Nestin Antibody (10C2)

Images

 
Immunohistochemistry: Nestin Antibody (10C2) [NB300-266] - GFAP and Nestin immunoreactivity in retinal sections.Epifluorescent acquisition of p28 Reeler and WT retinas. (A-D) As compared to WT, both GFAP (red) and ...read more
Western Blot: Nestin Antibody (10C2) [NB300-266] - WB analysis of 5 ug lysate from (1) Rat's total brain tissue, and (2) human CNS progenitor cells using Nestin antibody (clone 10C2) at 1:1000 dilution. The antibody did ...read more
Immunocytochemistry/ Immunofluorescence: Nestin Antibody (10C2) [NB300-266] - Analysis of formalin fixed, 0.1% Triton X-100 permeabilized F9 cells (Mouse teratocarcinoma) using Nestin antibody (clone 10C2) at 1:50 ...read more
Immunohistochemistry-Paraffin: Nestin Antibody (10C2) [NB300-266] - Nestin was detected in immersion fixed paraffin-embedded sections of human kidney using anti-human mouse monoclonal antibody (Catalog # NB300-266) at ...read more
Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Nestin antibody (dark blue). Isotype control shown in orange. An antibody ...read more
Immunocytochemistry/ Immunofluorescence: Nestin Antibody (10C2) [NB300-266] - Visualization of Nestin in PC-3 cells using NB300-266. Courtesy of Dolnick Lab, Roswell Park Cancer Institute.
Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - An intracellular stain was performed on SH-SY5Y cells with Nestin [10C2] Antibody NB300-266C (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - An intracellular stain was performed on HeLa cells with Nestin Antibody (10C2)NB300-266PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more

Product Details

Summary
Reactivity Hu, Mu(-), Rb(-), Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
10C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2.4 mg/ml

Order Details

Nestin Antibody (10C2) Summary

Immunogen
A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681]
Localization
Cytoplasmic
Marker
Neural Stem Cell Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NES
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Frozen 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200
  • CyTOF-ready
Application Notes
This Nestin (10C2) antibody is useful for Western blot, Immunofluorescence/Immunocytochemistry, Flow Cytometry, Immunohistochemistry on formaldehyde fixed cultured cells and formalin fixed paraffin-embedded tissue sections. In Western blot a doublet band is seen at ~220-240 kDa. The investigator should determine the optimal working dilution for a specific application. This antibody is CyTOF ready.
Reviewed Applications
Read 2 Reviews rated 5
using
NB300-266 in the following applications:

Publications
Read Publications using
NB300-266 in the following applications:

  • 8 publications
  • IHC
    2 publications
  • 4 publications
  • WB
    3 publications

Reactivity Notes

It does not cross-react mouse, rat or rabbit.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
2.4 mg/ml
Purity
Protein G purified

Alternate Names for Nestin Antibody (10C2)

  • FLJ21841
  • NES
  • Nestin

Background

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nestin Antibody (NB300-266)(18)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(8)
IHC
(2)
IHC-P
(4)
WB
(3)
All Applications
Filter By Species
Human
(16)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB300-266 Applications Species
Sabelstrom H, Petri R, Shchors K et al. Driving Neuronal Differentiation through Reversal of an ERK1/2-miR-124-SOX9 Axis Abrogates Glioblastoma Aggressiveness Cell Rep Aug 20 2019 [PMID: 31433983] (ICC/IF, Human, Mouse) ICC/IF Human, Mouse
Adamska A, Pilacinski S, Zozulinska-Ziolkiewicz D et al. An increased skin microvessel density is associated with neurovascular complications in type 1 diabetes mellitus Diab Vasc Dis Res May 30 2019 [PMID: 31144511] (IHC-P, Human) IHC-P Human
Balzamino BO, Esposito G, Marino R et al. Changes in vitreal protein profile and retina mRNAs in Reeler mice: NGF, IL33 and Muller cell activation. PLoS One. 2019 Feb 27 [PMID: 30811468] (ICC/IF, Mouse) ICC/IF Mouse
Maxia Cristina, Murtas Daniela, Isola Michela et al. Immunophenotypic characterization of telocyte-like cells in pterygium. Molecular Vision 2018 Dec 29 [PMID: 30713424] (IHC-P, Human) IHC-P Human
Adamska A, Araszkiewicz A, Pilacinski S et al. Dermal microvessel density and maturity is closely associated with atherogenic dyslipidemia and accumulation of advanced glycation end products in adult patients with type 1 diabetes. Microvasc. Res. Oct 10 2018 [PMID: 30312628] (IHC-P, Human) IHC-P Human
Murtas D, Pilloni L, Diana A et al. Tyrosinase and nestin immunohistochemical expression in melanocytic nevi as a histopathologic pattern to trace melanocyte differentiation and nevogenesis. Histochem. Cell Biol. Sep 19 2018 [PMID: 30232588] (Human) Human
Iwata R, Maruyama M, Ito T et al. Establishment of a tumor sphere cell line from a metastatic brain neuroendocrine tumor. Med Mol Morphol May 17 2017 [PMID: 28516286]
Murtas D, Piras F, Minerba L et al. Activated Notch1 expression is associated with angiogenesis in cutaneous melanoma. Clin. Exp. Med. 2014 Jul 18 [PMID: 25034654] (IHC-P, Human)

Details:
Nestin antibody (clone 10C2) used as melanocyte stem cell marker in IHC-P staining of archival tissue block - 5um paraffin sections from primary human cutaneous melanomas and lymph node metastases - No antigen retrieval, primary diluted at 1:1000, detection via biotinylated anti-mouse secondary-alkaline phosphatase-streptavidin - liquid permanent red substrate-chromogen system (staining images not shown). Antibody also used for double immunofluorescence in tissue sections - 1:500 dilution /ON 4C incubation, detection with donkey Alexa Fluor 594 anti-mouse secondary (Figure 3).
IHC-P Human
Heroux MS, Chesnik MA, Halligan BD et al. Comprehensive Characterization of Glioblastoma Tumor Tissues for Biomarker Identification using Mass Spectrometry-based Label-free Quantitative Proteomics. Physiol. Genomics. 2014 May 06 [PMID: 24803679] (WB, Human) WB Human
Lai S, Piras F, Spiga S, Perra MT et al. Nestin and vimentin colocalization affects the subcellular location of glucocorticoid receptor in cutaneous melanoma Histopathology 2012 Aug 24 [PMID: 23072594] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Show All 18 Publications.

Reviews for Nestin Antibody (NB300-266) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB300-266:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
John H
WB Human 07/13/2017
View

Summary

ApplicationWestern Blot
Sample TestedPrimary glioblastoma cells - whole cell lysate,Primary glioblastoma cells
SpeciesHuman

Comments

Comments40 ug whole cell lysate
Blocking - 5% Milk TBST
Antibody - 1:2000 overnight at 4C
 
reviewed by:
Shama Mirza
WB Human 08/14/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsUsed this antibody for both Western blotting and Immunohistochemistry on frozen tissues. Worked well in both experiments.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nestin Antibody (NB300-266). (Showing 1 - 2 of 2 FAQs).

  1. We are interested in your item, NB300-266 and is it possible to provide me the % of predicted cross-reactivity with pig based on sequence homology?
    • The immunogen of NB300-266 is a fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681] and the BLAST results of this sequence may be seen at : Uniprot Site This sequence shows ~64% or less similarity with some un-characterized proteins in pig some of which might be NES. The alignment results of your mentioned sequence with the immunogen sequence of this product may be seen at: Uniprot Site There are several amino acids which are similar to immunogen of NB300-266, however, considering it being a monoclonal, I am not very hopeful that it will cross-react with the putative NES in pig. Accordingly, we will not guarantee it for Pig samples.
  2.  Why do you show the reactivity of mouse as “negative”? Could you provide me any further information for this description?
    • Upon review of the publication, it seems that the authors were testing nestin-GFP-transgenic mice. This may have affected the results versus endogenous detection, which we have performed in our lab. Per our testing, we have found that this clone usually does not detect the mouse protein in endogenous samples. That is why we list this species as negative on our datasheet. Additionally, the immunogen for this antibody only shares 45% homology to the mouse sequence.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-266AF350
Alexa Fluor 405 NB300-266AF405
Alexa Fluor 488 NB300-266AF488
Alexa Fluor 532 NB300-266AF532
Alexa Fluor 594 NB300-266AF594
Alexa Fluor 647 NB300-266AF647
Alexa Fluor 700 NB300-266AF700
Alexa Fluor 750 NB300-266AF750
Allophycocyanin NB300-266APC
Biotin NB300-266B
DyLight 350 NB300-266UV
DyLight 405 NB300-266V
DyLight 488 NB300-266G
DyLight 550 NB300-266R
DyLight 594 NB300-266DL594
DyLight 650 NB300-266C
DyLight 680 NB300-266FR
DyLight 755 NB300-266IR
FITC NB300-266F
HRP NB300-266H
Janelia Fluor 549 NB300-266JF549
Janelia Fluor 646 NB300-266JF646
PE NB300-266PE
PerCP NB300-266PCP

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Blogs on Nestin.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
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1
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John H
07/13/2017
Application: WB
Species: Human

 
Shama Mirza
08/14/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol NES