PSD-95 Antibody (6G6-1C9)

Images

 
Western Blot: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of 25 ug of mouse brain (lane 1) and rat brain (lane 2) cell lysates.
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of Post Synaptic Density 95kD protein (PSD95, green) in cultured primary cortical neurons. Primary cortical neurons are isolated ...read more
Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in U87-MG cells compared to an isotype control (blue).
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) shows staining in HeLa cells. PSD95 staining (green), F-Actin staining with ...read more
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) shows staining in U251 glioma cells. PSD95 staining (green), F-Actin staining ...read more
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) shows staining in C6 glioma cells. PSD95 staining (green), F-Actin staining ...read more
Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in Neuro-2a cells compared to an isotype control (blue).
Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in SH-SY5Y cells compared to an isotype control (blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, In, PmSpecies Glossary
Applications WB, B/N, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
6G6-1C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
3.3 mg/ml

PSD-95 Antibody (6G6-1C9) Summary

Immunogen
Purified recombinant rat PSD-95.
Localization
Cytoplasmic
Marker
post-Synaptic Marker
Specificity
Detects Post Synaptic Density 95 kDa (PSD-95) from rat tissues.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
DLG4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Blocking/Neutralizing 50 - 200 molar excess
  • Flow Cytometry 2 ug / test (100ul)
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
WB: Detects an approx. 95 kDa protein and a slightly larger band in rat brain extracts. Flow usage was reported in scientific literature (PMID: 19187438). IHC usage was reported in scientific literature (PMID: 21289286). IHC-Fr usage was reported in scientific literature (PMID: 24875483).
Reviewed Applications
Read 1 Review rated 5
using
NB300-556 in the following applications:

Publications
Read Publications using
NB300-556 in the following applications:

Reactivity Notes

Invertebrate reactivity reported in scientific literature (PMID: 18182049). Primate reactivity reported in scientific literature (PMID: 20519524). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
3.3 mg/ml
Purity
Protein A purified

Alternate Names for PSD-95 Antibody (6G6-1C9)

  • discs, large homolog 4 (Drosophila)
  • disks large homolog 4
  • DLG4
  • FLJ97752
  • FLJ98574
  • Postsynaptic density protein 95
  • post-synaptic density protein 95
  • PSD95
  • PSD-95
  • PSD95SAP90SAP-90discs large homolog 4
  • SAP90
  • Synapse-associated protein 90
  • Tax interaction protein 15

Background

Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSD-95 Antibody (NB300-556)(11)

We have publications tested in 3 confirmed species: Mouse, Invertebrate, Mollusc.

We have publications tested in 3 applications: ICC/IF, IHC-Fr, WB.


Filter By Application
ICC/IF
(2)
IHC-Fr
(1)
WB
(3)
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Mouse
(1)
Invertebrate
(1)
Mollusc
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB300-556 Applications Species
Hussain S, Ringsevjen H, Egbenya DL et al. SNARE Protein Syntaxin-1 Colocalizes Closely with NMDA Receptor Subunit NR2B in Postsynaptic Spines in the Hippocampus. Front Mol Neurosci 2016 Feb 05 [PMID: 26903802]
Hussain S, Davanger S. Postsynaptic VAMP/Synaptobrevin Facilitates Differential Vesicle Trafficking of GluA1 and GluA2 AMPA Receptor Subunits. PLoS One 2015 [PMID: 26488171] (WB) WB
Naskar S, Wan H, Kemenes G. pT305-CaMkII stabilizes a learning-induced increase in AMPA receptors for ongoing memory consolidation after classical conditioning. Nat Commun. 2014 May 30 [PMID: 24875483] (IHC-Fr, Mollusc) IHC-Fr Mollusc
Giribaldi F, Milanese M, Bonifacino T, Anna Rossi PI, Di Prisco S, Pittaluga A, Tacchetti C, Puliti A, Usai C, Bonanno G. Group I metabotropic glutamate autoreceptors induce abnormal glutamate exocytosis in a mouse model of amyotrophic lateral sclerosis. Neuropharmacology. 2012 May 23. [PMID: 22634363] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Reissner KJ, Boyle HD, Ye X et al. Aplysia synapse associated protein (APSAP): identification, characterization, and selective interactions with Shaker-type potassium channels. J Neurochem. 2008 May [PMID: 18182049] (WB, ICC/IF, Invertebrate) WB, ICC/IF Invertebrate
Fogel AI, Akins MR, Krupp AJ et al. SynCAMs organize synapses through heterophilic adhesion. J Neurosci. 2007 Nov. [PMID: 18003830]
Furuyashiki T, Fujisawa K, Fujita A, Madaule P, Uchino S, Mishina M, Bito H, Narumiya S. Citron, a Rho-target, interacts with PSD-95/SAP-90 at glutamatergic synapses in the thalamus. J Neurosci19(1):109-18. 1999 Jan 1. [PMID: 9870943]
Zhang W et al. Citron binds to PSD-95 at glutamatergic synapses on inhibitory neurons in the hippocampus. J Neurosci 19:96-108. 1999 [PMID: 9870942]
Gomperts SN. Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family. Cell 84:659-62. 1996 [PMID: 8625403]
Patton BL et al. Autophosphorylation of type II CaM kinase in hippocampal neurons: localization of phospho- dephosphokinase with complementary phosphorylation site-specific antibodies. Mol Biol Cell 4:159-72. 1993 [PMID: 8443414]
Show All 11 Publications.

Review for PSD-95 Antibody (NB300-556) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: ICC.

Reviews using NB300-556:
Filter by Applications
ICC
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry PSD-95 NB300-556
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reviewed by:
Janosch Heller
ICC Rat 06/27/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedrat cortical culture (3 weeks old)
SpeciesRat

Comments

CommentsFixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT

Blocking Solution & Duration: 10% donkey serum in PBS with 0.1% triton, 30 minutes RT

Primary Antibody Diluent and Dilutions Tested: 1:650 in 5% donkey serum in PBS, over night 4°C

Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-mouse Alexa647, 1:1250 in PBS, 2 hours, RT

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for PSD-95 Antibody (NB300-556) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-556AF405
Alexa Fluor 488 Labeled NB300-556AF488
Alexa Fluor 647 Labeled NB300-556AF647
Alexa Fluor 700 Labeled NB300-556AF700
Allophycocyanin Labeled NB300-556APC
DyLight 350 Labeled NB300-556UV
DyLight 405 Labeled NB300-556V
DyLight 488 Labeled NB300-556G
DyLight 680 Labeled NB300-556FR
DyLight 755 Labeled NB300-556IR
PE Labeled NB300-556PE

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
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Janosch Heller
06/27/2017
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol DLG4
Entrez