TRIAD3 Antibody - BSA Free

Western Blot: TRIAD3 Antibody [NB100-56891] - Analysis of TRIAD3 in human (A) Brain; (B) Heart; (C) Small Intestine; (D) Kidney; (E) Liver; (F) Lung; (G) Skeletal Muscle; (H) Pancreas; (I) Spleen; (J) Ovary; (K) Testis using NB100-56891 at 1 ug/mL.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

TRIAD3 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TRIAD3 between amino acids 350-400.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNF216
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 3 ug/mL
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)
  Human Ovary Whole Tissue Lysate (Adult Whole Normal)
  Human Spleen Whole Tissue Lysate (Adult Whole Normal)
  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
  Human Small Intestine Whole Tissue Lysate (Adult Whole Normal)
  Human Kidney Whole Tissue Lysate (Adult Whole Normal)
  Human Pancreas Whole Tissue Lysate (Adult Whole Normal)
  TRIAD3 Overexpression Lysate
  Human Heart Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for TRIAD3 Antibody - BSA Free

• EC 6.3.2.-
• ring finger protein 216Ubiquitin-conjugating enzyme 7-interacting protein 1
• Triad domain-containing protein 3
• TRIAD3Zinc finger protein inhibiting NF-kappa-B
• U7I1
• UBCE7IP1ubiquitin conjugating enzyme 7 interacting protein 1
• ZINE3 ubiquitin-protein ligase RNF216

Background

Official Gene Symbol: TRIAD3 Gene ID: 54476 (Human) Gene Map Locus: 7p22.1 (Human) TRIADs are novel zinc finger proteins characterized by the presence of Cysteine rich TRIAD domain consisting of DRIL motif flanked by 2 RING fingers. TRIAD3, a 56KDa protein, is a novel member of this family. It contains 4 RLD domains in the centre and a proline-rich domain at the C-terminus. Localized in the cytoplasm, it interacts with RIP and inhibits TNF and IL1-induced NF-kappaB activation pathways. Reports suggest TRIAD3 acts as an E3-ubiquitin-protein ligase and promotes ubiquitination and degradation of TLR4 and TLR9, thereby regulating TLR signaling. It also interacts with HIV type I Vif protein and interferes in the early events of HIV-1 replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF216
• Entrez:
  • 54476(Human)
• Uniprot:
  • NP_996999(Human)