NK1R Antibody

Images

 
Western Blot: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Analysis of Neurokinin 1 Receptor in 1: human brain lysate, 2: rat brain lysate and 3: monkey brain lysate.
Immunocytochemistry/ Immunofluorescence: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Immunostain of NK1R in rat enteric glial cells. Image from verified customer review.
Immunohistochemistry: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Staining of human brain, basal nucleus of Meynert, neurons.
Western Blot: NK1R Antibody [NB300-119] - Total protein from human, mouse and rat brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was ...read more
Western Blot: NK1R Antibody [NB300-119] - TACR1 and its downstream targets SRC and p-SRC are expressed in neuroblastoma cell lines. Endogenous TACR1 (NK1R), SRC and p-SRC expression in whole-cell extracts of ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, GpSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

NK1R Antibody Summary

Immunogen
A peptide derived from the N-terminus region between amino acids 1-50 of human Neurokinin 1 Receptor. [UniProt# P25103]
Localization
Cell Membrane
Predicted Species
Guinea Pig (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TACR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 10 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in IHC-F reported in scientific literature (PMID: 17604979).
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NK1R Overexpression Lysate
Control Peptide
NK1R Peptide (NB300-119PEP)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB300-119 in the following applications:

Publications
Read Publications using
NB300-119 in the following applications:

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NK1R Antibody

  • NK1R
  • NK-1R
  • NK1Rneurokinin 1 receptor
  • NKIR
  • SPR
  • SPRsubstance-P receptor
  • TAC1R
  • TAC1Rtachykinin 1 receptor (substance P receptor, neurokinin 1 receptor)
  • tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor)
  • tachykinin receptor 1NK-1 receptor
  • TACR1

Background

The tachykinins belong to an evolutionary conserved family of peptide neurotransmitters that share the c-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an established role in neurotransmission. The mammalian tachykinins include substance P, neurokinin A (NKA) and neurokinin B (NKB) which exert their effects by binding to specific receptors. Tachykinin peptides are important in the mediation of many physiological and pathological processes including inflammation, pain, migraine headache and allergy induced asthma. Three tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 which have preferential affinities for SP, NKA and NKB respectively. All three receptors share a high degree of sequence homology, have seven transmembrane spanning domains and similar signal transduction mechanisms (e.g. G-protein coupled activation of phospholipase C).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-187
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC
MAB4077
Species: Hu
Applications: WB, IHC
NB600-41532
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-15304
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB300-102
Species: Mu, Rt, Gp, Sh, Hu(-)
Applications: WB (-), ICC/IF, IHC, IHC-Fr, IHC-FrFl
NB300-201
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-44501
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IF
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-19788
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
NB300-201
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-61118
Species: Hu, Mu
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
NBP2-67717
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-91258
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB300-119
Species: Hu, Mu, Rt, Gp
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for NK1R Antibody (NB300-119)(36)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
FLOW
(1)
ICC/IF
(2)
IHC
(13)
IHC-Fr
(1)
IHC-P
(1)
WB
(10)
All Applications
Filter By Species
Human
(19)
Mouse
(13)
Rat
(2)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using NB300-119 Applications Species
Xu X, Cai X, Liu X, Guo SW Possible involvement of neuropeptide and neurotransmitter receptors in Adenomyosis Reproductive biology and endocrinology : RB&E Feb 19 2021 [PMID: 33602248] (IHC, Human) IHC Human
Morelli Ae, Sumpter Tl, Rojas-Canales Dm et Al. Neurokinin-1 Receptor Signaling Is Required for Efficient Ca2+ Flux in T-Cell-Receptor-Activated T Cells Cell Rep Mar 10 2020 [PMID: 32160549] (WB, Mouse) WB Mouse
Zhou Y, Wang M, Tong Y et al. miR-206 Promotes Cancer Progression by Targeting Full-Length Neurokinin-1 Receptor in Breast Cancer Technol. Cancer Res. Treat. Jan 1 2019 [PMID: 31506061] (IHC, WB, Human, Mouse) IHC, WB Human, Mouse
Li Q, Wu X, Yang Y et al. Tachykinin NK1 receptor antagonist L-733,060 and substance P deletion exert neuroprotection through inhibiting oxidative stress and cell death after traumatic brain injury in mice Int. J. Biochem. Cell Biol. Dec 26 2018 [PMID: 30593954] (WB, Mouse) WB Mouse
Bubak AN, Como CN, Blackmon AM et al. Varicella Zoster Virus Induces Nuclear Translocation of the Neurokinin-1 Receptor, Promoting Lamellipodia Formation and Viral Spread in Spinal Astrocytes J. Infect. Dis. May 19 2018 [PMID: 29788447] (WB) WB
Wang Dong, Tawfik Vivianne L, Corder Gregory et al. Functional Divergence of Delta and Mu Opioid Receptor Organization in CNS Pain Circuits. Genome Medicine 2018 Apr 4 [PMID: 29576387] (IHC, Mouse) IHC Mouse
Werner DF. Elucidating the Role of Alpha1-containing GABA(A) Receptors in Ethanol Action PhD Thesis University of Pittsburgh (ISBN 0549455426, 9780549455424). 2007 (WB, Mouse) WB Mouse
Zhang Z, Zheng W, Xie H et al. Up-regulated expression of substance P in CD8(+) T cells and NK1R on monocytes of atopic dermatitis. J Transl Med May 1 2017 [PMID: 28460633] (Mouse) Mouse
Henssen AG, Odersky A, Szymansky A et al. Targeting tachykinin receptors in neuroblastoma. Oncotarget. Nov 18 2016 [PMID: 27888795] (WB, Human) WB Human
Zhou Y, Zuo D, Wang M et al. Effect of truncated neurokinin-1 receptor expression changes on the interaction between human breast cancer and bone marrow-derived mesenchymal stem cells. Genes Cells. 2014 Sep 01 [PMID: 25130457] (WB, Human) WB Human
Show All 36 Publications.

Reviews for NK1R Antibody (NB300-119) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NB300-119:
Filter by Applications
WB
(1)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Megan Janssen-Schroeder
WB Mouse 12/27/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse
CommentsNo complaints at all with this antibody. Thank you!

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000

Comments

CommentsNo complaints at all with this antibody. Thank you!
Immunohistochemistry-Paraffin NK1R NB300-119
Enlarge
5
reviewed by:
satya gunnam
IHC-P Rat 04/26/2010
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedRat enteric glial cells
SpeciesRat
CommentsUsed primary ata a conc of 1:100 and Tritc conjugated secondary at 1:200 dilutions

Blocking

Blocking DetailsBlocking Buffer: 10% Normal Donkey serum and 5% BSA, Blocking Time: 2 hours, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: TRITC CONJUGATED DONKEY ANTI RABBIT, Secondary Ab Dilution: 1:200
Secondary Manufacturer Cat#711-025-152 Jackson Immuno Research

Details

Detection NotesDetection Method: FLUORESCENCE, Positive Control: HEK transfected with NK-1R, Negative Control: Hek cells
Fixation DetailsSample Preparation: 4% PFA fixed

Comments

CommentsUsed primary ata a conc of 1:100 and Tritc conjugated secondary at 1:200 dilutions

Product General Protocols

View specific protocols for NK1R Antibody (NB300-119): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NK1R Antibody (NB300-119). (Showing 1 - 4 of 4 FAQs).

  1. What amino acid residues are recognized by the anti- Neurokinin 1 Receptor antibody NB300-119?
    • The exact immunogen sequence is deemed proprietary by the lab, and we have not epitope mapped this particular antibody. Therefore we do not know exactly where NB300-119 binds to its target. However, the immunogen was derived from the N-terminus of the human Neurokinin 1 Receptor protein. If there is a specific region that you are interested in targeting, or excluding, please send your desired sequence information to our Technical Support team at technical@novusbio.com, for comparison to the immunogen sequence used to synthesize the antibody.
  2. Our customer would like to know the MW that this antibody can detect in western blot.
    • The MW of this protein is 46KDa.
  3. One of our customers is willing to purchase this antibody, before placing the order she would like to know if it can be used for formalin-fix, paraffin-embedded sections in immunohistochemistry?
    • Yes, this product has been validated for use in IHC paraffin embedded sections.
  4. I have been trying this antibody for Western blot incortex and spinal cord from rat without results. Is there any reference where they have done WB with this antibody?
    • At this time we do not have any references found where this has been described in WB, and of the references we have they are all for use in staining applications. We do have a product review however from a customer who observed positive results in WB as well as our own QC testing data that show positive staining in brain samples. This link (Product Specific Protocol) is also the recommended protocol for this one that may be helpful to you. If you have any difficulties with this one we would be happy to have the lab take a look at your results and compare them to our QC tests.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-119AF350
Alexa Fluor 405 NB300-119AF405
Alexa Fluor 488 NB300-119AF488
Alexa Fluor 532 NB300-119AF532
Alexa Fluor 594 NB300-119AF594
Alexa Fluor 647 NB300-119AF647
Alexa Fluor 700 NB300-119AF700
Alexa Fluor 750 NB300-119AF750
Allophycocyanin/Cy7 NB300-119APCCY7
Biotin NB300-119B
DyLight 350 NB300-119UV
DyLight 405 NB300-119V
DyLight 488 NB300-119G
DyLight 550 NB300-119R
DyLight 594 NB300-119DL594
DyLight 650 NB300-119C
DyLight 680 NB300-119FR
DyLight 755 NB300-119IR
FITC NB300-119F
HRP NB300-119H
Janelia Fluor 549 NB300-119JF549
Janelia Fluor 646 NB300-119JF646
PE NB300-119PE
PE/Atto594 NB300-119PEATT594
PE/Cy5.5 NB300-119PECY55
PE/Cy7 NB300-119PECY7

Additional NK1R Products

Bioinformatics Tool for NK1R Antibody (NB300-119)

Discover related pathways, diseases and genes to NK1R Antibody (NB300-119). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NK1R Antibody (NB300-119)

Discover more about diseases related to NK1R Antibody (NB300-119).
 

Pathways for NK1R Antibody (NB300-119)

View related products by pathway.

PTMs for NK1R Antibody (NB300-119)

Learn more about PTMs related to NK1R Antibody (NB300-119).
 

Research Areas for NK1R Antibody (NB300-119)

Find related products by research area.

Blogs on NK1R

There are no specific blogs for NK1R, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Megan Janssen-Schroeder
12/27/2011
Application: WB
Species: Mouse

satya gunnam
04/26/2010
Application: IHC-P
Species: Rat

Bioinformatics

Gene Symbol TACR1