PSD-95 Antibody (6G6-1C9)

Images

 
Western Blot: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of 25 ug of mouse brain (lane 1) and rat brain (lane 2) cell lysates.
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Control immunolocalization of plasma membrane AMPA receptor subunits (external epitopes) in hippocampal neuronal cultures. H) ...read more
Immunohistochemistry-Paraffin: PSD-95 Antibody (6G6-1C9) [NB300-556] - Confocal images of dissociated hippocampal cultures. Double labeling with anti-syntaxin-1 Double labeling with antibodies against syntaxin-1 (D) and ...read more
Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - An intracellular stain was performed on HeLa cells with PSD-95 Antibody (6G6-1C9) NB300-556AF488 (blue) and a matched isotype control (orange). Cells were fixed ...read more
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) shows staining in HeLa cells. PSD95 staining (green), F-Actin staining with ...read more
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) shows staining in U251 glioma cells. PSD95 staining (green), F-Actin staining ...read more
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 using PSD95 Monoclonal antibody (6G6-1C9) shows staining in C6 glioma cells. PSD95 staining (green), F-Actin staining ...read more
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of Post Synaptic Density 95kD protein (PSD95, green) in cultured primary cortical neurons. Primary cortical neurons are isolated ...read more
Immunocytochemistry/ Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Mixed 3 week old rat cortical cultures (21 DIV) were labelled with anti-PSD95 (1:650) and visualised with anti-mouse Alexa647 (1:2000). ...read more
Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in Neuro-2a cells compared to an isotype control (blue).
Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in SH-SY5Y cells compared to an isotype control (blue).
Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - Analysis of PSD95 in U87-MG cells compared to an isotype control (blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, In, PmSpecies Glossary
Applications WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N
Clone
6G6-1C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
3.3 mg/ml

PSD-95 Antibody (6G6-1C9) Summary

Immunogen
Purified recombinant rat PSD-95.
Localization
Cytoplasmic
Marker
post-Synaptic Marker
Specificity
Detects Post Synaptic Density 95 kDa (PSD-95) from rat tissues.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
DLG4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Flow Cytometry 2 ug / test (100ul)
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:2000
Application Notes
WB: Detects an approx. 95 kDa protein and a slightly larger band in rat brain extracts. Flow usage was reported in scientific literature (PMID: 19187438). IHC usage was reported in scientific literature (PMID: 21289286). IHC-Fr usage was reported in scientific literature (PMID: 24875483).
Reviewed Applications
Read 1 Review rated 5
using
NB300-556 in the following applications:

Publications
Read Publications using
NB300-556 in the following applications:

Reactivity Notes

Invertebrate reactivity reported in scientific literature (PMID: 18182049). Primate reactivity reported in scientific literature (PMID: 20519524). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
3.3 mg/ml
Purity
Protein A purified

Alternate Names for PSD-95 Antibody (6G6-1C9)

  • discs, large homolog 4 (Drosophila)
  • disks large homolog 4
  • DLG4
  • FLJ97752
  • FLJ98574
  • Postsynaptic density protein 95
  • post-synaptic density protein 95
  • PSD95
  • PSD-95
  • PSD95SAP90SAP-90discs large homolog 4
  • SAP90
  • Synapse-associated protein 90
  • Tax interaction protein 15

Background

Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-556
Species: Hu, Mu, Rt, In, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N

Publications for PSD-95 Antibody (NB300-556)(25)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Invertebrate, Mollusc.

We have publications tested in 4 applications: Flow, ICC/IF, IHC-Fr, WB.


Filter By Application
Flow
(1)
ICC/IF
(5)
IHC-Fr
(1)
WB
(9)
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Human
(1)
Mouse
(6)
Rat
(5)
Invertebrate
(1)
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(1)
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Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB300-556 Applications Species
Fogel AI, Akins MR, Krupp AJ et al. SynCAMs organize synapses through heterophilic adhesion. J Neurosci. 2007 Nov. [PMID: 18003830]
de Lima Marcolin M Influence of adolescent social instability stress on the intake of ethanol and sucrose in a rodent model Thesis (WB, Rat) WB Rat
Zarate N, Intihar T, Yu D Et al. Heat Shock Factor 1 Directly Regulates Postsynaptic Scaffolding PSD-95 in Aging and Huntington’s Disease and Influences Striatal Synaptic Density preprints Nov 4 2021 [PMID: 34884918] (WB, Mouse) WB Mouse
Ma KG, Hu HB, Zhou JS Et al. Neuronal Glypican4 promotes mossy fiber sprouting through the mTOR pathway after pilocarpine-induced status epilepticus in mice Experimental neurology Nov 5 2021 [PMID: 34748756] (WB, Mouse) WB Mouse
Bieler M, Hussain S, Daaland ESB et al. Changes in concentrations of NMDA receptor subunit GluN2B, Arc and syntaxin-1 in dorsal hippocampus Schaffer collateral synapses in a rat learned helplessness model of depression The Journal of comparative neurology Apr 12 2021 [PMID: 33843051]
Diaz A, Merino P, McCann P et al. Urokinase-type plasminogen activator promotes N-cadherin-mediated synaptic recovery in the ischemic brain Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Mar 24 2021 [PMID: 33757316]
Sulistomo HW, Nemoto T, Kage Y et al. Fhod3 Controls the Dendritic Spine Morphology of Specific Subpopulations of Pyramidal Neurons in the Mouse Cerebral Cortex Cerebral cortex (New York, N.Y. : 1991) Nov 30 2020 [PMID: 33251537]
Ritzel RM, Li Y, He J Sustained neuronal and microglial alterations are associated with diverse neurobehavioral dysfunction long after experimental brain injury Neurobiol Dis. [PMID: 31843705] (Flow, Mouse) Flow Mouse
Gomperts SN. Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family. Cell 84:659-62. 1996 [PMID: 8625403]
Patton BL et al. Autophosphorylation of type II CaM kinase in hippocampal neurons: localization of phospho- dephosphokinase with complementary phosphorylation site-specific antibodies. Mol Biol Cell 4:159-72. 1993 [PMID: 8443414]
Show All 25 Publications.

Review for PSD-95 Antibody (NB300-556) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB300-556:
Filter by Applications
ICC
(1)
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Filter by Species
Rat
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry PSD-95 NB300-556
Enlarge
5
reviewed by:
Janosch Heller
ICC Rat 06/27/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedrat cortical culture (3 weeks old)
SpeciesRat

Comments

CommentsFixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT

Blocking Solution & Duration: 10% donkey serum in PBS with 0.1% triton, 30 minutes RT

Primary Antibody Diluent and Dilutions Tested: 1:650 in 5% donkey serum in PBS, over night 4°C

Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-mouse Alexa647, 1:1250 in PBS, 2 hours, RT

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for PSD-95 Antibody (NB300-556) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-556AF350
Alexa Fluor 405 NB300-556AF405
Alexa Fluor 488 NB300-556AF488
Alexa Fluor 532 NB300-556AF532
Alexa Fluor 594 NB300-556AF594
Alexa Fluor 647 NB300-556AF647
Alexa Fluor 700 NB300-556AF700
Alexa Fluor 750 NB300-556AF750
Allophycocyanin NB300-556APC
DyLight 350 NB300-556UV
DyLight 405 NB300-556V
DyLight 488 NB300-556G
DyLight 594 NB300-556DL594
DyLight 650 NB300-556C
DyLight 680 NB300-556FR
DyLight 755 NB300-556IR
Janelia Fluor 549 NB300-556JF549
Janelia Fluor 646 NB300-556JF646
PE NB300-556PE

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Blogs on PSD-95.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Janosch Heller
06/27/2017
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol DLG4
Entrez