Goat anti-Rat IgG (H+L) Secondary Antibody [HRP]

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Western Blot: Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] [NB7115] - Detection of DDK in SUSD3 overexpression lysate using anti-DYKDDDDK Epitope Tag antibody (cat.# NBP1-06712) followed by Goat anti-Rat IgG (H+L) ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Order Details

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Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] Summary

Immunogen
This Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] was developed against rat IgG-heavy and light chain.
Specificity
By immunoelectrophoresis and ELISA this Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No was detected against non-immunoglobulin serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:50000
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/Immunofluorescence 1:200-1:5000
  • Immunohistochemistry 1:200-1:5000
  • Immunohistochemistry-Paraffin 1:200-1:5000
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NB7115 in the following applications:

Publications
Read Publications using NB7115.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS) containing 0.2%BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7115)(3)

Review for IgG (H+L) Antibody (NB7115) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB7115:
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Images Ratings Applications Species Date Details
Western Blot IgG (H+L) NB7115
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reviewed by:
Anonymous
WB Human 03/13/2015
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Summary

ApplicationWestern Blot
Sample TestedSUSD3 Overexpression Lysate, NBL1-16623
SpeciesHuman
LotP18

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratio1:1000 DDK antibody Novus NBP1-06712SS, overnight at 4C in blocking buffer

Secondary Antibody

Secondary Descriptionanti-rat HRP Novus NB7115
Secondary Manufacturer Cat#NB7115
Secondary Concentration1:5000

Details

Detection Notes15-second exposure shows strong band at correct MW.

Comments

CommentsThis anti-rat secondary antibody worked very well, yield strong signal in my western blot. Happy customer!

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB7115) (0)

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Other Available Formats

Alkaline Phosphatase NB7113
FITC NB7114
HRP NB7115

Additional IgG (H+L) Products

Array NB7115

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Recent Reviews

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Anonymous
03/13/2015
Application: WB
Species: Human