Nestin Antibody (10C2)


Western Blot: Nestin Antibody (10C2) [NB300-266] - WB analysis of 5 ug lysate from (1) Rat's total brain tissue, and (2) human CNS progenitor cells using Nestin antibody (clone 10C2) at 1:1000 dilution. The antibody did more
Immunocytochemistry/ Immunofluorescence: Nestin Antibody (10C2) [NB300-266] - Visualization of Nestin in PC-3 cells using NB300-266. Courtesy of Dolnick Lab, Roswell Park Cancer Institute.
Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Nestin antibody (dark blue). Isotype control shown in orange. An antibody more
Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - Staining of NTERA-2 cells using NB300-266 at a 1:50 dilution detected using DyLight-488 conjugated goat anti-mouse IgG secondary antibody.

Product Details

Reactivity Hu, Mu(-), Rb(-), Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
2.4 mg/ml

Nestin Antibody (10C2) Summary

A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681]
Neural Stem Cell Marker
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1% Sodium Azide
2.4 mg/ml
Protein G purified


Application Notes
This Nestin (10C2) antibody is useful for Western blot, Immunofluorescence/Immunocytochemistry, Flow Cytometry, Immunohistochemistry on formaldehyde fixed cultured cells and formalin fixed paraffin-embedded tissue sections. In Western blot a doublet band is seen at ~220-240 kDa. The investigator should determine the optimal working dilution for a specific application.
Reviewed Applications
Read 1 Review rated 5
NB300-266 in the following application:

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NB300-266 in the following applications:

Reactivity Notes

Human. It does not cross-react mouse, rat or rabbit.

Alternate Names for Nestin Antibody (10C2)

  • FLJ21841
  • NES
  • nestin


Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, GP, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
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Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Sh
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Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-Fr
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICFlow
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC-P, IP, PLA
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF
Species: Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB, IP
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF

Publications for Nestin Antibody (NB300-266)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB300-266 Applications Species
Murtas D, Piras F, Minerba L et al. Activated Notch1 expression is associated with angiogenesis in cutaneous melanoma. Clin. Exp. Med. 2014 Jul 18 [PMID:25034654] (IHC-P, Human) IHC-P Human
Heroux MS, Chesnik MA, Halligan BD et al. Comprehensive Characterization of Glioblastoma Tumor Tissues for Biomarker Identification using Mass Spectrometry-based Label-free Quantitative Proteomics. Physiol. Genomics. 2014 May 06 [PMID:24803679] (WB, Human) WB Human
Lai S, Piras F, Spiga S, Perra MT et al. Nestin and vimentin colocalization affects the subcellular location of glucocorticoid receptor in cutaneous melanoma Histopathology 2012 Aug 24 [PMID:23072594] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Kondo S, Wakisaka N, Muramatsu M, Zen Y, Endo K, Murono S, Sugimoto H, Yamaoka S, Pagano JS, Yoshizaki T. Epstein-Barr virus latent membrane protein 1 induces cancer stem/progenitor-like cells in nasopharyngeal epithelial cell lines. J Virol;85(21):11255-64. 2011 Nov. [PMID:21849440] (WB, Human) WB Human
Loudig O, Brandwein-Gensler M, Kim RS et al. Illumina whole-genome complementary DNA-mediated annealing, selection, extension and ligation platform: assessing its performance in formalin-fixed, paraffin-embedded samples and identifying invasion pattern-related genes in oral squamous cell carcinoma. Hum Pathol. 2011 Jun 16. [PMID:21683979] (IHC, Human) IHC Human
Porayette P, Gallego MJ, Kaltcheva MM, Bowen RL, Vadakkadath Meethal S, Atwood CS. Differential processing of amyloid-beta precursor protein directs human embryonic stem cell proliferation and differentiation into neuronal precursor cells. J Biol Chem;284(35):23806-17. 2009 Aug 28. [PMID:19542221] (WB, Human) WB Human
Ropolo M, Daga A, Griffero F et al. Comparative Analysis of DNA Repair in Stem Nonstem Glioma Cell Cultures. Mol Cancer Res;7(3):383-392. 2009 [PMID:19276180] (ICC/IF, Human) ICC/IF Human
Castriconi R, Daga A, Dondero A et al. NK Cells Recognize Kill Human Glioblastoma Cells with Stem Cell-Like Properties. J Immunol;182(6):3530-3539. 2009 [PMID:19265131] (ICC/IF, Human) ICC/IF Human
Gangemi RM, Griffero F, Marubbi D et al. SOX2 silencing in glioblastoma tumor-initiating cells causes stop of proliferation and loss of tumorigenicity. Stem Cells;27(1):40-8. 2009 Jan. [PMID:18948646] (ICC/IF, Human) ICC/IF Human
Wright LS, Li J, Caldwell MA, Wallace K, Johnson JA, Svendsen CN. Gene expression in human neural stem cells: effects of leukemia inhibitory factor. J Neurochem;86(1):179-95. 2003 Jul. [PMID:12807438] (ICC/IF, Human) ICC/IF Human
Show All 11 Publications.

Reviews for Nestin Antibody (NB300-266) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB300-266:
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Images Ratings Applications Species Date Details
reviewed by:
WB Human 08/14/2014


ApplicationWestern Blot
Special ApplicationsUsed this antibody for both Western blotting and Immunohistochemistry on frozen tissues. Worked well in both experiments.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nestin Antibody (NB300-266). (Showing 1 - 1 of 1 FAQs).

  1. We are interested in your item, NB300-266 and is it possible to provide me the % of predicted cross-reactivity with pig based on sequence homology?
    • The immunogen of NB300-266 is a fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681] and the BLAST results of this sequence may be seen at : Uniprot Site This sequence shows ~64% or less similarity with some un-characterized proteins in pig some of which might be NES. The alignment results of your mentioned sequence with the immunogen sequence of this product may be seen at: Uniprot Site There are several amino acids which are similar to immunogen of NB300-266, however, considering it being a monoclonal, I am not very hopeful that it will cross-react with the putative NES in pig. Accordingly, we will not guarantee it for Pig samples.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB300-266AF405
Alexa Fluor (R) 488 Labeled NB300-266AF488
Alexa Fluor (R) 647 Labeled NB300-266AF647
Alexa Fluor (R) 700 Labeled NB300-266AF700
Allophycocyanin Labeled NB300-266APC
Biotin Labeled NB300-266B
DyLight 350 Labeled NB300-266UV
DyLight 405 Labeled NB300-266V
DyLight 405LS Labeled NB300-266V3
DyLight 488 Labeled NB300-266G
DyLight 550 Labeled NB300-266R
DyLight 650 Labeled NB300-266C
DyLight 680 Labeled NB300-266FR
DyLight 755 Labeled NB300-266IR
FITC Labeled NB300-266F
HRP Labeled NB300-266H
PE Labeled NB300-266PE
PerCP Labeled NB300-266PCP

Additional Nestin Antibody (10C2) Products

Nestin NB300-266

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Blogs on Nestin.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol NES

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