NG2/MCSP Antibody (LHM 2)


Genetic Strategies: Knockout Validated: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NG2/MCSP (LHM2) knockout (KO) HeLa more
Western Blot: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Analysis of NG2 expression in HeLa whole cell lysate.
Immunohistochemistry-Paraffin: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Staining of NG2 in human brain using DAB with hematoxylin counterstain.
Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on SK-MEL-28 cells with NG2/MCSP antibody (LHM 2) NB100-2688AF700 (blue) and a matched isotype control (orange). Cells were more
Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Antibody was tested at 1:400 in MCF7 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).
Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688PCP (blue) and a matched isotype control NBP2-27287PCP (orange). more
Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688AF647 (blue) and a matched isotype control NBP2-27287AF647 more
Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A surface stain was performed on A375 Cells with NG2/MCSP (LHM2) antibody NB100-2688 (blue) and a matched isotype control NBP1-43319 (orange). Cells were more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, KO
1.0 mg/ml
Validated by:

Genetic Strategies


Order Details

NG2/MCSP Antibody (LHM 2) Summary

A 375P cells crude extract
Cell Membrane
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow (Cell Surface) 1 - 2.5 ug/mL
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:100
  • Knockout Validated
  • Western Blot 1:1000
Application Notes
In WB a band can be seen at ~300 kDa.
Read Publications using
NB100-2688 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine, 0.15 M NaCl
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for NG2/MCSP Antibody (LHM 2)

  • AN2
  • chondroitin sulfate proteoglycan 4 (melanoma-associated)
  • chondroitin sulfate proteoglycan 4
  • CSPG4
  • EC 3.6.3
  • MCSP
  • MCSPChondroitin sulfate proteoglycan NG2
  • MCSPGMelanoma chondroitin sulfate proteoglycan
  • MSK16Melanoma-associated chondroitin sulfate proteoglycan
  • NG2
  • NG2EC 2.7.8


NG2 (Neuron Glial Antigen 2), also known as MCSP, and CSPG4 is a chondroitin surface proteoglycan. Chondroitin surface proteoglycans play a role in stabilizing cell-substratum interactions during early events of cell spreading. At large, proteoglycans are important for cell proliferation and migration, and may act as signal transducers through C-terminal cytoplasmic binding to scaffolding or signaling proteins. The NG2 glycoprotein is a type I membrane protein expressed in the developing and adult central nervous system (CNS) by subpopulations of glia including oligodendroglial precursor cells (OPCs), and in the developing CNS additionally by pericytes. NG2 is located at the apical cell membrane, however it has also been shown to localize to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Research has found that NG2 may inhibit neuronal growth (PMC1571583), and also acts as a proapoptotic receptor.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NG2/MCSP Antibody (NB100-2688)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 10.
Publications using NB100-2688 Applications Species
Koivunotko E, Snirvi J, Merivaara A Et al. Angiogenic Potential of Human Adipose-Derived Mesenchymal Stromal Cells in Nanofibrillated Cellulose Hydrogel Biomedicines 2022-10-27 [PMID: 36289846] (WB, Human)

Citation using the Azide and BSA Free version of this antibody.
WB Human
Lin Z, Zhang X, Fritch MR Et al. Engineering pre-vascularized bone-like tissue from human mesenchymal stem cells through simulating endochondral ossification Biomaterials 2022-03-08 [PMID: 35259584] (IHC-P, Human)

Citation using the Alexa Fluor 594 version of this antibody.
IHC-P Human
Liu HY, Koch C, Haller A Et al. Evaluation of Microfluidic Ceiling Designs for the Capture of Circulating Tumor Cells on a Microarray Platform Adv Biosyst 2020-04-16 [PMID: 32293134]

Citation using the PE version of this antibody.
Li X, Guo W, Zha K et al. Enrichment of CD146+ Adipose-Derived Stem Cells in Combination with Articular Cartilage Extracellular Matrix Scaffold Promotes Cartilage Regeneration Theranostics 2019-07-09 [PMID: 31410204] (WB, Human) WB Human
Arrigoni C, Bongio M, Talo G et al. Rational Design of Prevascularized Large 3D Tissue Constructs Using Computational Simulations and Biofabrication of Geometrically Controlled Microvessels. Adv Healthc Mater. 2016-05-18 [PMID: 27191352] (ICC/IF, Human) ICC/IF Human
Kinney HC, Haynes RL, Xu G, Andiman SE, Folkerth RD, Sleeper LA, Volpe JJ. Neuron deficit in the white matter and subplate in periventricular leukomalacia. Ann Neurol;71(3):397-406. 2012-03-01 [PMID: 22451205] (IF/IHC, ICC/IF, Human) IF/IHC, ICC/IF Human
Pierce GL, Donato AJ, LaRocca TJ, Eskurza I, Silver AE, Seals DR. Habitually exercising older men do not demonstrate age-associated vascular endothelial oxidative stress. Aging Cell;10(6):1032-7. 2011-12-01 [PMID: 21943306] (ICC/IF, Human) ICC/IF Human
Wang Y-C, Nakagawa M, Garitaonandia I et al. Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis. Cell Research. 2011-09-06 [PMID: 21894191]
Kose O, Kutulola AO, Odell EW, Waseem A. Changes in the expression of stem cell markers in oral lichen planus and hyperkeratotic lesions. J Oral Sci. 2007-06-01 [PMID: 17634726] (IF/IHC, Human) IF/IHC Human
Legg J, Jensen UB, Broad S et al. Role of melanoma chondroitin sulphate proteoglycan in patterning stem cells in human interfollicular epidermis. Development. 2003-12-01 [PMID: 14573520] (ICC/IF, Human) ICC/IF Human

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


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Gene Symbol CSPG4