RBFOX3/NeuN Antibody [FITC]

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Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications ICC/IF, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

RBFOX3/NeuN Antibody [FITC] Summary

Immunogen
A synthetic peptide made to an internal region of the human RBFOX3/NeuN protein (within residues 20-100). [Swiss-Prot A6NFN3]
Localization
Nucleus, Cytoplasm
Marker
Neuronal Marker
Predicted Species
Bovine (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RBFOX3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This RBFOX3/NeuN antibody is useful for ICC/IF and Immunohistochemistry-paraffin embedded sections. In ICC/IF, nuclear staining was observed in U2OS cells. In IHC-P, staining was observed in the nuclei of mouse brain cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human, rat and mouse. Immunogen has 95% identity to bovine.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for RBFOX3/NeuN Antibody [FITC]

  • FLJ56884
  • FLJ58356
  • Fox-1 homolog C
  • FOX3
  • FOX-3
  • hexaribonucleotide binding protein 3
  • HRNBP3
  • NeuN
  • neuronal nuclei
  • RBFOX3
  • RNA binding protein fox-1 homolog 3
  • RNA binding protein, fox-1 homolog (C. elegans) 3

Background

NeuN is a neuronal protein that was originally discovered by RJ Mullen et al. in 1994 through the use of specific monoclonal antibodies (PMID 1483388), and has since been widely used as a marker for many mature neuronal cell types. In 2009, Kim et al. (PMID 19713214) identified the protein responsible for NeuN's specificity, Fox-3. Fox-3 is a member of the Fox-1 family, which play a role in neural specific RNA splicing. Because of this, it is predominantly expressed in neuronal nuclei, but also can be seen in the cytoplasm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for RBFOX3/NeuN Antibody (NBP1-77686F) (0)

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Secondary Antibodies

 

Isotype Controls

Additional RBFOX3/NeuN Products

Bioinformatics Tool for RBFOX3/NeuN Antibody (NBP1-77686F)

Discover related pathways, diseases and genes to RBFOX3/NeuN Antibody (NBP1-77686F). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for RBFOX3/NeuN Antibody (NBP1-77686F)

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Pathways for RBFOX3/NeuN Antibody (NBP1-77686F)

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Research Areas for RBFOX3/NeuN Antibody (NBP1-77686F)

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Blogs on RBFOX3/NeuN.

RBFOX3: Binding RNA, like a FOX
NeuN is a RNA-binding protein that modulates alternative splicing and is localized both to the nucleus and cytoplasm. It is a member of the RNA-binding FOX (RBFOX) family of splicing regulators which includes RBFOX1 (Fox-1/A2BP1) and RBFOX2 (Fox-2/RBM...  Read full blog post.

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Bioinformatics

Gene Symbol RBFOX3