NK1R Antibody - BSA Free

Western Blot: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Analysis of Neurokinin 1 Receptor in 1: human brain lysate, 2: rat brain lysate and 3: monkey brain lysate.

Immunocytochemistry/Immunofluorescence: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Immunostain of NK1R in rat enteric glial cells. Image from verified customer review.

Immunohistochemistry: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Staining of human brain, basal nucleus of Meynert, neurons.

Western Blot: NK1R Antibody [NB300-119] - Total protein from human, mouse and rat brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-NK1R in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Western Blot: NK1R Antibody [NB300-119] - TACR1 and its downstream targets SRC and p-SRC are expressed in neuroblastoma cell lines. Endogenous TACR1 (NK1R), SRC and p-SRC expression in whole-cell extracts of neuroblastoma cell lines was visualized by western blotting. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.13440), licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt, Gp
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NK1R Antibody - BSA Free Summary

• Immunogen: A peptide derived from the N-terminus region between amino acids 1-50 of human Neurokinin 1 Receptor. [UniProt# P25103]
• Localization: Cell Membrane
• Predicted Species: Guinea Pig (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TACR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in IHC-F reported in scientific literature (PMID: 17604979).
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NK1R Overexpression Lysate

• Control Peptide:

  NK1R Peptide (NB300-119PEP)

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NK1R Antibody - BSA Free

• NK1R
• NK-1R
• NK1Rneurokinin 1 receptor
• NKIR
• SPR
• SPRsubstance-P receptor
• TAC1R
• TAC1Rtachykinin 1 receptor (substance P receptor, neurokinin 1 receptor)
• tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor)
• tachykinin receptor 1NK-1 receptor
• TACR1

Background

The tachykinins belong to an evolutionary conserved family of peptide neurotransmitters that share the c-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an established role in neurotransmission. The mammalian tachykinins include substance P, neurokinin A (NKA) and neurokinin B (NKB) which exert their effects by binding to specific receptors. Tachykinin peptides are important in the mediation of many physiological and pathological processes including inflammation, pain, migraine headache and allergy induced asthma. Three tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 which have preferential affinities for SP, NKA and NKB respectively. All three receptors share a high degree of sequence homology, have seven transmembrane spanning domains and similar signal transduction mechanisms (e.g. G-protein coupled activation of phospholipase C).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NK1R Antibody (NB300-119)(38)

Publications using NB300-119

• Brzozowska M, Romaniewicz M, Calka J, Jana B Effects of Substance P and Neurokinin A on the Contractile Activity of Inflamed Porcine Uterus International Journal of Molecular Sciences 2022-10-29 [PMID: 36361972] (WB, Porcine)
• Xu X, Cai X, Liu X, Guo SW Possible involvement of neuropeptide and neurotransmitter receptors in Adenomyosis Reproductive biology and endocrinology : RB&E 2021-02-19 [PMID: 33602248] (IHC, Human)
• Morelli Ae, Sumpter Tl, Rojas-Canales Dm et Al. Neurokinin-1 Receptor Signaling Is Required for Efficient Ca2+ Flux in T-Cell-Receptor-Activated T Cells Cell Rep 2020-03-10 [PMID: 32160549] (WB, Mouse)
• Talbot S, Doyle B, et al. Vagal sensory neurons drive mucous cell metaplasia. J Allergy Clin Immunol 2020-06-01 [PMID: 31954778] (FLOW, Mouse)
• Zhou Y, Wang M, Tong Y et al. miR-206 Promotes Cancer Progression by Targeting Full-Length Neurokinin-1 Receptor in Breast Cancer Technol. Cancer Res. Treat. 2019-01-01 [PMID: 31506061] (IHC, WB, Human, Mouse)
• Li Q, Wu X, Yang Y et al. Tachykinin NK1 receptor antagonist L-733,060 and substance P deletion exert neuroprotection through inhibiting oxidative stress and cell death after traumatic brain injury in mice Int. J. Biochem. Cell Biol. 2018-12-26 [PMID: 30593954] (WB, Mouse)
• Wang Dong, Tawfik Vivianne L, Corder Gregory et al. Functional Divergence of Delta and Mu Opioid Receptor Organization in CNS Pain Circuits. Genome Medicine 2018-04-04 [PMID: 29576387] (IHC, Mouse)
• Zhang Z, Zheng W, Xie H et al. Up-regulated expression of substance P in CD8(+) T cells and NK1R on monocytes of atopic dermatitis. J Transl Med 2017-05-01 [PMID: 28460633] (Mouse)
• Henssen AG, Odersky A, Szymansky A et al. Targeting tachykinin receptors in neuroblastoma. Oncotarget. 2016-11-18 [PMID: 27888795] (WB, Human)
• Zhou Y, Zuo D, Wang M et al. Effect of truncated neurokinin-1 receptor expression changes on the interaction between human breast cancer and bone marrow-derived mesenchymal stem cells. Genes Cells. 2014-09-01 [PMID: 25130457] (WB, Human)
• Fujisawa D, Kashiwakura JI, Kita H et al. Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria. J. Allergy Clin. Immunol. 2014-06-19 [PMID: 24954276] (FLOW, Human)
  
  Details:
  NK-1R on the cell surface of Human skin/lung-derived cultured Mast cells (MCs) was analyzed by using flow cytometric analysis. MCs fixed in 4% paraformaldehyde ( 20 minutes at 4°C) were incubated for 30 minutes at 4°C with anti–NK-1R polyclonal antibody and detected via biotinylated goat anti-rabbit IgG - phycoerythrin-conjugated streptavidin using the FACSCalibur /analyzed with FlowJo software. The MCs were found to be negative for the signal whereas the positive control Jurkat cells expressed NK-1R ( Figure 1D)
• Zhou Y, Zhao L, Xiong T et al. Roles of full-length and truncated neurokinin-1 receptors on tumor progression and distant metastasis in human breast cancer. Breast Cancer Res Treat 2013-07-01 [PMID: 23807418] (IHC-P, Human)
• Werner DF. Elucidating the Role of Alpha1-containing GABA(A) Receptors in Ethanol Action PhD Thesis University of Pittsburgh (ISBN 0549455426, 9780549455424). 2007-01-01 (WB, Mouse)
• Bubak AN, Como CN, Blackmon AM et al. Varicella Zoster Virus Induces Nuclear Translocation of the Neurokinin-1 Receptor, Promoting Lamellipodia Formation and Viral Spread in Spinal Astrocytes J. Infect. Dis. 2018-05-19 [PMID: 29788447] (WB)
• Schwartz L, Spitsin SV, Meshki J et al. Substance P enhances HIV-1 infection in human fetal brain cell cultures expressing full-length neurokinin-1 receptor. J Neurovirol 2013-06-14 [PMID: 23765222] (ICC/IF, Mouse)
• Humes, D J, Simpson, J, Smith, J et al. Visceral hypersensitivity in symptomatic diverticular disease and the role of neuropeptides and low grade inflammation Neurogastroenterology & Motility 2012-01-01 [PMID: 22276853] (IHC, Human)
• Altawil R, Lystrom J, El-Nour H. Kinetics of neuronal contribution during the development of a contact allergic reaction. Arch Dermatol Res 2012-05-01 [PMID: 22198823] (IHC, Human)
  
  Details:
  Using the DyLight 650 conjugated version of NB300-119, catalog number NB300-119C.
• Janssen MJ, Yasuda RP, Vicini S. GABA(A) Receptor Beta3 Subunit Expression Regulates Tonic Current in Developing Striatopallidal Medium Spiny Neurons. Front Cell Neurosci 2011-07-28 [PMID: 21847370] (WB, Mouse)
• Nayate A et al. Cardiac damage after lesions of the nucleus tractus solitarii. Am J Physiol Regul Integr Comp Physiol;296(2):R272-9. 2009-02-01 [PMID: 19020288] (IHC, Rat)
• Massouh M, Wallman MJ, Pourcher E, Parent A. The fate of the large striatal interneurons expressing calretinin in Huntington's disease. Neurosci Res 2008-12-01 [PMID: 18801393] (Human)
• Schmidtko A et al. cGMP produced by NO-sensitive guanylyl cyclase essentially contributes to inflammatory and neuropathic pain by using targets different from cGMP-dependent protein kinase I. J Neurosci;28(34):8568-76. 2008-08-20 [PMID: 18716216] (IHC, Mouse)
• Andersson G, Danielson P, Alfredson H, Forsgren S. Presence of substance P and the neurokinin-1 receptor in tenocytes of the human Achilles tendon. Regul Pept 2008-10-09 [PMID: 18394729] (Human)
• Grimsholm O, Rantapaa-Dahlqvist S, Dalen T, Forsgren S. Observations favouring the occurrence of local production and marked effects of bombesin/gastrin-releasing peptide in the synovial tissue of the human knee joint--comparisons with substance P and the NK-1 receptor. Neuropeptides 2008-04-01 [PMID: 18289674] (Human)
• Ferguson C, Hardy SL, Werner DF et al. New insight into the role of the beta3 subunit of the GABAA-R in development, behavior, body weight regulation, and anesthesia revealed by conditional gene knockout. BMC Neurosci. 2007-10-10 [PMID: 17927825] (WB, Mouse)
• Andersson G, Danielson P, Alfredson H et al. Nerve-related characteristics of ventral paratendinous tissue in chronic Achilles tendinosis. Knee Surg Sports Traumatol Arthrosc. 2007-10-01 [PMID: 17604979] (IHC-Fr, Human)
• Hazlett, LD et al. Spantide I decreases type I cytokines, enhances IL-10, and reduces corneal perforation in susceptible mice after Pseudomonas aeruginosa infection. Invest Ophthalmol Vis Sci;48(2):797-807. 2007-02-01 [PMID: 17251480] (IHC, Mouse)
• Levesque M, Wallman MJ, Parent R et al. Neurokinin-1 and neurokinin-3 receptors in primate substantia nigra. Neurosci Res 2007-03-01 [PMID: 17134780]
• Fong, AY, Potts, JT. Neurokinin-1 receptor activation in Botzinger complex evokes bradypnoea. J Physiol;575(Pt 3):869-85. 2006-09-15 [PMID: 16825299] (IHC, Rat)
• Levesque M, Parent R, Parent A. Cellular and subcellular localization of neurokinin-1 and neurokinin-3 receptors in primate globus pallidus. Eur J Neurosci 2006-05-01 [PMID: 16817879]
• Borghese CM, Werner DF, Topf N et al. An isoflurane- and alcohol-insensitive mutant GABA(A) receptor alpha(1) subunit with near-normal apparent affinity for GABA: characterization in heterologous systems and production of knockin mice. J Pharmacol Exp Ther. 2006-10-01 [PMID: 16807363] (WB, Mouse)
• Lai JP, Ho WZ, Kilpatrick LE et al. Full-length and truncated neurokinin-1 receptor expression and function during monocyte/macrophage differentiation. Proc Natl Acad Sci U S A 2006-05-16 [PMID: 16675550] (ICC/IF, Human)
• Persson-Sjogren S, Lejon K, Holmberg D, Forsgren S. Expression of the NK-1 receptor on islet cells and invading immune cells in the non-obese diabetic mouse. J Autoimmun 2005-06-01 [PMID: 15869864] (Mouse)
• Jonsson M, Norrgard O, Forsgren S. Substance P and the neurokinin-1 receptor in relation to eosinophilia in ulcerative colitis. Peptides 2005-05-01 [PMID: 15808910] (Human)
• Forsgren S, Danielson P, Alfredson H. Vascular NK-1 receptor occurrence in normal and chronic painful Achilles and patellar tendons: studies on chemically unfixed as well as fixed specimens. Regul Pept 2005-03-30 [PMID: 15664664] (Human)
• Benarroch, E. Depletion of ventromedullary NK-1 receptor-immunoreactive neurons in multiple system atrophy. Brain;126(Pt 10):2183-90. 2003-10-01 [PMID: 12902309] (IHC, Human)
• Shrikhe, S et al. NK-1 receptor gene expression is related to pain in chronic pancreatitis. Pain;91(3):209-17. 2001-04-01 [PMID: 11275376] (IHC, Human)
• Tooney, PA et al. Increased tachykinin NK(1) receptor immunoreactivity in the prefrontal cortex in schizophrenia. Biol Psychiatry;49(6):523-7. 2001-03-15 [PMID: 11257237] (IHC, Human)
• Tooney, Paul A et al. Localisation of tachykinin NK1 and NK3 receptors in the human prefrontal and visual cortex. Neurosci Lett;283(3):185-8. 2000-04-14 [PMID: 10754218] (IHC, Human)

Reviews for NK1R Antibody (NB300-119) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Rat.

reviewed by: Verified Customer WB Mouse 12/27/2011

Summary

• Application: Western Blot
• Sample Tested: Mouse
• Species: Mouse

reviewed by: Verified Customer IHC-P Rat 04/26/2010

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Rat enteric glial cells
• Species: Rat

FAQs for NK1R Antibody (NB300-119). (Showing 1 - 4 of 4 FAQs).

1. What amino acid residues are recognized by the anti- Neurokinin 1 Receptor antibody NB300-119?
   • The exact immunogen sequence is deemed proprietary by the lab, and we have not epitope mapped this particular antibody. Therefore we do not know exactly where NB300-119 binds to its target. However, the immunogen was derived from the N-terminus of the human Neurokinin 1 Receptor protein. If there is a specific region that you are interested in targeting, or excluding, please send your desired sequence information to our Technical Support team at technical@novusbio.com, for comparison to the immunogen sequence used to synthesize the antibody.
2. Our customer would like to know the MW that this antibody can detect in western blot.
   • The MW of this protein is 46KDa.
3. One of our customers is willing to purchase this antibody, before placing the order she would like to know if it can be used for formalin-fix, paraffin-embedded sections in immunohistochemistry?
   • Yes, this product has been validated for use in IHC paraffin embedded sections.
4. I have been trying this antibody for Western blot incortex and spinal cord from rat without results. Is there any reference where they have done WB with this antibody?
   • At this time we do not have any references found where this has been described in WB, and of the references we have they are all for use in staining applications. We do have a product review however from a customer who observed positive results in WB as well as our own QC testing data that show positive staining in brain samples. This link (Product Specific Protocol) is also the recommended protocol for this one that may be helpful to you. If you have any difficulties with this one we would be happy to have the lab take a look at your results and compare them to our QC tests.

Bioinformatics

• Gene Symbol: TACR1