c-Myc Antibody (9E10) [PE]

Images

 
Flow Cytometry: c-Myc Antibody (9E10) [PE] [NB600-302PE] - An intracellular stain was performed on Jurkat cells with c-MYC antibody (9E10) NB600-302PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells ...read more

Product Details

Summary
Reactivity Hu, Mu, DrSpecies Glossary
Applications Flow
Clone
9E10
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

c-Myc Antibody (9E10) [PE] Summary

Immunogen
A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) of human c-Myc. [UniProt# P01106]
Epitope
Leucine zipper sequence (AEEQKLISEEDLL)
Specificity
Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
This c-Myc antibody clone 9E10 is useful for Flow Cytometry (PMID: 21315712), Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Immunoprecipitation.
Reviewed Applications
Read 1 Review rated 4
using
NB600-302PE in the following application:

Reactivity Notes

Human, mouse and Drosophila.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for c-Myc Antibody (9E10) [PE]

  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • cMyc
  • c-Myc
  • myc proto-oncogene protein
  • Myc
  • Myc2
  • MYCC
  • myc-related translation/localization regulatory factor
  • Niard
  • Nird
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)

Background

Myc genes are a family of proto-oncogenes (L- Myc, N- Myc and C- Myc) that codes for Myc proteins which are transcriptor factors implicated in cellular proliferation, differentiation, apoptosis, metabolism, adhesion and self-renovation of tumor stem cells. Myc protein can act as transcriptional activator/repressor, and is activated via response to diverse mitogenic signals (including Wnt, Shh and EGF) and has been found to be up-regulated in several types of cancers. c-Myc participates gene transcription regulation and binds DNA in a non-specific manner, yet can specifically recognizes core sequence 5'-CAC[GA]TG-3' also. c-Myc heterodimerization with another bHLH protein namely Myc-associated factor X (MAX) is required for efficient c-Myc- DNA binding. c-Myc interacts with several proteins such as TAF1C, SPAG9, PARP10, KDM5A, KDM5B, NO66, PIM2 and with FBXW7 when phosphorylated at Thr-58/Ser-62. c-Myc activate the transcription of growth-related genes and c- Myc overexpression induce cell-cycle progression thereby implicating in a variety of cancers. Moreover, a chromosomal aberration involving c-Myc has been linked to a form of B-cell chronic lymphocytic leukemia and defective c-MYC is responsible for Burkitt lymphoma also.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-302PE
Species: Hu, Mu, Dr
Applications: Flow

Publications for c-Myc Antibody (NB600-302PE) (0)

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Review for c-Myc Antibody (NB600-302PE) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow.

Reviews using NB600-302PE:
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Images Ratings Applications Species Date Details
Flow Cytometry c-Myc NB600-302PE
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reviewed by:
Adrienne Greenough
Flow Mouse 07/03/2014
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Summary

ApplicationFlow Cytometry
Sample TestedSplenocytes
SpeciesMouse

Product General Protocols

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FAQs for c-Myc Antibody (NB600-302PE) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB600-302APC
Biotin Labeled NB600-302B
DyLight 350 Labeled NB600-302UV
DyLight 405 Labeled NB600-302V
DyLight 488 Labeled NB600-302G
DyLight 550 Labeled NB600-302R
DyLight 650 Labeled NB600-302C
DyLight 680 Labeled NB600-302FR
DyLight 755 Labeled NB600-302IR
FITC Labeled NB600-302F
HRP Labeled NB600-302H
PerCP Labeled NB600-302PCP

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Blogs on c-Myc. Showing 1-10 of 14 blog posts - Show all blog posts.

The role of c-Fos in the regulation of the JC virus gene transcription
c-Fos is a member of the AP-1 transcription factor family under the Fos protein family umbrella, alongside Fra-1, Fra-2 and Fos-B.  Also in the AP-1 transcription family are the Jun proteins, c-Jun, Jun-B and Jun-D.  Each member of the AP-1 transcri...  Read full blog post.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

MYC - A human oncogene with valuable laboratory applications
Myc is a basic helix-loop-helix zipper transcription factor that regulates a network of many hundreds of genes. Myc up-regulates the expression of many genes involved in cell growth and proliferation such as ribosome biogenesis and protein synthesi...  Read full blog post.

c-Myc - transcription factor and oncogene
c-Myc is a protein of the Myc family of transcription factors (c-Myc, B-Myc, L-Myc, N-Myc, and s-Myc) encoded by the MYC proto-oncogene. c-Myc was first discovered as the cellular homolog of the retroviral v-Myc oncogene. c-Myc is a transcription...  Read full blog post.

c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample on...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2A....  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

Showing 1-10 of 14 blog posts - Show all blog posts.

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Recent Reviews

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5
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4
1
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2
0
1
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Adrienne Greenough
07/03/2014
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol MYC
Entrez