CD133 Antibody [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

Order Details

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CD133 Antibody [DyLight 650] Summary

Immunogen
Synthetic peptide corresponding to a C-terminal region of CD133 (within amino acids 750-865).
Marker
Stem Cell Marker
Specificity
CD133 - Hematopoietic Stem Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PROM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
ELISA: 1/1000. WB: 1/1,000 - 1:2,000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). IHC: Citrate buffer antigen retreival required. ICC/IF: Fix with 3% paraformaldehyde for 20 min at RT. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Reacts with human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD133 Antibody [DyLight 650]

  • AC133 Stargardt disease 4 (autosomal dominant)
  • AC133
  • CD133 retinal 2
  • CD133
  • PROM1
  • prominin (mouse)-like 1
  • Prominin 1
  • PROML1

Background

Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD133 Antibody (NB120-16518C) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 488 Labeled NB120-16518G
DyLight 550 Labeled NB120-16518R

Additional CD133 Products

CD133 NB120-16518C

Bioinformatics Tool for CD133 Antibody (NB120-16518C)

Discover related pathways, diseases and genes to CD133 Antibody (NB120-16518C). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CD133 Antibody (NB120-16518C)

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Pathways for CD133 Antibody (NB120-16518C)

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PTMs for CD133 Antibody (NB120-16518C)

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Research Areas for CD133 Antibody (NB120-16518C)

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Blogs on CD133.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

CD133
Also known as PROM1 and AC133, this gene is located on chromosome 4p15 and encodes CD133, a 120kDa pentaspan transmembrane glycoprotein (5-TM) and presents multiple spliced variants. Prominin-1 (CD133) was the first protein identified as "Prominin"; o...  Read full blog post.

CD Antibodies Uncover Markers for Rare Breast Cancer
We at Novus Biologicals have added several new products to our CD antibody database. The CD, or Cluster of Differential proteins are a family of type I transmembrane glycoproteins widely expressed in immune cell populations. These include B cells, thy...  Read full blog post.

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Bioinformatics

Gene Symbol PROM1
Entrez