MHC Class I Antibody (OX18) - BSA Free


Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, more

Product Details

Reactivity RtSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, IP, MS, B/N, CyTOF-ready
BSA Free
1.0 mg/ml

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MHC Class I Antibody (OX18) - BSA Free Summary

Rat spleen glycoproteins
Cell Membrane; Type I membrane protein
Recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognised.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Block/Neutralize reported in scientific literature (PMID 1698855)
  • CyTOF-ready
  • Electron Microscopy reported in scientific literature (PMID 3044648)
  • ELISA 1:100-1:2000. Use reported in scientific literature (PMID 2783579)
  • Flow (Intracellular)
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500. Use reported in scientific literature (PMID 24678820)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
The epitope recognized by this anti-rat RT1-A antibody may be sensitive to formaldehyde fixation and tissue processing. If possible, the use of acetone fixation for frozen sections is recommended. This antibody is CyTOF ready.
Read Publications using
NB120-6405 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for MHC Class I Antibody (OX18) - BSA Free

  • Clone OX18
  • DKFZp686P19218
  • EA1.2
  • EA2.1
  • HLA class I histocompatibility antigen, alpha chain E
  • HLA class I histocompatibility antigen, E alpha chain
  • HLA-6.2
  • HLAE
  • lymphocyte antigen
  • major histocompatibility complex, class I, E
  • MHC class I antigen E
  • MHC Class I RT1-A
  • MHC HLA-E alpha-1
  • MHC HLA-E alpha-2.1
  • MHC
  • OX18
  • QA1
  • RT1-A (OX18)
  • RT1-A


MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MHC Class I Antibody (NB120-6405)(33)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 5 applications: B/N, FLOW, ICC/IF, IHC-Fr, IP.

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Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NB120-6405 Applications Species
Stet, RJM et al. U9F4: A monoclonal antibody recognizing a rat polymorphic class I determinant. 1987-01-01
Farahi S, Hosseini S, Ghanbarian H et al. The Use of Trichostatin A during Pluripotent Stem Cell Generation Does Not Affect MHC Expression Level Stem Cells International 2022-02-15 [PMID: 35371264] (FLOW) FLOW
Smith TA, Ghergherehchi CL, Mikesh M et al. Polyethylene glycol-fusion repair of sciatic allografts in female rats achieves immunotolerance via attenuated innate and adaptive responses J. Neurosci. Res. 2020-09-15 [PMID: 32931034]
Rabenstein M, Unverricht-Yeboah M, Keuters MH et al. Transcranial Current Stimulation Alters the Expression of Immune-Mediating Genes Front Cell Neurosci. 2019-10-25 [PMID: 31708742] (IHC-Fr, Rat) IHC-Fr Rat
Bereczky-Veress B, Abdelmagid N, Piehl F et al. Influence of perineurial cells and Toll-like receptors 2 and 9 on Herpes simplex type 1 entry to the central nervous system in rat encephalitis. PLoS One. 2010-08-27 [PMID: 20806060] (IHC-Fr, Rat) IHC-Fr Rat
Baca Jones, CC et al. Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells. 2009-01-01 [PMID: 19349057]
Zhai, Y Knechtle, S et al. Two distinct forms of soluble MHC class I molecules synthesized by different mechanisms in normal rat cells in vitro. 1998-01-01 [PMID: 9684990]
Morrice NA, Powis SJ. A role for the thiol-dependent reductase ERp57 in the assembly of MHC class I molecules. Curr Biol. 1998-06-04 [PMID: 9637924] (IP, Rat) IP Rat
Lassmann H, Schmied M, Vass K, Hickey WF. Bone marrow derived elements and resident microglia in brain inflammation. Glia. 1993-01-01 [PMID: 7678581]
Mayrhofer G, Pugh CW, Barclay AN. The distribution, ontogeny and origin in the rat of Ia-positive cells with dendritic morphology and of Ia antigen in epithelia, with special reference to the intestine. Eur J Immunol. 1983-02-01 [PMID: 6403355]
Show All 33 Publications.

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Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

Major Histocompatibility Complex (MHC) Class I
The products of MHC genes are antigen-presenting molecules (APMs) designed for antigen fragment (peptide) presentation to the T-cell receptor. In particular, MHC Class I molecules play a key role in the immune system by presenting endogenously synthes...  Read full blog post.

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Gene Symbol HLA-E