GAP-43 Antibody


Western Blot: GAP-43 Antibody [NBP1-41123] - Analysis of GAP43 in human brain protein
Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NBP1-41123] - Staining of a mature retinal ganglion cell demonstrating axon outgrowth.

Product Details

Reactivity Hu, Mu, Rt, Ca, Fe, Ha, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

GAP-43 Antibody Summary

GAP43 from rat brain [UniProt# P07936]
Cell membrane; Peripheral membrane protein; Cytoplasmic side
Neuronal Marker
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
IgG purified


  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 1:400-1:1000
  • Immunohistochemistry-Frozen 1:400-1:1000
  • Immunohistochemistry-Paraffin
Application Notes
This GAP43 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections and most histochemical applications, and Western blot where a band can be seen at 43-48 kDa. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24686445) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
45 kDa
Reviewed Applications
Read 1 Review rated 4
NBP1-41123 in the following applications:

Read Publications using
NBP1-41123 in the following applications:

Reactivity Notes

Human, mouse, rat, primate, canine, feline and hamster.

Alternate Names for GAP-43 Antibody

  • Axonal membrane protein GAP-43
  • B-50
  • Basp2
  • calmodulin-binding protein P-57
  • GAP43
  • growth associated protein 43
  • Growth-associated protein 43
  • nerve growth-related peptide GAP43
  • Neural phosphoprotein B-50
  • Neuromodulin
  • neuron growth-associated protein 43
  • PP46
  • protein F1


GAP43 (growth associated protein 43, also called B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1) is a major component of motile "growth cones" that form the tips of elongating axons as well as the synaptic plasma membranes. GAP43 is associated with nerve growth process and plays a key role in axonal as well as dendritic filopodia induction. It has been detected in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN, and binds calmodulin with superior affinity in the absence of Ca2+ than in its presence. Intracellular Ca2+ regulates PKC mediated phosphorylation of GAP43 and affects GAP43's ability to bind calmodulin. GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex. Aberrant expression of GAP43 has been seen in patients diagnosed with schizophrenia and Alzheimer's disease.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GAP-43 Antibody (NBP1-41123)(23)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Hamster.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NBP1-41123 Applications Species
Puttagunta R, Tedeschi A, Soria MG et al. PCAF-dependent epigenetic changes promote axonal regeneration in the central nervous system. Nat Commun 2014 Apr 01 [PMID:24686445] (IHC-P, Mouse) IHC-P Mouse
James G, Foster SR, Key B, Beverdam A. The Expression Pattern of EVA1C, a Novel Slit Receptor, Is Consistent with an Axon Guidance Role in the Mouse Nervous System. PLoS One. 2013 Sep 9 [PMID:24040182] (IHC-Fr, ICC/IF, Mouse) IHC-Fr, ICC/IF Mouse
Fischer D, Petkova V, Thanos S, Benowitz LI. Switching mature retinal ganglion cells to a robust growth state in vivo: gene expression and synergy with RhoA inactivation. J Neurosci;24(40):8726-40. 2004 Oct 6. [PMID:15470139]
Fischer D, He Z, Benowitz LI. Counteracting the Nogo receptor enhances optic nerve regeneration if retinal ganglion cells are in an active growth state. J Neurosci;24(7):1646-51. 2004 Feb 18. [PMID:14973241]
Yin Y, Cui Q, Li Y, Irwin N, Fischer D, Harvey AR, Benowitz LI. Macrophage-derived factors stimulate optic nerve regeneration. J Neurosci;23(6):2284-93. 2003 Mar 15. [PMID:12657687] (IHC, ICC/IF, Rat) IHC, ICC/IF Rat
Chen P, Goldberg DE, Kolb B, Lanser M, Benowitz LI. Inosine induces axonal rewiring and improves behavioral outcome after stroke. Proc Natl Acad Sci U S A;99(13):9031-6. 2002 Jun 25. [PMID:12084941]
Leon S, Yin Y, Nguyen J, Irwin N, Benowitz LI. Lens injury stimulates axon regeneration in the mature rat optic nerve. J Neurosci;20(12):4615-26. 2000 Jun 15. [PMID:10844031] (IHC, ICC/IF) IHC, ICC/IF
Benowitz LI, Goldberg DE, Madsen JR, Soni D, Irwin N. Inosine stimulates extensive axon collateral growth in the rat corticospinal tract after injury. Proc Natl Acad Sci U S A;96(23):13486-90. 1999 Nov 9. [PMID:10557347] (IHC, Rat) IHC Rat
Perrone-Bizzozero NI, Sower AC, Bird ED, Benowitz LI, Ivins KJ, Neve RL. Levels of the growth-associated protein GAP-43 are selectively increased in association cortices in schizophrenia. Proc Natl Acad Sci U S A;93(24):14182-7. 1996 Nov 26. [PMID:8943081] (WB, Human) WB Human
Kinney HC, Rava LA, Benowitz LI. Anatomic distribution of the growth-associated protein GAP-43 in the developing human brainstem. J Neuropathol Exp Neurol;52(1):39-54. 1993 Jan. [PMID:8426187] (Human) Human
Show All 23 Publications.

Reviews for GAP-43 Antibody (NBP1-41123) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-41123:
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Images Ratings Applications Species Date Details
Western Blot GAP-43 NBP1-41123
reviewed by:
WB Rat 05/28/2015


ApplicationWestern Blot
Sample TestedRat olfactory epithelium tissue lysate


Blocking Details5% nonfat milk in TBS+0.1%Tween 18h at 4deg

Primary Anitbody

Dilution Ratio1/2000, 1hr, RT in TBS+0.1%Tween

Secondary Antibody

Secondary DescriptionDonkey Anti Sheep IgG, HRP
Secondary Manufacturer Cat#ab97125
Secondary Concentration1/5000 in 3.5% BSA


Detection NotesPierce supersignal west pico, 5min incubation, exposure 5s

Product General Protocols

View specific protocols for GAP-43 Antibody (NBP1-41123): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GAP-43 Antibody (NBP1-41123). (Showing 1 - 2 of 2 FAQs).

  1. Can you confirm the tested application of NBP1-41123 includes Immunohistochemistry on Formalin-Fixed Paraffin Embedded sections?
    • NBP1-41123 has only been validated in IHC on frozen sections at this time. However, NB300-143 has been validated in IHC on both frozen and paraffin-embedded tissues.
  2. Could you tell us the sequence homology between the immunogen of this antibody and the GAP43 protein of canine?
    • The immunogen for NB300-143 shares 82% similarity with canine. NBP1-41123 may be a better choice. It is known to work in dog and in IHC. It has also been published with 10 times in IHC and some of these publications almost certainly used paraffin-embedded tissues.

Secondary Antibodies


Isotype Controls

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Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

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Product PDFs


Gene Symbol GAP43

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