APOBEC3G Antibody

Images

 
Western Blot: APOBEC3G Antibody [NBP1-77206] - Caco-2 cell lysate in the (A), absence and (B) presence of blocking peptide with APOBEC3G antibody at 5 ug/ml..
Immunohistochemistry-Paraffin: APOBEC3G Antibody [NBP1-77206] - Staining of APOBEC3G expression in Caco-2 cell lysate in the (A), absence and (B) presence of blocking peptide with anti-APOBEC3G (NT) at 5 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

APOBEC3G Antibody Summary

Immunogen
Antibody was raised against a synthetic peptide corresponding to 15 amino acids near the amino-terminus of human APOBEC3G. The immunogen is located within the first 50 amino acids of APOBEC3G.
Specificity
APOBEC3G antibody will also detect the APOBEC3F isoform.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APOBEC3G
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence
Application Notes
APOBEC3G antibody can be used for detection of APOBEC3G by Western blot at 5 mg/ml.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for APOBEC3G Antibody

  • APOBEC-related cytidine deaminase
  • APOBEC-related protein 9
  • APOBEC-related protein
  • apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
  • ARCD
  • ARP-9
  • bK150C2.7
  • CEM-15
  • CEM15ARP9
  • dJ494G10.1
  • DNA dC->dU-editing enzyme APOBEC-3G
  • EC 3.5.4
  • EC 3.5.4.-
  • EC 3.5.4.5
  • FLJ12740DNA dC->dU editing enzyme
  • MDS019
  • phorbolin-like protein MDS019

Background

The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5alpha which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol APOBEC3G
Uniprot