This GAP43 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections and most histochemical applications, and Western blot where a band can be seen at 43-48 kDa. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24686445) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 4 using NBP1-41123 in the following applications:
Human, mouse, rat, primate, canine, feline and hamster.
Alternate Names for GAP-43 Antibody
Axonal membrane protein GAP-43
calmodulin-binding protein P-57
growth associated protein 43
Growth-associated protein 43
nerve growth-related peptide GAP43
Neural phosphoprotein B-50
neuron growth-associated protein 43
GAP43 (growth associated protein 43, also called B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1) is a major component of motile "growth cones" that form the tips of elongating axons as well as the synaptic plasma membranes. GAP43 is associated with nerve growth process and plays a key role in axonal as well as dendritic filopodia induction. It has been detected in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN, and binds calmodulin with superior affinity in the absence of Ca2+ than in its presence. Intracellular Ca2+ regulates PKC mediated phosphorylation of GAP43 and affects GAP43's ability to bind calmodulin. GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex. Aberrant expression of GAP43 has been seen in patients diagnosed with schizophrenia and Alzheimer's disease.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for GAP-43 Antibody (NBP1-41123). (Showing 1 - 2 of 2 FAQs).
Can you confirm the tested application of NBP1-41123 includes Immunohistochemistry on Formalin-Fixed Paraffin Embedded sections?
NBP1-41123 has only been validated in IHC on frozen sections at this time. However, NB300-143 has been validated in IHC on both frozen and paraffin-embedded tissues.
Could you tell us the sequence homology between the immunogen of this antibody and the GAP43 protein of canine?
The immunogen for NB300-143 shares 82% similarity with canine. NBP1-41123 may be a better choice. It is known to work in dog and in IHC. It has also been published with 10 times in IHC and some of these publications almost certainly used paraffin-embedded tissues.