| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-15160SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-119SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60531SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77686SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27103SS | |||
| Azide and BSA Free | NBP2-80749 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-16518SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-556-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-40766SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-231SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-41123SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-266SS | |||
| Azide and BSA Free | NBP2-80869 | |||
![Immunohistochemistry-Paraffin: Chromogranin A Antibody [NB120-15160] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse pancreas using Chromogranin A antibody at a dilution of 1:500. The signal was developed using HRP-conjugated anti-rabbit secondary and DAB reagent which followed counterstaining of the section with hematoxylin. The antibody generated a strong staining in Islets of Langerhans. Also, in the periphery of the cells, the pattern of staining was punctate representing the vesicles with Chromogranin A. Cells of lobules and acini showed a weak cytoplasmic and nuclear staining which is potentially the secreted pool of Chromogranin A.](http://images.novusbio.com/fullsize/Chromogranin-A-Antibody-Immunohistochemistry-Paraffin-NB120-15160-img0003.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody [NB120-15160] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse pancreas using Chromogranin A antibody at a dilution of 1:500. The signal was developed using HRP-conjugated anti-rabbit secondary and DAB reagent which followed counterstaining of the section with hematoxylin. The antibody generated a strong staining in Islets of Langerhans. Also, in the periphery of the cells, the pattern of staining was punctate representing the vesicles with Chromogranin A. Cells of lobules and acini showed a weak cytoplasmic and nuclear staining which is potentially the secreted pool of Chromogranin A.")
![Immunohistochemistry: Chromogranin A Antibody [NB120-15160] - IHC-P analysis fo human pancreas tissue showing specific staining of Chromogranin A in the Islets of Langerhans (dark brown staining).](http://images.novusbio.com/fullsize/Chromogranin-A-Antibody-Immunohistochemistry-NB120-15160-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - The frequency of chromogranin A positive hormone-negative (CPHN) cells decreases with age. Representative pancreatic sections from fetal (A) and infant-child (B-D) cases immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, and ghrelin) (white), chromogranin A (green), and DAPI (blue). Yellow arrows show CPHN cells. Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fendo.2018.00791/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Chromogranin-A-Antibody-Immunocytochemistry-Immunofluorescence-NB120-15160-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - SH-SY5Y cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Chromogranin A at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Chromogranin-A-Antibody-Immunocytochemistry-Immunofluorescence-NB120-15160-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - Mouse pancreas cryosections stained with insulin (DAKO, Green) and Chromogranin A (NB120-15160; 1:500; White)](http://images.novusbio.com/fullsize/Chromogranin-A-Antibody-Immunocytochemistry-Immunofluorescence-NB120-15160-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Chromogranin A Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Chromogranin-A-Antibody-Immunocytochemistry-Immunofluorescence-NB120-15160-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] - ChromograninA positive hormone-negative (CPHN) cells do not replicate during fetal & infant life. Representative pancreatic sections from fetal (A) & infant (B) donors immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, & ghrelin) (white), chromograninA (green), Ki67 (red), & DAPI (blue). Yellow arrows indicate CPHN cells. CPHN cells were rarely positive for Ki67 staining in both fetal & infant groups; no detectable difference was found in the frequency of replicative CPHN cells between fetal & infant pancreatic sections. Scale bars: 100 μm for low power & 50 μm for high magnification images. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30687234), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-15160_rabbit-polyclonal-chromogranin-a-antibody-31020241539710.jpg)
![Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] - ChromograninA positive hormone-negative (CPHN) cells express the beta-cell differentiation transcription factor NKX2.2 in both fetuses & infants. Representative pancreatic sections from fetal (A) & infant (B) donors immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, & ghrelin) (white), chromograninA (green), the transcription factor NKX2.2 (red) & DAPI (blue). Yellow arrows indicate CPHN cells. Scale bars: 100 μm for low power & 25 μm for high magnification images. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30687234), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-15160_rabbit-polyclonal-chromogranin-a-antibody-31020241539729.jpg)
![Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] - ChromograninA positive hormone-negative (CPHN) cells express the endocrine differentiation transcription factor NKX6.1 in both fetal & infant pancreas. Representative pancreatic sections from fetal (A) & infant (B) donors immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, & ghrelin) (white), chromograninA (green), the transcription factor NKX6.1 (red) & DAPI (blue). Yellow arrows indicate CPHN cells. Scale bars: 100 μm for low power & 25 μm for high magnification images. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30687234), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-15160_rabbit-polyclonal-chromogranin-a-antibody-310202415175268.jpg)
![Western Blot: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Analysis of Neurokinin 1 Receptor in 1: human brain lysate, 2: rat brain lysate and 3: monkey brain lysate.](http://images.novusbio.com/fullsize/NK1R-Antibody-Western-Blot-NB300-119-img0009.jpg "Western Blot: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Analysis of Neurokinin 1 Receptor in 1: human brain lysate, 2: rat brain lysate and 3: monkey brain lysate.")
![Immunocytochemistry/Immunofluorescence: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Immunostain of NK1R in rat enteric glial cells. Image from verified customer review.](http://images.novusbio.com/fullsize/NK1R-Antibody-Immunocytochemistry-Immunofluorescence-NB300-119-img0004.jpg)
![Immunohistochemistry: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Staining of human brain, basal nucleus of Meynert, neurons.](http://images.novusbio.com/fullsize/NK1R-Antibody-Immunohistochemistry-NB300-119-img0006.jpg)
![Western Blot: NK1R Antibody [NB300-119] - Total protein from human, mouse and rat brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-NK1R in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/NK1R-Antibody-Western-Blot-NB300-119-img0010.jpg)
![Western Blot: NK1R Antibody [NB300-119] - TACR1 and its downstream targets SRC and p-SRC are expressed in neuroblastoma cell lines. Endogenous TACR1 (NK1R), SRC and p-SRC expression in whole-cell extracts of neuroblastoma cell lines was visualized by western blotting. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.13440), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NK1R-Antibody-Western-Blot-NB300-119-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: NK1R Antibody [NB300-119] - Overnight incubation at 4C with NK1R antibody diluted 1:100 in PBS/BSA 1%. Red: Phalloidin, Blue: DAPI, Green: NK1R antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-119_rabbit-polyclonal-nk1r-antibody-immunocytochemistry-immunofluorescence-1372023145313..jpg)
![Western Blot: NK1R Antibody - BSA Free [NB300-119] - TACR1 & its downstream targets SRC & p-SRC are expressed in neuroblastoma cell lines(A) Endogenous TACR1, SRC & p-SRC expression in whole-cell extracts of neuroblastoma cell lines was visualized by western blotting. GAPDH was used as a loading control. (B) Quantitative RT-PCR of TACR1 mRNA in a panel of neuroblastoma cell lines (n = 3, error bars indicate standard deviation). (C–E) Quantification of SRC (C), p-SRC (D) & TACR1 (E) protein expression using densitometry analysis of western immunoblots (n = 3, error bars indicate standard deviation). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.13440), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-119_rabbit-polyclonal-nk1r-antibody-310202415392524.jpg)
![Western Blot: NK1R Antibody - BSA Free [NB300-119] - Striatal beta 3 subunit protein reduced conditional KO mice. (A) Fluorescent images demonstrating colocalization between endogenous tdTomato fluorescence & DARPP-32 expression. Approximately half of the DARPP-32+ cells expressed endogenous tdTomato, indicative of Cre expression. Scale bar is 25 μm. (B) Representative western blot analysis of the GABAA receptor beta 3 subunit from individual 30-day-old mice revealed reduced amounts of beta 3 subunit protein in striatum (B) from Cre positive ( beta 3f/fDrd2) animals compared to Cre negative ( beta 3f/+ & beta 3f/f) control animals. The amount of beta 3 protein in cortex (C) did not differ between genotypes. Blots were reprobed for beta -actin. (D) Summary graph of western blot analysis demonstrating a significant reduction in beta 3 protein in striatum, but not cortex. Data are expressed as percent change in band intensity relative to Cre negative controls following normalization to actin. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21847370), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-119_rabbit-polyclonal-nk1r-antibody-310202416205147.jpg)
![Western Blot: NK1R Antibody - BSA Free [NB300-119] - Striatal beta 3 subunit protein reduced conditional KO mice. (A) Fluorescent images demonstrating colocalization between endogenous tdTomato fluorescence & DARPP-32 expression. Approximately half of the DARPP-32+ cells expressed endogenous tdTomato, indicative of Cre expression. Scale bar is 25 μm. (B) Representative western blot analysis of the GABAA receptor beta 3 subunit from individual 30-day-old mice revealed reduced amounts of beta 3 subunit protein in striatum (B) from Cre positive ( beta 3f/fDrd2) animals compared to Cre negative ( beta 3f/+ & beta 3f/f) control animals. The amount of beta 3 protein in cortex (C) did not differ between genotypes. Blots were reprobed for beta -actin. (D) Summary graph of western blot analysis demonstrating a significant reduction in beta 3 protein in striatum, but not cortex. Data are expressed as percent change in band intensity relative to Cre negative controls following normalization to actin. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21847370), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-119_rabbit-polyclonal-nk1r-antibody-310202416212385.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Immunohistochemistry-Paraffin-NB110-60531-img0011.jpg "Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.")
![Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Western-Blot-NB110-60531-img0013.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0014.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - 50 ug protein per lane for liver lysate and 0.5 ul of plasma at 9% SDS. Samples are loaded onto the gel as the following order: human liver lysate, mouse liver lysate, rat liver lysate, human plasma, and mouse plasma. Image from confirmed customer review.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Western-Blot-NB110-60531-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ApoE (WUE-4) [NB110-60531] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Immunocytochemistry-Immunofluorescence-NB110-60531-img0010.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - Analysis of apoB-positivity in blood plasma and PLT concentrate-derived EXOs. FCM detection of the indicated markers in EXOs conjugated onto latex beads. The EXOs were isolated from fasting PFP or PLT concentrate by differential UC and gravity size filtration (gray histograms: blocked beads incubated with antibody, empty histograms: EXO sample). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep24316), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0020.jpg)
![Flow (Intracellular): Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with NB110-60531AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Intracellular-NB110-60531-img0012.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with NB110-60531PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0015.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with Apolipoprotein E/ApoE Antibody NB110-60531 [WUE-4] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0016.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with alpha-Synuclein [2A7] Antibody NB110-60531AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0017.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with Apolipoprotein E/ApoE Antibody [WUE-4] NB110-60531F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0018.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with Apolipoprotein E/ApoE [WUE-4] Antibody NB110-60531PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0019.jpg)
![Simple Western: GAPDH Antibody [NB300-322] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-322-img0005.jpg "Simple Western: GAPDH Antibody [NB300-322] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.")
![Western Blot: GAPDH Antibody [NB300-322] - Detection of Human and Mouse GAPDH (theoretical molecular weight: 36 kDa) by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-322 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0007.jpg)
![Western Blot: GAPDH Antibody [NB300-322] - Detection of Human and Mouse GAPDH (theoretical molecular weight: 36 kDa) by Western Blot. Samples: Whole cell lysate from mouse NIH3T3 (5, 15 and 50 ug) and human HeLa (H; 50 ug) cells. Antibody: Affinity purified rabbit anti-GAPDH antibody used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0006.jpg)
![Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-322-img0008.jpg)
![Western Blot: GAPDH Antibody [NB300-322] - c-Src is required for efficient translation of PRC2 component mRNAs. Immunoblot of PRC2 components in control and c-Src-deficient tumors. Bar chart shows quantification of fluorescent immunoblot data normalized to the loading control (Gapdh). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10681-4), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-322] - GAPDH detection in HeLa cells with ICC-IF application using NB300-322, visualized with DyLight Fluor 488.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NB300-322-img0003.jpg)
![Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-322-img0009.jpg)
![Western Blot: GAPDH Antibody [NB300-322] - Mouse DRG stained at 1:2000 dilution. Image provided by verified customer review.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0004.jpg)
![Western Blot: GAPDH Antibody [NB300-322] - Gem expression is sufficient to increase chemokinesis and chemotaxis. Western blot analyses were performed on 70 ug of cellular extracts from MOLT4 cells transduced by Lenti-control or Lenti-GEM viral particles. Membranes were probed with anti-Gem (1:2,000) or anti-GAPDH (1:1,000) antibody. ***: significantly different, p 0.1), Fgenotype(1,30) = 30.7 (p Int J Mol Sci (2024). Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-12220251513483.jpg)
![Simple Western: GAPDH Antibody [NB300-322] - Astroglial expression in the plasma membrane evoked by ripple burst and fast ripple burst stimulations in wild type and S286L-TG. Panel (A) indicates expression of pannexin1 in the astroglial plasma membrane fraction. Ordinates indicate the mean ± SD (n = 6) of relative expression of pannexin1 per GAPDH. Gray and green bars indicate pannexin1 expression in astrocytes of wild type and S286L-TG after chronic fast ripple-evoked stimulation, respectively. * p 0.1), Fgenotype*age(1,20) = 5.4 (p Int J Mol Sci (2024). Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-12220251513482.jpg)
![Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-77686] - IHC analysis of formalin fixed paraffin embedded tissue section from hippocampus of mouse brain with NeuN antibody at 1:200 dilution. The antibody generated a strong nuclear with weak cytoplasmic staining in NeuN reactive cells.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Immunohistochemistry-Paraffin-NBP1-77686-img0007.jpg "Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-77686] - IHC analysis of formalin fixed paraffin embedded tissue section from hippocampus of mouse brain with NeuN antibody at 1:200 dilution. The antibody generated a strong nuclear with weak cytoplasmic staining in NeuN reactive cells.")
![Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-77686] - JAK3 colocalized with neurons labeled with NeuN. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fneur.2017.00363/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Immunohistochemistry-Paraffin-NBP1-77686-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody [NBP1-77686] - Neuro2a cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-RBFOX3/NeuN Antibody NBP1-77686 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77686-img0013.jpg)
![Western Blot: RBFOX3/NeuN Antibody [NBP1-77686] - Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Western-Blot-NBP1-77686-img0012.jpg)
![Flow Cytometry: RBFOX3/NeuN Antibody [NBP1-77686] - An intracellular stain was performed on Hek293 cells with NBP1-77686AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Flow-Cytometry-NBP1-77686-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody [NBP1-77686] - NeuN antibody was tested in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77686-img0005.jpg)
![Immunohistochemistry: RBFOX3/NeuN Antibody [NBP1-77686] - Analysis of NeuN in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Immunohistochemistry-NBP1-77686-img0008.jpg)
![Flow Cytometry: RBFOX3/NeuN Antibody [NBP1-77686] - An intracellular stain was performed on U2OS cells with RBFOX3/NeuN Antibody NBP1-77686AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/RBFOX3-NeuN-Antibody-Flow-Cytometry-NBP1-77686-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody - BSA Free [NBP1-77686] - Double immunofluorescence indicates that NF-kappa B & CCL5 were co-localized with microglia, astrocytes, & neurons in the ipsilateral L4–5 spinal cord on day 7 after CCI.NF-kappa B (green) colocalizes with Iba-1, GFAP, & NeuN (red) in laminae II–III of the superficial dorsal horn (A). CCL5 (red) co-localizes with Iba-1, GFAP, & NeuN (green) in the medial superficial dorsal horn (laminae II–III) (B). NF-kappa B (green) co-localizes with CCL5 (red) in laminae II–IV of the superficial dorsal horn following CCI on day 7 in the sham & CCI groups in the ipsilateral L4–5 spinal cord (C). Two single stained images (yellow) were merged. Scale = 100 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0115120), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77686_rabbit-polyclonal-rbfox3-neun-antibody-31020241538241.jpg)
![Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody - BSA Free [NBP1-77686] - (A) Representative Western blots depicting phosphorylated JAK3 (pJAK3), total JAK3, & corresponding beta -actin levels in the ipsilateral (CXI) versus the contralateral (CXC) cortex at 24 h in sham & mice subjected to permanent middle cerebral artery occlusion (pMCAO). (B,C) Quantified pJAK3 & JAK3 values normalized to beta -actin in the cortex of sham & mice subjected to stroke. Both pJAK3 & JAK3 are significantly increased in the ipsilateral cortex of stroked animals (*P < 0.05 & **P < 0.01 with respect to sham ipsilateral/contralateral & stroke contralateral; sham, n = 5; stroke, n = 13). Data were analyzed using a two-way ANOVA (ipsilateral/contralateral or sham/stroke) with Bonferroni post-test. (D) Diagram showing peri-infarct brain region from which images were taken (n = 3). Scale bar is 10 µm, & magnification is 60×. (E) JAK3 colocalized with microglia/macrophages labeled with Iba-1. (F) JAK3 colocalized with neurons labeled with NeuN. (G) JAK3 colocalized with endothelial cells labeled with CD31. (H) JAK3 is not colocalized with astrocytes labeled with GFAP. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28790974), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77686_rabbit-polyclonal-rbfox3-neun-antibody-310202416175267.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (13H12) [NBP2-27103] - GAPDH antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Immunocytochemistry-Immunofluorescence-NBP2-27103-img0013.jpg "Immunocytochemistry/Immunofluorescence: GAPDH Antibody (13H12) [NBP2-27103] - GAPDH antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A dilution of 1:10 was used. Image objective 40x.")
![Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - WB detection of GAPDH protein (theoretical molecular weight: 36 kDa) in HeLa cells lysate using GAPDH antibody (clone 13H12) in (A) the absence and (B) the presence of immunizing peptide.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Western-Blot-NBP2-27103-img0001.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of human rectal carcinoma tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Immunohistochemistry-Paraffin-NBP2-27103-img0011.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of normal human breast tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Immunohistochemistry-Paraffin-NBP2-27103-img0008.jpg)
![Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - Characterization of lncR492.(B) Northern blot of lncR494 using increasing amounts of loaded total. Black arrow signifies lncR492-specific signal at approximately 1400 bp. A probe targeting Gapdh mRNA served as the loading control. (D) RT-PCR analysis of lncR492 and Gapdh expression. RNA extract was treated with a 5'-phosphate-dependent exonuclease, resulting in a degradation of f.ex. ribosomal RNA.Citation: Winzi M, Casas Vila N, Paszkowski-Rogacz M, Ding L, Noack S, Theis M, et al. (2018) The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells. PLoS ONE 13(1): e0191682. //doi.org/10.1371/journal.pone.0191682](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Western-Blot-NBP2-27103-img0017.jpg)
![Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - Serum from trauma patients induces intrinsic apoptosis resistance by up-regulating AAT expression in neutrophils. AAT protein expression was analyzed in neutrophils after 18 h of culture by western blot analysis. Relative expression was quantified vs. GAPDH expression. One representative blot of five independent experiments is depicted. **p<0.01 (one-way ANOVA with Newman keuls post-hoc test). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0177450), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Western-Blot-NBP2-27103-img0018.jpg)
![Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - WB detection of GAPDH protein (theoretical molecular weight 36 kDa) in lysates of Mouse cell lines (A) NIH 3T3 (B) RAW 264.7 using GAPDH antibody (clone 13H12) at a concentration of 0.25 ug/ml.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Western-Blot-NBP2-27103-img0005.jpg)
![Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - analysis of GAPDH in HeLa and HEK 293 cells (25ug/lane) using anti-GAPDH antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Western-Blot-NBP2-27103-img0014.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of normal human stomach tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Immunohistochemistry-Paraffin-NBP2-27103-img0007.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of human colon tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Immunohistochemistry-Paraffin-NBP2-27103-img0009.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Immunohistochemistry-Paraffin-NBP2-27103-img0010.jpg)
![Simple Western: GAPDH Antibody (13H12) [NBP2-27103] - GAPDH/G3PDH Antibody (13H12) [NBP2-27103] - Simple Western lane view shows a specific band for GAPDH in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GAPDH-Antibody-13H12-Simple-Western-NBP2-27103-img0015.jpg)
![Western Blot: GAPDH Antibody (13H12) - BSA Free [NBP2-27103] - Effect of AAT on Mcl-1 phosphorylation, the activity of MAP kinases & caspases.Neutrophils from healthy volunteers (2.5 × 106/ml) were cultured in medium supplemented with patient serum (3 mg protein/ml; 1%) & those containing low levels of AAT (AAT-reduce serum; 1%) in the presence of STS (0.2 μM). After 3 h, the expression of pMcl-1 (A, n = 7), pAkt (B, n = 8) & pERK1/2 (C, n = 10) were analyzed by western blot. Expression levels of the phosphorylated proteins were normalized to that of the unphosphorylated forms. GAPDH was used as loading control. One representative blot is displayed. *p<0.05; **p<0.01; n.s. = not significant. D. After 4 h of incubation the activities of caspase-9 & caspase-3/-7 were quantified. Results are presented as means ± SEM of eight independent experiments. No significant differences were found (one-way ANOVA with Newman keuls post-hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28493974), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-27103_mouse-monoclonal-gapdh-antibody-13h12-imgenex-img-6665a-25-31020241612222.jpg)
![Western Blot: GAPDH Antibody (13H12) - BSA Free [NBP2-27103] - Effect of AAT on Mcl-1 phosphorylation, the activity of MAP kinases & caspases.Neutrophils from healthy volunteers (2.5 × 106/ml) were cultured in medium supplemented with patient serum (3 mg protein/ml; 1%) & those containing low levels of AAT (AAT-reduce serum; 1%) in the presence of STS (0.2 μM). After 3 h, the expression of pMcl-1 (A, n = 7), pAkt (B, n = 8) & pERK1/2 (C, n = 10) were analyzed by western blot. Expression levels of the phosphorylated proteins were normalized to that of the unphosphorylated forms. GAPDH was used as loading control. One representative blot is displayed. *p<0.05; **p<0.01; n.s. = not significant. D. After 4 h of incubation the activities of caspase-9 & caspase-3/-7 were quantified. Results are presented as means ± SEM of eight independent experiments. No significant differences were found (one-way ANOVA with Newman keuls post-hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28493974), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-27103_mouse-monoclonal-gapdh-antibody-13h12-imgenex-img-6665a-25-310202416114762.jpg)
![Western Blot: GAPDH Antibody (13H12) - BSA Free [NBP2-27103] - Effect of AAT on Mcl-1 phosphorylation, the activity of MAP kinases & caspases.Neutrophils from healthy volunteers (2.5 × 106/ml) were cultured in medium supplemented with patient serum (3 mg protein/ml; 1%) & those containing low levels of AAT (AAT-reduce serum; 1%) in the presence of STS (0.2 μM). After 3 h, the expression of pMcl-1 (A, n = 7), pAkt (B, n = 8) & pERK1/2 (C, n = 10) were analyzed by western blot. Expression levels of the phosphorylated proteins were normalized to that of the unphosphorylated forms. GAPDH was used as loading control. One representative blot is displayed. *p<0.05; **p<0.01; n.s. = not significant. D. After 4 h of incubation the activities of caspase-9 & caspase-3/-7 were quantified. Results are presented as means ± SEM of eight independent experiments. No significant differences were found (one-way ANOVA with Newman keuls post-hoc test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28493974), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-27103_mouse-monoclonal-gapdh-antibody-13h12-imgenex-img-6665a-25-310202416121949.jpg)
![Western Blot: CD133 Antibody - BSA Free [NB120-16518] - Predicted band size : 97 kDa recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation.](http://images.novusbio.com/fullsize/CD133-Antibody---BSA-Free-Western-Blot-NB120-16518-img0003.jpg "Western Blot: CD133 Antibody - BSA Free [NB120-16518] - Predicted band size : 97 kDa recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation.")
![Immunocytochemistry/Immunofluorescence: CD133 Antibody - BSA Free [NB120-16518] - Characterization of Scattered tubular-like cells (STCs) isolated from pig kidneys. A) Representative immunofluorescence staining (original magnification 40X) for the STC surface markers CD133 NB120-16518G (red) and CD24 (green) in isolated swine STCs. B) Flow cytometry analysis of isolated STCs showing that 97% of cells expressed CD133, 95% CD24, and 98% were double-positive for CD133 and CD24 (n = 3 each). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31614781/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD133 Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NB120-16518-img0008.jpg)
![Immunohistochemistry: CD133 Antibody - BSA Free [NB120-16518] - Immunostaining for CD133 (NB120-16518) in normal ovarian and tumor tissue sections. Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed. White scale bar?=?50 um; blue scale bar?=?10 um. Secondary antibody employed was conjugated with Alexa fluor 568 and tissue sections were counterstained with nucleus specific dye DAPI. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30121075/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD133-Antibody---BSA-Free-Immunohistochemistry-NB120-16518-img0007.jpg)
![Flow Cytometry: CD133 Antibody - BSA Free [NB120-16518] - Flow Cytometry: CD133 Antibody [DyLight 488] [NB120-16518G] - Flow Cytometry: CD133 Antibody (DyLight488) [NB120-16518G] - Rat bone marrow cells were stained with CD133 (1:100) antibody (20 minutes at 4C), fixed, and then analyzed. Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/CD133-Antibody---BSA-Free-Flow-Cytometry-NB120-16518-img0006.jpg)
![Chromatin Immunoprecipitation: CD133 Antibody - BSA Free [NB120-16518] - [NB 120-16518] - Staining CD133 in Bone Marrow Mononuclear Cells by Immunofluorescence.](http://images.novusbio.com/fullsize/CD133-Antibody---BSA-Free-Chromatin-Immunoprecipitation-NB120-16518-img0005.jpg)
![Immunohistochemistry-Paraffin: CD133 Antibody - BSA Free [NB120-16518] - Staining of CD133 in human hepatocarcinoma (HC) tissue. NB120-16518 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.](http://images.novusbio.com/fullsize/CD133-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB120-16518-img0002.jpg)
![Immunostaining for CD133 in normal ovarian and tumor tissue sections: [A] Mouse monoclonal anti-CD133 antibody was localized in both OSE (A, B) and ovarian cortex (C, D) by immunohistochemistry. Region between dotted boxes in A, C is magnified in B, D respectively. Polar staining of CD133 is obvious in OSE layer especially in NO, BL and HG ovaries. BL ovaries exhibit multi-layered OSE. Cortex comprised of CD133+ cells arranged in clusters with elongated/spindle shaped morphology in NO and BN ovaries. BL ovarian cortex harbours single spherical cell clusters distributed throughout. HG comprised more of large CD133+ cells in OSE and few clusters in the cortex per field focussed. Insets include magnified images of cells from different fields. Scale bar = 100 μm (A, C) and 25 μm (B, D) respectively. [B] Immunofluorescence staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed. White scale bar = 50 μm; blue scale bar = 10 μm. Secondary antibody employed was conjugated with Alexa fluor 568 and tissue sections were counterstained with nucleus specific dye DAPI Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30121075), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-16518_rabbit-polyclonal-cd133-antibody-immunocytochemistry-immunofluorescence-13220261942455.jpg)
![Western Blot: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of 25 ug of mouse brain (lane 1) and rat brain (lane 2) cell lysates.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Western-Blot-NB300-556-img0020.jpg "Western Blot: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of 25 ug of mouse brain (lane 1) and rat brain (lane 2) cell lysates.")
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Control immunolocalization of plasma membrane AMPA receptor subunits (external epitopes) in hippocampal neuronal cultures. H) Immunolabeling of non-permeabilized neuronal cultures with ani-GluA1 (green). (I) Immunolabeling with the postsynaptic marker anti-PSD95 [66] after permeabilization of neuronal cultures. (J) Merging of (H) and (I) showing postsynaptic labeling of GluA1. Scale bar (valid for all images): 10 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0140868), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Immunocytochemistry-Immunofluorescence-NB300-556-img0025.jpg)
![Immunohistochemistry-Paraffin: PSD-95 Antibody (6G6-1C9) [NB300-556] - Confocal images of dissociated hippocampal cultures. Double labeling with anti-syntaxin-1 Double labeling with antibodies against syntaxin-1 (D) and synaptophysin (E) indicates that syntaxin-1 is colocalized with presynaptic marker (F). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fnmol.2016.00010/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Immunohistochemistry-Paraffin-NB300-556-img0024.jpg)
![Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - An intracellular stain was performed on HeLa cells with PSD-95 Antibody (6G6-1C9) NB300-556AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Flow-Cytometry-NB300-556-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) shows staining in HeLa cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Immunocytochemistry-Immunofluorescence-NB300-556-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) shows staining in U251 glioma cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Immunocytochemistry-Immunofluorescence-NB300-556-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) shows staining in C6 glioma cells. PSD95 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PSD95 at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Immunocytochemistry-Immunofluorescence-NB300-556-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of Post Synaptic Density 95kD protein (PSD95, green) in cultured primary cortical neurons. Primary cortical neurons are isolated and cultured using the Primary Neuron Isolation Kit. At day 28, neurons were fixed with 4% paraformaldehyde, permeablilized with 0.1% triton X-100 in HBSS for 10 minutes at room temperature, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were probed with a PSD95 monoclonal antibody at a dilution of 1:500 for 2 hours at room temperature or overnight at 4C, washed with HBSS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Immunocytochemistry-Immunofluorescence-NB300-556-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Mixed 3 week old rat cortical cultures (21 DIV) were labelled with anti-PSD95 (1:650) and visualised with anti-mouse Alexa647 (1:2000). The antibody labels small punctae along neuronal processes as expected. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Immunocytochemistry-Immunofluorescence-NB300-556-img0021.jpg)
![Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in Neuro-2a cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Flow-Cytometry-NB300-556-img0012.jpg)
![Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in SH-SY5Y cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Flow-Cytometry-NB300-556-img0013.jpg)
![Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in U87-MG cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PSD-95-Antibody-6G6-1C9-Flow-Cytometry-NB300-556-img0014.jpg)
![Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] - Confocal images of dissociated hippocampal cultures. Double labeling with anti-syntaxin-1 (A) & anti-Tuj1 (B) demonstrates that syntaxin-1 is located along the dendrites & gives characteristic punctate labeling (C). Double labeling with antibodies against syntaxin-1 (D) & synaptophysin (E) indicates that syntaxin-1 is colocalized with presynaptic marker (F). Double labeling for syntaxin-1 (G) & PSD-95 (H) shows partial colocalization postsynaptic (I). Scale bars: 10 μm. (J–L) Western blot analysis of synaptosomes (Syn), synaptic cytosolic fraction (Cy), active zone (AZ) & post synaptic density (PSD). Labeled with anti-synaptophysin (J) anti-PSD95 (K) & anti-syntaxin-1 (L). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fnmol.2016.00010/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-556_mouse-monoclonal-psd-95-antibody-6g6-1c9-310202415392516.jpg)
![Western Blot: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] - Confocal images of dissociated hippocampal cultures. Double labeling with anti-syntaxin-1 (A) & anti-Tuj1 (B) demonstrates that syntaxin-1 is located along the dendrites & gives characteristic punctate labeling (C). Double labeling with antibodies against syntaxin-1 (D) & synaptophysin (E) indicates that syntaxin-1 is colocalized with presynaptic marker (F). Double labeling for syntaxin-1 (G) & PSD-95 (H) shows partial colocalization postsynaptic (I). Scale bars: 10 μm. (J–L) Western blot analysis of synaptosomes (Syn), synaptic cytosolic fraction (Cy), active zone (AZ) & post synaptic density (PSD). Labeled with anti-synaptophysin (J) anti-PSD95 (K) & anti-syntaxin-1 (L). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fnmol.2016.00010/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-556_mouse-monoclonal-psd-95-antibody-6g6-1c9-310202415334924.jpg)
![Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] - Confocal images of dissociated hippocampal cultures.Double labeling with anti-TUJ1 (A) & anti-VAMP2 (B) demonstrates that VAMP2 is located along the dendrites & gives characteristic punctate labeling (C). Double labeling with antibodies against synaptophysin (P38) (D) & VAMP2 (E) indicates that VAMP2 is colocalized with the presynaptic marker (F). Double labeling for PSD-95 (G) & VAMP2 (H) shows partial colocalization postsynaptic (I). Small, high-resolution pictures of single synapses from (F) & (I) are shown in the right part of these images, respectively. Scale bar: 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26488171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-556_mouse-monoclonal-psd-95-antibody-6g6-1c9-310202415395948.jpg)
![Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) - BSA Free [NB300-556] - Control immunolocalization of plasma membrane AMPA receptor subunits (external epitopes) in hippocampal neuronal cultures.(A) Beta-tubulin (green, anti-TuJ1) labeling of dendrite, after anti-GluA1 external epitope [66] labeling & subsequent plasma membrane permeabilization. Note punctate GluA1 [66] labeling along the dendrite. (B) Synaptophysin (P38, green) & GluA1 [66]. (C) Synaptophysin (green) & GluA2 [66]. (D) Labeling with anti-GluA1. (E) Labeling with anti-GluA1, but the antibody was preincubated with the peptide antigen before staining. (F) Labeling with anti-GluA2. (G) Labeling with anti-GluA2, but the antibody was preincubated with the peptide antigen before staining. (H) Immunolabeling of non-permeabilized neuronal cultures with ani-GluA1 (green). (I) Immunolabeling with the postsynaptic marker anti-PSD95 [66] after permeabilization of neuronal cultures. (J) Merging of (H) & (I) showing postsynaptic labeling of GluA1. Scale bar (in A, valid for all images): 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26488171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-556_mouse-monoclonal-psd-95-antibody-6g6-1c9-310202416205115.jpg)
![Western Blot: CD11b/c Antibody - BSA Free [NB110-40766] - Analysis of CD11b/c in Raw 264.7 whole cell lysate.](http://images.novusbio.com/fullsize/CD11b-c-Antibody---BSA-Free-Western-Blot-NB110-40766-img0002.jpg "Western Blot: CD11b/c Antibody - BSA Free [NB110-40766] - Analysis of CD11b/c in Raw 264.7 whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: CD11b/c Antibody - BSA Free [NB110-40766] - CD11 antibody was tested in Raw264.7 cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD11b-c-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-40766-img0006.jpg)
![Immunohistochemistry-Frozen: CD11b/c Antibody - BSA Free [NB110-40766] - 10 um Cryosection of murine acute brain slices stained with CD11b/c antibody (1:300 = 3.3 ug/ml) and Alexa Fluor 555 donkey anti rabbit IgG. Antibody shows specific staining, but the background is quite heavy. Image from verified customer review.](http://images.novusbio.com/fullsize/CD11b-c-Antibody---BSA-Free-Immunohistochemistry-Frozen-NB110-40766-img0009.jpg)
![Flow Cytometry: CD11b/c Antibody - BSA Free [NB110-40766] - A surface stain was performed on RAW 264.7 with NB110-40766 and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550.](http://images.novusbio.com/fullsize/CD11b-c-Antibody---BSA-Free-Flow-Cytometry-NB110-40766-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CD11b/c Antibody - BSA Free [NB110-40766] - Effects of oltipraz on the expression of CD11b/c, phosphoinositide 3-kinase (PI3K), phosphorylated protein kinase B (p-Akt), and phosphorylated inhibitor of kB alpha (p-IkB alpha) in the spinal cord of the CCI-injured mice. The relative protein levels of (A) CD11b/c and (B) PI3K on the ipsilateral side of the spinal cord in the CCI-injured mice treated with oltipraz (OLT) or vehicle are represented. (C) Representative examples of blots for CD11b/c (160 kDa), PI3K (130 kDa), and GAPDH (37 kDa). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31234342/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD11b-c Antibody - BSA Free-Western Blot-NB110-40766-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CD11b/c Antibody - BSA Free [NB110-40766] - Phase contrast microscopy images of HFA and brain tumor cells and immunofluorescence images of cultured primary brain tumor cells, AA and HFA. HFA were derived from three different fetal brains. HFA from an 18-week- old fetus (passaged once) - GFAP (DAKO) merged with DAPI and CD68 (zoomed: 2.1x magnification); GFAP (green and red) was used as an astrocyte marker and CD68 (red) and CD11b (green) were used as markers for microglia. Nuclei indicated by DAPI (blue) in all images. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0112945), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD11b-c-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-40766-img0010.jpg)
![Immunohistochemistry: CD11b/c Antibody - BSA Free [NB110-40766] - Staining of human bone marrow, myeloid precursors.](http://images.novusbio.com/fullsize/CD11b-c-Antibody---BSA-Free-Immunohistochemistry-NB110-40766-img0007.jpg)
![Western Blot: CD11b/c Antibody - BSA Free [NB110-40766] - Effects of oltipraz on the expression of CD11b/c, phosphoinositide 3-kinase (PI3K), phosphorylated protein kinase B (p-Akt), & phosphorylated inhibitor of kappa B alpha (p-IkB alpha ) in the spinal cord of the CCI-injured mice. The relative protein levels of (A) CD11b/c, (B) PI3K, (D) p-Akt, & (E) p-IKB alpha on the ipsilateral side of the spinal cord in the CCI-injured mice treated with oltipraz (OLT) or vehicle are represented. The sham-operated mice (SHAM) treated with vehicle were used as controls. (C) Representative examples of blots for CD11b/c (160 kDa), PI3K (130 kDa), & GAPDH (37 kDa), & (F) for p-Akt (60 kDa), Akt (60 kDa), p-IKB alpha (40 kDa) & IKB alpha (40 kDa). CD11b/c & PI3K are expressed relative to GAPDH levels whereas phosphorylated proteins are expressed relative to their corresponding total proteins. In all panels, * denotes significant differences vs. sham-operated mice treated with vehicle (p < 0.05; one-way ANOVA followed by the SNK test). Results are presented as the mean ± SEM; n = 5 samples per experimental group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31234342), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40766_rabbit-polyclonal-cd11b-c-antibody-310202416235533.jpg)
![Western Blot: CD11b/c Antibody - BSA Free [NB110-40766] - Effects of oltipraz on the expression of CD11b/c, PI3K, p-Akt, & p-IkB alpha in the prefrontal cortex of the CCI-injured mice. The relative protein levels of (A) CD11b/c, (B) PI3K, (D) p-Akt, & (E) p-IKB alpha in the prefrontal cortex of the CCI-injured mice treated with oltipraz (OLT) or vehicle. The sham-operated mice (SHAM) treated with vehicle were used as controls. Representative examples of blots for (C) CD11b/c (160 kDa), PI3K (130 kDa) & GAPDH (37 kDa), & for (F) p-Akt (60 kDa), Akt (60 kDa), p-IKB alpha (40 kDa) & IKB alpha (40 kDa). CD11b/c & PI3K are expressed relative to GAPDH levels whereas phosphorylated proteins are expressed relative to their corresponding total proteins. In all panels, * denotes significant differences vs. sham-operated mice treated with vehicle (p < 0.05; one-way ANOVA followed by the SNK test). Results are presented as the mean ± SEM; n = 4-–5 samples per experimental group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31234342), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40766_rabbit-polyclonal-cd11b-c-antibody-310202416235518.jpg)
![Western Blot: CD11b/c Antibody - BSA Free [NB110-40766] - Effects of oltipraz on the expression of CD11b/c, PI3K, p-Akt, & p-IkB alpha in the hippocampus of the CCI-injured mice. The relative protein levels of (A) CD11b/c, (B) PI3K, (D) p-Akt, & (E) p-IKB alpha in the hippocampus of the CCI-injured mice treated with oltipraz (OLT) or vehicle. The sham-operated mice (SHAM) treated with vehicle were used as controls. Representative examples of blots for (C) CD11b/c (160 kDa), PI3K (130 kDa), & GAPDH (37 kDa); & (F) for p-Akt (60 kDa), Akt (60 kDa), p-IKB alpha (40 kDa), & IKB alpha (40 kDa). CD11b/c & PI3K are expressed relative to GAPDH levels whereas phosphorylated proteins are expressed relative to their corresponding total proteins. In all panels, * denotes significant differences vs. sham-operated mice treated with vehicle (p < 0.05; one-way ANOVA followed by the SNK test). Results are presented as the mean ± SEM; n = 5 samples per experimental group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31234342), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40766_rabbit-polyclonal-cd11b-c-antibody-310202416235538.jpg)
![Immunocytochemistry/ Immunofluorescence: CD11b/c Antibody - BSA Free [NB110-40766] - Phase contrast microscopy images of HFA & brain tumor cells & immunofluorescence images of cultured primary brain tumor cells, AA & HFA.HFA were derived from three different foetal brains. (A) 18-week foetus (10× magnification); (B) 19-week foetus, first week in culture (10× magnification); (C) 19-week foetus, day 5 in culture (20× magnification); (D) Primary GBM after 2 trypsinisations (20x); (E) Recurrent GBM (20x); (F) Secondary GBM after 3 trypsinisations (10x); (G) OII 14 days in culture (10x); (H) AII 13 days in culture (10x); (I) Negative control for NGS merged with DAPI; (J) Negative control for secondary antibodies merged with DAPI; (K) GBM cells derived from one patient – GFAP (DAKO) merged with DAPI & CD68 (zoomed: 2.4× magnification); (L) primary brain tumor cells from one patient (male, 40 years old) (passaged three times) - GFAP (Novocastra) merged with DAPI; (K & L) GFAP (green) was used as a GBM marker & CD68 (red) was used as a marker for microglia. (M) AA from one Female, 61 years old; (passaged twice) - GFAP (Novocastra) merged with DAPI; (N) AA from one male 40 years old; (passaged once) - GFAP (Sigma) merged with DAPI & CD68 (Abcam); (M & N) GFAP (green) was used as an astrocyte marker & CD68 (red) & CD11b (green) were used as markers for microglia; (O) HFA from one 17-week- old foetus (passaged once) - GFAP (Novocastra) merged with DAPI & CD11b (Novus); (P) HFA from an 18-week- old foetus (passaged once) - GFAP (DAKO) merged with DAPI & CD68 (zoomed: 2.1× magnification); (O & P) GFAP (green & red) was used as an astrocyte marker & CD68 (red) & CD11b (green) were used as markers for microglia. Nuclei indicated by DAPI (blue) in all images. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0112945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40766_rabbit-polyclonal-cd11b-c-antibody-310202416235543.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Immunostaining of SCP3/SYCP3 (NB300-231) and SCP1 (NB300-229) on adult Cxxc1 het and CKO chromosome spreads. Green, SCP3/SYCP3; orange, SCP1. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-231-img0014.jpg "Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Immunostaining of SCP3/SYCP3 (NB300-231) and SCP1 (NB300-229) on adult Cxxc1 het and CKO chromosome spreads. Green, SCP3/SYCP3; orange, SCP1. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547/) licensed under a CC-BY license.")
![Immunohistochemistry: SCP3/SYCP3 Antibody [NB300-231] - Punctate staining of murine SCP3 in mouse ovary using NB300-231.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunohistochemistry-NB300-231-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Distinct forms of ubiquitin regulation by RNF8 and SCML2. (C-F) Immunostaining on meiotic chromosome spreads of FK2 and SYCP3 antibodies. Dotted circles are sex chromosomes and Scale bar are 10 micrometers. At least 30 spermatocytes from each substage are shown from at least 3 independent mice per mouse model. Immunostaining intensity is qualified by densitometry across the indicated path and plotted in a relative intensity of 0-1 (normalized among stages). Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A-D) Immunostaining of D27C4 and SYCP3 on meiotic chromosome spreads. Dotted circles indicate sex chromosomes and scale bar is 10 micrometers. At least 30 spermatocytes from each substage are shown from at least 3 independent mice per mouse model. Immunostaining intensity is qualified by densitometry across the indicated path and plotted in a relative intensity of 0-1 (normalized among stages).Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A) Immunostaining of D27C4 and SYCP3 on wild-type meiotic chromosome spreads. Representative images are shown for each category through the early pachytene stage for spermatocytes from 9 independent mice. (C) Immunostaining of SYCP3 and D27C4 on Mdc1-KO meiotic chromosome spreads. Representative images are shown through the early pachytene stage for spermatocytes from 3 independent Mdc1-KO mice. (A and C) Dotted circles indicate sex chromosomes and scale bar is 10 micrometers. Immunostaining intensity is qualified by densitometry across the indicated path and plotted in a relative intensity of 0-1 (normalized among stages).Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A-D) Immunostaining of E6C5 and SYCP3 on meiotic chromosome spreads. Dotted circles indicate sex chromosomes and scale bar is 10 micrometers. At least 30 spermatocytes from each substage are shown from at least 3 independent mice per mouse model.Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A, B) Immunostaining of SCML2 and SYCP3 on meiotic chromosome spreads. Dotted circles indicate sex chromosomes and scale bar is 10 micrometers. At least 30 spermatocytes from each substage are shown from at least 3 independent mice per mouse model.Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A-D) Immunostaining of H3K4me2 and SYCP3 on meiotic chromosome spreads. Dotted circles: sex chromosomes. (E) Regulation of H3K4me2. (F, G) Immunostaining of H3K4me2 and SYCP3 on wild-type and Scml2-KO meiotic chromosome spreads. All: Dotted circles indicate sex chromosomes and scale bar is 10 micrometers. Immunostaining intensity is qualified by densitometry across the indicated path and plotted in a relative intensity of 0-1 (normalized among stages).Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - (A-D) Immunostaining of H3K27ac and SYCP3 on meiotic chromosome spreads. At least 30 spermatocytes from each substage are shown from at least 3 independent mice per mouse model. Immunostaining intensity is qualified by densitometry across the indicated path and plotted in a relative intensity of 0-1 (normalized among stages). (E) Model of the regulation of H3K27ac. (F) Immunostaining of SYCP3 and H3K27ac on wild-type and Rnf8-KO round spermatids. At least 30 round spermatids shown from at least 3 independent mice. All: Dotted circles indicate sex chromosomes and scale bar is 10 micrometers.Citation: Adams SR, Maezawa S, Alavattam KG, Abe H, Sakashita A, Shroder M, et al. (2018) RNF8 and SCML2 cooperate to regulate ubiquitination and H3K27 acetylation for escape gene activation on the sex chromosomes. PLoS Genet 14(2): e1007233. //doi.org/10.1371/journal.pgen.1007233](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-ICC-IF-Negative-NB300-231-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Mouse spermatocytes stained with biotin-conjugated anti-SYCP3 detected with streptavidin-Alexa Fluor 594 (left panel), streptavidin Alexa Fluor 488 (right panel). Image from verified customer review.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-231-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - SCP3 labeled in mouse pachytene preparation (red), using NB 300-231. CDK2 staining, near teleomeres, is also present (green).](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-231-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - CXXC1 is expressed in the spermatocytes in the presence or absence of PRDM9.(A) Immunofluorescence staining for CXXC1 & PRDM9 in adult B6, 14-dpp B6 & 14-dpp Prdm9-/- seminiferous tubule cross sections. Green, CXXC1; red, PRDM9; blue, DAPI. SC, Sertoli cell; Z, zygonema; P, pachynema; RS, round spermatid. Scale bars, first 4 columns: 50 μm, last column: 10 μm. (B) Immunofluorescence staining for CXXC1 & PRDM9 on chromosome spreads from adult B6. Green, SYCP3; orange, PRDM9; magenta, CXXC1. Scale bars, 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202415392592.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - RNF8 is required for polyubiquitination of the sex chromosomes: Immunostaining with E6C5 antibody, which recognizes polyubiquitination.(A-D) Immunostaining of SYCP3 & E6C5 on meiotic chromosome spreads. Dotted circles: sex chromosomes. Scale bar: 10 μm. Representative images are shown for at least 30 spermatocytes from each substage, from at least 3 independent mice per mouse model. (E) Schematic of ubiquitin targets recognized by the E6C5 antibody in each mouse model. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007233), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202415291958.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - RNF8-dependent H2AK119ub is removed by SCML2: Immunostaining with D27C4 antibody, which recognizes H2AK119ub.(A-D) Immunostaining of SYCP3 & D27C4 (H2AK119ub) on meiotic chromosome spreads. Dotted circles: sex chromosomes. Scale bar: 10 μm. Representative images are shown for at least 30 spermatocytes from each substage, from at least 3 independent mice per mouse model. The intensity of immunostaining is quantified by densitometry across the indicated path (‘ to”) & plotted in a relative intensity range of 0–1, which is normalized among all images in this figure, Fig 3 & S2 Fig. (E) Updated model of distinct forms of regulation of ubiquitination by RNF8 & SCML2, including RNF8-dependent H2AK119ub, which is removed by SCML2 based on data in this figure. (F) Schematic of ubiquitin targets recognized by the D27C4 (H2AK119ub) antibody in each mouse model. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007233), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202416205194.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Distinct forms of ubiquitin regulation by RNF8 & SCML2: Immunostaining with FK2 antibody, which recognizes both mono- & polyubiquitinated conjugates.(A) Schematic of spermatogenesis. (B) Model of distinct forms of regulation of ubiquitination by RNF8 & SCML2. (C-F) Immunostaining of SYCP3 & FK2 on meiotic chromosome spreads. Dotted circles: sex chromosomes. Scale bar: 10 μm. Representative images are shown for at least 30 spermatocytes from each substage, from at least 3 independent mice per mouse model. The intensity of immunostaining is quantified by densitometry across the indicated path (‘ to”) & plotted in a relative intensity range of 0–1, which is normalized among the samples at the same stage. (G) Schematic of ubiquitin targets recognized by the FK2 antibody in each mouse model. A pink circle denotes RNF8-dependent ubiquitination & an orange circle denotes events mediated by a different E3 ubiquitin ligase. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007233), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202416212367.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - No major meiotic DSB repair or chromosome synapsis defects are observed in Cxxc1 CKO testis.(A) Immunostaining of SYCP3 & gamma H2AX on adult Cxxc1 het & CKO chromosome spreads. Green, SYCP3; magenta, gamma H2AX. Scale bar, 10 μm. (B) Spermatocyte stage proportion in adult Cxxc1 het (n = 1,062 from two individuals) & CKO (n = 1,105 from two individuals) spermatocytes based on SYCP3/SYCP1/ gamma H2AX staining. p = 0.7 by Chi-square test. (C) Immunostaining of SYCP3 & SYCP1 on adult Cxxc1 het & CKO chromosome spreads. Green, SYCP3; orange, SYCP1. Scale bar, 10 μm. (D) Crossover number measured by MLH1 staining on chromosome spreads of adult Cxxc1 het & CKO spermatocytes. Left, magenta, SYCP3; green, MLH1. Scale bar, 10 μm. Right, number of MLH1 foci per late pachynema in Cxxc1 het (n = 32 from two individuals) & CKO (n = 33 from two individuals). Bars represent mean ± SD. p = 0.4 by Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-3102024162188.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - Meiotic spreads show deletion of Dicer1 disrupts progression of meiosis I.(A) It was possible to identify the 5 sub-stages of meiosis I by combining the reactivity patterns of two antibodies against synaptonemal complex protein 3 (SYCP3) & gamma H2AX. SYCP3 is a protein essential for synapsis of homologous chromosomes & gamma H2AX localizes to double-strand breaks & XY bodies during meiosis. A total of 200 spreads were counted in testis cell preparations from WT & GCKO mice at P22. (B) Chi square analysis shows GCKO testes contained significantly higher numbers of germ cell spreads at the leptotene & zygotene stages of meiosis I & fewer spreads at pachytene, diplotene & metaphase I stages (P<0.001), suggesting that the loss of Dicer1 lead to disruptions in progression through meiosis I. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23056286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202416212316.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - PRDM9 expression & catalytic function are not impaired in Cxxc1 CKO.(A) Immunostaining of PRDM9 shows unchanged pattern in adult CKO seminiferous tubules compared to the heterozygous control. Red, PRDM9; grey, DAPI. Scale bar, 20 μm. (B) PRDM9 & SYCP3 expression patterns are not changed in CXXC1 CKO chromosome spreads compared to the heterozygous control. Co-immunostaining of CXXC1 & PRDM9 on chromosome spreads from adult Cxxc1 het & CKO mice. Green, SYCP3; orange, PRDM9; magenta, CXXC1. First 4 rows, het control; last 4 rows, Cxxc1 CKO. Scale bar, 10 μm. (C) Meiosis progression occurs normally in Cxxc1 CKO testes. Immunostaining of H3K4me3 in adult Cxxc1 het & CKO chromosome spreads. Green, SYCP3; magenta, H3K4me3. Scale bar, 10 μm. (D) Testis-specific gene expression is not changed in Cxxc1 CKO testes. H3K4me3 ChIP-qPCR with chromatin isolated form Cxxc1 het & CKO mice. Promoter regions form Actinb & Sycp3, Dom2 hotspots PbxI & Fcgr4 were amplified. Cst hotspot HlxI was used as a negative control. Bars present mean ± SD of three biological replicates. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202416205179.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP3/SYCP3 Antibody [NB300-231] - DSB number is not affected in Cxxc1 CKO.The DSB number was determined by three markers reflecting different stages of their processing. (A) DMC1 staining on Cxxc1 control & CKO chromosome spread. Bottom panels, distribution plot of DMC1 foci in early zygotene (n = 22 in het, n = 34 in CKO), late zygotene (n = 29 in het, n = 36 in CKO) & pachytene (n = 36 in het, n = 26 in CKO) spermatocytes. (B) RAD51 staining on Cxxc1 control & CKO chromosome spread. Bottom panels, distribution plot of RAD51 foci in early zygotene (n = 22 in het, n = 13 in CKO), late zygotene (n = 24 in het, n = 11 in CKO) & pachytene (n = 52 in het, n = 36 in CKO) spermatocytes. (C) RPA staining on Cxxc1 control & CKO chromosome spread. Bottom panels, distribution plot of RPA foci in early zygotene (n = 35 in het, n = 43 in CKO), late zygotene (n = 33 in het, n = 28 in CKO) & pachytene (n = 89 in het, n = 92 in CKO) spermatocytes. For A-C, two individuals per genotype were measured. Bars represent mean ± SD. Scale bar, 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-231_rabbit-polyclonal-scp3-sycp3-antibody-310202416203720.jpg)
![Immunohistochemistry: GAP-43 Antibody [NBP1-41123] - Coronal sections of the developing (A-H) eye at (A-D) E14.5, (E-H) E17.5. were immunostained for (A-H) EVA1C and (A-H) GAP43. All sections were counterstained with DAPI. EVA1C was strongly co-expressed with GAP43 by retinal cell ganglionic axons (A-H) as they exit the retina and form the optic nerve (arrowheads). Scale bars represent 100 uM. PLoS One. 2013 Sep 9;8(9):e74115. doi: 10.1371/journal.pone.0074115.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunohistochemistry-NBP1-41123-img0009.jpg "Immunohistochemistry: GAP-43 Antibody [NBP1-41123] - Coronal sections of the developing (A-H) eye at (A-D) E14.5, (E-H) E17.5. were immunostained for (A-H) EVA1C and (A-H) GAP43. All sections were counterstained with DAPI. EVA1C was strongly co-expressed with GAP43 by retinal cell ganglionic axons (A-H) as they exit the retina and form the optic nerve (arrowheads). Scale bars represent 100 uM. PLoS One. 2013 Sep 9;8(9):e74115. doi: 10.1371/journal.pone.0074115.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP1-41123] - Staining of a mature retinal ganglion cell demonstrating axon outgrowth.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-41123-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: GAP-43 Antibody [NBP1-41123] - Longitudinal [11C]GV1-57 imaging monitors mature OSN regeneration. Immunoblotting of immature neurons (GAP-43), mature neurons (OMP), and sustentacular cells (REEP6) in septal OE tissue collected from animals 2 or 11 weeks after ZnSO4 or vehicle treatment. Red arrows indicate DVR values and the corresponding immunoblots of OE tissue collected from the same individual animals 11 weeks after treatment. Purple arrows indicate DVR values representative of [11C]GV1-57 imaging 2 weeks after treatment and corresponding immunoblots of OE tissue collected from a separate cohort of animals. n = 2 per group at 2 weeks after treatment; n = 1 per group at 11 weeks after treatment. Image collected and cropped by CiteAb from the following publication (//www.jci.org/articles/view/89162), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP1-41123-img0008.jpg)
![Western Blot: GAP-43 Antibody [NBP1-41123] - Analysis of GAP43 in human brain protein](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP1-41123-img0006.jpg)
![Western Blot: GAP-43 Antibody [NBP1-41123] - Total protein from Human, Mouse and Rat brain, and SHSY-5Y cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-GAP43 in 1% non-fat milk in TBST and detected with an anti-sheep HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP1-41123-img0007.jpg)
![Immunohistochemistry: Nestin Antibody (10C2) [NB300-266] - GFAP and Nestin immunoreactivity in retinal sections.Epifluorescent acquisition of p28 Reeler and WT retinas. (A-D) As compared to WT, both GFAP (red) and Nestin (green) immunoreactivities were highly visible in Reeler retinas (AB, GCL). Arrows point at yellow immunoreactivity in the white frame (B) indicating GFAP and Nestin co-expression in cells having long-filaments (activated Muller cells), as compared to wild type (arrowheads in A). Nuclei were DAPI counterstained (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212732) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunohistochemistry-NB300-266-img0020.jpg "Immunohistochemistry: Nestin Antibody (10C2) [NB300-266] - GFAP and Nestin immunoreactivity in retinal sections.Epifluorescent acquisition of p28 Reeler and WT retinas. (A-D) As compared to WT, both GFAP (red) and Nestin (green) immunoreactivities were highly visible in Reeler retinas (AB, GCL). Arrows point at yellow immunoreactivity in the white frame (B) indicating GFAP and Nestin co-expression in cells having long-filaments (activated Muller cells), as compared to wild type (arrowheads in A). Nuclei were DAPI counterstained (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212732) licensed under a CC-BY license.")
![Western Blot: Nestin Antibody (10C2) [NB300-266] - WB analysis of 5 ug lysate from (1) Rat's total brain tissue, and (2) human CNS progenitor cells using Nestin antibody (clone 10C2) at 1:1000 dilution. The antibody did not react with rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Western-Blot-NB300-266-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) [NB300-266] - Analysis of formalin fixed, 0.1% Triton X-100 permeabilized F9 cells (Mouse teratocarcinoma) using Nestin antibody (clone 10C2) at 1:50 dilution. The primary antibody was detected using Alexa Fluor 488 labeled donkey anti-mouse IgG (H+L) cross-adsorbed secondary antibody (green) and nuclei were counterstained with DAPI (blue). An isotype control set was inclubated with mouse IgG1 isotype control antibody and the above mentioned secondary antibody. Nestin antibody (clone 10C2) generated a specific membrane-cytoplasmic staining of Nestin while the isotype control samples did not show any staining under same assay conditions.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunocytochemistry-Immunofluorescence-NB300-266-img0015.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody (10C2) [NB300-266] - Nestin was detected in immersion fixed paraffin-embedded sections of human kidney using anti-human mouse monoclonal antibody (Catalog # NB300-266) at 1:500 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunohistochemistry-Paraffin-NB300-266-img0013.jpg)
![Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Nestin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Flow-Cytometry-NB300-266-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) [NB300-266] - Visualization of Nestin in PC-3 cells using NB300-266. Courtesy of Dolnick Lab, Roswell Park Cancer Institute.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunocytochemistry-Immunofluorescence-NB300-266-img0006.jpg)
![Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - An intracellular stain was performed on SH-SY5Y cells with Nestin [10C2] Antibody NB300-266C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Flow-Cytometry-NB300-266-img0017.jpg)
![Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - An intracellular stain was performed on HeLa cells with Nestin Antibody (10C2)NB300-266PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Flow-Cytometry-NB300-266-img0016.jpg)
![Immunohistochemistry: Nestin Antibody (10C2) - BSA Free [NB300-266] - Regorafenib inhibits tumour growth and vascularisation in xenograft mice. GC1 were implanted into the right forebrain of mice. The mice then received daily oral vehicle or regorafenib at 30 mg/kg for 45 days. Representative photographs of mice brain tumours treated with regorafenib (right panel) or without regorafenib (left panel) stained by hemalun-eosin (median panel) or with nestin antibody (upper and lower panel). The upper panel shows whole brains of mice stained with nestin antibody. The tumour areas were circled in blue, and each surface was measured in mm^2. The red rectangles indicate the zone of the tumour shown in median (hemalun-eosin staining) and lower (nestin IHC) panel. Scale bars, upper panel 2.5 mm, median and lower panel 100 um. The graph shows the tumour size in mm^2 (expressed as the mean +/- SEM of 4 mice). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35326702/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb300-266_mouse-monoclonal-nestin-antibody-10c2-20920231412574.jpg)
![Immunohistochemistry: Nestin Antibody (10C2) - BSA Free [NB300-266] - Regorafenib inhibits tumour growth & vascularisation in xenograft mice. GC1 were implanted into the right forebrain of mice. The mice then received daily oral vehicle or regorafenib at 30 mg/kg for 45 days. (A) Representative photographs of mice brain tumours treated with regorafenib (right panel) or without regorafenib (left panel) stained by hemalun–eosin (median panel) or with nestin antibody (upper & lower panel). The upper panel shows whole brains of mice stained with nestin antibody. The tumour areas were circled in blue, & each surface was measured in mm2. The red rectangles indicate the zone of the tumour shown in median (hemalun–eosin staining) & lower (nestin IHC) panel. Scale bars, upper panel 2.5 mm, median & lower panel 100 µm. The graph shows the tumour size in mm2 (expressed as the mean ± SEM of 4 mice). (B) Representative immunohistochemistry photographs of CD31+ vessels in brain tumour areas of 3 different mice treated with regorafenib (right panel) or without regorafenib (control, left panel). Arrows indicate CD31+ blood vessels. Scale bars, 20 µm. The graph shows the number of CD31+ vessels in tumours per mm2 (expressed as mean ± SEM of 4 mice). * p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35326702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-266_mouse-monoclonal-nestin-antibody-10c2-310202416212379.jpg)
