This antibody is useful for Western Blot. A 40 kDa band is detected in HeLa whole cell lysate and mouse NIH3T3 cells. This antibody is also useful for immunocytochemistry/immunofluorescence.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Based on 100% sequence identity, this antibody is predicted to react with X. tropicalis, Panda, Orangutan, Gorilla, Chimpanzee, Medaka fish, Australian taipan, Emu, Duckbill platypus and King baboon spider.
Packaging, Storage & Formulations
Store at 4C in the dark.
Immunogen affinity purified
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for beta-Actin Antibody [HRP]
actin, cytoplasmic 1
beta cytoskeletal actin
PS1TP5-binding protein 1
In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. This antibody serves as an excellent loading control.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for beta-Actin Antibody (NB600-532H). (Showing 1 - 5 of 5 FAQs).
Why is beta actin used as a loading control in blotting ?
Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
Are the beta-Actin antibodies validated in Simple Western?
Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
Do Beta-Actin antibodies come in lyophilized format?
ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
What the theoretical molecular weight for Beta-Actin antibodies?
The TMW of beta Actin antibodies is approximately 40 - 42 kDa.
What is the immunogen sequence of this Beta-Actin antibody?
A sequence within the N-terminal region of Human beta Actin. The exact sequence is proprietary.
Beta-Actin's Role in Neuronal Plasticity Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro... Read full blog post.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.