beta-Actin Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody [NB100-56874] - Actin was detected in NIH-3T3 cells fixed with methanol using rabbit anti-mouse Actin antibody (NB100-56874) at 1:100 dilution, overnight at ...read more
Western Blot: beta-Actin Antibody [NB100-56874] - Analysis of HeLa, K562, A431, C2C12, C6, and Rat2 cell lines.
Immunohistochemistry-Paraffin: beta-Actin Antibody [NB100-56874] - analysisof human spleen using antibody at 10 ug/ml.
Western Blot: beta-Actin Antibody [NB100-56874] - Analysis using beta actin antibody: Decreasing amounts of human ovary tissue lysate were probed using beta actin antibody at 0.25 ug/ml: A) 40 ug, B) 30 ug, C) 20 ug, ...read more
Western Blot: beta-Actin Antibody [NB100-56874] - Analysis using beta actin antibody: Lysate from A: human brain, B: mouse brain, C: rat brain, D: human lung and E: human spleen tissue probed using beta actin antibody ...read more
Simple Western: beta-Actin Antibody [NB100-56874] - Simple Western lane view shows a specific band for Beta Actin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, InSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

beta-Actin Antibody Summary

Immunogen
Amino acids 2-16 (CDDDIAALVIDNGSG) of actin protein were used as the immunogen.
Specificity
The NCBI accession number for actin is AAA51567. This can not be used to test heart or muscle lysate loading.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACTB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25-1 ug/ml
  • Simple Western 1:12.5
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
Application Notes
This antibody is not suitable for testing heart or muscle lysate loading. Use in Immunohistochemistry reported in scientific literature (PMID 27371029).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 3 Reviews rated 5
using
NB100-56874 in the following applications:

Publications
Read Publications using
NB100-56874 in the following applications:

  • IHC
    1 publication
  • WB
    41 publications

Reactivity Notes

Invertebrate reactivity reported in scientific literature (PMID: 25240497). Porcine reactivity reported in scientific literature (PMID: 26593451)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for beta-Actin Antibody

  • ACTB
  • actin, beta
  • actin, cytoplasmic 1
  • Beta Actin
  • beta cytoskeletal actin
  • betaActin
  • beta-Actin
  • BRWS1
  • PS1TP5-binding protein 1
  • PS1TP5BP1

Background

Actin is a globular protein found in all eukaryotic cells (the only known exception being nematode sperm) where it may be present at concentrations of over 100 uM. It is the monomeric subunit of two types of filaments: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. Actin participates in many important cellular processes including muscle contraction, cell signaling, motility and division, cytokinesis, vesicle and organelle movement, and the establishment and maintenance of cell junctions and shape. In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cells as components of the cytoskeleton, and as mediators of internal cell motility (Doherty GJ, et al 2008). Among these actins, beta actin, which is expressed constantly, and at high levels, in all the tissue types used in protein research except muscle (skeletal and cardiac). Beta actin acts as a useful loading control in Western blot analysis. Loading controls serve a number of purposes; they ensure the protein of interest has been correctly loaded on the gel, that it is being transferred correctly, and that all reagents are functioning normally. Loading controls can also be used to compare amounts of protein tested (or loaded) per gel lane in western blotting. While very useful when comparing lysates of the same origin (for example transfected and untransfected HEK 293s), comparing lysates from different tissues can be complex. Actin is almost universally expressed, but different tissues express different amounts. In western blotting, an actin antibody titered to the detection limit can show these differences, while an actin antibody used well above the detection limit can show a response that appears equally robust in all samples probed. Therefore caution must be used when using actin or another 'loading control antibody.'

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-Actin Antibody (NB100-56874)(56)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bovine, Chinese hamster, Invertebrate, Porcine.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(1)
WB
(41)
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Filter By Species
Human
(11)
Mouse
(21)
Rat
(4)
Bovine
(1)
Chinese hamster
(1)
Invertebrate
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 56. Show All 56 Publications.
Publications using NB100-56874 Applications Species
Asano S, Taniguchi Y, Yamawaki Y et al. Suppression of cell migration by phospholipase C-related catalytically inactive protein-dependent modulation of PI3K signalling Sci Rep 2017 Jul 14 [PMID: 28710365] (Human) Human
Ganta KK, Mandal A, Debnath S et al. Anti-HCV Activity from Semi-purified Methanolic Root Extracts of Valeriana wallichii Phytother Res Jan 12 2017 [PMID: 28078810]
Yamashita T, Aizawa H, Teramoto S et al. Calpain-dependent disruption of nucleo-cytoplasmic transport in ALS motor neurons Sci Rep. Jan 3 2017 [PMID: 28045133] (WB, Mouse) WB Mouse
Sasaki A, Hiwatashi K, Kumagai M et al. Relationships between the expression of hepatocyte nuclear factors and factors essential for lipoprotein production in a human mesenchymal stem cell line, UE7T-13. Biosci. Biotechnol. Biochem. Nov 14 2016 [PMID: 27838959] (WB, Human) WB Human
Ganta KK, Mandal A, Chaubey B, Depolarization of mitochondrial membrane potential is the initial event in non-nucleoside reverse transcriptase inhibitor efavirenz induced cytotoxicity Cell Biol. Toxicol. Sep 17 2016 [PMID: 27639578] (WB, Human) WB Human
Huang X, Huang R, Gou S et al. Combretastatin A-4 Analogue: A Dual-Targeting and Tubulin Inhibitor Containing Antitumor Pt(IV) Moiety with a Unique Mode of Action Bioconjug. Chem. Aug 15 2016 [PMID: 27494235] (WB, Human) WB Human
Mandal A, Ganta KK, Chaubey B. Combinations of siRNAs against La Autoantigen with NS5B or hVAP-A Have Additive Effect on Inhibition of HCV Replication. Hepat Res Treat 2016 Jun 29 [PMID: 27446609] (WB) WB
Ulugol A, Topuz RD, Gunduz O et al. Changes in nociceptin/orphanin FQ levels in rat brain regions after acute and chronic cannabinoid treatment in conjunction with the development of antinociceptive tolerance Fundam Clin Pharmacol 2016 Jul 01 [PMID: 27371029] (WB, IHC) WB, IHC
Schneider JS, Anderson DW, Kidd SK et al. Sex-Dependent Effects of Lead and Prenatal Stress on Post-translational Histone Modifications in Frontal Cortex and Hippocampus in the Early Postnatal Brain. Neurotoxicology. 2016 Mar 24 [PMID: 27018513] (WB, Mouse) WB Mouse
Nonaka A, Yoshida M, Iha M et al. Exogenous expression of homeoprotein EGAM1N prevents in vitro cardiomyogenesis by impairing expression of T and Nkx2.5, but not Mef2c, in mouse embryonic stem cells. Cytotechnology. 2016 Mar 17 [PMID: 26983934] (WB, Mouse) WB Mouse
Show All 56 Publications.

Reviews for beta-Actin Antibody (NB100-56874) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Feline.
We have 3 reviews tested in 1 application: WB.

Reviews using NB100-56874:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(1)
Feline
(1)
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Images Ratings Applications Species Date Details
Western Blot beta-Actin NB100-56874
Enlarge
reviewed by:
Kazuya Oikawa
WB Feline 05/18/2017
View

Summary

ApplicationWestern Blot
Sample TestedKidney tissue homogenates
SpeciesFeline
Lot12221897B-4
 
reviewed by:
YONGJIE LAI
WB 09/15/2014
View

Summary

ApplicationWestern Blot
Western Blot beta-Actin NB100-56874
Enlarge
reviewed by:
Anonymous
WB Human 08/19/2014
View

Summary

ApplicationWestern Blot
Sample TestedLysate from 293T cells
SpeciesHuman

Blocking

Blocking DetailsNon-fat dry milk (5%) in 1X PBS

Primary Anitbody

Dilution Ratio1:2000 dilution, 3 hrs at RT in 5% non-fat dry milk

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG-HRP conjugated antibody
Secondary Manufacturer Cat#65-6120
Secondary Concentration1:10000

Details

Detection NotesSuperSignal West Pico Chemiluminescent Substrate (Thermo), 10sec exposure using X-ray film

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Actin Antibody (NB100-56874). (Showing 1 - 6 of 6 FAQs).

  1. Why is beta actin used as a loading control in blotting ?
    • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
  2. Are the beta-Actin antibodies validated in Simple Western?
    • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
  3. Do Beta-Actin antibodies come in lyophilized format?
    • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
  4. What the theoretical molecular weight for Beta-Actin antibodies?
    • The TMW of beta Actin antibodies is approximately 40 - 42 kDa.
  5. I would like to find the manual description of this product, to confirm what dilution is recommended for Western blot.
    • The recommended starting dilution for Western blotting is 0.25-1ug/ml, but we suggest that you optimise this for your particular sample set.
  6. What is the immunogen sequence of this Beta-Actin antibody?
    • A sequence of amino acids 2 - 16 (CDDDIAALVIDNGSG) of beta Actin.

Secondary Antibodies

 

Isotype Controls

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Blogs on beta-Actin.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

Beta-Actin's Role in Neuronal Plasticity
Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading contro...  Read full blog post.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Kazuya Oikawa
05/18/2017
Application: WB
Species: Feline

 
YONGJIE LAI
09/15/2014
Application: WB
Species:

Anonymous
08/19/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol ACTB
Entrez