Western Blot: RBFOX3/NeuN Antibody [NBP1-77686] - Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody [NBP1-77686] - NeuN antibody was tested in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 ...read more
Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-77686] - JAK3 colocalized with neurons labeled with NeuN. Image collected and cropped by CiteAb from the following publication ...read more
Flow Cytometry: RBFOX3/NeuN Antibody [NBP1-77686] - An intracellular stain was performed on Hek293 cells with RBFOX3/NeuN Antibody NBP1-77686AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-77686] - IHC analysis of formalin fixed paraffin embedded tissue section from hippocampus of mouse brain with NeuN antibody at 1:200 dilution. The antibody ...read more
Immunohistochemistry: RBFOX3/NeuN Antibody [NBP1-77686] - Analysis of NeuN in mouse brain using DAB with hematoxylin counterstain.
Flow Cytometry: RBFOX3/NeuN Antibody [NBP1-77686] - An intracellular stain was performed on U2OS cells with RBFOX3/NeuN Antibody NBP1-77686AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
This NeuN antibody is useful for ICC and paraffin embedded sections. In ICC, nuclear staining was observed in U2OS cells. In IHC-P, staining was observed in the nuclei of mouse brain cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. IHC-Fr / IHC-FrFl application reported at 1:100 dilution with detailed protocol in PMID 22771622. Use in Western blot reported in scientific literature (PMID 26581336).
Human, rat and mouse. Immunogen has 95% identity to bovine. Bovine and porcine reactivity reported from a verified customer review.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for RBFOX3/NeuN Antibody
Fox-1 homolog C
hexaribonucleotide binding protein 3
neuronal nuclei antigen
RNA binding protein fox-1 homolog 3
RNA binding protein, fox-1 homolog (C. elegans) 3
RNA binding protein, fox-1 homolog 3
NeuN is a neuronal protein that was originally discovered by RJ Mullen et al. in 1994 through the use of specific monoclonal antibodies (PMID 1483388), and has since been widely used as a marker for many mature neuronal cell types. In 2009, Kim et al. (PMID 19713214) identified the protein responsible for NeuN's specificity, Fox-3. Fox-3 is a member of the Fox-1 family, which play a role in neural specific RNA splicing. Because of this, it is predominantly expressed in neuronal nuclei, but also can be seen in the cytoplasm.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Sipos E NEUROPATHOLOGICAL EXAMINATIONS IN THE MOUSE BRAININVESTIGATION OF A NEW PHARMACEUTICAL TARGET IN THE CUPRIZONE MODEL AND COMPARATIVE HISTOLOGICAL ANALYSES OF PRIMARY CILIA IN THE PHYSIOLOGICAL CENTRAL NERVOUS SYSTEM Thesis Jan 1 2020 (IHC-Fr, Mouse)
RBFOX3: Binding RNA, like a FOX NeuN is a RNA-binding protein that modulates alternative splicing and is localized both to the nucleus and cytoplasm. It is a member of the RNA-binding FOX (RBFOX) family of splicing regulators which includes RBFOX1 (Fox-1/A2BP1) and RBFOX2 (Fox-2/RBM... Read full blog post.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.