VEGF Antibody


Western Blot: VEGFA Antibody [NB100-2381] - Detection of VEGFA doublet in CSF-IR/VEGFA chimera transfected lysate using NB 100-2381. ECL 10 second exposure.

Product Details

Reactivity Hu, Mu, Rt, Bv, Ca, Ch, Eq, GPSpecies Glossary
Applications WB, ELISA
1.0 mg/ml

VEGF Antibody Summary

A synthetic peptide made to an internal region of the human VEGFA protein sequence (between residues 180-232). [Swiss-Prot# P15692]
Predicted Species
Primate (100%), Sheep (100%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate, pH 7-8
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


Application Notes
This VEGFA antibody is useful for Western blot analysis, where a band is seen at ~42 kDa with a chimera transfected lysate representing the homodimer VEGFA. Another faint band is seen at ~21 kDa, representing the monodomer form of VEGFA. This antibody did not work in IHC.
Control Peptide
VEGF Peptide (NB100-2381PEP)
Read Publication using NB100-2381.

Reactivity Notes

Predicted to react with sheep and primate based on 100% sequence homology.

Alternate Names for VEGF Antibody

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor


VEGFA (vascular endothelial growth factor A) belongs to the PDGF/VEGF growth factors family (VEGFA, VEGFB, VEGFC, VEGFD and PLGF) and it is the most studied growth factor which is essential for endothelial cell growth, embryonic vasculogenesis/angiogenesis and tumor angiogenesis. Members of VEGF family binds in an overlapping manner to VEGFR1/ FLT1, VEGFR2/KDR and VEGFR3, however, in vivo angiogenic response to VEGFA is primarily mediated via activation of VEGFR2 signaling. VEGFA exists as a disulfide-linked homodimer or as heterodimer with PGF, and besides VEGFR2, VEGFA can bind to VEGFR1, heparan sulfate, heparin etc., and its expression is regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations. VEGFA activates multiple signaling networks that promote endothelial cell growth, migration, differentiation, apoptotic resistance and vascular permeability. Defects in VEGFA are linked to MVCD1 (microvascular complications of diabetes type 1) and polymorphisms in VEGFA are associated with susceptibility to multiple cancers, e.g., glioma, HCC, ovarian, bladder, prostate, breast cancer etc.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGF Antibody (NB100-2381)(1)

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Product General Protocols

View specific protocols for VEGF Antibody (NB100-2381): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VEGF Antibody (NB100-2381). (Showing 1 - 3 of 3 FAQ).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.
  2. We are looking for a VEGFA antibody for use with porcine. Do you know if this antibody has actually been tested with porcine samples, or if cross-reactivity is just predicted based on homology? Is there any info or data regarding use with porcine?
    • The chimera transfected lysate shows on our website that is actually a porcine aorta endothelial cell line, which has been transfected with human VEGFR2. The antibody is detecting both the human protein and the endogenous porcine protein. We have received feedback from customers that NB100-2381 works in porcine tissues.
  3. I am looking for an antibody for detection of VEGFA in bovine samples in an ELISA-type assay. I found NB100-2381H. Do you know if this product has been specifically tested with bovine or if cross-reactivity is merely predicted based on homology? Also, do you know if ELISA in this case refers to detection of actual endogenous protein in a sample or if the assay was peptide-based?
    • We verified ELISA as an application based on a customer review. They used the antibody to identify the recombinant protein. It has not yet been tested to detect the endogenous protein in other samples like blood or plasma. Bovine is listed as reactive species based on homology, but we will fully guarantee detection of the bovine protein with this product. We will also guarantee the ELISA application. This will go for both the HRP conjugated antibody NB100-2381H and the unconjugated antibody NB100-2381.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-2381AF405
Alexa Fluor (R) 488 Labeled NB100-2381AF488
Alexa Fluor (R) 647 Labeled NB100-2381AF647
Alexa Fluor (R) 700 Labeled NB100-2381AF700
Biotin Labeled NB100-2381B
DyLight 405 Labeled NB100-2381V
DyLight 488 Labeled NB100-2381G
DyLight 550 Labeled NB100-2381R
DyLight 650 Labeled NB100-2381C
FITC Labeled NB100-2381F
HRP Labeled NB100-2381H

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Blogs on VEGF. Showing 1-10 of 12 blog posts - Show all blog posts.

Using SCP3/SYCP3 Antibodies as Meiosis Markers in Gametogenesis and DNA Repair Studies
The synaptonemal complex (SC) is a protein structure that forms during the synapsis of homologous chromosomes during meiosis. This structure is involved in the processes of chromosome synapsis, genetic recombination and subsequent chromosome segregati...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

Integrin alpha v beta 3 - a target for inhibiting tumor angiogenesis
Integrins are a family of transmembrane proteins involved in diverse processes including cell adhesion, signal transduction, cell migration, and differentiation. They exist as heterodimers consisting of noncovalently linked alpha and beta subunits....  Read full blog post.

HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.

HIF-1 alpha - sensing and responding to changing oxygen levels
Hypoxia-inducible factor 1 (HIF-1) allows cells to respond to changing levels of oxygen in the environment. HIF-1 is a heterodimeric transcription factor consisting of alpha and beta subunits. Under normal conditions HIF-1 alpha is continuously sy...  Read full blog post.

VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.

LYVE1 - It's Alive! It's a LYVE
LYVE1 (lymphatic vessel endothelial hyaluronan receptor-1) is one of the most specific and widely used mammalian lymphatic endothelial markers.It is found in lymph nodes and at the luminal/abluminal surfaces of lymphatic vessels. It is a single-pass t...  Read full blog post.

LOX: A prime enzyme
LOX is a copper-dependent amine oxidase enzyme that executes post-translational oxidative deamination on peptidyl lysine residues in precursors of fibrous collagen and elastin. LOX is secreted into the extracellular environment in an inactive form, wh...  Read full blog post.

VEGF Receptors, Angiogenesis and Cancer
Vascular endothelial growth factor receptors 1 and 2 (VEGFR1 and VEGFR2) are related family members of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. They are key regulators of physiological angiogenesis du...  Read full blog post.

Controlling the HIF-1 Switch
Hypoxia-inducible factor-1 is a major transcription factor composed of two subunits: HIF-1alpha and HIF-1 beta. Under normoxic conditions, HIF-1 alpha is targeted to proteosomal degradation via ubiquitination. On the other hand during hypoxic conditio...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

Contact Information

Product PDFs


Gene Symbol VEGFA

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