NK1R Antibody

Images

 
Western Blot: NK1R Antibody [NB300-119] - Total protein from human, mouse and rat brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was ...read more
Immunocytochemistry/ Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-119] - Immunostain of NK1R in rat enteric glial cells. Image courtesy of anonymous customer review.
Immunohistochemistry: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Staining of human brain, basal nucleus of Meynert, neurons.
Western Blot: Neurokinin 1 Receptor Antibody [NB300-119] - Analysis of Neurokinin 1 Receptor in 1. human brain lysate, 2. rat brain lysate and 3. monkey brain lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, GPSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NK1R Antibody Summary

Immunogen
A peptide derived from the N-terminus region between amino acids 1-50 of human Neurokinin 1 Receptor [UniProt# P25103]
Localization
Cell Membrane
Predicted Species
Guinea Pig (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TACR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
Application Notes
This Neurokinin 1 Receptor Antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Western blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NK1R Lysate (NBL1-16672)
Control Peptide
NK1R Peptide (NB300-119PEP)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB300-119 in the following applications:

Publications
Read Publications using
NB300-119 in the following applications:

Reactivity Notes

Immunogen has 94% sequence identity with guinea pig. Mouse reactivity is from a customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NK1R Antibody

  • NK1R
  • NK-1R
  • NK1Rneurokinin 1 receptor
  • NKIR
  • SPR
  • SPRsubstance-P receptor
  • TAC1R
  • TAC1Rtachykinin 1 receptor (substance P receptor, neurokinin 1 receptor)
  • tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor)
  • tachykinin receptor 1NK-1 receptor
  • TACR1

Background

The tachykinins belong to an evolutionary conserved family of peptide neurotransmitters that share the c-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an established role in neurotransmission. The mammalian tachykinins include substance P, neurokinin A (NKA) and neurokinin B (NKB) which exert their effects by binding to specific receptors. Tachykinin peptides are important in the mediation of many physiological and pathological processes including inflammation, pain, migraine headache and allergy induced asthma. Three tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 which have preferential affinities for SP, NKA and NKB respectively. All three receptors share a high degree of sequence homology, have seven transmembrane spanning domains and similar signal transduction mechanisms (e.g. G-protein coupled activation of phospholipase C).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NK1R Antibody (NB300-119)(31)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


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FLOW
(1)
ICC/IF
(2)
IHC
(10)
IHC-Fr
(1)
IHC-P
(1)
WB
(7)
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Human
(17)
Mouse
(9)
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(2)
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Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using NB300-119 Applications Species
Bubak AN, Como CN, Blackmon AM et al. Varicella Zoster Virus Induces Nuclear Translocation of the Neurokinin-1 Receptor, Promoting Lamellipodia Formation and Viral Spread in Spinal Astrocytes J. Infect. Dis. May 19 2018 [PMID: 29788447] (WB) WB
Zhang Z, Zheng W, Xie H et al. Up-regulated expression of substance P in CD8(+) T cells and NK1R on monocytes of atopic dermatitis. J Transl Med May 1 2017 [PMID: 28460633] (Mouse) Mouse
Henssen AG, Odersky A, Szymansky A et al. Targeting tachykinin receptors in neuroblastoma. Oncotarget. Nov 18 2016 [PMID: 27888795] (WB, Human) WB Human
Fujisawa D, Kashiwakura JI, Kita H et al. Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria. J. Allergy Clin. Immunol. 2014 Jun 19 [PMID: 24954276] (FLOW, Human)

Details:
NK-1R on the cell surface of Human skin/lung-derived cultured Mast cells (MCs) was analyzed by using flow cytometric analysis. MCs fixed in 4% paraformaldehyde ( 20 minutes at 4°C) were incubated for 30 minutes at 4°C with anti–NK-1R polyclonal antibody and detected via biotinylated goat anti-rabbit IgG - phycoerythrin-conjugated streptavidin using the FACSCalibur /analyzed with FlowJo software. The MCs were found to be negative for the signal whereas the positive control Jurkat cells expressed NK-1R ( Figure 1D)
FLOW Human
Werner DF. Elucidating the Role of Alpha1-containing GABA(A) Receptors in Ethanol Action PhD Thesis University of Pittsburgh (ISBN 0549455426, 9780549455424). 2007 (WB, Mouse) WB Mouse
Zhou Y, Zuo D, Wang M et al. Effect of truncated neurokinin-1 receptor expression changes on the interaction between human breast cancer and bone marrow-derived mesenchymal stem cells. Genes Cells. 2014 Sep 01 [PMID: 25130457] (WB, Human) WB Human
Zhou Y, Zhao L, Xiong T et al. Roles of full-length and truncated neurokinin-1 receptors on tumor progression and distant metastasis in human breast cancer. Breast Cancer Res Treat 2013 Jul [PMID: 23807418] (IHC-P, Human) IHC-P Human
Schwartz L, Spitsin SV, Meshki J et al. Substance P enhances HIV-1 infection in human fetal brain cell cultures expressing full-length neurokinin-1 receptor. J Neurovirol 2013 Jun 14 [PMID: 23765222] (ICC/IF, Mouse) ICC/IF Mouse
Humes, D J, Simpson, J, Smith, J et al. Visceral hypersensitivity in symptomatic diverticular disease and the role of neuropeptides and low grade inflammation Neurogastroenterology & Motility 2012 [PMID: 22276853] (IHC, Human) IHC Human
Altawil R, Lystrom J, El-Nour H. Kinetics of neuronal contribution during the development of a contact allergic reaction. Arch Dermatol Res 2012 May [PMID: 22198823] (IHC, Human)

Details:
Using the DyLight 650 conjugated version of NB300-119, catalog number NB300-119C.
IHC Human
Show All 31 Publications.

Reviews for NK1R Antibody (NB300-119) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NB300-119:
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WB
(1)
IHC-P
(1)
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Mouse
(1)
Rat
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Megan Janssen-Schroeder
WB Mouse 12/27/2011
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Summary

ApplicationWestern Blot
Sample TestedMouse
SpeciesMouse
CommentsNo complaints at all with this antibody. Thank you!

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000

Comments

CommentsNo complaints at all with this antibody. Thank you!
Immunohistochemistry-Paraffin NK1R NB300-119
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reviewed by:
satya gunnam
IHC-P Rat 04/26/2010
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedRat enteric glial cells
SpeciesRat
CommentsUsed primary ata a conc of 1:100 and Tritc conjugated secondary at 1:200 dilutions

Blocking

Blocking DetailsBlocking Buffer: 10% Normal Donkey serum and 5% BSA, Blocking Time: 2 hours, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: TRITC CONJUGATED DONKEY ANTI RABBIT, Secondary Ab Dilution: 1:200
Secondary Manufacturer Cat#711-025-152 Jackson Immuno Research

Details

Detection NotesDetection Method: FLUORESCENCE, Positive Control: HEK transfected with NK-1R, Negative Control: Hek cells
Fixation DetailsSample Preparation: 4% PFA fixed

Comments

CommentsUsed primary ata a conc of 1:100 and Tritc conjugated secondary at 1:200 dilutions

Product General Protocols

View specific protocols for NK1R Antibody (NB300-119): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NK1R Antibody (NB300-119). (Showing 1 - 4 of 4 FAQs).

  1. What amino acid residues are recognized by the anti- Neurokinin 1 Receptor antibody NB300-119?
    • The exact immunogen sequence is deemed proprietary by the lab, and we have not epitope mapped this particular antibody. Therefore we do not know exactly where NB300-119 binds to its target. However, the immunogen was derived from the N-terminus of the human Neurokinin 1 Receptor protein. If there is a specific region that you are interested in targeting, or excluding, please send your desired sequence information to our Technical Support team at technical@novusbio.com, for comparison to the immunogen sequence used to synthesize the antibody.
  2. Our customer would like to know the MW that this antibody can detect in western blot.
    • The MW of this protein is 46KDa.
  3. One of our customers is willing to purchase this antibody, before placing the order she would like to know if it can be used for formalin-fix, paraffin-embedded sections in immunohistochemistry?
    • Yes, this product has been validated for use in IHC paraffin embedded sections.
  4. I have been trying this antibody for Western blot incortex and spinal cord from rat without results. Is there any reference where they have done WB with this antibody?
    • At this time we do not have any references found where this has been described in WB, and of the references we have they are all for use in staining applications. We do have a product review however from a customer who observed positive results in WB as well as our own QC testing data that show positive staining in brain samples. This link (Product Specific Protocol) is also the recommended protocol for this one that may be helpful to you. If you have any difficulties with this one we would be happy to have the lab take a look at your results and compare them to our QC tests.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-119AF405
Alexa Fluor 488 Labeled NB300-119AF488
Alexa Fluor 647 Labeled NB300-119AF647
Alexa Fluor 700 Labeled NB300-119AF700
Biotin Labeled NB300-119B
DyLight 405 Labeled NB300-119V
DyLight 488 Labeled NB300-119G
DyLight 550 Labeled NB300-119R
DyLight 650 Labeled NB300-119C
FITC Labeled NB300-119F
HRP Labeled NB300-119H
PE Labeled NB300-119PE

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Megan Janssen-Schroeder
12/27/2011
Application: WB
Species: Mouse

satya gunnam
04/26/2010
Application: IHC-P
Species: Rat

Bioinformatics

Gene Symbol TACR1
Entrez