Nestin Antibody (10C2) [FITC]

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Product Details

Summary
Reactivity Hu, Mu(-), Rb(-), Rt(-)Species Glossary
Applications Flow, ICC/IF, IHC-Fr, IHC-P
Clone
10C2
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Nestin Antibody (10C2) [FITC] Summary

Immunogen
A fragment of the human Nestin protein corresponding to residues 1464-1614. [UniProt P48681]
Localization
Cytoplasmic
Marker
Neural Stem Cell Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NES
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This Nestin (10C2) antibody is useful for Western blot, Immunofluorescence/Immunocytochemistry, Flow Cytometry, Immunohistochemistry on formaldehyde fixed cultured cells and formalin fixed paraffin-embedded tissue sections. In Western blot a doublet band is seen at ~220-240 kDa. The investigator should determine the optimal working dilution for a specific application.

Reactivity Notes

Human. It does not cross-react mouse, rat or rabbit.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Nestin Antibody (10C2) [FITC]

  • FLJ21841
  • NES
  • Nestin

Background

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Nestin Antibody (NB300-266F) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB300-266APC
Biotin Labeled NB300-266B
DyLight 350 Labeled NB300-266UV
DyLight 405 Labeled NB300-266V
DyLight 488 Labeled NB300-266G
DyLight 550 Labeled NB300-266R
DyLight 650 Labeled NB300-266C
DyLight 680 Labeled NB300-266FR
DyLight 755 Labeled NB300-266IR
HRP Labeled NB300-266H
PE Labeled NB300-266PE
PerCP Labeled NB300-266PCP

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Bioinformatics Tool for Nestin Antibody (NB300-266F)

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Research Areas for Nestin Antibody (NB300-266F)

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Blogs on Nestin.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including...  Read full blog post.

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Bioinformatics

Gene Symbol NES
Entrez