RBFOX3/NeuN Antibody

Images

 
Western Blot: RBFOX3/NeuN Antibody [NBP1-77686] - Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody [NBP1-77686] - NeuN antibody was tested in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 ...read more
Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-77686] - JAK3 colocalized with neurons labeled with NeuN. Image collected and cropped by CiteAb from the following publication ...read more
Flow Cytometry: RBFOX3/NeuN Antibody [NBP1-77686] - An intracellular stain was performed on Hek293 cells with RBFOX3/NeuN Antibody NBP1-77686AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody [NBP1-77686] - IHC analysis of formalin fixed paraffin embedded tissue section from hippocampus of mouse brain with NeuN antibody at 1:200 dilution. The antibody ...read more
Immunohistochemistry: RBFOX3/NeuN Antibody [NBP1-77686] - Analysis of NeuN in mouse brain using DAB with hematoxylin counterstain.
Flow Cytometry: RBFOX3/NeuN Antibody [NBP1-77686] - An intracellular stain was performed on U2OS cells with RBFOX3/NeuN Antibody NBP1-77686AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.08 mg/ml

RBFOX3/NeuN Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the human RBFOX3/NeuN protein (within residues 20-100). [Swiss-Prot A6NFN3]
Localization
Nucleus, Cytoplasm
Marker
Neuronal Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RBFOX3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunohistochemistry Free-Floating
Application Notes
This NeuN antibody is useful for ICC and paraffin embedded sections. In ICC, nuclear staining was observed in U2OS cells. In IHC-P, staining was observed in the nuclei of mouse brain cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. IHC-Fr / IHC-FrFl application reported at 1:100 dilution with detailed protocol in PMID 22771622. Use in Western blot reported in scientific literature (PMID 26581336).
Control
RBFOX3/NeuN Knockout HeLa Cell Lysate
Reviewed Applications
Read 7 Reviews rated 4.4
using
NBP1-77686 in the following applications:

Publications
Read Publications using
NBP1-77686 in the following applications:

Reactivity Notes

Human, rat and mouse. Immunogen has 95% identity to bovine. Bovine and porcine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.08 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RBFOX3/NeuN Antibody

  • anti-NeuN
  • FLJ56884
  • FLJ58356
  • Fox-1 homolog C
  • FOX3
  • FOX-3
  • hexaribonucleotide binding protein 3
  • HRNBP3
  • neuN antigen
  • NeuN ihc
  • NeuN immunohistochemistry
  • NeuN rabbit
  • NeuN staining
  • NeuN
  • neuronal nuclei antigen
  • neuronal nuclei
  • RBFOX3
  • RNA binding protein fox-1 homolog 3
  • RNA binding protein, fox-1 homolog (C. elegans) 3
  • RNA binding protein, fox-1 homolog 3

Background

NeuN is a neuronal protein that was originally discovered by RJ Mullen et al. in 1994 through the use of specific monoclonal antibodies (PMID 1483388), and has since been widely used as a marker for many mature neuronal cell types. In 2009, Kim et al. (PMID 19713214) identified the protein responsible for NeuN's specificity, Fox-3. Fox-3 is a member of the Fox-1 family, which play a role in neural specific RNA splicing. Because of this, it is predominantly expressed in neuronal nuclei, but also can be seen in the cytoplasm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RBFOX3/NeuN Antibody (NBP1-77686)(16)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Mouse .

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-FrFl, WB.


Filter By Application
ICC/IF
(5)
IHC
(4)
IHC-Fr
(5)
IHC-FrFl
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(8)
Rat
(5)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NBP1-77686 Applications Species
Stefani FR, Eberstal S, Vergani S et al. Low-dose irradiated mesenchymal stromal cells break tumor defensive properties in vivo Int. J. Cancer May 11 2018 [PMID: 29752716] (IHC, Mouse) IHC Mouse
Wilson CS, Dohare P, Orbeta S et al. Late Adolescence Mortality in Mice with Brain-Specific Deletion of the Volume-Regulated Anion Channel Subunit LRRC8A bioRxiv Jan 1 2020 (IHC-FrFl, Mouse) IHC-FrFl Mouse
Chen X, Jiang M, Li H et al. CX3CL1/CX3CR1 axis attenuates early brain injury via promoting the delivery of exosomal microRNA-124 from neuron to microglia after subarachnoid hemorrhage J Neuroinflammation Jul 14 2020 [PMID: 32664984] (ICC/IF, Human, Rat) ICC/IF Human, Rat
Kopper TJ, McFarlane KE, Bailey WM et al. Delayed Azithromycin Treatment Improves Recovery After Mouse Spinal Cord Injury Front Cell Neurosci Nov 6 2019 [PMID: 31780896] (IHC-Fr, Mouse) IHC-Fr Mouse
Taurine/Pilocarpine Interaction in the Malnourished Rat Brain: A Behavioral, Electrophysiological, and Immunohistochemical Analysis Front. Francisco E, Mendes-da-Silva R, Castro C et al. Front Neurosci [PMID: 31619952] (IHC-Fr, Rat) IHC-Fr Rat
Sipos E NEUROPATHOLOGICAL EXAMINATIONS IN THE MOUSE BRAININVESTIGATION OF A NEW PHARMACEUTICAL TARGET IN THE CUPRIZONE MODEL AND COMPARATIVE HISTOLOGICAL ANALYSES OF PRIMARY CILIA IN THE PHYSIOLOGICAL CENTRAL NERVOUS SYSTEM Thesis Jan 1 2020 (IHC-Fr, Mouse) IHC-Fr Mouse
Douglas AS, Shearer JA, Okolo A et al. The Relationship Between Cerebral Reperfusion And Regional Expression Of Matrix Metalloproteinase-9 In Rat Brain Following Focal Cerebral Ischemia Neuroscience Nov 18 2020 [PMID: 33220187] (ICC/IF, Rat)

Details:
Immunofluorescence staining brain of rats who underwent Middle Cerebral Artery Occlusion
ICC/IF Rat
Holmes A, Zhou N, Donahue DL et al. A deficiency of the GluN2C subunit of the N-methyl-D-aspartate receptor is neuroprotective in a mouse model of ischemic stroke Biochem. Biophys. Res. Commun. 2017 Oct 31 [PMID: 29101031] (Mouse) Mouse
DeMars KM, Pacheco SC, Yang C et al. Selective Inhibition of Janus Kinase 3 Has No Impact on Infarct Size or Neurobehavioral Outcomes in Permanent Ischemic Stroke in Mice. Front Neurol 2017 Aug 09 [PMID: 28790974] (ICC/IF, Mouse) ICC/IF Mouse
Zhang B, Bailey WM, McVicar AL, Gensel JC. Age increases reactive oxygen species production in macrophages and potentiates oxidative damage after spinal cord injury. Neurobiol Aging 2016 Nov [PMID: 27596335]
Show All 16 Publications.

Reviews for RBFOX3/NeuN Antibody (NBP1-77686) (7) 4.47

Average Rating: 4.4
(Based on 7 reviews)
We have 7 reviews tested in 7 species: Mouse, Rat, Bovine, Goat, HUman, Pig, human.

Reviews using NBP1-77686:
Filter by Applications
WB
(5)
Simple Western
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
Bovine
(1)
Goat
(1)
HUman
(1)
Pig
(1)
human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot RBFOX3/NeuN NBP1-77686
Enlarge
4
reviewed by:
Anonymous
WB Rat 07/28/2020
View

Summary

ApplicationWestern Blot
Sample Testedretina
SpeciesRat
LotC-1
Western Blot RBFOX3/NeuN NBP1-77686
Enlarge
4
reviewed by:
Anonymous
WB Mouse 07/28/2020
View

Summary

ApplicationWestern Blot
Sample Testedretina
SpeciesMouse
LotC-1
Simple Western RBFOX3/NeuN NBP1-77686
Enlarge
3
reviewed by:
Suman Chaudhary
Simple Western human 07/21/2020
View

Summary

ApplicationSimple Western
Sample TestedAdult eye and muscle tissue (labeled E and M
Specieshuman
LotC-1
Western Blot RBFOX3/NeuN NBP1-77686
Enlarge
5
reviewed by:
Anonymous
WB Bovine 07/21/2020
View

Summary

ApplicationWestern Blot
Sample Testedretina
SpeciesBovine
LotC-1
Western Blot RBFOX3/NeuN NBP1-77686
Enlarge
5
reviewed by:
Ajay Ashok
WB Pig 07/21/2020
View

Summary

ApplicationWestern Blot
Sample Testedretina
SpeciesPig
LotC-1
Western Blot RBFOX3/NeuN NBP1-77686
Enlarge
5
reviewed by:
Anonymous
WB Goat 07/21/2020
View

Summary

ApplicationWestern Blot
Sample Testedretina
SpeciesGoat
LotC-1
Immunocytochemistry RBFOX3/NeuN NBP1-77686
Enlarge
5
reviewed by:
Anonymous
ICC HUman 06/23/2020
View

Summary

ApplicationImmunocytochemistry
Sample Testedmixed primary neuronal culture
SpeciesHUman
LotC1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RBFOX3/NeuN Antibody (NBP1-77686) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-77686AF350
Alexa Fluor 405 NBP1-77686AF405
Alexa Fluor 488 NBP1-77686AF488
Alexa Fluor 532 NBP1-77686AF532
Alexa Fluor 594 NBP1-77686AF594
Alexa Fluor 647 NBP1-77686AF647
Alexa Fluor 700 NBP1-77686AF700
Alexa Fluor 750 NBP1-77686AF750
Biotin NBP1-77686B
DyLight 350 NBP1-77686UV
DyLight 405 NBP1-77686V
DyLight 488 NBP1-77686G
DyLight 550 NBP1-77686R
DyLight 594 NBP1-77686DL594
DyLight 650 NBP1-77686C
DyLight 680 NBP1-77686FR
DyLight 755 NBP1-77686IR
FITC NBP1-77686F
Janelia Fluor 549 NBP1-77686JF549
Janelia Fluor 646 NBP1-77686JF646

Additional RBFOX3/NeuN Products

Bioinformatics Tool for RBFOX3/NeuN Antibody (NBP1-77686)

Discover related pathways, diseases and genes to RBFOX3/NeuN Antibody (NBP1-77686). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for RBFOX3/NeuN Antibody (NBP1-77686)

Discover more about diseases related to RBFOX3/NeuN Antibody (NBP1-77686).
 

Pathways for RBFOX3/NeuN Antibody (NBP1-77686)

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Blogs on RBFOX3/NeuN.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

RBFOX3: Binding RNA, like a FOX
NeuN is a RNA-binding protein that modulates alternative splicing and is localized both to the nucleus and cytoplasm. It is a member of the RNA-binding FOX (RBFOX) family of splicing regulators which includes RBFOX1 (Fox-1/A2BP1) and RBFOX2 (Fox-2/RBM...  Read full blog post.

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Recent Reviews

4.4
5
4
4
2
3
1
2
0
1
0

Anonymous
07/28/2020
Application: WB
Species: Rat

Anonymous
07/28/2020
Application: WB
Species: Mouse

Suman Chaudhary
07/21/2020
Application: Simple Western
Species: human

Bioinformatics

Gene Symbol RBFOX3
Entrez
Uniprot