beta-Actin Antibody [HRP]

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Western Blot: Beta Actin Antibody [HRP] [NB600-532H] - analysis of Beta Actin in MCF-7 cell lysate (20ug) using anti-Beta Actin antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq, Fi, GP, Pm, RM, Sh, Tr, XpSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

beta-Actin Antibody [HRP] Summary

Immunogen
A region corresponding to the N-terminus of human Beta Actin [UniProt# P60709]
Predicted Species
Rat (100%), Porcine (100%), Xenopus (100%), Rhesus Macaque (100%), Turkey (100%), Equine (100%), Bovine (100%), Fish (100%), Sheep (100%), Canine (100%), Chicken (100%), Guinea Pig (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACTB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Immunoprecipitation 2-5 ug/mg
Application Notes
This antibody is useful for Western Blot. A 40 kDa band is detected in HeLa whole cell lysate and mouse NIH3T3 cells. This antibody is also useful for immunocytochemistry/immunofluorescence.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System
Reviewed Applications
Read 1 Review rated 4
using
NB600-532H in the following applications:

Publications
Read Publications using
NB600-532H in the following applications:

  • WB
    1 publication

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with X. tropicalis, Panda, Orangutan, Gorilla, Chimpanzee, Medaka fish, Australian taipan, Emu, Duckbill platypus and King baboon spider.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for beta-Actin Antibody [HRP]

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • Beta Actin
  • beta cytoskeletal actin
  • betaActin
  • beta-Actin
  • BRWS1
  • PS1TP5-binding protein 1
  • PS1TP5BP1

Background

In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. This antibody serves as an excellent loading control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-Actin Antibody (NB600-532H)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Review for beta-Actin Antibody (NB600-532H) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-532H:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot beta-Actin NB600-532H
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reviewed by:
Anonymous
WB Human 10/16/2014
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Summary

ApplicationWestern Blot
Sample TestedCell lysate from MCF7 cells grown in DMEM medium
SpeciesHuman
CommentsHaving a primary antibody with HRP conjugation simplify the task and this one works perfect.

Blocking

Blocking DetailsNon-fat dry milk (5%) in 1X PBS

Primary Anitbody

Dilution Ratio1:800 in PBS at RT for 3 hours

Secondary Antibody

Secondary DescriptionAnti rabbit IgG (HRP-conjugated)
Secondary Manufacturer Cat#ab6789 (Abcam)
Secondary Concentration1:10K

Details

Detection NotesBio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 20 sec

Comments

CommentsHaving a primary antibody with HRP conjugation simplify the task and this one works perfect.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for beta-Actin Antibody (NB600-532H). (Showing 1 - 5 of 5 FAQs).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW of beta Actin antibodies is approximately 40 - 42 kDa.
  5. What is the immunogen sequence of this Beta-Actin antibody?
    • A sequence within the N-terminal region of Human beta Actin. The exact sequence is proprietary.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB600-532B

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Bioinformatics Tool for beta-Actin Antibody (NB600-532H)

Discover related pathways, diseases and genes to beta-Actin Antibody (NB600-532H). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for beta-Actin Antibody (NB600-532H)

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Pathways for beta-Actin Antibody (NB600-532H)

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Research Areas for beta-Actin Antibody (NB600-532H)

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Blogs on beta-Actin.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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Recent Reviews

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Anonymous
10/16/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol ACTB
Entrez