Nestin Antibody (10C2) [Alexa Fluor® 647]

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Product Details

Summary
Reactivity Hu, Mu(-), Rb(-), Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P
Clone
10C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647

Nestin Antibody (10C2) [Alexa Fluor® 647] Summary

Immunogen
A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681]
Localization
Cytoplasmic
Marker
Neural Stem Cell Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NES
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This Nestin (10C2) antibody is useful for Western blot, Immunofluorescence/Immunocytochemistry, Flow Cytometry, Immunohistochemistry on formaldehyde fixed cultured cells and formalin fixed paraffin-embedded tissue sections. In Western blot a doublet band is seen at ~220-240 kDa. The investigator should determine the optimal working dilution for a specific application.

Reactivity Notes

Human. It does not cross-react mouse, rat or rabbit.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Nestin Antibody (10C2) [Alexa Fluor® 647]

  • FLJ21841
  • NES
  • Nestin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nestin Antibody (NB300-266AF647) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 700 Labeled NB300-266AF700

Additional Nestin Products

Nestin NB300-266AF647

Bioinformatics Tool for Nestin Antibody (NB300-266AF647)

Discover related pathways, diseases and genes to Nestin Antibody (NB300-266AF647). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Nestin Antibody (NB300-266AF647)

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Pathways for Nestin Antibody (NB300-266AF647)

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PTMs for Nestin Antibody (NB300-266AF647)

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Research Areas for Nestin Antibody (NB300-266AF647)

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Blogs on Nestin.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including...  Read full blog post.

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Bioinformatics

Gene Symbol NES
Entrez