Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - 4T1 tumor sections were stained for CD31 (green) and CD105 (red).
Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS-1 Cells with CD31 (MEC13.3) antibody NB600-1475 (blue) and a matched isotype control (orange). Cells were incubated ...read more
Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - C57BL/6 mouse splenocytes stained with purified MEC13.3, followed by anti-rat IgG FITC
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - CD31/PECAM1 (MEC13.3) antibody was tested in Wehi-3 cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained ...read more
Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - Staining of mouse CD31 in non-small cell lung cancer tumor sections from a human NSCLC tumor-bearing mouse. This image was submitted via ...read more
Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on WEHI-3 Cells with CD31 (MEC13.3) antibody NB600-1475 (blue) and a matched isotype control (orange). Cells were ...read more
Hu, MuSpecies Glossary
Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, In vivo, IP, CyTOF-ready
This CD31/PECAM1 Antibody (MEC13.3) is useful for Flow Cytometry, Immunoprecipitation, Immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and in vitro and in vivo blocking of CD31-mediated cell-cell interactions. Please Note: NB600-1475 works well on acetone-fixed frozen sections as well as zinc-fixed paraffin-embedded sections. However, it is not recommended for formalin-fixed paraffin-embedded sections due to inconsistent results. In vivo assay was reported in scientific literature. Use in vitro assays reported in scientific literature (PMID 24647208). This antibody is CyTOF ready.
Read 2 Reviews rated 5 using NB600-1475 in the following applications:
Mouse and Human reactivity reported in scientific literature.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for CD31/PECAM-1 Antibody (MEC13.3)
mec 13.3 cd31
mec 13.3 clone
platelet endothelial cell adhesion molecule
platelet/endothelial cell adhesion molecule
CD31/PECAM1 is commonly used as an endothelial marker, particularly in angiogenesis. CD31 is important in assisting placental and endometrial vascular development. However, CD31 is also associated with many negative angiogenic occurrences, particularly those within or related to tumors and other disease lesions. In MS, plasma microparticules carry CD31 and are important in the formation of brain lesions associated with MS. In cardiac angiosarcoma, CD31 presence is indicative of the severity of the disease. In prostate tumor cells, CD31 is upregulated and associated with increased levels of HIF1a, VEGF, and other hypoxic markers. This relationship seems to indicate that when prostate cancer reaches a sufficient state of development that intense angiogenesis must occur in order to support the ongoing growth and survival of the cancer cells. In all, CD31 is a great marker for normal and disease-induced angiogenesis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for CD31/PECAM-1 Antibody (NB600-1475). (Showing 1 - 1 of 1 FAQs).
I like to know that CD31/PECAM1 Antibody (MEC13.3) (NB600-1475), will work with NBF fixed paraffin embedded sample, not zinc fixed.
It looks like NB600-1475 can be used in IHC of acetone-fixed frozen sections and zinc-fixed paraffin embedded sections. However, it is not recommended for formalin-fixed paraffin-embedded sections due to inconsistent results.
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