Apolipoprotein E/ApoE Antibody (WUE-4) [DyLight 550]

• Reactivity: Hu, Mu, Rt(-)
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: WUE-4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 550

Apolipoprotein E/ApoE Antibody (WUE-4) [DyLight 550] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Purified human ApoE [UniProt# P02649]
• Epitope: Residues 140-160 of purified human ApoE (LDLR receptor binding site).
• Localization: Secreted
• Specificity: This antibody detects ApoE2, ApoE3 and ApoE4 (PMID 2466929).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: APOE
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB110-60531. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Reacts with human better than mouse. It does not appear to react with rat brain tissue.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Apolipoprotein E/ApoE Antibody (WUE-4) [DyLight 550]

• APOE
• Apo-E
• Apolipoprotein E
• apolipoprotein E3
• late onset)
• MGC1571

Background

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes. Apolipoprotein E is a type of lipoprotein. Apolipoprotein E is associated with several cardiovascular disorders. There are three isoforms (slightly different forms) of the ApoE lipoprotein. These three isoforms are known as ApoE2, E3 and E4. ApoE2 seems to be one of several genetic factors that plays a part in increased risk of heart attacks and strokes and ApoE4 is a risk factor for coronary artery disease. As well, ApoE4 has been been suggested to play a role in type 2 Alzheimer disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein E/ApoE Antibody (NB110-60531R)(1)

Publication using NB110-60531R

• Huynh TV, Liao F, Francis CM et al. Age-Dependent Effects of apoE Reduction Using Antisense Oligonucleotides in a Model of beta-amyloidosis. Neuron. 2017-12-06 [PMID: 29216448]

Bioinformatics

• Gene Symbol: APOE