beta-Actin Antibody

Simple Western: beta-Actin Antibody [NB100-56874] - Image shows a specific band for Beta Actin in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: beta-Actin Antibody [NB100-56874] - Specific bands are seen in various cell lysates at the same molecular weight. A: human brain, B: mouse brain, C: rat brain, D: human lung, and E: human spleen tissue probed using beta actin antibody at 0.25 ug/mL.

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody [NB100-56874] - Actin was detected in NIH-3T3 cells fixed with methanol using rabbit anti-mouse Actin antibody (NB100-56874) at 1:100 dilution, overnight at 4C. Cells were stained using Northern Lights 557 conjugated anti-rabbit IgG secondary antibodies (NL004) and counterstained with DAPI.

Immunohistochemistry-Paraffin: beta-Actin Antibody [NB100-56874] - Analysis of human spleen using antibody at 10 ug/mL.

Western Blot: beta-Actin Antibody [NB100-56874] - Decreasing amounts of human ovary tissue lysate were probed using beta actin antibody at 0.25 ug/mL: A) 40 ug, B) 30 ug, C) 20 ug, D) 10 ug per lane.

• Reactivity: Hu, Mu, Rt, Po, Bv, ChHa, In
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

beta-Actin Antibody Summary

• Immunogen: Amino acids 2-16 (CDDDIAALVIDNGSG) of actin protein were used as the immunogen sequence for this beta-Actin Antibody.
• Specificity: The NCBI accession number for actin is AAA51567.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACTB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25 - 1 ug/mL
  • Simple Western 1:12.5
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/mL
  • Knockdown Validated
• Application Notes: This antibody is not suitable for testing heart or muscle lysate. Use in Immunohistochemistry reported in scientific literature (PMID 27371029). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Knockdown validation (PMID: 31835509).
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  beta-Actin Knockout 293T Cell Lysate

Reactivity Notes

Invertebrate, Porcine, Bovine, and Chinese Hamster reactivity reported in scientific literature (PMID: 25240497, 26593451, 18650284, and 19291423 respectively).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for beta-Actin Antibody

• ACTB
• actin, beta
• actin, cytoplasmic 1
• B-Actin
• Beta Actin
• beta cytoskeletal actin
• betaActin
• beta-Actin
• BRWS1
• I(2)-Actin
• PS1TP5-binding protein 1
• PS1TP5BP1

Background

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References

1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-Actin Antibody (NB100-56874)(71)

Publications using NB100-56874

• Sato S, Kikuchi T, Nishimura Y et al. Generation of mouse iPS cells using an inducible expression of transgenes via the cumate gene-switch Anal. Biochem. Apr 22 2020 [PMID: 32333903] (WB, Mouse)
• Wang L, Zhang S, Zhang W et Al. miR-424 Promotes Bovine Adipogenesis Through an Unconventional Post-Transcriptional Regulation of STK11 Front Genet Mar 4 2020 [PMID: 32194625] (WB, Bovine)
• Wang L, Zhang S, Cheng G et al. MiR-145 reduces the activity of PI3K/Akt and MAPK signaling pathways and inhibits adipogenesis in bovine preadipocytes Genomics Mar 3 2020 [PMID: 32135297] (WB)
• Uemasu K, Tanabe N, Tanimura K et Al. Serine Protease Imbalance in the Small Airways and Development of Centrilobular Emphysema in COPD Am. J. Respir. Cell Mol. Biol. Feb 26 2020 [PMID: 32101459] (Mouse)
• Su X, Wang Y, Li A Neudesin Neurotrophic Factor Promotes Bovine Preadipocyte Differentiation and Inhibits Myoblast Myogenesis Animals (Basel) Dec 10 2019 [PMID: 31835509] (WB, KD, Bovine)
• Ghezzi C, Wong A, Chen BY et al. A high-throughput screen identifies that CDK7 activates glucose consumption in lung cancer cells Nat Commun Nov 29 2019 [PMID: 31784510] (WB, Human)
• Huang YM, Cheng CH, Pan SL et al. Gene Expression Signature-Based Approach Identifies Antifungal Drug Ciclopirox As a Novel Inhibitor of HMGA2 in Colorectal Cancer Biomolecules Nov 2 2019 [PMID: 31684108] (WB, Human)
• Asano, S;Ikura, Y;Nishimoto, M;Yamawaki, Y;Hamao, K;Kamijo, K;Hirata, M;Kanematsu, T; Phospholipase C-related catalytically inactive protein regulates cytokinesis by protecting phosphatidylinositol 4,5-bisphosphate from metabolism in the cleavage furrow Sci Rep Sep 4 2019 [PMID: 31484968] (WB, Human)
• Tomita K, Fukumoto M, Itoh K et al. MiR-7-5p is a key factor that controls radioresistance via intracellular Fe2+ content in clinically relevant radioresistant cells Biochem. Biophys. Res. Commun. Aug 28 2019 [PMID: 31472959] (WB, Human)
• Bimonte, S;Cascella, M;Barbieri, A;Arra, C;Cuomo, A; Shining a Light on the Effects of the Combination of (-)-Epigallocatechin-3-gallate and Tapentadol on the Growth of Human Triple-negative Breast Cancer Cells In Vivo Jun 24 2019 [PMID: 31471393] (Human)
• Tomita K, Takashi Y, Ouchi Y et al. Lipid peroxidation increases hydrogen peroxide permeability leading to cell death in cancer cell lines that lack mtDNA Cancer Sci. Sep 1 2019 [PMID: 31314163] (WB, Human)
• Amersfoort J, Douna H, Schaftenaar FH et al. Defective Autophagy in T Cells Impairs the Development of Diet-Induced Hepatic Steatosis and Atherosclerosis Front Immunol Dec 12 2018 [PMID: 30619297] (WB, Mouse)
• Mandal A, Chaubey B. Expression of Duplex shRNAs through a Lentiviral Vector against Cellular and Viral Genes Inflicts Sustained Inhibition of Hepatitis C Virus Replication. Intervirology Sep 25 2018 [PMID: 30253401] (WB, Human)
• Adamiak M, Bujko K, Cymer M et al. Novel evidence that extracellular nucleotides and purinergic signaling induce innate immunity-mediated mobilization of hematopoietic stem/progenitor cells Leukemia 2018 Mar 30 [PMID: 29725032] (WB, Mouse)
• Dutta SM, Mustafi SB, Raha S, Chakraborty SK et al. Biomonitoring role of some cellular markers during heat stress-induced changes in highly representative fresh water mollusc, Bellamya bengalensis: Implication in climate change and biological adaptation Ecotoxicol. Environ. Saf. 2017 Aug 15 [PMID: 29655850] (WB, Invertebrate)
  
  Details:
  Bellamya bengalensis was used as part of this study.
• Asano S, Taniguchi Y, Yamawaki Y et al. Suppression of cell migration by phospholipase C-related catalytically inactive protein-dependent modulation of PI3K signalling Sci Rep 2017 Jul 14 [PMID: 28710365] (Human)
• Ganta KK, Mandal A, Debnath S et al. Anti-HCV Activity from Semi-purified Methanolic Root Extracts of Valeriana wallichii Phytother Res Jan 12 2017 [PMID: 28078810]
• Yamashita T, Aizawa H, Teramoto S et al. Calpain-dependent disruption of nucleo-cytoplasmic transport in ALS motor neurons Sci Rep. Jan 3 2017 [PMID: 28045133] (WB, Mouse)
• Sasaki A, Hiwatashi K, Kumagai M et al. Relationships between the expression of hepatocyte nuclear factors and factors essential for lipoprotein production in a human mesenchymal stem cell line, UE7T-13. Biosci. Biotechnol. Biochem. Nov 14 2016 [PMID: 27838959] (WB, Human)
• Ganta KK, Mandal A, Chaubey B, Depolarization of mitochondrial membrane potential is the initial event in non-nucleoside reverse transcriptase inhibitor efavirenz induced cytotoxicity Cell Biol. Toxicol. Sep 17 2016 [PMID: 27639578] (WB, Human)
• Huang X, Huang R, Gou S et al. Combretastatin A-4 Analogue: A Dual-Targeting and Tubulin Inhibitor Containing Antitumor Pt(IV) Moiety with a Unique Mode of Action Bioconjug. Chem. Aug 15 2016 [PMID: 27494235] (WB, Human)
• Mandal A, Ganta KK, Chaubey B. Combinations of siRNAs against La Autoantigen with NS5B or hVAP-A Have Additive Effect on Inhibition of HCV Replication. Hepat Res Treat 2016 Jun 29 [PMID: 27446609] (WB)
• Ulugol A, Topuz RD, Gunduz O et al. Changes in nociceptin/orphanin FQ levels in rat brain regions after acute and chronic cannabinoid treatment in conjunction with the development of antinociceptive tolerance Fundam Clin Pharmacol 2016 Jul 01 [PMID: 27371029] (WB, IHC)
• Schneider JS, Anderson DW, Kidd SK et al. Sex-Dependent Effects of Lead and Prenatal Stress on Post-translational Histone Modifications in Frontal Cortex and Hippocampus in the Early Postnatal Brain. Neurotoxicology. 2016 Mar 24 [PMID: 27018513] (WB, Mouse)
• Nonaka A, Yoshida M, Iha M et al. Exogenous expression of homeoprotein EGAM1N prevents in vitro cardiomyogenesis by impairing expression of T and Nkx2.5, but not Mef2c, in mouse embryonic stem cells. Cytotechnology. 2016 Mar 17 [PMID: 26983934] (WB, Mouse)
• Lu A, Pallero MA, Lei W et al. Inhibition of Transforming Growth Factor beta Activation Diminishes Tumor Progression and Osteolytic Bone Disease in Mouse Models of Multiple Myeloma. Am. J. Pathol. 2016 Jan 19 [PMID: 26801735] (WB, Mouse)
• Yamashita T, Teramoto S, Kwak S. Phosphorylated TDP-43 becomes resistant to cleavage by calpain: A regulatory role for phosphorylation in TDP-43 pathology of ALS/FTLD. Neurosci. Res. 2015 Dec 23 [PMID: 26723245] (WB, Human)
• Oue K, Zhang J, Harada-Hada K et al. Phospholipase C-related catalytically inactive protein is a new modulator of thermogenesis promoted by beta-adrenergic receptors in brown adipocytes. J. Biol. Chem. 2015 Dec 25 [PMID: 26706316] (WB, Mouse)
• Li Y, Tanaka K, Wang L et al. Induction of Memory Deficit in Mice with Chronic Exposure to Cerebrospinal Fluid from Patients with Anti-N-Methyl-D-Aspartate Receptor Encephalitis. Tohoku J. Exp. Med. 2015 Nov 30 [PMID: 26616272] (WB, Mouse)
• Goasdoue K, Awabdy D, Bjorkman ST, Miller S. Standard loading controls are not reliable for western blot quantification across brain development or in pathological conditions. Electrophoresis. 2015 Nov 23 [PMID: 26593451] (WB, Porcine)
• Bakey Z, Bihoreau MT, Piedagnel R et al. The SAM domain of ANKS6 has different interacting partners and mutations can induce different cystic phenotypes Kidney Int. 2015 Jun 03 [PMID: 26039630] (WB, Rat, Mouse)
• Sasaki S, Yamashita T, Shin K. Autophagy in spinal motor neurons of conditional ADAR2-knockout mice: An implication for a role of calcium in increased autophagy flux in ALS. Neurosci. Lett. 2015 May 14 [PMID: 25980994] (WB, Mouse)
• Mahata B, Banerjee A, Kundu M et al. TALEN mediated targeted editing of GM2/GD2-synthase gene modulates anchorage independent growth by reducing anoikis resistance in mouse tumor cells Sci Rep 2015 Mar 12 [PMID: 25762467] (WB, Mouse)
• El Machhour F, Keuylian Z, Kavvadas P et al. Activation of Notch3 in Glomeruli Promotes the Development of Rapidly Progressive Renal Disease. J. Am. Soc. Nephrol. 2014 Nov 24 [PMID: 25421577] (WB, Mouse)
• Dutta SM, Mustafi SB, Raha S, Chakraborty SK. Assessment of thermal stress adaptation by monitoring Hsp70 and MnSOD in the freshwater gastropod, Bellamya bengalensis (Lamark 1882). Environ Monit Assess. 2014 Sep 21 [PMID: 25240497] (WB, Invertebrate)
  
  Details:
  Beta actin antibody used for WB on freshwater gastropod/snail Bellamya bengalensis (Lamark 1882) specimens collected during summer and winter seasons from two contrasting wetlands - an ecorestored /free from human interference site SI and the other experiencing anthropogenic stresses/SII (Fig. 3)
• Chao Yinxia, Kaliaperumal Nivashini, Chretien Anne-Sophie et al. Human plasmacytoid dendritic cells regulate IFN-alpha production through activation-induced splicing of IL-18Ralpha. J Leukoc Biol. 2014 Dec [PMID: 25170117]
• Loov C, Nadadhur AG, Hillered L et al. Extracellular Ezrin - a Novel Biomarker for Traumatic Brain Injury. J Neurotrauma. 2014 Aug 02 [PMID: 25087457] (WB, Rat, Human)
  
  Details:
  Beta actin antibody used for WB on studies involving establishment of extracellular Ezrin as a novel biomarker for traumatic brain injury in human and rat models.
• Kumar A, Sahu SK, Mohanty S et al. Kaposi Sarcoma Herpes Virus Latency Associated Nuclear Antigen Protein Release the G2/M Cell Cycle Blocks by Modulating ATM/ATR Mediated Checkpoint Pathway. PLoS ONE. 2014 Jun 28 [PMID: 24972086] (WB, Human)
  
  Details:
  Fig 4A: BJAB B lymphoma cells. The beta actin pAb was used as a control to standardize protein loading.
• Okumura T, Harada K, Oue K et al. Phospholipase C-Related Catalytically Inactive Protein (PRIP) Regulates Lipolysis in Adipose Tissue by Modulating the Phosphorylation of Hormone-Sensitive Lipase. PLoS ONE. 2014 Jun 20 [PMID: 24945349] (WB, Mouse)
  
  Details:
  Figs 2,3, 4, 6: Epididymal white adipose tissue. The beta actin pAb was used as a cytosolic marker in protein fractions.
• Morita K, Shiraishi S, Motoyama N et al. Palliation of bone cancer pain by antagonists of platelet-activating factor receptors. PLoS ONE 2014 Mar 18 [PMID: 24637403] (WB, Mouse)
  
  Details:
  Mouse spinal cord, Fig 3. The Beta Actin antibody was used as a loading control to standardize protein.
• Sugawara S, Ito T, Sato S et al. Production of an aminoterminally truncated, stable type of bioactive mouse fibroblast growth factor 4 in Escherichia coli. J Biosci Bioeng 2013 Nov 6 [PMID: 24210555] (WB, Mouse)
• Kamal A, Tamboli JR, Ramaiah MJ et al. Quinazolino linked 4beta-amidopodophyllotoxin conjugates regulate angiogenic pathway and control breast cancer cell proliferation. Bioorg Med Chem 2013 Nov 1 [PMID: 24055291] (WB, Human)
  
  Details:
  MCF-7 human breast carcinoma cells, Fig 4C. Beta actin was detected at 42 kDa. Beta-actin was used as a protein loading control.
• Kumar A, Kant S, Singh SM. Antitumor and chemosensitizing action of dichloroacetate implicates modulation of tumor microenvironment: A role of reorganized glucose metabolism, cell survival regulation and macrophage differentiation. Toxicol Appl Pharmacol 2013 Nov 15 [PMID: 24051182] (WB, Mouse)
  
  Details:
  Mouse Dalton's lymphoma, Figs 2B, 3C, 4B, 6F;Beta actin was used as a protein loading WB control.
• Kumar A, Kant S, Singh SM. Targeting monocarboxylate transporter by alpha-cyano-4-hydroxycinnamate modulates apoptosis and cisplatin resistance of Colo205 cells: implication of altered cell survival regulation. Apoptosis 2013 Dec [PMID: 23955790] (WB, Human)
• Sivadas VP, George NA, Kattoor J, Kannan S. Novel mutations and expression alterations in SMAD3/TGFBR2 genes in oral carcinoma correlate with poor prognosis. Genes Chromosomes Cancer 2013 Nov [PMID: 23913824] (WB, Human)
• Yoshii Yukie, Furukawa Takako, Oyama Nobuyuki et al. Fatty acid synthase is a key target in multiple essential tumor functions of prostate cancer: uptake of radiolabeled acetate as a predictor of the targeted therapy outcome. PLoS One. 2013 [PMID: 23741342] (WB)
• Zimmerman KA, Graham LV, Pallero MA et al. Calreticulin regulates transforming growth factor-B-stimulated extracellular matrix production. J Biol Chem. 2013 May 17 [PMID: 23564462]
• Sato Sho, Morita Sanae, Iha Momoe et al. Intact structure of EGAM1 homeoproteins and basic amino acid residues in the common homeodomain of EGAM1 and EGAM1C contribute to their nuclear localization in mouse embryonic stem cells. J Biosci Bioeng. 2013 Aug [PMID: 23510645] (Mouse)
• Saha T. LAMP2A overexpression in breast tumors promotes cancer cell survival via chaperone-mediated autophagy. Autophagy. 2012 Nov [PMID: 22874552]
• Iha M, Soma M, Sato S et al. Severe inhibition of in vitro cardiomyogenesis in mouse embryonic stem cells ectopically expressing EGAM1C homeoprotein. Biosci Biotechnol Biochem. 2012 [PMID: 22785471]
• Soma M, Iha M, Kihara Y et al. Preferential emergence of cell types expressing markers for primitive endoderm lineages in mouse embryonic stem cells expressing exogenous EGAM1 homeoprotein. J Biosci Bioeng. 2012 Sep [PMID: 22608553] (WB, Mouse)
  
  Details:
  Beta Actin (IMG-5142A) WB: Mouse embryonic stem cells (MG1.19), Fig 1A.
• Vishvakarma NK, Kumar A, Singh V et al. Hyperglycemia of tumor microenvironment modulates stage-dependent tumor progression and multidrug resistance: implication of cell survival regulatory molecules and altered glucose transport. Mol Carcinog 2013 Dec [PMID: 22566271] (WB, Mouse)
• Iha M, Watanabe M, Kihara Y et al. Effect of ectopic expression of homeoprotein EGAM1C on the cell morphology, growth, and differentiation in a mouse embryonic stem cell line, MG1.19 cells. Reproduction. 2012 Apr [PMID: 22367590]
• Sato M, Uchida K, Nakajima H et al. Direct transplantation of mesenchymal stem cells into the knee joints of Hartley strain guinea pigs with spontaneous osteoarthritis. Arthritis Res Ther. 2012 Feb 7 [PMID: 22314040]
• Gerstner JR, Vanderheyden WM, LaVaute T et al. Time of day regulates subcellular trafficking, tripartite synaptic localization, and polyadenylation of the astrocytic Fabp7 mRNA. J Neurosci. 2012 Jan 25 [PMID: 22279223] (WB, Mouse)
  
  Details:
  GADPH(IMG-3970) & Beta-Actin(IMG-5142A). WB: Mouse brain cells: Fig1A (IMG-5142A). WB: Mouse brain cells, Fig 4A (IMG-3970).
• Sun P, Wang F, Wang L et al. Increase in cortical pyramidal cell excitability accompanies depression-like behavior in mice: a transcranial magnetic stimulation study. J Neurosci. 2011 Nov 9 [PMID: 22072696] (WB, Mouse)
  
  Details:
  Beta-actin (IMG-5142A). WB: Cingulate cortex mouse brain cells, Figs 6A & 6B.
• Kamitani Shigeki, Ao Shinpei, Toshima Hirono et al. Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibodies recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq. FEBS J. 2011 Aug [PMID: 21624053]
• Ishiguro Hiroki, Horiuchi Yasue, Ishikawa Maya et al. Brain cannabinoid CB2 receptor in schizophrenia. Biol Psychiatry. 2010 May 15 [PMID: 19931854]
• Acharya S, Dilnawaz F, Sahoo SK. Targeted epidermal growth factor receptor nanoparticle bioconjugates for breast cancer therapy. Biomaterials. 2009 Oct [PMID: 19631377] (WB)
  
  Details:
  Products cited: 1. EGFR (IMG-80179) FA: The EGFR antibody was conjugated to 6-coumarin loaded nanoparticles. The EGFR antibody was labeled with a FITC secondary antibody (Cat#(2010)2). Intracellular uptake of the antibody labeled nanoparticles in MCF 7 br
• Sahoo DK, Ranjan R, Kumar D et al. An alternative method of promoter assessment by confocal laser scanning microscopy. J Virol Methods. 2009 Oct [PMID: 19540268] (WB)
  
  Details:
  Product cited: 1. GFP (IMG-5126A): WB, transformed Xanthi Brad protoplasts with different plasmid constructs (see paper). 2. Actin (IMG-5142A) WB, used as loading control.
• Pohjanvirta Raimo. Transgenic mouse lines expressing rat AH receptor variants--a new animal model for research on AH receptor function and dioxin toxicity mechanisms. Toxicol Appl Pharmacol. 2009 Apr 15 [PMID: 19371626]
• Banerjee Mustafi S, Chakraborty PK, Dey RS, Raha S. Heat stress upregulates chaperone heat shock protein 70 and antioxidant manganese superoxide dismutase through reactive oxygen species (ROS), p38MAPK, and Akt. Cell Stress Chaperones. 2009 Nov [PMID: 19291423] (WB, Chinese hamster)
  
  Details:
  beta Actin (IMG-5142A). WB: Heat treated Chinese hamster lung fibroblasts (V79), Fig 1 & 2.
• Huang J, Ren T, Guan H et al. HTLV-1 Tax is a critical lipid raft modulator that hijacks IkappaB kinases to the microdomains for persistent activation of NF-kappaB. J Biol Chem. 2009 Mar 6 [PMID: 19129196] (WB, Human)
  
  Details:
  WB (human T cell lines), Fig. 1B.
• Karcher EL, Bayles DO, Bannantine JP et al. Osteopontin: a novel cytokine involved in the regulation of Mycobacterium avium subspecies paratuberculosis infection in periparturient dairy cattle. J Dairy Sci. 2008 Aug [PMID: 18650284] (WB, Bovine)
  
  Details:
  WB (cow PBMCs), Fig. 9A.
• Morita K, Motoyama N, Kitayama T et al. Spinal antiallodynia action of glycine transporter inhibitors in neuropathic pain models in mice. J Pharmacol Exp Ther. 2008 Aug [PMID: 18448867] (WB, Mouse)
  
  Details:
  WB (mouse spinal cord tissue lysates), Fig. 2A,B.
• Gerstner JR, Bremer QZ, Vander Heyden WM et al. Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain. PLoS One. 2008 Feb 20 [PMID: 18286188]
• He Z, Jiang J, Kokkinaki M et al. Gdnf upregulates c-Fos transcription via the Ras/Erk1/2 pathway to promote mouse spermatogonial stem cell proliferation. Stem Cells. 2008 Jan [PMID: 17962702]
• He Z, Jiang J, Hofmann MC, Dym M. Gfra1 silencing in mouse spermatogonial stem cells results in their differentiation via the inactivation of RET tyrosine kinase. Biol Reprod. 2007 Oct [PMID: 17625109] (WB, Mouse)
  
  Details:
  WB (mouse sperm): 1. GFRA1 (IMG-192), Figs 1B, 5D (siRNA validated).
• Acosta GHG. Susceptibility of parkinson's disease following mild blast traumatic brain injury Thesis. 2014 Aug 01 (WB, Rat)
• Kawaguchi Yoshifumi, Miyamoto Yasuhide, Inoue Takehiro, Kaneda Yasufumi. Efficient eradication of hormone-resistant human prostate cancers by inactivated Sendai virus particle. Int J Cancer. 2009 May 15 [PMID: 19173282]
• Yoshimura R. Study of branched chain amino acids as physiologically active substances Thesis 2016 (WB, Rat)

Reviews for beta-Actin Antibody (NB100-56874) (4)

Average Rating: 4

(Based on 4 reviews)

We have 4 reviews tested in 3 species: Human, Feline, human.

reviewed by: Anonymous Simple Western human 03/20/2020

Summary

• Application: Simple Western
• Sample Tested: liver
• Species: human
• Lot: 12221897B-7

reviewed by: Kazuya Oikawa WB Feline 05/18/2017

Summary

• Application: Western Blot
• Sample Tested: Kidney tissue homogenates
• Species: Feline
• Lot: 12221897B-4

reviewed by: YONGJIE LAI WB 09/15/2014

Summary

• Application: Western Blot

reviewed by: Anonymous WB Human 08/19/2014

Summary

• Application: Western Blot
• Sample Tested: Lysate from 293T cells
• Species: Human

Blocking

• Blocking Details: Non-fat dry milk (5%) in 1X PBS

Primary Anitbody

• Dilution Ratio: 1:2000 dilution, 3 hrs at RT in 5% non-fat dry milk

Secondary Antibody

• Secondary Description: Anti-rabbit IgG-HRP conjugated antibody
• Secondary Manufacturer Cat#: 65-6120
• Secondary Concentration: 1:10000

Details

• Detection Notes: SuperSignal West Pico Chemiluminescent Substrate (Thermo), 10sec exposure using X-ray film

FAQs for beta-Actin Antibody (NB100-56874). (Showing 1 - 7 of 7 FAQs).

1. Why is beta actin used as a loading control in blotting ?
   • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
2. Are the beta-Actin antibodies validated in Simple Western?
   • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
3. Do Beta-Actin antibodies come in lyophilized format?
   • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
4. What is the theoretical molecular weight for Beta-Actin antibodies?
   • The TMW for beta Actin antibodies is approximately 42 kDa.
5. I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
   • For homogenized tissue, beta-actin and GAPDH are both fine.
6. I would like to find the manual description of this product, to confirm what dilution is recommended for Western blot.
   • The recommended starting dilution for Western blotting is 0.25-1ug/ml, but we suggest that you optimise this for your particular sample set.
7. What is the immunogen sequence of this Beta-Actin antibody?
   • A sequence of amino acids 2 - 16 (CDDDIAALVIDNGSG) of beta Actin.

Bioinformatics

• Gene Symbol: ACTB
• Entrez:
  • 60(Human)
  • 11461(Mouse)
  • 81822(Rat)