RBFOX3/NeuN Antibody [PE]

An intracellular stain was performed on U-2 OS human osteosarcoma cell line with Rabbit anti-RBFOX3/NeuN Affinity-purified Polyclonal Antibody conjugated to Phycoerythrin (Catalog # NBP1-77686PE, blue histogram) or matched control antibody (Catalog # NBP2-24983, orange histogram) at 2.5 µg/mL for 30 minutes at RT.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, Flow, ICC/IF, IHC, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: PE
• Concentration: 1.08 mg/ml

RBFOX3/NeuN Antibody [PE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: A synthetic peptide made to an internal region of the human RBFOX3/NeuN protein (within residues 20-100). [Swiss-Prot A6NFN3]
• Localization: Nucleus, Cytoplasm
• Marker: Neuronal Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBFOX3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP1-77686. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
• Theoretical MW: 33.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine and porcine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.08 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RBFOX3/NeuN Antibody [PE]

• anti-NeuN
• FLJ56884
• FLJ58356
• Fox-1 homolog C
• FOX3
• FOX-3
• hexaribonucleotide binding protein 3
• HRNBP3
• neuN antigen
• NeuN ihc
• NeuN immunohistochemistry
• NeuN rabbit
• NeuN staining
• NEUN
• neuronal nuclei antigen
• neuronal nuclei
• RBFOX3
• RNA binding protein fox-1 homolog 3
• RNA binding protein, fox-1 homolog (C. elegans) 3
• RNA binding protein, fox-1 homolog 3

Background

Rbfox3 (also known as Fox-3, Fox1 homolog C, Hrnbp3, and D11Bwg0517e) is one of three mammalian members of the RNA-binding protein Rbfox gene family, all of which are involved in regulating alternative RNA splicing. Originally identified as an epitope, NeuN is located within the N-terminal region (6-15 amino acids) of Rbfox3. Rbfox family members are highly conserved, having a single RNA recognition motif (RRM)-type RNA binding domain (RBD) near the center of the protein sequence. The RBD amino acid (aa) sequence is identical between members, Rbfox1 and Rbfox2, with 4 differences within the 77 aa domain of Rbfox3. The fox3 gene is comprised of 15 exons in humans, 11 exons in rat, with 3 variants in mouse containing 14 exons and another 3 variants containing 15 exons. The Rbfox3 protein is highly conserved across human, mouse, and rat with 98.9% sequence identity between mouse (isoform I) and rat and 83.9% sequence identity between mouse (isoform I) and human. The expression of Rbfox3/NeuN is restricted to the nervous system and has widely been used in stroke research. It is recognized as a marker of mature neuronal cell types in the spinal cord, cerebral cortex, hippocampus, dorsal thalamus, caudate/putamen, and cerebellum. NeuN has been shown to bind DNA and is predominantly nuclear. Differences in immunoreactivity have been reported between Rbfox3/NeuN subtypes in which the 46kDa is mainly found in the nucleus whereas the 48kDa form is primarily distributed in the cytoplasm (1,2).

Rbfox proteins participate in regulation of alternative splicing between family members as well as in autoregulation. While the breadth of brain and muscle specific targets for alternative splicing is well established for Rbfox proteins, those specific to Rbfox3/NeuN along with its alternative splicing mechanism are less understood. Rbfox3 has been shown to regulate neuronal differentiation by alternative splicing of Numb pre-mRNA and has a role in adult neurogenesis. Dysfunctional Rbfox3/NeuN has been associated with various neurological disorders such as neurodevelopmental delay, autism spectrum disorder, Benign rolandic epilepsy (BRE), and cognitive impairments (1,2).

References

1. Duan W, Zhang YP, Hou Z, Huang C, Zhu H, Zhang CQ, Yin Q. (2016) Novel Insights into NeuN: from Neuronal Marker to Splicing Regulator. Mol Neurobiol. 53(3):1637-1647. PMID: 25680637.

2. Su CH, D D, Tarn WY. (2018) Alternative Splicing in Neurogenesis and Brain Development. Front Mol Biosci. 5:12. PMID: 29484299

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RBFOX3/NeuN Antibody (NBP1-77686PE)(1)

Publication using NBP1-77686PE

• Zhou J, Zhang L, Peng J, Zhang X et Al. Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation Cell Metab 2024-06-21 [PMID: 38906140]

Bioinformatics

• Gene Symbol: RBFOX3