Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] (NB7115)

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Western Blot: Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] [NB7115] - Detection of DDK in SUSD3 overexpression lysate using anti-DYKDDDDK Epitope Tag antibody (cat.# NBP1-06712) followed by goat anti-rat IgG HRP ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Order Details

Goat anti-Rat IgG (H L) Secondary Antibody [HRP] Summary

Immunogen
Rat IgG-heavy and light chain
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with Rat IgG and with light chains common to other Rat immunoglobulins. No was detected against non-immunoglobulin serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:50000
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/Immunofluorescence 1:200-1:5000
  • Immunohistochemistry 1:200-1:5000
  • Immunohistochemistry-Paraffin 1:200-1:5000
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NB7115 in the following applications:

Publications
Read Publications using NB7115.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS) containing 0.2%BSA
Preservative
0.1% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7115)(2)

Review for IgG (H+L) Antibody (NB7115) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB7115:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot IgG (H+L) NB7115
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reviewed by:
Anonymous
WB Human 03/13/2015
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Summary

ApplicationWestern Blot
Sample TestedSUSD3 Overexpression Lysate, NBL1-16623
SpeciesHuman
LotP18

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratio1:1000 DDK antibody Novus NBP1-06712SS, overnight at 4C in blocking buffer

Secondary Antibody

Secondary Descriptionanti-rat HRP Novus NB7115
Secondary Manufacturer Cat#NB7115
Secondary Concentration1:5000

Details

Detection Notes15-second exposure shows strong band at correct MW.

Comments

CommentsThis anti-rat secondary antibody worked very well, yield strong signal in my western blot. Happy customer!

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB7115) (0)

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Anonymous
03/13/2015
Application: WB
Species: Human