CD31/PECAM-1 Antibody (MEC13.3)

Images

 
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - 4T1 tumor sections were stained for CD31 (green) and CD105 (red).
Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS-1 Cells with CD31 (MEC13.3) antibody NB600-1475 (blue) and a matched isotype control (orange). Cells were incubated ...read more
Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - C57BL/6 mouse splenocytes stained with purified MEC13.3, followed by anti-rat IgG FITC
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - CD31/PECAM1 (MEC13.3) antibody was tested in Wehi-3 cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained ...read more
Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - Staining of mouse CD31 in non-small cell lung cancer tumor sections from a human NSCLC tumor-bearing mouse. This image was submitted via ...read more
Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on WEHI-3 Cells with CD31 (MEC13.3) antibody NB600-1475 (blue) and a matched isotype control (orange). Cells were ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, In vivo, IP, CyTOF-ready
Clone
MEC13.3
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CD31/PECAM-1 Antibody (MEC13.3) Summary

Immunogen
Mouse endothelial cell line T-end
Localization
Membrane; Single-pass type I membrane protein. Cell junction.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
PECAM1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:2000
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
  • In vivo assay
  • CyTOF-ready
  • Flow (Cell Surface)
Application Notes
This CD31/PECAM1 Antibody (MEC13.3) is useful for Flow Cytometry, Immunoprecipitation, Immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and in vitro and in vivo blocking of CD31-mediated cell-cell interactions. Please Note: NB600-1475 works well on acetone-fixed frozen sections as well as zinc-fixed paraffin-embedded sections. However, it is not recommended for formalin-fixed paraffin-embedded sections due to inconsistent results. In vivo assay was reported in scientific literature. Use in vitro assays reported in scientific literature (PMID 24647208). This antibody is CyTOF ready.
Reviewed Applications
Read 2 Reviews rated 5
using
NB600-1475 in the following applications:

Publications
Read Publications using
NB600-1475 in the following applications:

Reactivity Notes

Mouse and Human reactivity reported in scientific literature.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD31/PECAM-1 Antibody (MEC13.3)

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • endoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • mec 13.3 cd31
  • mec 13.3 clone
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet/endothelial cell adhesion molecule

Background

CD31/PECAM1 is commonly used as an endothelial marker, particularly in angiogenesis. CD31 is important in assisting placental and endometrial vascular development. However, CD31 is also associated with many negative angiogenic occurrences, particularly those within or related to tumors and other disease lesions. In MS, plasma microparticules carry CD31 and are important in the formation of brain lesions associated with MS. In cardiac angiosarcoma, CD31 presence is indicative of the severity of the disease. In prostate tumor cells, CD31 is upregulated and associated with increased levels of HIF1a, VEGF, and other hypoxic markers. This relationship seems to indicate that when prostate cancer reaches a sufficient state of development that intense angiogenesis must occur in order to support the ongoing growth and survival of the cancer cells. In all, CD31 is a great marker for normal and disease-induced angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD31/PECAM-1 Antibody (NB600-1475)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: FLOW, ICC/IF, IHC, IHC-P, In vivo, in-vitro.


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FLOW
(2)
ICC/IF
(3)
IHC
(2)
IHC-P
(1)
In vivo
(2)
in-vitro
(1)
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Filter By Species
Human
(2)
Mouse
(8)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB600-1475 Applications Species
Abraham V, Cao G, Parambath A et al. Involvement of TIMP-1 in PECAM-1-mediated tumor dissemination Int. J. Oncol. May 25 2018 [PMID: 29845213] (Human) Human
Perales-Puchalt A, Perez-Sanz J, Payne KK et al. Frontline Science: Microbiota reconstitution restores intestinal integrity after cisplatin therapy. J. Leukoc. Biol. Mar 14 2018 [PMID: 29537705] (IHC) IHC
Hernandez R, England CG, Yang Y et al. ImmunoPET imaging of tissue factor expression in pancreatic cancer with (89)Zr-Df-ALT-836. J Control Release 2017 Aug 24 [PMID: 28843831] (Mouse) Mouse
England C, Jiang D, Hernandez R et al. ImmunoPET Imaging of CD146 in Murine Models of Intrapulmonary Metastasis of Non-Small Cell Lung Cancer. Mol Pharm 2017 Sep 06 [PMID: 28825843] (ICC/IF, FLOW, Human) ICC/IF, FLOW Human
DeMars KM, Pacheco SC, Yang C et al. Selective Inhibition of Janus Kinase 3 Has No Impact on Infarct Size or Neurobehavioral Outcomes in Permanent Ischemic Stroke in Mice. Front Neurol 2017 Aug 09 [PMID: 28790974] (ICC/IF, Mouse) ICC/IF Mouse
Takeda A, Hossain MS, Rantakari P et al. Thymocytes in Lyve1-CRE/S1pr1(f/f) Mice Accumulate in the Thymus due to Cell-Intrinsic Loss of Sphingosine-1-Phosphate Receptor Expression. Front Immunol. Nov 23 2016 [PMID: 27877175] (IHC, Mouse) IHC Mouse
Salatino M, Croci DO, Laderach DJ et al. Regulation of galectins by hypoxia and their relevance in angiogenesis: strategies and methods. Methods Mol. Biol. 2014 Sep 25 [PMID: 25253148]
Takeuchi K, Yanai R, Kumase F et al. EGF-Like-Domain-7 Is Required for VEGF-Induced Akt/ERK Activation and Vascular Tube Formation in an Ex Vivo Angiogenesis Assay. PLoS ONE 3/20/2014 [PMID: 24647208] (ICC/IF, in-vitro, Mouse) ICC/IF, in-vitro Mouse
Voswinckel R, Ziegelhoeffer T, Heil M et al. Circulating vascular progenitor cells do not contribute to compensatory lung growth. Circ Res;93(4):372-9. 2003 Aug 22. [PMID: 12881479] (IHC-P, Mouse) IHC-P Mouse
DeLisser HM, Christofidou-Solomidou M, Strieter RM, Burdick MD, Robinson CS, Wexler RS, Kerr JS, Garlanda C, Merwin JR, Madri JA, Albelda SM. Involvement of endothelial PECAM-1/CD31 in angiogenesis. Am J Pathol;151(3):671-7. 1997 Sep. [PMID: 9284815] (In vivo, Mouse) In vivo Mouse
Show All 14 Publications.

Reviews for CD31/PECAM-1 Antibody (NB600-1475) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB600-1475:
Filter by Applications
IHC-Fr
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen CD31/PECAM-1 NB600-1475
Enlarge
reviewed by:
Anonymous
IHC-Fr Human 08/29/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedNon-small cell lung cancer
SpeciesHuman
LotD-2
Immunohistochemistry-Frozen CD31/PECAM-1 NB600-1475
Enlarge
reviewed by:
Christopher England
IHC-Fr Mouse 02/24/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested4T1 tissue
SpeciesMouse

Comments

CommentsGreen is the CD31, which looked perfect!

Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for CD31/PECAM-1 Antibody (NB600-1475). (Showing 1 - 1 of 1 FAQs).

  1. I like to know that CD31/PECAM1 Antibody (MEC13.3) (NB600-1475), will work with NBF fixed paraffin embedded sample, not zinc fixed.
    • It looks like NB600-1475 can be used in IHC of acetone-fixed frozen sections and zinc-fixed paraffin embedded sections. However, it is not recommended for formalin-fixed paraffin-embedded sections due to inconsistent results.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-1475AF405
Alexa Fluor 488 Labeled NB600-1475AF488
Alexa Fluor 647 Labeled NB600-1475AF647
Alexa Fluor 700 Labeled NB600-1475AF700
Allophycocyanin Labeled NB600-1475APC
DyLight 350 Labeled NB600-1475UV
DyLight 405 Labeled NB600-1475V
DyLight 488 Labeled NB600-1475G
DyLight 550 Labeled NB600-1475R
DyLight 650 Labeled NB600-1475C
DyLight 680 Labeled NB600-1475FR
DyLight 755 Labeled NB600-1475IR
FITC Labeled NB600-1475F
PE Labeled NB600-1475PE
PerCP Labeled NB600-1475PCP

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Bioinformatics Tool for CD31/PECAM-1 Antibody (NB600-1475)

Discover related pathways, diseases and genes to CD31/PECAM-1 Antibody (NB600-1475). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for CD31/PECAM-1 Antibody (NB600-1475)

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Blogs on CD31/PECAM-1.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
08/29/2017
Application: IHC-Fr
Species: Human

Christopher England
02/24/2017
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol PECAM1