NK1R Antibody - BSA Free

Images

 
Western Blot: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Analysis of Neurokinin 1 Receptor in 1: human brain lysate, 2: rat brain lysate and 3: monkey brain lysate.
Immunocytochemistry/ Immunofluorescence: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Immunostain of NK1R in rat enteric glial cells. Image from verified customer review.
Immunohistochemistry: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Staining of human brain, basal nucleus of Meynert, neurons.
Western Blot: NK1R Antibody [NB300-119] - Total protein from human, mouse and rat brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was ...read more
Western Blot: NK1R Antibody [NB300-119] - TACR1 and its downstream targets SRC and p-SRC are expressed in neuroblastoma cell lines. Endogenous TACR1 (NK1R), SRC and p-SRC expression in whole-cell extracts of ...read more
Immunocytochemistry/ Immunofluorescence: NK1R Antibody [NB300-119] - Overnight incubation at 4C with NK1R antibody diluted 1:100 in PBS/BSA 1%. Red: Phalloidin, Blue: DAPI, Green: NK1R antibody. Image from verified ...read more
TACR1 and its downstream targets SRC and p-SRC are expressed in neuroblastoma cell lines. (A) Endogenous TACR1, SRC and p-SRC expression in whole-cell extracts of neuroblastoma cell lines was visualized by western ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, GpSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

NK1R Antibody - BSA Free Summary

Immunogen
A peptide derived from the N-terminus region between amino acids 1-50 of human Neurokinin 1 Receptor. [UniProt# P25103]
Localization
Cell Membrane
Predicted Species
Guinea Pig (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TACR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 17604979)
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NK1R Overexpression Lysate
Control Peptide
NK1R Peptide (NB300-119PEP)
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB300-119 in the following applications:

Publications
Read Publications using
NB300-119 in the following applications:

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NK1R Antibody - BSA Free

  • NK1R
  • NK-1R
  • NK1Rneurokinin 1 receptor
  • NKIR
  • SPR
  • SPRsubstance-P receptor
  • TAC1R
  • TAC1Rtachykinin 1 receptor (substance P receptor, neurokinin 1 receptor)
  • tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor)
  • tachykinin receptor 1NK-1 receptor
  • TACR1

Background

The tachykinins belong to an evolutionary conserved family of peptide neurotransmitters that share the c-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an established role in neurotransmission. The mammalian tachykinins include substance P, neurokinin A (NKA) and neurokinin B (NKB) which exert their effects by binding to specific receptors. Tachykinin peptides are important in the mediation of many physiological and pathological processes including inflammation, pain, migraine headache and allergy induced asthma. Three tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 which have preferential affinities for SP, NKA and NKB respectively. All three receptors share a high degree of sequence homology, have seven transmembrane spanning domains and similar signal transduction mechanisms (e.g. G-protein coupled activation of phospholipase C).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NK1R Antibody (NB300-119)(42)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 7 applications: FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
FLOW
(2)
ICC/IF
(2)
IF/IHC
(13)
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
WB
(13)
All Applications
Filter By Species
Human
(21)
Mouse
(14)
Rat
(2)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 42. Show All 42 Publications.
Publications using NB300-119 Applications Species
Zheng Y, Sang M, Liu F et al. Aprepitant inhibits the progression of esophageal squamous cancer by blocking the truncated neurokinin?1 receptor Oncology reports 2023-07-01 [PMID: 37203393] (WB, Human) WB Human
Restaino AC, Walz A, Vermeer SJ et al. Functional neuronal circuits promote disease progression in cancer Science advances 2023-05-10 [PMID: 37163587] (WB, IHC, Human)

Details:
IHC Dilution: 1:500; WB Dilution: 1:500
WB, IHC Human
Zhu E, Liu Y, Zhong M et al. Targeting NK-1R attenuates renal fibrosis via modulating inflammatory responses and cell fate in chronic kidney disease Frontiers in Immunology 2023-03-24 [PMID: 37033943] (IHC) IHC
Brzozowska M, Romaniewicz M, Calka J, Jana B Effects of Substance P and Neurokinin A on the Contractile Activity of Inflamed Porcine Uterus International Journal of Molecular Sciences 2022-10-29 [PMID: 36361972] (WB, Porcine) WB Porcine
Guan M, Ying S, Wang Y. Increased expression of transient receptor potential channels and neurogenic factors associates with cough severity in a guinea pig model BMC Pulmonary Medicine 2021-12-01 [PMID: 34078339]
Xu X, Cai X, Liu X, Guo SW Possible involvement of neuropeptide and neurotransmitter receptors in Adenomyosis Reproductive biology and endocrinology : RB&E 2021-02-19 [PMID: 33602248] (IF/IHC, Human) IF/IHC Human
Morelli Ae, Sumpter Tl, Rojas-Canales Dm et Al. Neurokinin-1 Receptor Signaling Is Required for Efficient Ca2+ Flux in T-Cell-Receptor-Activated T Cells Cell Rep 2020-03-10 [PMID: 32160549] (WB, Mouse) WB Mouse
Talbot S, Doyle B, et al. Vagal sensory neurons drive mucous cell metaplasia. J Allergy Clin Immunol 2020-06-01 [PMID: 31954778] (FLOW, Mouse) FLOW Mouse
Zhou Y, Wang M, Tong Y et al. miR-206 Promotes Cancer Progression by Targeting Full-Length Neurokinin-1 Receptor in Breast Cancer Technol. Cancer Res. Treat. 2019-01-01 [PMID: 31506061] (WB, IF/IHC, Human, Mouse) WB, IF/IHC Human, Mouse
Li Q, Wu X, Yang Y et al. Tachykinin NK1 receptor antagonist L-733,060 and substance P deletion exert neuroprotection through inhibiting oxidative stress and cell death after traumatic brain injury in mice Int. J. Biochem. Cell Biol. 2018-12-26 [PMID: 30593954] (WB, Mouse) WB Mouse
Show All 42 Publications.

Reviews for NK1R Antibody (NB300-119) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB300-119:
Filter by Applications
ICC
(1)
WB
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry NK1R NB300-119
Enlarge
4
reviewed by:
Patricia Carulla Martí
ICC Human 06/28/2023
View

Summary

ApplicationImmunocytochemistry
Sample TestedCultured Human Keratinocytes
SpeciesHuman
LotM-10
  4
reviewed by:
Megan Janssen-Schroeder
WB Mouse 12/27/2011
View

Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse
  5
reviewed by:
satya gunnam
IHC-P Rat 04/26/2010
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedRat enteric glial cells
SpeciesRat

Product General Protocols

View specific protocols for NK1R Antibody (NB300-119): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NK1R Antibody (NB300-119). (Showing 1 - 4 of 4 FAQs).

  1. What amino acid residues are recognized by the anti- Neurokinin 1 Receptor antibody NB300-119?
    • The exact immunogen sequence is deemed proprietary by the lab, and we have not epitope mapped this particular antibody. Therefore we do not know exactly where NB300-119 binds to its target. However, the immunogen was derived from the N-terminus of the human Neurokinin 1 Receptor protein. If there is a specific region that you are interested in targeting, or excluding, please send your desired sequence information to our Technical Support team at technical@novusbio.com, for comparison to the immunogen sequence used to synthesize the antibody.
  2. Our customer would like to know the MW that this antibody can detect in western blot.
    • The MW of this protein is 46KDa.
  3. One of our customers is willing to purchase this antibody, before placing the order she would like to know if it can be used for formalin-fix, paraffin-embedded sections in immunohistochemistry?
    • Yes, this product has been validated for use in IHC paraffin embedded sections.
  4. I have been trying this antibody for Western blot incortex and spinal cord from rat without results. Is there any reference where they have done WB with this antibody?
    • At this time we do not have any references found where this has been described in WB, and of the references we have they are all for use in staining applications. We do have a product review however from a customer who observed positive results in WB as well as our own QC testing data that show positive staining in brain samples. This link (Product Specific Protocol) is also the recommended protocol for this one that may be helpful to you. If you have any difficulties with this one we would be happy to have the lab take a look at your results and compare them to our QC tests.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for NK1R Antibody (NB300-119)

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Blogs on NK1R

There are no specific blogs for NK1R, but you can read our latest blog posts.
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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Patricia Carulla Martí
06/28/2023
Application: ICC
Species: Human

 
Megan Janssen-Schroeder
12/27/2011
Application: WB
Species: Mouse

 
satya gunnam
04/26/2010
Application: IHC-P
Species: Rat

Bioinformatics

Gene Symbol TACR1