Western Blot: beta-Actin Antibody [NB100-56874] - Analysis of HeLa, K562, A431, C2C12, C6, and Rat2 cell lines.
Immunohistochemistry-Paraffin: beta-Actin Antibody [NB100-56874] - analysisof human spleen using antibody at 10 ug/ml.
Western Blot: beta-Actin Antibody [NB100-56874] - Analysis using beta actin antibody: Decreasing amounts of human ovary tissue lysate were probed using beta actin antibody at 0.25 ug/ml: A) 40 ug, B) 30 ug, C) 20 ug, ...read more
Western Blot: beta-Actin Antibody [NB100-56874] - Analysis using beta actin antibody: Lysate from A: human brain, B: mouse brain, C: rat brain, D: human lung and E: human spleen tissue probed using beta actin antibody ...read more
Simple Western: beta-Actin Antibody [NB100-56874] - Simple Western lane view shows a specific band for Beta Actin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
This antibody is not suitable for testing heart or muscle lysate loading. In Simple Western only 10-15 uL of the recommended dilution is used per data point. Use in Immunohistochemistry reported in scientific literature (PMID 27371029).
Read 2 Reviews rated 5 using NB100-56874 in the following application:
Invertebrate reactivity reported in scientific literature (PMID: 25240497). Porcine reactivity reported in scientific literature (PMID: 26593451)
Alternate Names for beta-Actin Antibody
actin, cytoplasmic 1
beta cytoskeletal actin
PS1TP5-binding protein 1
Actin is a globular protein found in all eukaryotic cells (the only known exception being nematode sperm) where it may be present at concentrations of over 100 uM. It is the monomeric subunit of two types of filaments: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. Actin participates in many important cellular processes including muscle contraction, cell signaling, motility and division, cytokinesis, vesicle and organelle movement, and the establishment and maintenance of cell junctions and shape. In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cells as components of the cytoskeleton, and as mediators of internal cell motility (Doherty GJ, et al 2008). Among these actins, beta actin, which is expressed constantly, and at high levels, in all the tissue types used in protein research except muscle (skeletal and cardiac). Beta actin acts as a useful loading control in Western blot analysis. Loading controls serve a number of purposes; they ensure the protein of interest has been correctly loaded on the gel, that it is being transferred correctly, and that all reagents are functioning normally. Loading controls can also be used to compare amounts of protein tested (or loaded) per gel lane in western blotting. While very useful when comparing lysates of the same origin (for example transfected and untransfected HEK 293s), comparing lysates from different tissues can be complex. Actin is almost universally expressed, but different tissues express different amounts. In western blotting, an actin antibody titered to the detection limit can show these differences, while an actin antibody used well above the detection limit can show a response that appears equally robust in all samples probed. Therefore caution must be used when using actin or another 'loading control antibody.'
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for beta-Actin Antibody (NB100-56874). (Showing 1 - 6 of 6 FAQs).
Why is beta actin used as a loading control in blotting ?
Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
Are the beta-Actin antibodies validated in Simple Western?
Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
Do Beta-Actin antibodies come in lyophilized format?
ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
What the theoretical molecular weight for Beta-Actin antibodies?
The TMW of beta Actin antibodies is approximately 40 - 42 kDa.
I would like to find the manual description of this product, to confirm what dilution is recommended for Western blot.
The recommended starting dilution for Western blotting is 0.25-1ug/ml, but we suggest that you optimise this for your particular sample set.
What is the immunogen sequence of this Beta-Actin antibody?
A sequence of amino acids 2 - 16 (CDDDIAALVIDNGSG) of beta Actin.
Beta-Actin's Role in Neuronal Plasticity Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro... Read full blog post.