PCNA Antibody (PC10)

Images

 
Western Blot: PCNA Antibody (PC10) [NB500-106] - Analysis of human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE lysate) cell extracts.
Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.
Western Blot: PCNA Antibody (PC10) [NB500-106] - Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a ...read more
Immunohistochemistry-Frozen: PCNA Antibody (PC10) [NB500-106] - IHC analysis of a paraformaldehyde fixed frozen section of brain from murine embryo using PCNA antibody clone PC10 (green), an IB4 antibody (red) and DAPI. ...read more
Immunohistochemistry-Paraffin: PCNA Antibody (PC10) [NB500-106] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization.
Simple Western: PCNA Antibody (PC10) [NB500-106] - Simple Western lane view shows a specific band for PCNA in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, ZeSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP, IHC-FrFl
Clone
PC10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

PCNA Antibody (PC10) Summary

Immunogen
Protein A-rat PCNA fusion obtained from pC2T
Marker
Proliferation Marker
Specificity
Specific for PCNA p36 protein expressed at high levels in proliferating cells.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PCNA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Simple Western 1:200
  • ELISA
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Immunoprecipitation 1:100
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry Free-Floating
Application Notes
This PCNA (PC10) antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation, Immunocytochemistry/Immunofluorescence (PMID: 22068968) Flow Cytometry and Western blot. It detects a band at 30 kDa by Western blot. For IHC on paraffin sections heat-mediated citrate buffer antigen retrieval is recommended.ELISA was reported in scientific literature. Use in chromatin immunoprecipitation reported in scientific literature (PMID 26883631). Use in Immunohistochemistry-free floating reported in scientific literature (PMID 27212918).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
PCNA Lysate (NBL1-14184)
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB500-106 in the following applications:

Publications
Read Publications using
NB500-106 in the following applications:

Reactivity Notes

Human, Yeast (S. pombe and S. cerevisiae), Drosophila melanogaster, Zebrafish, Rat, Mouse, Monkey, Chicken, Rabbit and Fish.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for PCNA Antibody (PC10)

  • cyclin
  • DNA polymerase delta auxiliary protein
  • MGC8367
  • proliferating cell nuclear antigen

Background

PCNA (Proliferating Cell Nuclear Antigen), plays a central role in coordinating the association of replication factors during DNA replication as well as during recognition and repair of DNA damage. PCNA forms a toroidal, ring-shaped structure of 90 kD by the symmetric association of three identical monomers. The ring encircles the DNA, acts as a platform upon which polymerases and other proteins dock to perform various DNA metabolic processes, and functions as a DNA polymerase-delta co-factor. The association of proteins with the replication fork through interactions with PCNA is regulated by several mechanisms. PCNA interacts with numerous proteins to facilitate DNA replication and repair, epigenetic processes and sister chromatid cohesion. PCNA has its highest expression during G1and S-phases, and its expression decreases in G2 and M-phases. This marker is also present in the early G0 phase because of its long half-live of 8-20 h. PCNA is a nuclear nonhistone protein that is necessary for DNA synthesis and is an accessory protein for DNA polymerase alpha, which is elevated during the G1/S phase of the cell cycle. Several studies have been performed to evaluate cell proliferation using PCNA and Ki-67 in different tumors of various origins; compared with PCNA, Ki-67 has been shown to be more sensitive and specific in the various tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PCNA Antibody (NB500-106)(21)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Fish, Porcine, Primate, Yeast.

We have publications tested in 9 applications: ChIP, ELISA, FLOW, ICC/IF, IHC, IHC-FrFl, IHC-P, IP, WB.


Filter By Application
ChIP
(1)
ELISA
(1)
FLOW
(1)
ICC/IF
(2)
IHC
(4)
IHC-FrFl
(1)
IHC-P
(9)
IP
(1)
WB
(6)
All Applications
Filter By Species
Human
(6)
Mouse
(5)
Rat
(4)
Fish
(2)
Porcine
(1)
Primate
(1)
Yeast
(1)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NB500-106 Applications Species
Xiong W, Luo Y, Wu L et al. Chemerin Stimulates Vascular Smooth Muscle Cell Proliferation and Carotid Neointimal Hyperplasia by Activating Mitogen-Activated Protein Kinase Signaling. PLoS ONE Oct 28 2016 [PMID: 27792753] (WB, Mouse) WB Mouse
Pushchina EV, Shukla S, Varaksin AA, Obukhov DK. Cell proliferation and apoptosis in optic nerve and brain integration centers of adult trout Oncorhynchus mykiss after optic nerve injury. Neural Regen Res. Apr 1 2016 [PMID: 27212918] (IHC-FrFl, Fish)

Details:
PCNA antibody used for IHC on optic nerves and brain of adult trout at a dilution of 1:3,000 at 4C for 2 days.
IHC-FrFl Fish
Hu Y, Cong X, Chen L et al. Synergy of TLR3 and 7 ligands significantly enhances function of DCs to present inactivated PRRSV antigen through TRIF/MyD88-NF-kB signaling pathway. Sci Rep. 2016 Apr 06 [PMID: 27046485] (WB, Porcine) WB Porcine
Ivanova IG, Maringele L. Polymerases epsilon and repair dysfunctional telomeres facilitated by salt. Nucleic Acids Res. 2016 Feb 15 [PMID: 26883631] (ChIP, Yeast) ChIP Yeast
Engler A, Tange C, Frank-Bertoncelj M et al. Regulation and function of SIRT1 in rheumatoid arthritis synovial fibroblasts. J. Mol. Med. 2015 Aug 23 [PMID: 26298564] (WB, Human) WB Human
Houlihan SL, Feng Y. The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation. Elife. 2014 Sep 23 [PMID: 25245017] (IHC-P, Mouse) IHC-P Mouse
Sawosz E, Jaworski S, Kutwin M et al. Toxicity of pristine graphene in experiments in a chicken embryo model Int J Nanomedicine 2014 Aug 25 [PMID: 25152621] (IHC) IHC
Zhu J, Zhu C, Ge S et al. Lactobacillus salivarius Ren prevent the early colorectal carcinogenesis in 1, 2-dimethylhydrazine-induced rat model. J. Appl. Microbiol. 2014 Apr 22 [PMID: 24754742] (IHC-P, Rat) IHC-P Rat
Zhang M, Wang F, Jiang L et al. Lactobacillus Salivarius REN Inhibits Rat Oral Cancer Induced by 4-Nitroquioline 1-Oxide. Cancer Prev Res (Phila) 2013 Jul [PMID: 23658366] (IHC-P, Rat) IHC-P Rat
Sheu MJ, Lin HY, Yang YH et al. Demethoxycurcumin, a major active curcuminoid from Curcuma longa, suppresses balloon injury induced vascular smooth muscle cell migration and neointima formation: An in vitro and in vivo study Mol Nutr Food Res 2013 Mar 21 [PMID: 23520190] (IHC-P, Rat) IHC-P Rat
Show All 21 Publications.

Reviews for PCNA Antibody (NB500-106) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Other.
We have 4 reviews tested in 4 application: WB, IHC-P, IF, IHC.

Reviews using NB500-106:
Filter by Applications
WB
(1)
IHC-P
(1)
IF
(1)
IHC
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 08/23/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:2000, overnight

Secondary Antibody

Secondary DescriptionSheep anti-mouse IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA931V
Secondary Concentration1:3000

Details

Detection NotesRegular Western blot Protocol
 
reviewed by:
simon hardy
IHC-P Other 03/28/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedChicken intestine
SpeciesOther
CommentsVery strong signal at 1:1000. will try 1:2000 next time.

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionDAKO AEC kit

Details

Fixation Detailsformalin

Comments

CommentsVery strong signal at 1:1000. will try 1:2000 next time.
Immunofluorescence PCNA NB500-106
Enlarge
reviewed by:
Anonymous
IF 02/24/2014
View

Summary

ApplicationImmunofluorescence
LotNB500-106SS Lot A
Immunohistochemistry PCNA NB500-106
Enlarge
reviewed by:
Anonymous
IHC Mouse 02/06/2012
View

Summary

ApplicationImmunohistochemistry
Sample TestedMouse cornea
SpeciesMouse

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: 2 hours, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat, Secondary Ab Dilution Ratio: 1:500

Details

Fixation DetailsSample Preparation: Paraffin Section Method

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PCNA Antibody (NB500-106). (Showing 1 - 3 of 3 FAQs).

  1. I am looking into preparing an exercise for an undergraduate lab that would immunostain drosophila testes. I think that PCNA Antibody (PC10) (cat # NB500-106) would be used as the primary antibody. What would you suggest to be used for the secondary? We have a fluorescent microscope and have used FITC in the past for some of our labs. Just trying to give the students a chance to visualize stained testes.
    • After looking at the applications of this antibody, I just found that we have not established this antibody for its Immunofluorescence application in our lab yet, however, as it has been tested successfully in IHC-P by our lab and by other researchers; there is a very strong chance that it should work in IHC-P/IF application also. If you would be interested in testing this novel application, please take a look at our Innovator's Reward Program. Regarding your specific query on secondary antibody, our FITC conjugated Mouse IgG antibody with catalog # NB720-F would be a perfect choice for primary PCNA Antibody (PC10) with catalog # NB500-106.
  2. Do you have data/information on the use of this antibody for Drosophila (mentioned on your website as possible target species)? I would like to know if any specific treatment is needed with regard to this type of sample and the use of the antibody in WB, IP in ICC.
    • This particular product is a widely licensed monoclonal clone. We obtained validation of its ability to detect the Drosophila protein from the following publication: PMID 22253867. You should be able to follow our general protocols for WB, IP, and ICC to detect this protein in their samples. Please see the following link for protocols.
  3. Can you please tell me the specific region of immunogen of this product?
    • This antibody was raised against whole rat PCNA protein fused to Protein A. As we have not yet epitope mapped this product, we do not know the exact immunogen recognized by this antibody.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-106AF405
Alexa Fluor 488 Labeled NB500-106AF488
Alexa Fluor 647 Labeled NB500-106AF647
Alexa Fluor 700 Labeled NB500-106AF700
Allophycocyanin Labeled NB500-106APC
DyLight 350 Labeled NB500-106UV
DyLight 405 Labeled NB500-106V
DyLight 488 Labeled NB500-106G
DyLight 550 Labeled NB500-106R
DyLight 650 Labeled NB500-106C
DyLight 680 Labeled NB500-106FR
DyLight 755 Labeled NB500-106IR
FITC Labeled NB500-106F
HRP Labeled NB500-106H
PE Labeled NB500-106PE
PerCP Labeled NB500-106PCP

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Blogs on PCNA.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Caspase 3 - a Reliable Marker for Index of Apoptosis Induction
Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

PCNA Antibodies: Marking Cell Proliferation & DNA Replication
Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and...  Read full blog post.

PCNA is a Universal Marker of Proliferating Cells
Proliferating cell nuclear antigen (PCNA) is an evolutionarily well-conserved protein found in all eukaryotic species as well as in Archaea. PCNA was first shown to be involved in DNA replication. However PCNA functions are associated with other vital...  Read full blog post.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and...  Read full blog post.

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

 
Anonymous
08/23/2015
Application: WB
Species: Human

 
simon hardy
03/28/2014
Application: IHC-P
Species: Other

Anonymous
02/24/2014
Application: IF
Species:

Bioinformatics

Gene Symbol PCNA
Entrez
Uniprot

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