PCNA Antibody (PC10)


Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - NIH3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at ...read more
Immunohistochemistry-Paraffin: PCNA Antibody (PC10) [NB500-106] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization.
Western Blot: PCNA Antibody (PC10) [NB500-106] - Analysis of human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE lysate) cell extracts.
Western Blot: PCNA Antibody (PC10) [NB500-106] - Western blot of PCNA levels across different stages of the cell cycle. U-2 OS cells were synchronised at G1/S with 2 mM thymidine an released for 10 hours for G2/M or ...read more
Immunohistochemistry-Frozen: PCNA Antibody (PC10) [NB500-106] - Paraformaldehyde fixed frozen section of brain from murine embryo using PCNA antibody clone PC10 (green), an IB4 antibody (red) and DAPI. Image provided by ...read more
Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - PC12 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at 1 ...read more
Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL ...read more
Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Development of the germinal zones in the tree shrew neocortex. Immunofluorescence for PCNA (yellow) and DAPI staining (blue) on 30 um-cryosections of E32-P1 tree ...read more
Flow Cytometry: PCNA Antibody (PC10) [NB500-106] - An intracellular stain was performed on HepG2 cells with PCNA Antibody (PC10) NB500-106G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more
Western Blot: PCNA Antibody (PC10) [NB500-106] - HeLa cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a ...read more
Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% ...read more
Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.

Product Details

Reactivity Hu, Mu, Rt, Po, Ch, Dr, Fi, Mar, Pm, Rb, Ye, ZeSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IP, ChIP, IHC
1 mg/ml

Order Details

PCNA Antibody (PC10) Summary

Protein A-rat PCNA fusion obtained from pC2T
Proliferation Marker
Specific for PCNA p36 protein expressed at high levels in proliferating cells.
IgG2a Kappa
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Chromatin Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature (PMID 26883631)
  • ELISA reported in scientific literature
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:2000. Use reported in scientific literature (PMID 22068968)
  • Immunohistochemistry Free-Floating reported in scientific literature (PMID 27212918)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Immunoprecipitation 1:100
  • Simple Western 1:200
  • Western Blot 1:2000
Application Notes
It detects a band at 30 kDa by Western blot. For IHC on paraffin sections, heat-mediated citrate buffer antigen retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
PCNA Overexpression Lysate
Reviewed Applications
Read 5 Reviews rated 4.8
NB500-106 in the following applications:

Read Publications using
NB500-106 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33802807). Use in Marsupial reported in scientific literature (PMID:29253253).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine, 0.15M NaCl
0.05% Sodium Azide
1 mg/ml
Protein A purified

Alternate Names for PCNA Antibody (PC10)

  • cyclin
  • DNA polymerase delta auxiliary protein
  • MGC8367
  • PCNA
  • proliferating cell nuclear antigen


PCNA (Proliferating Cell Nuclear Antigen), plays a central role in coordinating the association of replication factors during DNA replication as well as during recognition and repair of DNA damage. PCNA forms a toroidal, ring-shaped structure of 90 kD by the symmetric association of three identical monomers. The ring encircles the DNA, acts as a platform upon which polymerases and other proteins dock to perform various DNA metabolic processes, and functions as a DNA polymerase-delta co-factor. The association of proteins with the replication fork through interactions with PCNA is regulated by several mechanisms. PCNA interacts with numerous proteins to facilitate DNA replication and repair, epigenetic processes and sister chromatid cohesion. PCNA has its highest expression during G1and S-phases, and its expression decreases in G2 and M-phases. This marker is also present in the early G0 phase because of its long half-live of 8-20 h. PCNA is a nuclear nonhistone protein that is necessary for DNA synthesis and is an accessory protein for DNA polymerase alpha, which is elevated during the G1/S phase of the cell cycle. Several studies have been performed to evaluate cell proliferation using PCNA and Ki-67 in different tumors of various origins; compared with PCNA, Ki-67 has been shown to be more sensitive and specific in the various tumors.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
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Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: IHC, WB
Species: Hu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Species: Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ELISA
Species: Hu, Mu
Applications: IHC, WB
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
Species: Bv, Fe, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu, Pm
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IP, WB
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu
Applications: WB
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Mar, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IP, ChIP, IHC

Publications for PCNA Antibody (NB500-106)(42)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Chicken, Fish, Marsupial, Porcine, Primate, Yeast, Zebrafish.

We have publications tested in 10 applications: Chemotaxis, ELISA, FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-FrFl, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 42. Show All 42 Publications.
Publications using NB500-106 Applications Species
Rothermund K, Calabrese TC, Syed-Picard FN Differential Effects of Escherichia coli- Versus Porphyromonas gingivalis-derived Lipopolysaccharides on Dental Pulp Stem Cell Differentiation in Scaffold-free Engineered Tissues Journal of endodontics 2022-11-01 [PMID: 36108879]
Lundine D, Annor GK, Chavez V et al. The C-terminus of Gain-of-Function Mutant p53 R273H Is Required for Association with PARP1 and Poly-ADP-Ribose Molecular cancer research : MCR 2022-12-02 [PMID: 36074101] (WB, Human) WB Human
Meligy FY, El-Deen Mohammed HS, Mostafa TM et al. Therapeutic Potential of Mesenchymal Stem Cells versus Omega n - 3 Polyunsaturated Fatty Acids on Gentamicin-Induced Cardiac Degeneration Pharmaceutics 2022-06-22 [PMID: 35890218] (IHC-P, Rat)

Dilutions: 1:100
Martins RR, Zamzam M, Tracey-White D et al. MUller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina Aging cell 2022-03-22 [PMID: 35315590] (IF/IHC, Fish) IF/IHC Fish
Chung H, Moon S, Kang S Et al. Corneal Epithelial Removal with a Newly Designed Epithelial Brush J Ophthalmol 2022-02-14 [PMID: 35154818]
Bender H, Fietz Sa, Richter F, Stanojlovic M Alpha-Synuclein Pathology Coincides With Increased Number of Early Stage Neural Progenitors in the Adult Hippocampus Frontiers in cell and developmental biology 2021-07-07 [PMID: 34307368] (IF/IHC) IF/IHC
Sakurai R, Singh H, Wang Y et al. Effect of Perinatal Vitamin D Deficiency on Lung Mesenchymal Stem Cell Differentiation and Injury Repair Potential American journal of respiratory cell and molecular biology 2021-06-14 [PMID: 34126864]
Rossi G, Placidi M, Castellini C et al. Crocetin Mitigates Irradiation Injury in an In Vitro Model of the Pubertal Testis: Focus on Biological Effects and Molecular Mechanisms Molecules 2021-03-17 [PMID: 33802807] (WB, Mouse) WB Mouse
Verboom L, Martens A, Priem D et al. OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis Cell Rep 2020-02-18 [PMID: 32075762] (WB, Mouse) WB Mouse
Wong MK, Uchida M, Tsukada T Histological differentiation of mucus cell subtypes suggests functional compartmentation in the eel esophagus Cell Tissue Res. 2020-01-03 [PMID: 31900664]
Show All 42 Publications.

Reviews for PCNA Antibody (NB500-106) (5) 4.85

Average Rating: 4.8
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Human, Mouse, Other.

Reviews using NB500-106:
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Images Ratings Applications Species Date Details
Western Blot PCNA NB500-106
reviewed by:
Verified Customer
WB Human 12/17/2020


ApplicationWestern Blot
Sample TestedU-2 OS cells


CommentsUsed at 1:2000 dilution in 0.5% milk PBST. Incubated overnight at 4C.
reviewed by:
Haiquan Lu
WB Human 08/23/2015


ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
reviewed by:
Verified Customer
IHC-P Other 03/28/2014


Sample TestedChicken intestine
Immunofluorescence PCNA NB500-106
reviewed by:
Siegenthaler Lab
IF 02/24/2014


LotNB500-106SS Lot A
Immunohistochemistry PCNA NB500-106
reviewed by:
Bo-Yie Chen
IHC Mouse 02/06/2012


Sample TestedMouse cornea

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for PCNA Antibody (NB500-106). (Showing 1 - 3 of 3 FAQs).

  1. I am looking into preparing an exercise for an undergraduate lab that would immunostain drosophila testes. I think that PCNA Antibody (PC10) (cat # NB500-106) would be used as the primary antibody. What would you suggest to be used for the secondary? We have a fluorescent microscope and have used FITC in the past for some of our labs. Just trying to give the students a chance to visualize stained testes.
    • After looking at the applications of this antibody, I just found that we have not established this antibody for its Immunofluorescence application in our lab yet, however, as it has been tested successfully in IHC-P by our lab and by other researchers; there is a very strong chance that it should work in IHC-P/IF application also. If you would be interested in testing this novel application, please take a look at our Innovator's Reward Program. Regarding your specific query on secondary antibody, our FITC conjugated Mouse IgG antibody with catalog # NB720-F would be a perfect choice for primary PCNA Antibody (PC10) with catalog # NB500-106.
  2. Do you have data/information on the use of this antibody for Drosophila (mentioned on your website as possible target species)? I would like to know if any specific treatment is needed with regard to this type of sample and the use of the antibody in WB, IP in ICC.
    • This particular product is a widely licensed monoclonal clone. We obtained validation of its ability to detect the Drosophila protein from the following publication: PMID 22253867. You should be able to follow our general protocols for WB, IP, and ICC to detect this protein in their samples. Please see the following link for protocols.
  3. Can you please tell me the specific region of immunogen of this product?
    • This antibody was raised against whole rat PCNA protein fused to Protein A. As we have not yet epitope mapped this product, we do not know the exact immunogen recognized by this antibody.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on PCNA.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Caspase 3 - a Reliable Marker for Index of Apoptosis Induction
Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

PCNA Antibodies: Marking Cell Proliferation & DNA Replication
Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and ...  Read full blog post.

PCNA is a Universal Marker of Proliferating Cells
Proliferating cell nuclear antigen (PCNA) is an evolutionarily well-conserved protein found in all eukaryotic species as well as in Archaea. PCNA was first shown to be involved in DNA replication. However PCNA functions are associated with other vital...  Read full blog post.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and ...  Read full blog post.

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Recent Reviews


Verified Customer
Application: WB
Species: Human

Haiquan Lu
Application: WB
Species: Human

Verified Customer
Application: IHC-P
Species: Other


Gene Symbol PCNA