Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - analysis of GAPDH in HeLa and HEK 293 cells (25ug/lane) using anti-GAPDH antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: GAPDH Antibody (13H12) [NBP2-27103] - GAPDH antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of human rectal carcinoma tissue using GAPDH antibody (clone 13H12) at 5 ...read more
Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - WB detection of GAPDH protein in HeLa cells lysate using GAPDH antibody (clone 13H12) in (A) the absence and (B) the presence of immunizing peptide.
Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - WB detection of GAPDH protein in lysates of Mouse cell lines (A) NIH 3T3 (B) RAW 264.7 using GAPDH antibody (clone 13H12) at a concentration of 0.25 ug/ml.
Western Blot: GAPDH Antibody (13H12) [NBP2-27103] - WB detection of GAPDH protein in lysates of (A) HEK 293 cells and (B) Drosophila larvae using GAPDH antibody (clone 13H12) at a concentration of 0.25 ug/ml.
Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of normal human breast tissue using GAPDH antibody (clone 13H12) at 5 ...read more
Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of normal human stomach tissue using GAPDH antibody (clone 13H12) at 5 ...read more
Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of human colon tissue using GAPDH antibody (clone 13H12) at 5 ug/ml ...read more
Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [NBP2-27103] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using GAPDH ...read more
Simple Western: GAPDH/G3PDH Antibody (13H12) [NBP2-27103] - Simple Western lane view shows a specific band for GAPDH in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Hu, Mu, Rt, Dr, Pm, Sh, Po, Bv, Ca, Ft, GP, Re, SqSpecies Glossary
GAPDH is a widely used loading control for quantitative Western blotting. In IHC-P, the staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reacts with Human, Primates/Monkey, Mouse and Drosophila. Based upon 91% sequence similarity with immunogen, this antibody is predicted to react with Guinea Pig, Sheep, Squirrel, Porcine/Pig, Ferret, Canine/Dog/Cat, Bovine, Reptile / Rattlesnake and several other species. Immunogen shows 82% similarity to Xenopus and Zebrafish. Rat reactivity reported in scientific literature (PMID: 24796753). Sheep reactivity reported in scientific literature (PMID: 27618403).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Purified antibody in PBS containing 0.05% BSA.
0.05% Sodium Azide
Protein G purified
Alternate Names for GAPDH Antibody (13H12)
aging-associated gene 9 protein
GAPDPeptidyl-cysteine S-nitrosylase GAPDH
glyceraldehyde 3-phosphate dehydrogenase
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for GAPDH Antibody (NBP2-27103). (Showing 1 - 7 of 7 FAQs).
We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?
Well GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are lookin at. GAPDH antibody is a good choice for low to mid MW targets as GAPDH detects around 35 kDa.
I want to know why GAPDH is used as a loading control antibody in WB techniques.
Our GAPDH antibody makes a good loading control because GAPDH is a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?
This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody we already had but it didn't work. What can you recommend?
Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
What secondary antibody should I use for visualization of the GAPDH antibody?
This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
Do you offer a smaller size for the GAPDH antibodies?
We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.
I have GAPDH Antibody (NBP2-27103). According to the datasheet it is monoclonal. I want to do IF staining. Can it be stained with goat anti-rabbit IgG-FITC.
Our GAPDH antibody with catalogue number NBP2-27103 has been validated for immunofluorescence. Our own immunofluorescence testing data was generated in HeLa cells; detection was with a Dylight 488-conjugated anti-mouse secondary antibody. Since NBP2-27103 is a mouse antibody you will need to use an anti-mouse secondary antibody for detection; a goat anti-rabbit antibody will not be suitable. NBP2-27103 is of the isotype IgG1 kappa, and you can see our anti-mouse IgG1 secondary antibodies at the following link: anti-mouse IgG1 secondary antibodies You can use the search filters at the left hand side of our webpage to select your conjugate of choice.
GAPDH: More than a housekeeping gene GAPDH is a 146kD tetramer glycolytic pathway metabolic enzyme composed of four 30-40 kDa subunits. It is responsible for reversibly phosphorylating its substrate glyceraldehyde 3-phosphate within the glycolytic pathway. Apart from its role in gl... Read full blog post.
The GAPDH Antibody in Western blot Assays The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch... Read full blog post.
New Primers Available for ChIP Procedures Novus has recently released six new primer sets designed for use in real-time PCR DNA amplification of housekeeping, silent, or heterochromatin associated proteins. Specifically, the primers are for GAPDH, RPL30, MyoD1, AFM, alpha Satellite, and Satel... Read full blog post.
The GAPDH Antibody: A ChIP Off the Old Block GAPDH antibodies are commonly used as a loading control, due to GAPDH's widespread function as a house-keeping protein. As a leading scientific research antibody supplier, Novus Biologicals offer a wide range of GAPDH antibody reagents and support pro... Read full blog post.
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