MHC Class I Antibody (OX18)

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Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - IHC analysis of formalin fixed paraffin-embedded (FFPE) rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer ...read more
Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).
Flow (Intracellular): MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were stained with MHC Class I NB120-6405 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications B/N, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
OX18
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MHC Class I Antibody (OX18) Summary

Immunogen
Rat spleen glycoproteins
Localization
Cell Membrane; Type I membrane protein.
Specificity
Recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognised.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HLA-E
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blocking/Neutralizing
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
The epitope recognised by this anti-rat RT1-A antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24678820). ELISA reported in scientific literature (PMID 2783579).. Use in blocking/neutralizing reported in scientific literature (PMID 1698855).
Publications
Read Publications using
NB120-6405 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for MHC Class I Antibody (OX18)

  • Clone OX18
  • DKFZp686P19218
  • EA1.2
  • EA2.1
  • HLA class I histocompatibility antigen, alpha chain E
  • HLA class I histocompatibility antigen, E alpha chain
  • HLA-6.2
  • HLAE
  • lymphocyte antigen
  • major histocompatibility complex, class I, E
  • MHC class I antigen E
  • MHC Class I RT1-A
  • MHC HLA-E alpha-1
  • MHC HLA-E alpha-2.1
  • MHC
  • OX18
  • QA1
  • RT1-A (OX18)
  • RT1-A

Background

MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MHC Class I Antibody (NB120-6405)(29)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 5 applications: B/N, FLOW, ICC/IF, IHC-Fr, IP.


Filter By Application
B/N
(1)
FLOW
(2)
ICC/IF
(2)
IHC-Fr
(5)
IP
(2)
All Applications
Filter By Species
Rat
(10)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB120-6405 Applications Species
Stettner M, Lohmann B, Wolffram K et al. Interleukin-17 impedes Schwann cell-mediated myelination. J Neuroinflammation 3/29/2014 [PMID: 24678820] (ICC/IF, Rat) ICC/IF Rat
Bereczky-Veress B, Abdelmagid N, Piehl F et al. Influence of perineurial cells and Toll-like receptors 2 and 9 on Herpes simplex type 1 entry to the central nervous system in rat encephalitis. PLoS One. 2010 Aug 27 [PMID: 20806060] (IHC-Fr, Rat) IHC-Fr Rat
Baca Jones, CC et al. Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells. 2009 [PMID: 19349057]
Zhai, Y Knechtle, S et al. Two distinct forms of soluble MHC class I molecules synthesized by different mechanisms in normal rat cells in vitro. 1998 [PMID: 9684990]
Morrice NA, Powis SJ. A role for the thiol-dependent reductase ERp57 in the assembly of MHC class I molecules. Curr Biol. 1998 Jun 4 [PMID: 9637924] (IP, Rat) IP Rat
Lassmann H, Schmied M, Vass K, Hickey WF. Bone marrow derived elements and resident microglia in brain inflammation. Glia. 1993 Jan 1 [PMID: 7678581]
Mayrhofer G, Schon-Hegrad MA. Ia antigens in rat kidney, with special reference to their expression in tubular epithelium. J Exp Med. 1983 Jun 1 [PMID: 6406641] (IHC-Fr, Rat) IHC-Fr Rat
Mayrhofer G, Pugh CW, Barclay AN. The distribution, ontogeny and origin in the rat of Ia-positive cells with dendritic morphology and of Ia antigen in epithelia, with special reference to the intestine. Eur J Immunol. 1983 Feb 1 [PMID: 6403355]
Fukumoto, T et al. Mouse monoclonal antibodies against rat major histocompatibility antigens. 1982 [PMID: 6178598]
Bukovsky, A et al. Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy. 1984 [PMID: 6146566]
Show All 29 Publications.

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Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for MHC Class I Antibody (NB120-6405) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB120-6405AF405
Alexa Fluor 488 Labeled NB120-6405AF488
Alexa Fluor 647 Labeled NB120-6405AF647
Alexa Fluor 700 Labeled NB120-6405AF700
Allophycocyanin Labeled NB120-6405APC
DyLight 350 Labeled NB120-6405UV
DyLight 405 Labeled NB120-6405V
DyLight 488 Labeled NB120-6405G
DyLight 680 Labeled NB120-6405FR
DyLight 755 Labeled NB120-6405IR
PE Labeled NB120-6405PE
PerCP Labeled NB120-6405PCP

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Blogs on MHC Class I.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

Major Histocompatibility Complex (MHC) Class I
The products of MHC genes are antigen-presenting molecules (APMs) designed for antigen fragment (peptide) presentation to the T-cell receptor. In particular, MHC Class I molecules play a key role in the immune system by presenting endogenously synthes...  Read full blog post.

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Bioinformatics

Gene Symbol HLA-E