukin-17 impedes Schwann cell-mediated myelination. J Neuroinflammation 3/29/2014 [PMID: 24678820] (ICC/IF, Rat) ICC/IF Rat
Bereczky-Veress B, Abdelmagid N, Piehl F et al. Influence of perineurial cells and Toll-like receptors 2 and 9 on Herpes simplex type 1 entry to the central nervous system in rat encephalitis. PLoS One. 2010 Aug 27 [PMID: 20806060] (IHC-Fr, Rat) IHC-Fr Rat
Baca Jones, CC et al. Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells. 2009 [PMID: 19349057]
Zhai, Y Knechtle, S et al. Two distinct forms of soluble MHC class I molecules synthesized by different mechanisms in normal rat cells in vitro. 1998 [PMID: 9684990]
Morrice NA, Powis SJ. A role for the thiol-dependent reductase ERp57 in the assembly of MHC class I molecules. Curr Biol. 1998 Jun 4 [PMID: 9637924] (IP, Rat) IP Rat
Lassmann H, Schmied M, Vass K, Hickey WF. Bone marrow derived elements and resident microglia in brain inflammation. Glia. 1993 Jan 1 [PMID: 7678581]
Show All 32 Publications.

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Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for MHC Class I Antibody (NB120-6405) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB120-6405AF350
Alexa Fluor 405 NB120-6405AF405
Alexa Fluor 488 NB120-6405AF488
Alexa Fluor 532 NB120-6405AF532
Alexa Fluor 594 NB120-6405AF594
Alexa Fluor 647 NB120-6405AF647
Alexa Fluor 700 NB120-6405AF700
Alexa Fluor 750 NB120-6405AF750
Allophycocyanin NB120-6405APC
Allophycocyanin/Cy7 NB120-6405APCCY7
Biotin NB120-6405B
DyLight 350 NB120-6405UV
DyLight 405 NB120-6405V
DyLight 488 NB120-6405G
DyLight 550 NB120-6405R
DyLight 594 NB120-6405DL594
DyLight 650 NB120-6405C
DyLight 680 NB120-6405FR
DyLight 755 NB120-6405IR
FITC NB120-6405F
HRP NB120-6405H
Janelia Fluor 549 NB120-6405JF549
Janelia Fluor 646 NB120-6405JF646
PE NB120-6405PE
PE/Atto594 NB120-6405PEATT594
PE/Cy5.5 NB120-6405PECY55
PE/Cy7 NB120-6405PECY7
PerCP NB120-6405PCP

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The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

Major Histocompatibility Complex (MHC) Class I
The products of MHC genes are antigen-presenting molecules (APMs) designed for antigen fragment (peptide) presentation to the T-cell receptor. In particular, MHC Class I molecules play a key role in the immune system by presenting endogenously synthes...  Read full blog post.

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Gene Symbol HLA-E