PSD-95 Antibody (6G6-1C9) [Alexa Fluor® 488]

Flow Cytometry: PSD-95 Antibody (6G6-1C9) [Alexa Fluor® 488] [NB300-556AF488] - An intracellular stain was performed on HeLa cells with PSD-95 Antibody (6G6-1C9) NB300-556AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

• Reactivity: Hu, Mu, Rt, In, Pm
• Applications: WB, Flow, ICC/IF, IHC, IP, B/N, ChIP
• Clone: 6G6-1C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

PSD-95 Antibody (6G6-1C9) [Alexa Fluor® 488] Summary

• Immunogen: Purified recombinant rat PSD-95.
• Localization: Cytoplasmic
• Marker: post-Synaptic Marker
• Specificity: Detects Post Synaptic Density 95 kDa (PSD-95) from rat tissues.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: DLG4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Chromatin Immunoprecipitation (ChIP)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot

Reactivity Notes

Invertebrate reactivity reported in scientific literature (PMID: 18182049). Primate reactivity reported in scientific literature (PMID: 20519524). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PSD-95 Antibody (6G6-1C9) [Alexa Fluor® 488]

• discs, large homolog 4 (Drosophila)
• disks large homolog 4
• DLG4
• FLJ97752
• FLJ98574
• Postsynaptic density protein 95
• post-synaptic density protein 95
• PSD95
• PSD-95
• PSD95SAP90SAP-90discs large homolog 4
• SAP90
• Synapse-associated protein 90
• Tax interaction protein 15

Background

Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSD-95 Antibody (NB300-556AF488)(2)

Publications using NB300-556AF488

• O'grady BJ, Balotin KM, Bosworth AM, McClatchey PM Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks BioRxiv (ICC/IF, Human)
• O'grady BJ, Balotin KM, Bosworth AM et al. Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks BioRxiv ACS Biomater Sci Eng 2020-12-15 [PMID: 33320550]

Bioinformatics

• Gene Symbol: DLG4
• Entrez:
  • 1742(Human)
  • 13385(Mouse)
  • 29495(Rat)