beta-Actin Antibody

Images

 
Western Blot: beta-Actin Antibody [NB600-532] - Detection of actin in 3T3 (20ug) lysates ECL detection 30 seconds. Lane 1 - 1:15,000 dilution Lane 2 - 1:10,000 dilution Lane 3 - 1:15,000 dilution.
Western Blot: beta-Actin Antibody [NB600-532] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Simple Western: beta-Actin Antibody [NB600-532] - Simple Western lane view shows a specific band for Beta Actin in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Western Blot: beta-Actin Antibody [NB600-532] - Western blot analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].
Immunohistochemistry: beta-Actin Antibody [NB600-532] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: beta-Actin Antibody [NB600-532] - Western blot analysis of Actin (NB600-532) using RCC4 whole cell lysate [NBP1-30412].
Western Blot: beta-Actin Antibody [NB600-532] - Detection of Beta Actin. Lane 1: 5ug, nuclear extract from mouse bone marrow derived macrophages. Lane 2: 10ug, cytoplasmic extract from mouse bone narrow derived ...read more
Western Blot: beta-Actin Antibody [NB600-532] - Analysis using the HRP conjugate of NB600-532. Detection of Beta Actin in MCF-7 cell lysate (20ug) using anti-Beta Actin antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq, Fi, GP, Pm, RM, Sh, Tr, XpSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

beta-Actin Antibody Summary

Immunogen
A region corresponding to the N-terminus of human Beta Actin [UniProt# P60709]
Predicted Species
Rat (100%), Porcine (100%), Primate (100%), Fish (100%), Turkey (100%), Sheep (100%), Xenopus (100%), Chicken (100%), Canine (100%), Guinea Pig (100%), Equine (100%), Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACTB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Simple Western 1:2000
  • Immunohistochemistry
Application Notes
This antibody is useful for Western Blot. A 40 kDa band is detected in HeLa whole cell lysate and mouse NIH3T3 cells. This antibody is also useful for immunocytochemistry/immunofluorescence.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 5 Reviews rated 4.4
using
NB600-532 in the following applications:

Publications
Read Publications using
NB600-532 in the following applications:

  • WB
    18 publications

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with X. tropicalis, Panda, Orangutan, Gorilla, Chimpanzee, Medaka fish, Australian taipan, Emu, Duckbill platypus and King baboon spider.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for beta-Actin Antibody

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • Beta Actin
  • beta cytoskeletal actin
  • betaActin
  • beta-Actin
  • BRWS1
  • PS1TP5-binding protein 1
  • PS1TP5BP1

Background

In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. This antibody serves as an excellent loading control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-Actin Antibody (NB600-532)(33)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(18)
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Filter By Species
Human
(14)
Mouse
(7)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NB600-532 Applications Species
Frey-Jakobs S, Hartberger JM, Fliegauf M et al. ZNF341 controls STAT3 expression and thereby immunocompetence Sci Immunol 43266 [PMID: 29907690] (WB, Human) WB Human
Jarc E, Kump A, Malavasic P et al. Lipid droplets induced by secreted phospholipase A2 and unsaturated fatty acids protect breast cancer cells from nutrient and lipotoxic stress. Biochim. Biophys. Acta 2017 Dec 09 [PMID: 29229414] (Human) Human
Moriwaki M, Moore B, Mosbruger T et al. POLR2C Mutations Are Associated With Primary Ovarian Insufficiency in Women. J Endocr Soc. 2017 Mar 01 [PMID: 29057389] (WB, Human) WB Human
Ueno K, Samura M, Nakamura T et al. Increased plasma VEGF levels following ischemic preconditioning are associated with downregulation of miRNA-762 and miR-3072-5p. Sci Rep. Dec 1 2016 [PMID: 27905554] (WB, Mouse) WB Mouse
Li Y, Li Y, Chen D et al. miR-30a-5p in the tumorigenesis of renal cell carcinoma: A tumor suppressive microRNA. Mol Med Rep. 2016 May 01 [PMID: 27035333]
Park JE, Lee M, Mifflin R, Lee YK. Enhanced ethanol catabolism in orphan nuclear receptor SHP-null mice. Am. J. Physiol. Gastrointest. Liver Physiol. May 15 2016 [PMID: 26968209] (WB, Mouse) WB Mouse
Hosoyama T, Samura M, Kudo T et al. Cardiosphere-derived cell sheet primed with hypoxia improves left ventricular function of chronically infarcted heart. Am J Transl Res. 2015 Dec 15 [PMID: 26885271]

Details:
This citation used the HRP version of this antibody.
Li Y, Chen D, Li Y et al. Oncogenic cAMP responsive element binding protein 1 is overexpressed upon loss of tumor suppressive miR-10b-5p and miR-363-3p in renal cancer. Oncol Rep 2016 Apr [PMID: 26796749]
Samura M, Morikage N, Suehiro K et al. Combinatorial Treatment with Apelin-13 Enhances the Therapeutic Efficacy of a Preconditioned Cell-Based Therapy for Peripheral Ischemia. Sci Rep. 2016 Jan 14 [PMID: 26763337] (WB, Mouse) WB Mouse
Show All 33 Publications.

Reviews for beta-Actin Antibody (NB600-532) (5) 4.45

Average Rating: 4.4
(Based on 5 reviews)
We have 5 reviews tested in 2 species: Human, Mouse.

Reviews using NB600-532:
Filter by Applications
WB
(3)
Simple Western
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot beta-Actin NB600-532
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reviewed by:
Anonymous
WB Human 03/28/2016
View

Summary

ApplicationWestern Blot
Sample TestedHuman ovarian teratocarcinoma
SpeciesHuman
LotLot A

Blocking

Blocking Detailsnon-fat dry milk in TBST, 5%, 2hours, at room temp

Primary Anitbody

Dilution Ratio1:5000, overnight, 4C, 5% non-fat dry milk in TBST

Secondary Antibody

Secondary DescriptionDonkey antirabbit, HRP
Secondary Manufacturer Cat#Life Technologies Catalog#A16023
Secondary Concentration1:2000

Details

Detection NotesCL-Xposure film from Fisher, Incubate with Chemiluminescent ECL solution for 1min and develop in the fixing box, 10min exposure

Comments

CommentsThe target molecular weight was 40kDa.
Simple Western beta-Actin NB600-532
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reviewed by:
Anonymous
Simple Western Mouse 01/21/2016
View

Summary

ApplicationSimple Western
Sample Testedwhole brain homogenates from mice
SpeciesMouse

Blocking

Blocking Detailsn/a

Primary Anitbody

Dilution Ratio1:10,000, 1:5,000, 1:1,000

Details

Detection NotesWes Simple Western platform

Comments

CommentsBest at lower concentrations due to some faint high molecular weight bands.
 
reviewed by:
Sachin Puri
IHC-P Human 07/23/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman Tonsils
SpeciesHuman

Blocking

Blocking Detailshorse blocking serum

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionHRP conjugated anti rabbit IgG
Secondary Concentration1:10000

Details

Detection NotesChemiluminescence
Fixation DetailsAntigen Retrival using microwave
Wash Descriptioncitrate buffer
Western Blot beta-Actin NB600-532
Enlarge
reviewed by:
Panagiota Kafasla
WB Mouse 07/09/2013
View

Summary

ApplicationWestern Blot
Sample TestedMouse total lysate, macrophages
SpeciesMouse
LotA
Commentsa very good, clear antibody, tested in Western blotting. I used it in 4%milk in TBS-T, at a dilution of 1:5000, for 2hrs at RT. Secondary antibody was used at 1:2000 dilution, in TBS-T with 4% milk.

Blocking

Blocking Details4%milk in TBS-T

Primary Anitbody

Dilution Ratio1:5000 in TBS-T 4%milk, 2hr RT

Secondary Antibody

Secondary Descriptionanti-rabbit IgG HRP
Secondary Manufacturer Cat#southern biotech
Secondary Concentration1:2000

Details

Detection NotesECL, Phosphorimager

Comments

Commentsa very good, clear antibody, tested in Western blotting. I used it in 4%milk in TBS-T, at a dilution of 1:5000, for 2hrs at RT. Secondary antibody was used at 1:2000 dilution, in TBS-T with 4% milk.
 
reviewed by:
Izabela Podgorski
WB 07/11/2012
View

Summary

ApplicationWestern Blot
Special ApplicationsWorks great against human and mouse

Product General Protocols

View specific protocols for beta-Actin Antibody (NB600-532): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for beta-Actin Antibody (NB600-532). (Showing 1 - 6 of 6 FAQs).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW of beta Actin antibodies is approximately 40 - 42 kDa.
  5. I would like to know whether the antibody anti-actin (NB600-532) from rabbit can be also used for immunofluorescence and which dilution you recommend?
    • This antibody has never been validated in rabbit. It is listed as being negative for immunocytochemistry/immunofluorescence, so is not recommended for your assay. NB600-501 has been validated in rabbit as well as for ICC/IF. I would highly recommend this product instead of NB600-532. Full information about this product and recommended dilutions can be found on the datasheet. For Immunocytochemistry/Immunofluorescence, we recommend a dilution of 1:1000-1:2000.
  6. What is the immunogen sequence of this Beta-Actin antibody?
    • A sequence within the N-terminal region of Human beta Actin. The exact sequence is proprietary.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-532AF405
Alexa Fluor 488 Labeled NB600-532AF488
Alexa Fluor 647 Labeled NB600-532AF647
Alexa Fluor 700 Labeled NB600-532AF700
Biotin Labeled NB600-532B
DyLight 405 Labeled NB600-532V
DyLight 488 Labeled NB600-532G
DyLight 550 Labeled NB600-532R
DyLight 650 Labeled NB600-532C
FITC Labeled NB600-532F
HRP Labeled NB600-532H

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Bioinformatics Tool for beta-Actin Antibody (NB600-532)

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Blogs on beta-Actin. Showing 1-10 of 245 blog posts - Show all blog posts.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Showing 1-10 of 245 blog posts - Show all blog posts.

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Recent Reviews

4.4
5
3
4
1
3
1
2
0
1
0

Anonymous
03/28/2016
Application: WB
Species: Human

Anonymous
01/21/2016
Application: Simple Western
Species: Mouse

 
Sachin Puri
07/23/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol ACTB
Entrez