NG2/MCSP Antibody (LHM 2)

Images

 
Genetic Strategies: Knockout Validated: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NG2/MCSP (LHM2) knockout (KO) HeLa ...read more
Western Blot: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Analysis of NG2 expression in HeLa whole cell lysate.
Immunohistochemistry-Paraffin: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Staining of NG2 in human brain using DAB with hematoxylin counterstain.
Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on SK-MEL-28 cells with NG2/MCSP antibody (LHM 2) NB100-2688AF700 (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Antibody was tested at 1:400 in MCF7 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).
Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688PCP (blue) and a matched isotype control NBP2-27287PCP (orange). ...read more
Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688AF647 (blue) and a matched isotype control NBP2-27287AF647 ...read more
Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A surface stain was performed on A375 Cells with NG2/MCSP (LHM2) antibody NB100-2688 (blue) and a matched isotype control NBP1-43319 (orange). Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
Clone
LHM 2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

NG2/MCSP Antibody (LHM 2) Summary

Immunogen
A 375P cells crude extract
Localization
Cell Membrane
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CSPG4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:100
  • Flow (Cell Surface) 1 - 2.5 ug/mL
  • Knockout Validated
Application Notes
In WB a band can be seen at ~300 kDa.
Control
NG2/MCSP Knockout HeLa Cell Lysate
Publications
Read Publications using
NB100-2688 in the following applications:

  • 4 publications
  • IHC
    2 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for NG2/MCSP Antibody (LHM 2)

  • AN2
  • chondroitin sulfate proteoglycan 4 (melanoma-associated)
  • chondroitin sulfate proteoglycan 4
  • CSPG4
  • EC 3.6.3
  • HMW-MAA
  • MCSP
  • MCSPChondroitin sulfate proteoglycan NG2
  • MCSPG
  • MCSPGMelanoma chondroitin sulfate proteoglycan
  • MEL-CSPG
  • MSK16Melanoma-associated chondroitin sulfate proteoglycan
  • NG2
  • NG2EC 2.7.8

Background

NG2 (Neuron Glial Antigen 2), also known as MCSP, and CSPG4 is a chondroitin surface proteoglycan. Chondroitin surface proteoglycans play a role in stabilizing cell-substratum interactions during early events of cell spreading. At large, proteoglycans are important for cell proliferation and migration, and may act as signal transducers through C-terminal cytoplasmic binding to scaffolding or signaling proteins. The NG2 glycoprotein is a type I membrane protein expressed in the developing and adult central nervous system (CNS) by subpopulations of glia including oligodendroglial precursor cells (OPCs), and in the developing CNS additionally by pericytes. NG2 is located at the apical cell membrane, however it has also been shown to localize to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Research has found that NG2 may inhibit neuronal growth (PMC1571583), and also acts as a proapoptotic receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NG2/MCSP Antibody (NB100-2688)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(4)
IHC
(2)
WB
(1)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-2688 Applications Species
Li Xu, Guo Weimin, Zha Kangkang et al. Enrichment of CD146+ Adipose-Derived Stem Cells in Combination with Articular Cartilage Extracellular Matrix Scaffold Promotes Cartilage Regeneration. Theranostics 2019 [PMID: 31410204] (WB, Human) WB Human
Arrigoni C, Bongio M, Talo G et al. Rational Design of Prevascularized Large 3D Tissue Constructs Using Computational Simulations and Biofabrication of Geometrically Controlled Microvessels. Adv Healthc Mater. May 18 2016 [PMID: 27191352] (ICC/IF, Human) ICC/IF Human
Kinney HC, Haynes RL, Xu G, Andiman SE, Folkerth RD, Sleeper LA, Volpe JJ. Neuron deficit in the white matter and subplate in periventricular leukomalacia. Ann Neurol;71(3):397-406. 2012 Mar. [PMID: 22451205] (ICC/IF, IHC, Human) ICC/IF, IHC Human
Pierce GL, Donato AJ, LaRocca TJ, Eskurza I, Silver AE, Seals DR. Habitually exercising older men do not demonstrate age-associated vascular endothelial oxidative stress. Aging Cell;10(6):1032-7. 2011 Dec. [PMID: 21943306] (ICC/IF, Human) ICC/IF Human
Wang Y-C, Nakagawa M, Garitaonandia I et al. Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis. Cell Research. 2011 Sep 6. [PMID: 21894191]
Kose O, Kutulola AO, Odell EW, Waseem A. Changes in the expression of stem cell markers in oral lichen planus and hyperkeratotic lesions. J Oral Sci. 2007 June [PMID: 17634726] (IHC, Human) IHC Human
Legg J, Jensen UB, Broad S et al. Role of melanoma chondroitin sulphate proteoglycan in patterning stem cells in human interfollicular epidermis. Development. 2003 Dec [PMID: 14573520] (ICC/IF, Human) ICC/IF Human

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NG2/MCSP Antibody (NB100-2688) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2688AF350
Alexa Fluor 405 NB100-2688AF405
Alexa Fluor 488 NB100-2688AF488
Alexa Fluor 532 NB100-2688AF532
Alexa Fluor 594 NB100-2688AF594
Alexa Fluor 647 NB100-2688AF647
Alexa Fluor 700 NB100-2688AF700
Alexa Fluor 750 NB100-2688AF750
Allophycocyanin NB100-2688APC
Allophycocyanin/Cy7 NB100-2688APCCY7
Biotin NB100-2688B
DyLight 350 NB100-2688UV
DyLight 405 NB100-2688V
DyLight 488 NB100-2688G
DyLight 550 NB100-2688R
DyLight 594 NB100-2688DL594
DyLight 650 NB100-2688C
DyLight 680 NB100-2688FR
DyLight 755 NB100-2688IR
FITC NB100-2688F
HRP NB100-2688H
Janelia Fluor 549 NB100-2688JF549
Janelia Fluor 646 NB100-2688JF646
PE NB100-2688PE
PE/Atto594 NB100-2688PEATT594
PE/Cy5.5 NB100-2688PECY55
PE/Cy7 NB100-2688PECY7
PerCP NB100-2688PCP

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Bioinformatics

Gene Symbol CSPG4
Entrez
Uniprot