NG2/MCSP Antibody (LHM 2)

Images

 
Western Blot: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Analysis of NG2 expression in HeLa whole cell lysate.
Immunohistochemistry-Paraffin: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Staining of NG2 in human brain using DAB with hematoxylin counterstain.
Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A surface stain was performed on A375 Cells with NG2/MCSP (LHM2) antibody NB100-2688 (blue) and a matched isotype control NBP1-43319 (orange). Cells were ...read more
Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Antibody was tested at 1:400 in MCF7 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).
Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688PCP (blue) and a matched isotype control NBP2-27287PCP (orange). ...read more
Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688AF647 (blue) and a matched isotype control NBP2-27287AF647 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
LHM 2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NG2/MCSP Antibody (LHM 2) Summary

Immunogen
A 375P cells crude extract
Localization
Cell Membrane
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CSPG4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:100
  • Flow (Cell Surface) 1-2.5 ug/mL
Application Notes
This NG2 Antibody (LHM 2) is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on both frozen and paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, a band can be seen at ~300 kDa.
Publications
Read Publications using
NB100-2688 in the following applications:

Reactivity Notes

Human. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for NG2/MCSP Antibody (LHM 2)

  • AN2
  • chondroitin sulfate proteoglycan 4 (melanoma-associated)
  • chondroitin sulfate proteoglycan 4
  • CSPG4
  • EC 3.6.3
  • HMW-MAA
  • MCSP
  • MCSPChondroitin sulfate proteoglycan NG2
  • MCSPG
  • MCSPGMelanoma chondroitin sulfate proteoglycan
  • MEL-CSPG
  • MSK16Melanoma-associated chondroitin sulfate proteoglycan
  • NG2
  • NG2EC 2.7.8

Background

NG2 (Neuron Glial Antigen 2), also known as MCSP, and CSPG4 is a chondroitin surface proteoglycan. Chondroitin surface proteoglycans play a role in stabilizing cell-substratum interactions during early events of cell spreading. At large, proteoglycans are important for cell proliferation and migration, and may act as signal transducers through C-terminal cytoplasmic binding to scaffolding or signaling proteins. The NG2 glycoprotein is a type I membrane protein expressed in the developing and adult central nervous system (CNS) by subpopulations of glia including oligodendroglial precursor cells (OPCs), and in the developing CNS additionally by pericytes. NG2 is located at the apical cell membrane, however it has also been shown to localize to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Research has found that NG2 may inhibit neuronal growth (PMC1571583), and also acts as a proapoptotic receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NG2/MCSP Antibody (NB100-2688)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IHC.


Filter By Application
ICC/IF
(4)
IHC
(2)
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Filter By Species
Human
(5)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NG2/MCSP Antibody (NB100-2688) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2688AF405
Alexa Fluor 488 Labeled NB100-2688AF488
Alexa Fluor 647 Labeled NB100-2688AF647
Alexa Fluor 700 Labeled NB100-2688AF700
Allophycocyanin Labeled NB100-2688APC
DyLight 350 Labeled NB100-2688UV
DyLight 405 Labeled NB100-2688V
DyLight 488 Labeled NB100-2688G
DyLight 680 Labeled NB100-2688FR
DyLight 755 Labeled NB100-2688IR
FITC Labeled NB100-2688F
PE Labeled NB100-2688PE
PerCP Labeled NB100-2688PCP

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Bioinformatics

Gene Symbol CSPG4
Entrez
Uniprot