MHC Class I Antibody (OX18)


Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The more
Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).
Flow (Intracellular): MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were stained with MHC Class I NB120-6405 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then more

Product Details

Reactivity RtSpecies Glossary
Applications B/N, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IP
1.0 mg/ml

MHC Class I Antibody (OX18) Summary

Rat spleen glycoproteins
Cell Membrane; Type I membrane protein.
Recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognised.
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified


Application Notes
The epitope recognised by this anti-rat RT1-A antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24678820). ELISA reported in scientific literature (PMID 2783579).. Use in blocking/neutralizing reported in scientific literature (PMID 1698855).
Read Publications using
NB120-6405 in the following applications:

Alternate Names for MHC Class I Antibody (OX18)

  • Clone OX18
  • DKFZp686P19218
  • EA1.2
  • EA2.1
  • HLA class I histocompatibility antigen, alpha chain E
  • HLA class I histocompatibility antigen, E alpha chain
  • HLA-6.2
  • HLAE
  • lymphocyte antigen
  • major histocompatibility complex, class I, E
  • MHC class I antigen E
  • MHC Class I RT1-A
  • MHC HLA-E alpha-1
  • MHC HLA-E alpha-2.1
  • MHC
  • OX18
  • QA1
  • RT1-A (OX18)
  • RT1-A


MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, AgAct

Publications for MHC Class I Antibody (NB120-6405)(29)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 5 applications: B/N, FLOW, ICC/IF, IHC-Fr, IP.

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Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB120-6405 Applications Species
Stettner M, Lohmann B, Wolffram K et al. Interleukin-17 impedes Schwann cell-mediated myelination. J Neuroinflammation 3/29/2014 [PMID:24678820] (ICC/IF, Rat) ICC/IF Rat
Bereczky-Veress B, Abdelmagid N, Piehl F et al. Influence of perineurial cells and Toll-like receptors 2 and 9 on Herpes simplex type 1 entry to the central nervous system in rat encephalitis. PLoS One. 2010 Aug 27 [PMID:20806060] (IHC-Fr, Rat) IHC-Fr Rat
Baca Jones, CC et al. Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells. 2009 [PMID:19349057]
Zhai, Y Knechtle, S et al. Two distinct forms of soluble MHC class I molecules synthesized by different mechanisms in normal rat cells in vitro. 1998 [PMID:9684990]
Morrice NA, Powis SJ. A role for the thiol-dependent reductase ERp57 in the assembly of MHC class I molecules. Curr Biol. 1998 Jun 4 [PMID:9637924] (IP, Rat) IP Rat
Lassmann H, Schmied M, Vass K, Hickey WF. Bone marrow derived elements and resident microglia in brain inflammation. Glia. 1993 Jan 1 [PMID:7678581]
Mayrhofer G, Schon-Hegrad MA. Ia antigens in rat kidney, with special reference to their expression in tubular epithelium. J Exp Med. 1983 Jun 1 [PMID:6406641] (IHC-Fr, Rat) IHC-Fr Rat
Mayrhofer G, Pugh CW, Barclay AN. The distribution, ontogeny and origin in the rat of Ia-positive cells with dendritic morphology and of Ia antigen in epithelia, with special reference to the intestine. Eur J Immunol. 1983 Feb 1 [PMID:6403355]
Fukumoto, T et al. Mouse monoclonal antibodies against rat major histocompatibility antigens. 1982 [PMID:6178598]
Bukovsky, A et al. Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy. 1984 [PMID:6146566]
Show All 29 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for MHC Class I Antibody (NB120-6405) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB120-6405AF405
Alexa Fluor (R) 488 Labeled NB120-6405AF488
Alexa Fluor (R) 647 Labeled NB120-6405AF647
Alexa Fluor (R) 700 Labeled NB120-6405AF700
Allophycocyanin Labeled NB120-6405APC
DyLight 350 Labeled NB120-6405UV
DyLight 405 Labeled NB120-6405V
DyLight 405LS Labeled NB120-6405V3
DyLight 488 Labeled NB120-6405G
DyLight 680 Labeled NB120-6405FR
DyLight 755 Labeled NB120-6405IR
PE Labeled NB120-6405PE
PerCP Labeled NB120-6405PCP

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Blogs on MHC Class I.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

Major Histocompatibility Complex (MHC) Class I
The products of MHC genes are antigen-presenting molecules (APMs) designed for antigen fragment (peptide) presentation to the T-cell receptor. In particular, MHC Class I molecules play a key role in the immune system by presenting endogenously synthes...  Read full blog post.

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Gene Symbol HLA-E

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