PCNA Antibody (PC10)

Images

 
Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - NIH3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at ...read more
Immunohistochemistry-Paraffin: PCNA Antibody (PC10) [NB500-106] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization.
Western Blot: PCNA Antibody (PC10) [NB500-106] - Analysis of human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE lysate) cell extracts.
Western Blot: PCNA Antibody (PC10) [NB500-106] - Western blot of PCNA levels across different stages of the cell cycle. U-2 OS cells were synchronised at G1/S with 2 mM thymidine an released for 10 hours for G2/M or ...read more
Immunohistochemistry-Frozen: PCNA Antibody (PC10) [NB500-106] - Paraformaldehyde fixed frozen section of brain from murine embryo using PCNA antibody clone PC10 (green), an IB4 antibody (red) and DAPI. Image provided by ...read more
Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - PC12 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at 1 ...read more
Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL ...read more
Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Development of the germinal zones in the tree shrew neocortex. Immunofluorescence for PCNA (yellow) and DAPI staining (blue) on 30 um-cryosections of E32-P1 tree ...read more
Flow Cytometry: PCNA Antibody (PC10) [NB500-106] - An intracellular stain was performed on HepG2 cells with PCNA Antibody (PC10) NB500-106G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more
Western Blot: PCNA Antibody (PC10) [NB500-106] - HeLa cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a ...read more
Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% ...read more
Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Dr, Fi, Mar, Pm, Rb, Ye, ZeSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
Clone
PC10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PCNA Antibody (PC10) Summary

Immunogen
Protein A-rat PCNA fusion obtained from pC2T
Marker
Proliferation Marker
Specificity
Specific for PCNA p36 protein expressed at high levels in proliferating cells.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PCNA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • ELISA
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:2000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Immunoprecipitation 1:100
  • Simple Western 1:200
  • Western Blot 1:2000
Application Notes
Use in Immunocytochemistry/Immunofluorescence has been reported in scientific literature (PMID: 22068968). It detects a band at 30 kDa by Western blot. For IHC on paraffin sections, heat-mediated citrate buffer antigen retrieval is recommended. ELISA was reported in scientific literature. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 26883631). Use in Immunohistochemistry-free floating reported in scientific literature (PMID: 27212918).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
PCNA Overexpression Lysate
Reviewed Applications
Read 5 Reviews rated 4.8
using
NB500-106 in the following applications:

Publications
Read Publications using
NB500-106 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33802807). Use in Marsupial reported in scientific literature (PMID:29253253).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for PCNA Antibody (PC10)

  • cyclin
  • DNA polymerase delta auxiliary protein
  • MGC8367
  • PCNA
  • proliferating cell nuclear antigen

Background

PCNA (Proliferating Cell Nuclear Antigen), plays a central role in coordinating the association of replication factors during DNA replication as well as during recognition and repair of DNA damage. PCNA forms a toroidal, ring-shaped structure of 90 kD by the symmetric association of three identical monomers. The ring encircles the DNA, acts as a platform upon which polymerases and other proteins dock to perform various DNA metabolic processes, and functions as a DNA polymerase-delta co-factor. The association of proteins with the replication fork through interactions with PCNA is regulated by several mechanisms. PCNA interacts with numerous proteins to facilitate DNA replication and repair, epigenetic processes and sister chromatid cohesion. PCNA has its highest expression during G1and S-phases, and its expression decreases in G2 and M-phases. This marker is also present in the early G0 phase because of its long half-live of 8-20 h. PCNA is a nuclear nonhistone protein that is necessary for DNA synthesis and is an accessory protein for DNA polymerase alpha, which is elevated during the G1/S phase of the cell cycle. Several studies have been performed to evaluate cell proliferation using PCNA and Ki-67 in different tumors of various origins; compared with PCNA, Ki-67 has been shown to be more sensitive and specific in the various tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB500-106
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Mar, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl

Publications for PCNA Antibody (NB500-106)(38)

We have publications tested in 11 confirmed species: Human, Mouse, Rat, Chicken, Fish, Marsupial, Porcine, Primate, Rabbit, Yeast, Zebrafish.

We have publications tested in 10 applications: ChIP, ELISA, FLOW, ICC/IF, IHC, IHC-Fr, IHC-FrFl, IHC-P, IP, WB.


Filter By Application
ChIP
(1)
ELISA
(1)
FLOW
(1)
ICC/IF
(3)
IHC
(9)
IHC-Fr
(1)
IHC-FrFl
(1)
IHC-P
(11)
IP
(1)
WB
(11)
All Applications
Filter By Species
Human
(8)
Mouse
(8)
Rat
(5)
Chicken
(1)
Fish
(4)
Marsupial
(1)
Porcine
(1)
Primate
(1)
Rabbit
(1)
Yeast
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using NB500-106 Applications Species
Martins RR, Zamzam M, Tracey-White D et al. MUller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina Aging cell Mar 22 2022 [PMID: 35315590] (IHC, Fish) IHC Fish
Bender H, Fietz Sa, Richter F, Stanojlovic M Alpha-Synuclein Pathology Coincides With Increased Number of Early Stage Neural Progenitors in the Adult Hippocampus Frontiers in cell and developmental biology Jul 7 2021 [PMID: 34307368] (IHC) IHC
Sakurai R, Singh H, Wang Y et al. Effect of Perinatal Vitamin D Deficiency on Lung Mesenchymal Stem Cell Differentiation and Injury Repair Potential American journal of respiratory cell and molecular biology Jun 14 2021 [PMID: 34126864]
Rossi G, Placidi M, Castellini C et al. Crocetin Mitigates Irradiation Injury in an In Vitro Model of the Pubertal Testis: Focus on Biological Effects and Molecular Mechanisms Molecules Mar 17 2021 [PMID: 33802807] (WB, Mouse) WB Mouse
Verboom L, Martens A, Priem D et al. OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis Cell Rep Feb 18 2020 [PMID: 32075762] (WB, Mouse) WB Mouse
Wong MK, Uchida M, Tsukada T Histological differentiation of mucus cell subtypes suggests functional compartmentation in the eel esophagus Cell Tissue Res. Jan 3 2020 [PMID: 31900664]
Yazdankhah M, Shang P, Ghosh S et al. Modulating EGFR-MTORC1-autophagy as a potential therapy for persistent fetal vasculature (PFV) disease Autophagy Sep 1 2019 [PMID: 31462148] (WB, Rat) WB Rat
Di Emidio G, Santini SJ, D'Alessandro AM et al. SIRT1 participates in the response to methylglyoxal-dependent glycative stress in mouse oocytes and ovary Biochim Biophys Acta Mol Basis Dis Feb 13 2019 [PMID: 30771486] (IHC-P, WB, Mouse) IHC-P, WB Mouse
Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods Jan 11 2018 [PMID: 30377371] (Human)

Details:
Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).
Human
Fukasawa H, Hashimoto H, Hiramoto k et al. Morphological characterization of small hepatocytes after bile duct ligation in chicken. Poult. Sci. Oct 8 2018 [PMID: 30295833] (IHC, Chicken) IHC Chicken
Show All 38 Publications.

Reviews for PCNA Antibody (NB500-106) (5) 4.85

Average Rating: 4.8
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Human, Mouse, Other.

Reviews using NB500-106:
Filter by Applications
WB
(2)
IHC-P
(1)
IF
(1)
IHC
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PCNA NB500-106
Enlarge
5
reviewed by:
Robert Lu
WB Human 12/17/2020
View

Summary

ApplicationWestern Blot
Sample TestedU-2 OS cells
SpeciesHuman
LotA-6

Comments

CommentsUsed at 1:2000 dilution in 0.5% milk PBST. Incubated overnight at 4C.
  5
reviewed by:
Verified Customer
WB Human 08/23/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:2000, overnight

Secondary Antibody

Secondary DescriptionSheep anti-mouse IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA931V
Secondary Concentration1:3000

Details

Detection NotesRegular Western blot Protocol
  5
reviewed by:
simon hardy
IHC-P Other 03/28/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedChicken intestine
SpeciesOther
CommentsVery strong signal at 1:1000. will try 1:2000 next time.

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionDAKO AEC kit

Details

Fixation Detailsformalin

Comments

CommentsVery strong signal at 1:1000. will try 1:2000 next time.
Immunofluorescence PCNA NB500-106
Enlarge
4
reviewed by:
Verified Customer
IF 02/24/2014
View

Summary

ApplicationImmunofluorescence
LotNB500-106SS Lot A
Immunohistochemistry PCNA NB500-106
Enlarge
5
reviewed by:
Verified Customer
IHC Mouse 02/06/2012
View

Summary

ApplicationImmunohistochemistry
Sample TestedMouse cornea
SpeciesMouse

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: 2 hours, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat, Secondary Ab Dilution Ratio: 1:500

Details

Fixation DetailsSample Preparation: Paraffin Section Method

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for PCNA Antibody (NB500-106). (Showing 1 - 3 of 3 FAQs).

  1. I am looking into preparing an exercise for an undergraduate lab that would immunostain drosophila testes. I think that PCNA Antibody (PC10) (cat # NB500-106) would be used as the primary antibody. What would you suggest to be used for the secondary? We have a fluorescent microscope and have used FITC in the past for some of our labs. Just trying to give the students a chance to visualize stained testes.
    • After looking at the applications of this antibody, I just found that we have not established this antibody for its Immunofluorescence application in our lab yet, however, as it has been tested successfully in IHC-P by our lab and by other researchers; there is a very strong chance that it should work in IHC-P/IF application also. If you would be interested in testing this novel application, please take a look at our Innovator's Reward Program. Regarding your specific query on secondary antibody, our FITC conjugated Mouse IgG antibody with catalog # NB720-F would be a perfect choice for primary PCNA Antibody (PC10) with catalog # NB500-106.
  2. Do you have data/information on the use of this antibody for Drosophila (mentioned on your website as possible target species)? I would like to know if any specific treatment is needed with regard to this type of sample and the use of the antibody in WB, IP in ICC.
    • This particular product is a widely licensed monoclonal clone. We obtained validation of its ability to detect the Drosophila protein from the following publication: PMID 22253867. You should be able to follow our general protocols for WB, IP, and ICC to detect this protein in their samples. Please see the following link for protocols.
  3. Can you please tell me the specific region of immunogen of this product?
    • This antibody was raised against whole rat PCNA protein fused to Protein A. As we have not yet epitope mapped this product, we do not know the exact immunogen recognized by this antibody.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for PCNA Antibody (NB500-106)

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Blogs on PCNA.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Caspase 3 - a Reliable Marker for Index of Apoptosis Induction
Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

PCNA Antibodies: Marking Cell Proliferation & DNA Replication
Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and ...  Read full blog post.

PCNA is a Universal Marker of Proliferating Cells
Proliferating cell nuclear antigen (PCNA) is an evolutionarily well-conserved protein found in all eukaryotic species as well as in Archaea. PCNA was first shown to be involved in DNA replication. However PCNA functions are associated with other vital...  Read full blog post.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and ...  Read full blog post.

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Recent Reviews

4.8
5
4
4
1
3
0
2
0
1
0

Robert Lu
12/17/2020
Application: WB
Species: Human

 
Verified Customer
08/23/2015
Application: WB
Species: Human

 
simon hardy
03/28/2014
Application: IHC-P
Species: Other

Bioinformatics

Gene Symbol PCNA
Entrez
Uniprot