AIF-1/Iba1 Antibody

Images

 
Western Blot: AIF-1/Iba1 Antibody [NBP2-19019] - Various 50 ug tissue extracts (Molecular weight: 16.7 KDa) were separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 Antibody diluted at 1:1000. The ...read more
Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: AIF-1/Iba1 stained by AIF-1/Iba1 Antibody diluted at 1:500. Blue: Hoechst ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Rat cerebellum stained by AIF-1/Iba1 Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-19019] - THP-1 cell. Black: Unlabelled sample was used as a control.Red: AIF-1:Iba1 Antibody dilution: 1:50.
Western Blot: AIF-1/Iba1 Antibody [NBP2-19019] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 Antibody diluted at 1:1000.
Western Blot: AIF-1/Iba1 Antibody [NBP2-19019] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 Antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] - AIF-1/Iba1 Antibody (1 1:1000, Red) expression in dorsal root ganglia. Green fluorescent Nissl was used as counter-staining. Image submitted by ...read more
Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-19019] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AIF-1/Iba1 protein stained by AIF-1/Iba1 Antibody diluted at 1:400. ...read more
Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NBP2-19019] - Frozen sectioned E13.5 Rat brain. Green: AIF-1/Iba1 protein stained by AIF-1/Iba1 Antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Analysis of paraffin-embedded human hepatoma, using AIF-1/Iba1 Antibody at 1:500 dilution.Iba1 antibody detects AIF-1/Iba1 in the cytosol of Kupffer ...read more
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Mouse cerebellum stained by AIF-1/Iba1 Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Paraffin-embedded mouse fore brain stained with AIF-1/Iba1 Antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Paraffin-embedded rat hind brain AIF-1/Iba1 Antibody at a dilution of 1:500.
Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-19019] - Rat brain stained by AIF-1/Iba1 Antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Po, RMSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

AIF-1/Iba1 Antibody Summary

Immunogen
This AIF-1/Iba1 Antibody was developed against a carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.
Marker
pan-Microglia Marker
Predicted Species
Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AIF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
Application Notes
AIF-1/Iba1 antibody validated for ICC/IF from a verified customer review.
Theoretical MW
16.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 3.8
using
NBP2-19019 in the following applications:

Publications
Read Publications using
NBP2-19019 in the following applications:

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 30657788).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 1% BSA, 20% Glycerol (pH7)
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for AIF-1/Iba1 Antibody

  • AIF1
  • AIF-1
  • allograft inflammatory factor 1
  • G1
  • IBA1
  • IbaI
  • interferon gamma responsive transcript
  • Ionized calcium-binding adapter molecule 1
  • IRT1
  • IRT-1
  • Protein G1

Background

Allograft inflammatory factor (AIF-1) or ionized calcium-binding adapter molecule 1 (Iba1) is a cytosolic actin binding protein containing a calcium binding domain (EF-hand) and inducible by cytokines and INF-gamma (1). AIF-1 (theoretical molecular weight 17kDa) was first cloned from rat and human cardiac allografts and macrophage cell lines. In cardiac allografts, expression of AIF-1 was specifically associated with infiltrated mononuclear cells (2, 3). AIF-1 is constitutively expressed and inducible in macrophages and microglia. Iba1, microglia response factor (MRF-1) and daintain were independently cloned and identified in rat and human tissues and share complete sequence identity with AIF-1. Additionally, several AIF-1 splice variants have been identified including IRT-1, BART-1, G1 and Hara-1 (2).

Several cellular functions have been associated with AIF-1/Iba1 expression including cell growth, cell migration, actin bundling, membrane ruffling, and phagocytic activity (2, 4). Iba1 induces Rac signaling through a PLC-gamma dependent pathway (1). Rac, a member of the Rho family of small GTPases, localizes with Iba1 and F-actin in membrane ruffles and phagocytic cups and plays a role in microglia activation. AIF-1/Iba1 induction in macrophages and microglia occur in association with immunological inflammatory processes in various disease states including endometriosis, cerebral infarction and rheumatoid arthritis (5). Immunodetection of Iba1 through flow cytometry, immunohistochemical or immunocytochemical applications is commonly used for identification and analysis of microglia.

References

1. Imai, Y., & Kohsaka, S. (2002). Intracellular signaling in M-CSF-induced microglia activation: Role of Iba1. GLIA. https://doi.org/10.1002/glia.10149

2. Deininger, M. H., Meyermann, R., & Schluesener, H. J. (2002). The allograft inflammatory factor-1 family of proteins. FEBS Letters. https://doi.org/10.1016/S0014-5793(02)02430-4

3. Utans, U., Quist, W. C., Mcmanus, B. M., Wilson, J. E., Arceci, R. J., Wallace, A. F., & Russell, M. E. (1996). Allograft inflammatory factor-1: A cytokine-responsive macrophage molecule expressed in transplanted human hearts. Transplantation. https://doi.org/10.1097/00007890-199605150-00018

4. Franco-Bocanegra, McAuley, Nicoll, & Boche. (2019). Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer's Disease. Cells. https://doi.org/10.3390/cells8060639

5. Kimura, M., Kawahito, Y., Obayashi, H., Ohta, M., Hara, H., Adachi, T., ... Yoshikawa, T. (2007). A Critical Role for Allograft Inflammatory Factor-1 in the Pathogenesis of Rheumatoid Arthritis. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.5.3316

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AIF-1/Iba1 Antibody (NBP2-19019)(13)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Monkey, Porcine.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(3)
IHC-Fr
(3)
IHC-P
(1)
WB
(4)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Rat
(4)
Monkey
(1)
Porcine
(1)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NBP2-19019 Applications Species
Chamera K, Trojan E, Kotarska K et al. Role of Polyinosinic:Polycytidylic Acid-Induced Maternal Immune Activation and Subsequent Immune Challenge in the Behaviour and Microglial Cell Trajectory in Adult Offspring: A Study of the Neurodevelopmental Model of Schizophrenia International journal of molecular sciences Feb 4 2021 [PMID: 33557113] (WB, Rat) WB Rat
Kim J, Suh YH, Chang KA Interleukin-17 induced by cumulative mild stress promoted depression-like behaviors in young adult mice Molecular brain Jan 13 2021 [PMID: 33441182] (IHC-Fr, Mouse) IHC-Fr Mouse
Liu L, Chen M, Lin K et al. Inhibiting Caspase-12 Mediated Inflammasome Activation protects against Oxygen-Glucose Deprivation Injury in Primary Astrocytes Int. J. Med. Sci. Jul 24 2020 [PMID: 32788872] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Chamera K, Szuster-Gluszczak M, Trojan E, Basta-Kaim A Maternal Immune Activation Sensitizes Male Offspring Rats to Lipopolysaccharide-Induced Microglial Deficits Involving the Dysfunction of CD200-CD200R and CX3CL1-CX3CR1 Systems Cells Jul 12 2020 [PMID: 32664639] (WB, Rat) WB Rat
Merlo S, Luaces JP, Spampinato SF et al. SIRT1 Mediates Melatonin's Effects on Microglial Activation in Hypoxia: In Vitro and In Vivo Evidence Biomolecules Feb 27 2020 [PMID: 32120833] (IHC, IHC-P, Rat) IHC, IHC-P Rat
Hirshman NA, Hughes FM, Jin H, et al. Cyclophosphamide-Induced Cystitis Results in NLRP3-Mediated Inflammation in the Hippocampus and Symptoms of Depression in Rats Am. J. Physiol. Renal Physiol. Dec 23 2019 [PMID: 31869244] (IHC, Rat) IHC Rat
Xu G, Yamamoto N, Nojima T, et al. Intravenously infusing the secretome of adipose-derived mesenchymal stem cells ameliorates neuroinflammation and neurological functioning after traumatic brain injury Stem Cells Dev. Dec 13 2019 [PMID: 31830866]
Han TU, Root J, Reyes LD et al. Human GNPTAB stuttering mutations engineered into mice cause vocalization deficits and astrocyte pathology in the corpus callosum Proc. Natl. Acad. Sci. U.S.A. Aug 27 2019 [PMID: 31405983] (IHC-Fr, Mouse) IHC-Fr Mouse
Gurung S, Reuter N, Preno A et al. Zika virus infection at mid-gestation results in fetal cerebral cortical injury and fetal death in the olive baboon PLoS Pathog. Jan 1 2019 [PMID: 30657788] (IHC-Fr, Monkey) IHC-Fr Monkey
Merlo S, Spampinato SF, Beneventano M, Sortino MA. The contribution of microglia to early synaptic compensatory responses that precede B-amyloid-induced neuronal death Sci Rep May 8 2018 [PMID: 29740062] (Human) Human
Show All 13 Publications.

Reviews for AIF-1/Iba1 Antibody (NBP2-19019) (4) 3.84

Average Rating: 3.8
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Mouse, Sheep
.

Reviews using NBP2-19019:
Filter by Applications
IHC-Fr
(1)
WB
(1)
ICC
(2)
All Applications
Filter by Species
Mouse
(3)
Sheep
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen AIF-1/Iba1 NBP2-19019
Enlarge
1
reviewed by:
Casey Nestor
IHC-Fr Sheep
 03/13/2020
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedBrain (hypothalamus) tissue
SpeciesSheep
Lot43678

Comments

Comments*Low Rating Note: Novus Innovators Program - new species or application used on a primary antibody:
To test, we used free-floating sheep hypothalamic brain sections from two animals. We did dilutions of 1:100 to 1:1000 and did not see any staining in our tissue. We also employed an antigen retrieval step in one of our runs, and it did not yield any different results.
Western Blot AIF-1/Iba1 NBP2-19019
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 12/18/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse forebrain lysate
SpeciesMouse
Lot39476
  5
reviewed by:
Verified Customer
ICC Mouse 04/01/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedC57BL/6 mouse retina
SpeciesMouse
LotN/A
Immunocytochemistry AIF-1/Iba1 NBP2-19019
Enlarge
4
reviewed by:
Temugin Berta
ICC Mouse 03/10/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedDorsal root ganglia
SpeciesMouse
Lot39476

Comments

CommentsBlocking and antibody solution: BSA1%/Triton 02% in PBS
Dilution AIF1: 1/1000
Secondary antibody: Alexa 555
Counter-staining: Nissl

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AIF-1/Iba1 Antibody (NBP2-19019). (Showing 1 - 1 of 1 FAQs).

  1. For  prepared antibody NBP2-19019, what is the corresponding  amino acid sequence?
    • The AIF-1/Iba1 antibody (NBP2-19019) was generated using a carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary, but we can share that it is within aa 97-147 (Isoform 1) - https://www.uniprot.org/uniprot/P55008).  The full-length human form for this one is 147aa total.Using the exact sequence, there is 100% identity to - https://www.uniprot.org/uniprot/Q5TM25

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Recent Reviews

3.8
5
2
4
1
3
0
2
0
1
1

Casey Nestor
03/13/2020
Application: IHC-Fr
Species: Sheep

Verified Customer
12/18/2017
Application: WB
Species: Mouse

 
Verified Customer
04/01/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol AIF1
Entrez