VEGF Antibody [DyLight 405]

Images

 
There are currently no images for VEGF Antibody (NB100-2381V).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, Eq, GP, Pm, ShSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 405

VEGF Antibody [DyLight 405] Summary

Immunogen
A synthetic peptide made to an internal region of the human VEGFA protein sequence (between residues 180-232). [Swiss-Prot# P15692]
Localization
Secreted
Predicted Species
Primate (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VEGFA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
This VEGFA antibody is useful for Western blot analysis, where a band is seen at ~42 kDa with a chimera transfected lysate representing the homodimer VEGFA. Another faint band is seen at ~21 kDa, representing the monodomer form of VEGFA. This antibody did not work in IHC.

Reactivity Notes

Predicted to react with sheep and primate based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for VEGF Antibody [DyLight 405]

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

VEGFA (vascular endothelial growth factor A) belongs to the PDGF/VEGF growth factors family (VEGFA, VEGFB, VEGFC, VEGFD and PLGF) and it is the most studied growth factor which is essential for endothelial cell growth, embryonic vasculogenesis/angiogenesis and tumor angiogenesis. Members of VEGF family binds in an overlapping manner to VEGFR1/ FLT1, VEGFR2/KDR and VEGFR3, however, in vivo angiogenic response to VEGFA is primarily mediated via activation of VEGFR2 signaling. VEGFA exists as a disulfide-linked homodimer or as heterodimer with PGF, and besides VEGFR2, VEGFA can bind to VEGFR1, heparan sulfate, heparin etc., and its expression is regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations. VEGFA activates multiple signaling networks that promote endothelial cell growth, migration, differentiation, apoptotic resistance and vascular permeability. Defects in VEGFA are linked to MVCD1 (microvascular complications of diabetes type 1) and polymorphisms in VEGFA are associated with susceptibility to multiple cancers, e.g., glioma, HCC, ovarian, bladder, prostate, breast cancer etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF644
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
NB100-105
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp, Ye
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, TCS, KO, LA
NB100-1642
Species: Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP, Flow-CS
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB110-41083
Species: Hu
Applications: WB, ChIP, ELISA
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-2381V
Species: Hu, Mu, Rt, Bv, Ca, Ch, Eq, GP, Pm, Sh
Applications: WB, ELISA

Publications for VEGF Antibody (NB100-2381V) (0)

There are no publications for VEGF Antibody (NB100-2381V).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for VEGF Antibody (NB100-2381V) (0)

There are no reviews for VEGF Antibody (NB100-2381V). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VEGF Antibody (NB100-2381V). (Showing 1 - 1 of 1 FAQ).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

Secondary Antibodies

 

Isotype Controls

Additional VEGF Products

Bioinformatics Tool for VEGF Antibody (NB100-2381V)

Discover related pathways, diseases and genes to VEGF Antibody (NB100-2381V). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for VEGF Antibody (NB100-2381V)

Discover more about diseases related to VEGF Antibody (NB100-2381V).
 

Pathways for VEGF Antibody (NB100-2381V)

View related products by pathway.

PTMs for VEGF Antibody (NB100-2381V)

Learn more about PTMs related to VEGF Antibody (NB100-2381V).
 

Research Areas for VEGF Antibody (NB100-2381V)

Find related products by research area.

Blogs on VEGF. Showing 1-10 of 16 blog posts - Show all blog posts.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit. ...  Read full blog post.

Using SCP3/SYCP3 Antibodies as Meiosis Markers in Gametogenesis and DNA Repair Studies
The synaptonemal complex (SC) is a protein structure that forms during the synapsis of homologous chromosomes during meiosis. This structure is involved in the processes of chromosome synapsis, genetic recombination and subsequent chromosome segregati...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

Integrin alpha v beta 3 - a target for inhibiting tumor angiogenesis
Integrins are a family of transmembrane proteins involved in diverse processes including cell adhesion, signal transduction, cell migration, and differentiation. They exist as heterodimers consisting of noncovalently linked alpha and beta subunits....  Read full blog post.

HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.

HIF-1 alpha - sensing and responding to changing oxygen levels
Hypoxia-inducible factor 1 (HIF-1) allows cells to respond to changing levels of oxygen in the environment. HIF-1 is a heterodimeric transcription factor consisting of alpha and beta subunits. Under normal conditions HIF-1 alpha is continuously sy...  Read full blog post.

VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.

Showing 1-10 of 16 blog posts - Show all blog posts.

Customers Who Bought This Also Bought

VEGF Blocking Peptide
NB100-2381PEP
VEGF [Unconjugated]
293-VE-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our VEGF Antibody [DyLight 405] and receive a gift card or discount.

Bioinformatics

Gene Symbol VEGFA
Entrez