SCP3/SYCP3 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: SCP3 Antibody [NB300-231] - SCP3 labeled in mouse pachytene preparation (red), using NB 300-231. CDK2 staining, near teleomeres, is also present (green).
Immunohistochemistry: SCP3 Antibody [NB300-231] - Punctate staining of murine SCP3 in mouse ovary using NB300-231.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

SCP3/SYCP3 Antibody Summary

Immunogen
A synthetic peptide made to the C-terminal region of the mouse SCP3 protein. [UniProt# P70281]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYCP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1:100-1:750
  • Immunohistochemistry 1:750
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:750
Application Notes
This SCP3 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections.
Control Peptide
SCP3/SYCP3 Peptide (NB300-231PEP)
Publications
Read Publications using
NB300-231 in the following applications:

Reactivity Notes

Mouse.Human reactivity reported in scientific literature (PMID: 21079786)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SCP3/SYCP3 Antibody

  • COR1
  • MGC71888
  • SCP3 chromosome 3 open reading frame 8
  • SCP3 COR1
  • SCP3
  • SCP-3
  • SYCP3
  • synaptonemal complex protein 3

Background

SCP3 (Synaptonemal complex protein 3) is a DNA-binding nuclear protein that belongs to XLR/SYCP3 family and is an essential structural component of the transverse filaments of synaptonemal complexes formed between homologous chromosomes during meiotic prophase. Synaptonemal complex develops during prophase of first meiosis, wherein it allows the synapse as well as recombination between maternal - paternal chromosome, and SCP3 is essential to hold cohesin complex between homologous chromosomes, which avoid premature segregation thereby preventing aneuploidies. SCP3 is involved in phosphorylation of HORMAD1 and HORMAD2. It is essential to development of testis (where it is primarily localized) and defective SCP3 may leads to spermatogenic failure type 4 (SPGF4). SCP3 deificient mouse depicts high numbers of aneuploidies in oocytes and offspring. SCP3 is also expressed in certain malignancies including human acute lymphatic leukemias, cervical cancer cells, lung cancer cells etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SCP3/SYCP3 Antibody (NB300-231)(21)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ICC, ICC/IF, IHC, IHC-Fr, IHC-P, IP.


Filter By Application
ICC
(1)
ICC/IF
(7)
IHC
(2)
IHC-Fr
(1)
IHC-P
(2)
IP
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(13)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NB300-231 Applications Species
Wojtas MN, Pandey RR, Mendel M et al. Regulation of m(6)A Transcripts by the 3'-5' RNA Helicase YTHDC2 Is Essential for a Successful Meiotic Program in the Mammalian Germline. Mol. Cell. 2017 Oct 19 [PMID: 29033321] (Mouse) Mouse
Dai J, Voloshin O, Potapova S, Camerini-Otero RD. Meiotic Knockdown and Complementation Reveals Essential Role of RAD51 in Mouse Spermatogenesis. Cell Rep. 2017 Feb 07 [PMID: 28178517] (ICC/IF, Mouse) ICC/IF Mouse
Parvanov ED, Tian H, Billings T et al. PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis. Mol. Biol. Cell. Dec 8 2016 [PMID: 27932493] (IP, Mouse) IP Mouse
Ikegami K, Atsumi Y, Yorinaga E et al. Low temperature-induced circulating triiodothyronine accelerates seasonal testicular regression. Endocrinology. 2014 Nov 18 [PMID: 25406020] (IHC-P) IHC-P
Link J, Leubner M, Schmitt J et al. Analysis of Meiosis in SUN1 Deficient Mice Reveals a Distinct Role of SUN2 in Mammalian Meiotic LINC Complex Formation and Function. PLoS Genet. 2014 Mar 3 [PMID: 24586178] (IHC-P, Mouse) IHC-P Mouse
Lambeth LS, Cummins DM, Doran TJ et al. Overexpression of Aromatase Alone is Sufficient for Ovarian Development in Genetically Male Chicken Embryos. PLoS One. 2013 Jun 28 [PMID: 23840850]
Di Siena S, Campolo F, Rossi P et al. UV and genotoxic stress induce ATR relocalization in Mouse spermatocytes. Int J Dev Biol 2013 [PMID: 23784839] (ICC/IF, Mouse) ICC/IF Mouse
Di Giacomo M, Comazzetto S, Saini H et al. Multiple Epigenetic Mechanisms and the piRNA Pathway Enforce LINE1 Silencing during Adult Spermatogenesis. Mol Cell 2013 May 23 [PMID: 23706823] (ICC/IF, Mouse) ICC/IF Mouse
Tedesco M, Desimio MG, Klinger FG et al. Minimal Concentrations of Retinoic Acid Induce Stimulation by Retinoic Acid 8 and Promote Entry into Meiosis in Isolated Pregonadal and Gonadal Mouse Primordial Germ Cells. Biol Reprod 2013 Jun 13 [PMID: 23636811]
Kajiwara C, Kondo S, Uda S et al. Spermatogenesis arrest caused by conditional deletion of Hsp90alpha in adult mice. Biol Open 2012 Oct 15 [PMID: 23213375] (Mouse) Mouse
Show All 21 Publications.

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Product General Protocols

View specific protocols for SCP3/SYCP3 Antibody (NB300-231): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SCP3/SYCP3 Antibody (NB300-231). (Showing 1 - 1 of 1 FAQs).

  1. What Antigen Retrieval Methods do you use to do the Immunohistochemistry using NB300-231? What dilution do you used to stain the murine SCP3 in mouse ovary ?
    • This antibody was used at a dilution of 1:750 to stain the murine SCP3 protein in mouse ovary. Our lab most commonly uses HIER for antigen retrieval, please see this link, Antigen Retrieval Protocol.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-231AF405
Alexa Fluor 488 Labeled NB300-231AF488
Alexa Fluor 647 Labeled NB300-231AF647
Alexa Fluor 700 Labeled NB300-231AF700
Biotin Labeled NB300-231B
DyLight 405 Labeled NB300-231V
DyLight 488 Labeled NB300-231G
DyLight 550 Labeled NB300-231R
DyLight 650 Labeled NB300-231C
FITC Labeled NB300-231F
HRP Labeled NB300-231H

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Bioinformatics Tool for SCP3/SYCP3 Antibody (NB300-231)

Discover related pathways, diseases and genes to SCP3/SYCP3 Antibody (NB300-231). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on SCP3/SYCP3.

SCP3: A Key to Meiotic Recombination, Sterility and Cancer
Synaptonemal Complex Protein 3 (SCP3), which is a protein present in the synaptonemal complex which is responsible for pairing, synapsis, and recombination of homologous chromosomes during meiosis. Meiosis, in basic terms, is where germ cells divide t...  Read full blog post.

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Bioinformatics

Gene Symbol SYCP3
Entrez