beta-Actin Antibody

Images

 
Simple Western: beta-Actin Antibody [NB100-56874] - Image shows a specific band for Beta Actin in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: beta-Actin Antibody [NB100-56874] - Specific bands are seen in various cell lysates at the same molecular weight. A: human brain, B: mouse brain, C: rat brain, D: human lung, and E: human spleen tissue ...read more
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody [NB100-56874] - Actin was detected in NIH-3T3 cells fixed with methanol using rabbit anti-mouse Actin antibody (NB100-56874) at 1:100 dilution, overnight at ...read more
Western Blot: beta-Actin Antibody [NB100-56874] - Analysis of NS5B protein expression after transfecting NS5B and La autoantigen siRNAs: HCV-infected Huh-7. 5 cells were treated with siRNAs against La autoantigen and ...read more
Immunohistochemistry-Paraffin: beta-Actin Antibody [NB100-56874] - Analysis of human spleen using antibody at 10 ug/mL.
Western Blot: beta-Actin Antibody [NB100-56874] - Decreasing amounts of human ovary tissue lysate were probed using beta actin antibody at 0.25 ug/mL: A) 40 ug, B) 30 ug, C) 20 ug, D) 10 ug per lane.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ChHa, InSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

beta-Actin Antibody Summary

Immunogen
Amino acids 2-16 (CDDDIAALVIDNGSG) of actin protein were used as the immunogen sequence for this beta-Actin Antibody.
Specificity
The NCBI accession number for actin is AAA51567.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACTB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/mL
  • Knockdown Validated
  • Simple Western 1:12.5
  • Western Blot 0.25-1 ug/ml
Application Notes
This antibody is not suitable for testing heart or muscle lysate. Use in Immunohistochemistry reported in scientific literature (PMID 27371029). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Knockdown validation (PMID: 31835509).
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 4
using
NB100-56874 in the following applications:

Publications
Read Publications using
NB100-56874 in the following applications:

  • IHC
    1 publication
  • KD
    1 publication
  • WB
    58 publications

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34443654). Invertebrate, Porcine, Bovine, and Chinese Hamster reactivity reported in scientific literature (PMID: 25240497, 26593451, 18650284, and 19291423 respectively). .

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for beta-Actin Antibody

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • B-Actin
  • Beta Actin
  • beta cytoskeletal actin
  • betaActin
  • beta-Actin
  • BRWS1
  • I(2)-Actin
  • PS1TP5-binding protein 1
  • PS1TP5BP1

Background

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References

1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-Actin Antibody (NB100-56874)(75)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bovine, C. elegans, Chinese hamster, Invertebrate, Porcine.

We have publications tested in 3 applications: IHC, KD, WB.


Filter By Application
IHC
(1)
KD
(1)
WB
(58)
All Applications
Filter By Species
Human
(20)
Mouse
(25)
Rat
(5)
Bovine
(3)
C. elegans
(1)
Chinese hamster
(1)
Invertebrate
(2)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 75. Show All 75 Publications.
Publications using NB100-56874 Applications Species
Chandra K, Roy Chowdhury A, Chatterjee R, Chakravortty D GH18 family glycoside hydrolase Chitinase A of Salmonella enhances virulence by facilitating invasion and modulating host immune responses PLoS pathogens Apr 1 2022 [PMID: 35482710] (WB, C. elegans) WB C. elegans
Mohamed RA, Abdallah DM, El-Brairy AI Et al. Palonosetron/Methyllycaconitine Deactivate Hippocampal Microglia 1, Inflammasome Assembly and Pyroptosis to Enhance Cognition in a Novel Model of Neuroinflammation Molecules (Basel, Switzerland) Aug 21 2021 [PMID: 34443654] (WB, Rat) WB Rat
Tomita K, Nagasawa T, Kuwahara Y Et al. MiR-7-5p Is Involved in Ferroptosis Signaling and Radioresistance Thru the Generation of ROS in Radioresistant HeLa and SAS Cell Lines International journal of molecular sciences Aug 2 2021 [PMID: 34361070] (WB, Human) WB Human
Sato S, Kikuchi T, Nishimura Y et al. Generation of mouse iPS cells using an inducible expression of transgenes via the cumate gene-switch Anal. Biochem. Apr 22 2020 [PMID: 32333903] (WB, Mouse) WB Mouse
Acosta GHG. Susceptibility of parkinson's disease following mild blast traumatic brain injury Thesis. 2014 Aug 01 (WB, Rat) WB Rat
Takashi Y, Tomita K, Kuwahara Y et al. Mitochondrial dysfunction promotes aquaporin expression that controls hydrogen peroxide permeability and ferroptosis Free Radic Biol Med Oct 2 2020 [PMID: 33017631] (WB, Human) WB Human
Wang L, Zhang S, Zhang W et Al. miR-424 Promotes Bovine Adipogenesis Through an Unconventional Post-Transcriptional Regulation of STK11 Front Genet Mar 4 2020 [PMID: 32194625] (WB, Bovine) WB Bovine
Wang L, Zhang S, Cheng G et al. MiR-145 reduces the activity of PI3K/Akt and MAPK signaling pathways and inhibits adipogenesis in bovine preadipocytes Genomics Mar 3 2020 [PMID: 32135297] (WB) WB
Uemasu K, Tanabe N, Tanimura K et Al. Serine Protease Imbalance in the Small Airways and Development of Centrilobular Emphysema in COPD Am. J. Respir. Cell Mol. Biol. Feb 26 2020 [PMID: 32101459] (Mouse) Mouse
Su X, Wang Y, Li A Neudesin Neurotrophic Factor Promotes Bovine Preadipocyte Differentiation and Inhibits Myoblast Myogenesis Animals (Basel) Dec 10 2019 [PMID: 31835509] (WB, KD, Bovine) WB, KD Bovine
Show All 75 Publications.

Reviews for beta-Actin Antibody (NB100-56874) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Feline.

Reviews using NB100-56874:
Filter by Applications
Simple Western
(1)
Western Blot
(3)
All Applications
Filter by Species
Human
(2)
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western beta-Actin NB100-56874
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1
reviewed by:
Danxin Wang
Simple Western Human 03/20/2020
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Summary

ApplicationSimple Western
Sample Testedliver
SpeciesHuman
Lot12221897B-7
Western Blot beta-Actin NB100-56874
Enlarge
5
reviewed by:
Verified Customer
Western Blot Feline 05/18/2017
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Summary

ApplicationWestern Blot
Sample TestedKidney tissue homogenates
SpeciesFeline
Lot12221897B-4
  5
reviewed by:
Verified Customer
Western Blot 09/15/2014
View

Summary

ApplicationWestern Blot
Western Blot beta-Actin NB100-56874
Enlarge
5
reviewed by:
Shilpa Agarwal
Western Blot Human 08/19/2014
View

Summary

ApplicationWestern Blot
Sample TestedLysate from 293T cells
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Actin Antibody (NB100-56874). (Showing 1 - 7 of 7 FAQs).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What is the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW for beta Actin antibodies is approximately 42 kDa.
  5. I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
    • For homogenized tissue, beta-actin and GAPDH are both fine.
  6. I would like to find the manual description of this product, to confirm what dilution is recommended for Western blot.
    • The recommended starting dilution for Western blotting is 0.25-1ug/ml, but we suggest that you optimise this for your particular sample set.
  7. What is the immunogen sequence of this Beta-Actin antibody?
    • A sequence of amino acids 2 - 16 (CDDDIAALVIDNGSG) of beta Actin.

Secondary Antibodies

 

Isotype Controls

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Blogs on beta-Actin.

Application Focus: I see an increase in LC3, now what?
 By Christina Towers, PhD.  Autophagy is highly conserved and tightly regulated process that all cell types use to recycle nutrients, particularly in the instance of stress1. As a result, even sm...  Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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Recent Reviews

4
5
3
4
0
3
0
2
0
1
1

Danxin Wang
03/20/2020
Application: Simple Western
Species: Human

Verified Customer
05/18/2017
Application: Western Blot
Species: Feline

 
Verified Customer
09/15/2014
Application: Western Blot
Species:

Bioinformatics

Gene Symbol ACTB
Entrez