MHC Class I Antibody (OX18)

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Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells ...read more
Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved ...read more
Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).
Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The ...read more
Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, ...read more
Flow (Intracellular): MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were stained with MHC Class I NB120-6405 (Blue) and a matched isotype control NBP2-27287 (Orange). Cells were fixed with 4% PFA and then ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA, EM, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, CyTOF-ready
Clone
OX18
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

MHC Class I Antibody (OX18) Summary

Immunogen
Rat spleen glycoproteins
Localization
Cell Membrane; Type I membrane protein
Specificity
Recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognised.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HLA-E
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Electron Microscopy
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Block/Neutralize
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
The epitope recognized by this anti-rat RT1-A antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 24678820). ELISA reported in scientific literature (PMID: 2783579). Use in Electron Microscopy reported in scientific literature (PMID: 3044648). Use in blocking/neutralizing reported in scientific literature (PMID: 1698855). This antibody is CyTOF ready.
Publications
Read Publications using
NB120-6405 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for MHC Class I Antibody (OX18)

  • Clone OX18
  • DKFZp686P19218
  • EA1.2
  • EA2.1
  • HLA class I histocompatibility antigen, alpha chain E
  • HLA class I histocompatibility antigen, E alpha chain
  • HLA-6.2
  • HLAE
  • lymphocyte antigen
  • major histocompatibility complex, class I, E
  • MHC class I antigen E
  • MHC Class I RT1-A
  • MHC HLA-E alpha-1
  • MHC HLA-E alpha-2.1
  • MHC
  • OX18
  • QA1
  • RT1-A (OX18)
  • RT1-A

Background

MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MHC Class I Antibody (NB120-6405)(31)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 5 applications: B/N, FLOW, ICC/IF, IHC-Fr, IP.


Filter By Application
B/N
(1)
FLOW
(2)
ICC/IF
(2)
IHC-Fr
(6)
IP
(2)
All Applications
Filter By Species
Rat
(11)
All Species
Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using NB120-6405 Applications Species
Rabenstein M, Unverricht-Yeboah M, Keuters MH et al. Transcranial Current Stimulation Alters the Expression of Immune-Mediating Genes Front Cell Neurosci. Oct 25 2019 [PMID: 31708742] (IHC-Fr, Rat) IHC-Fr Rat
Stettner M, Lohmann B, Wolffram K et al. Interleukin-17 impedes Schwann cell-mediated myelination. J Neuroinflammation 3/29/2014 [PMID: 24678820] (ICC/IF, Rat) ICC/IF Rat
Bereczky-Veress B, Abdelmagid N, Piehl F et al. Influence of perineurial cells and Toll-like receptors 2 and 9 on Herpes simplex type 1 entry to the central nervous system in rat encephalitis. PLoS One. 2010 Aug 27 [PMID: 20806060] (IHC-Fr, Rat) IHC-Fr Rat
Baca Jones, CC et al. Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells. 2009 [PMID: 19349057]
Zhai, Y Knechtle, S et al. Two distinct forms of soluble MHC class I molecules synthesized by different mechanisms in normal rat cells in vitro. 1998 [PMID: 9684990]
Morrice NA, Powis SJ. A role for the thiol-dependent reductase ERp57 in the assembly of MHC class I molecules. Curr Biol. 1998 Jun 4 [PMID: 9637924] (IP, Rat) IP Rat
Lassmann H, Schmied M, Vass K, Hickey WF. Bone marrow derived elements and resident microglia in brain inflammation. Glia. 1993 Jan 1 [PMID: 7678581]
Mayrhofer G, Schon-Hegrad MA. Ia antigens in rat kidney, with special reference to their expression in tubular epithelium. J Exp Med. 1983 Jun 1 [PMID: 6406641] (IHC-Fr, Rat) IHC-Fr Rat
Mayrhofer G, Pugh CW, Barclay AN. The distribution, ontogeny and origin in the rat of Ia-positive cells with dendritic morphology and of Ia antigen in epithelia, with special reference to the intestine. Eur J Immunol. 1983 Feb 1 [PMID: 6403355]
Fukumoto, T et al. Mouse monoclonal antibodies against rat major histocompatibility antigens. 1982 [PMID: 6178598]
Show All 31 Publications.

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Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for MHC Class I Antibody (NB120-6405) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB120-6405AF350
Alexa Fluor 405 NB120-6405AF405
Alexa Fluor 488 NB120-6405AF488
Alexa Fluor 532 NB120-6405AF532
Alexa Fluor 594 NB120-6405AF594
Alexa Fluor 647 NB120-6405AF647
Alexa Fluor 700 NB120-6405AF700
Alexa Fluor 750 NB120-6405AF750
Allophycocyanin NB120-6405APC
Allophycocyanin/Cy7 NB120-6405APCCY7
Biotin NB120-6405B
DyLight 350 NB120-6405UV
DyLight 405 NB120-6405V
DyLight 488 NB120-6405G
DyLight 550 NB120-6405R
DyLight 594 NB120-6405DL594
DyLight 650 NB120-6405C
DyLight 680 NB120-6405FR
DyLight 755 NB120-6405IR
FITC NB120-6405F
HRP NB120-6405H
Janelia Fluor 549 NB120-6405JF549
Janelia Fluor 646 NB120-6405JF646
PE NB120-6405PE
PE/Atto594 NB120-6405PEATT594
PE/Cy5.5 NB120-6405PECY55
PE/Cy7 NB120-6405PECY7
PerCP NB120-6405PCP

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Blogs on MHC Class I.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

Major Histocompatibility Complex (MHC) Class I
The products of MHC genes are antigen-presenting molecules (APMs) designed for antigen fragment (peptide) presentation to the T-cell receptor. In particular, MHC Class I molecules play a key role in the immune system by presenting endogenously synthes...  Read full blog post.

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Bioinformatics

Gene Symbol HLA-E