Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - IHC analysis of formalin fixed paraffin-embedded (FFPE) rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer ...read more
Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).
Flow (Intracellular): MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were stained with MHC Class I NB120-6405 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then ...read more
The epitope recognized by this anti-rat RT1-A antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24678820). ELISA reported in scientific literature (PMID 2783579). Use in blocking/neutralizing reported in scientific literature (PMID 1698855). This antibody is CyTOF ready.
Read Publications using NB120-6405 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for MHC Class I Antibody (OX18)
HLA class I histocompatibility antigen, alpha chain E
HLA class I histocompatibility antigen, E alpha chain
major histocompatibility complex, class I, E
MHC class I antigen E
MHC Class I RT1-A
MHC HLA-E alpha-1
MHC HLA-E alpha-2.1
MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
MHC Class I and the Herpes Simplex Virus MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens. These molecules are highly polymorphic, meaning that they exist in multiple varian... Read full blog post.
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