This VEGFA antibody is useful for Western blot analysis, where a band is seen at ~42 kDa with a chimera transfected lysate representing the homodimer VEGFA. Another faint band is seen at ~21 kDa, representing the monodomer form of VEGFA. This antibody did not work in IHC.
Predicted to react with sheep and primate based on 100% sequence homology.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
Immunogen affinity purified
Alternate Names for VEGF Antibody
vascular endothelial growth factor A
Vascular permeability factor
VPFvascular endothelial growth factor
VEGFA (vascular endothelial growth factor A) belongs to the PDGF/VEGF growth factors family (VEGFA, VEGFB, VEGFC, VEGFD and PLGF) and it is the most studied growth factor which is essential for endothelial cell growth, embryonic vasculogenesis/angiogenesis and tumor angiogenesis. Members of VEGF family binds in an overlapping manner to VEGFR1/ FLT1, VEGFR2/KDR and VEGFR3, however, in vivo angiogenic response to VEGFA is primarily mediated via activation of VEGFR2 signaling. VEGFA exists as a disulfide-linked homodimer or as heterodimer with PGF, and besides VEGFR2, VEGFA can bind to VEGFR1, heparan sulfate, heparin etc., and its expression is regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations. VEGFA activates multiple signaling networks that promote endothelial cell growth, migration, differentiation, apoptotic resistance and vascular permeability. Defects in VEGFA are linked to MVCD1 (microvascular complications of diabetes type 1) and polymorphisms in VEGFA are associated with susceptibility to multiple cancers, e.g., glioma, HCC, ovarian, bladder, prostate, breast cancer etc.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for VEGF Antibody (NB100-2381). (Showing 1 - 3 of 3 FAQs).
Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.
We are looking for a VEGFA antibody for use with porcine. Do you know if this antibody has actually been tested with porcine samples, or if cross-reactivity is just predicted based on homology? Is there any info or data regarding use with porcine?
The chimera transfected lysate shows on our website that is actually a porcine aorta endothelial cell line, which has been transfected with human VEGFR2. The antibody is detecting both the human protein and the endogenous porcine protein. We have received feedback from customers that NB100-2381 works in porcine tissues.
I am looking for an antibody for detection of VEGFA in bovine samples in an ELISA-type assay. I found NB100-2381H. Do you know if this product has been specifically tested with bovine or if cross-reactivity is merely predicted based on homology? Also, do you know if ELISA in this case refers to detection of actual endogenous protein in a sample or if the assay was peptide-based?
We verified ELISA as an application based on a customer review. They used the antibody to identify the recombinant protein. It has not yet been tested to detect the endogenous protein in other samples like blood or plasma. Bovine is listed as reactive species based on homology, but we will fully guarantee detection of the bovine protein with this product. We will also guarantee the ELISA application. This will go for both the HRP conjugated antibody NB100-2381H and the unconjugated antibody NB100-2381.
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LOX: A prime enzyme LOX is a copper-dependent amine oxidase enzyme that executes post-translational oxidative deamination on peptidyl lysine residues in precursors of fibrous collagen and elastin. LOX is secreted into the extracellular environment in an inactive form, wh... Read full blog post.
VEGF Receptors, Angiogenesis and Cancer Vascular endothelial growth factor receptors 1 and 2 (VEGFR1 and VEGFR2) are related family members of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. They are key regulators of physiological angiogenesis du... Read full blog post.
Controlling the HIF-1 Switch Hypoxia-inducible factor-1 is a major transcription factor composed of two subunits: HIF-1alpha and HIF-1 beta. Under normoxic conditions, HIF-1 alpha is targeted to proteosomal degradation via ubiquitination. On the other hand during hypoxic conditio... Read full blog post.
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