Apolipoprotein E/ApoE Antibody (WUE-4) [PerCP]

• Reactivity: Hu, Mu, Rt(-)
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: WUE-4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PerCP

Apolipoprotein E/ApoE Antibody (WUE-4) [PerCP] Summary

• Immunogen: Purified human ApoE [UniProt# P02649]
• Epitope: Residues 140-160 of purified human ApoE (LDLR receptor binding site).
• Localization: Secreted
• Specificity: This antibody detects ApoE2, ApoE3 and ApoE4 (PMID 2466929).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: APOE
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Reacts with human better than mouse. It does not appear to react with rat brain tissue.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for Apolipoprotein E/ApoE Antibody (WUE-4) [PerCP]

• APOE
• Apo-E
• Apolipoprotein E
• apolipoprotein E3
• late onset)
• MGC1571

Background

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein E/ApoE Antibody (NB110-60531PCP)(1)

Publication using NB110-60531PCP

• Brachyahu MK, Krisztina N, Sayam G et al. Reduced circulating CD63+ extracellular vesicle levels associate with atherosclerosis in hypercholesterolaemic mice and humans Cardiovasc Diabetol. 2024-10-17 [PMID: 39420340] (Flow Cytomery, Human, Mouse)

Bioinformatics

• Gene Symbol: APOE