Western Blot: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Analysis of NG2 expression in HeLa whole cell lysate.
Immunohistochemistry-Paraffin: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Staining of NG2 in human brain using DAB with hematoxylin counterstain.
Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A surface stain was performed on A375 Cells with NG2/MCSP (LHM2) antibody NB100-2688 (blue) and a matched isotype control NBP1-43319 (orange). Cells were ...read more
Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Antibody was tested at 1:400 in MCF7 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).
Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688PCP (blue) and a matched isotype control NBP2-27287PCP (orange). ...read more
Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688AF647 (blue) and a matched isotype control NBP2-27287AF647 ...read more
This NG2 Antibody (LHM 2) is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on both frozen and paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, a band can be seen at ~300 kDa.
Read Publications using NB100-2688 in the following applications:
NG2 (Neuron Glial Antigen 2), also known as MCSP, and CSPG4 is a chondroitin surface proteoglycan. Chondroitin surface proteoglycans play a role in stabilizing cell-substratum interactions during early events of cell spreading. At large, proteoglycans are important for cell proliferation and migration, and may act as signal transducers through C-terminal cytoplasmic binding to scaffolding or signaling proteins. The NG2 glycoprotein is a type I membrane protein expressed in the developing and adult central nervous system (CNS) by subpopulations of glia including oligodendroglial precursor cells (OPCs), and in the developing CNS additionally by pericytes. NG2 is located at the apical cell membrane, however it has also been shown to localize to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Research has found that NG2 may inhibit neuronal growth (PMC1571583), and also acts as a proapoptotic receptor.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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