Apolipoprotein E/ApoE Antibody (WUE-4) [Alexa Fluor® 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
WUE-4
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 405

Apolipoprotein E/ApoE Antibody (WUE-4) [Alexa Fluor® 405] Summary

Immunogen
Purified human ApoE [UniProt# P02649]
Epitope
Residues 140-160 of purified human ApoE (LDLR receptor binding site).
Localization
Secreted
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
APOE
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
Application Notes
This ApoE antibody is useful for Western blot, ELISA, Immunohistochemistry and Immunoprecipitation. In Western blot a band is observed at ~36 kDa, representing the ApoE protein.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with human better than mouse. It does not appear to react with rat brain tissue.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Apolipoprotein E/ApoE Antibody (WUE-4) [Alexa Fluor® 405]

  • APOE
  • Apo-E
  • Apolipoprotein E
  • apolipoprotein E3
  • late onset)
  • MGC1571

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NB110-60531AF488
Alexa Fluor 647 Labeled NB110-60531AF647
Alexa Fluor 700 Labeled NB110-60531AF700

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Bioinformatics Tool for Apolipoprotein E/ApoE Antibody (NB110-60531AF405)

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Bioinformatics

Gene Symbol APOE
Entrez