RIPK1/RIP1 Antibody - BSA Free

Images

 
Biological Strategies: Western Blot: RIPK1/RIP1 Antibody [NBP1-77077] - At 6 h after CCI, RIP1 protein levels in the cortex detected by western blotting were decreased in Nec-1 and melatonin pretreatment groups, ...read more
Immunocytochemistry/ Immunofluorescence: RIPK1/RIP1 Antibody [NBP1-77077] - Mouse Kidney cells with RIPK1 antibody at 20 ug/mL.
Immunohistochemistry: RIPK1/RIP1 Antibody [NBP1-77077] - Traumatic brain injury (TBI) tissues show increased necroptosis compared with normal brain tissues (NBTs). The expression of RIP1 was tested in NBT and TBI ...read more
Western Blot: RIPK1/RIP1 Antibody [NBP1-77077] - Rat kidney tissue lysate with RIPK1 antibody at 1 ug/mL.
Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-77077] - Mouse kidney tissue with RIPK1 antibody at 2.5 ug/ml.
Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-77077] - Staining of mouse kidney tissue with antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

RIPK1/RIP1 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human RIPK1. The immunogen is located within amino acids 180 - 230 of RIPK1. Amino Acid Squence: DVNAKPTEKSDVYS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RIPK1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Knockdown Validated
  • Western Blot 1 ug/ml
Theoretical MW
70.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
RIPK1/RIP1 Peptide (NBP1-77077PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-77077 in the following applications:

Publications
Read Publications using
NBP1-77077 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • 2 publications
  • KD
    1 publication
  • WB
    7 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for RIPK1/RIP1 Antibody - BSA Free

  • Cell death protein RIP
  • EC 2.7.11.1
  • receptor (TNFRSF)-interacting serine-threonine kinase 1
  • receptor interacting protein
  • Receptor-interacting protein 1
  • receptor-interacting serine/threonine-protein kinase 1
  • RIP
  • Rip1 kinase
  • RIP1
  • RIP-1
  • RIPFLJ39204
  • RIPK1
  • Serine/threonine-protein kinase RIP

Background

RIPK1 (Receptor Interacting Protein) is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell's decision to live or die. RIPK1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIPK1 is important for signallling to NF-kappa-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIPK1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-kappaB activation. RIPK1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-77077
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, KD

Publications for RIPK1/RIP1 Antibody (NBP1-77077)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, KD, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
IHC-P
(2)
KD
(1)
WB
(7)
All Applications
Filter By Species
Human
(2)
Mouse
(6)
Rat
(3)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NBP1-77077 Applications Species
Lorenzo N, Sanavia T, Rocco C et al. Necroptosis driving genes RIPK1, RIPK3, and MLKL-p are associated with intratumoral CD3+ and CD8+ T-cell density and predict prognosis in Hepatocellular Carcinoma Journal for ImmunoTherapy of Cancer Jan 1 2022 [PMID: 35264437]
Lorenzo N, Sanavia T, Rocco C et al. Necroptosis-driving genes RIPK1, RIPK3 and MLKL-p are associated with intratumoral CD3+ and CD8+ T cell density and predict prognosis in hepatocellular carcinoma Journal for immunotherapy of cancer Jan 1 2022 [PMID: 35264437]
Kamiya M, Mizoguchi F, Kawahata K et al. Targeting necroptosis in muscle fibers ameliorates inflammatory myopathies Nature communications Jan 10 2022 [PMID: 35013338] (ICC/IF, Mouse) ICC/IF Mouse
Pesce NA, Canovai A, Plastino F Et al. An imbalance in autophagy contributes to retinal damage in a rat model of oxygen-induced retinopathy Journal of cellular and molecular medicine Oct 8 2021 [PMID: 34623024] (WB, ICC/IF, Rat) WB, ICC/IF Rat
Naseroleslami M, Niri NM, Akbarzade I et al. Simvastatin-loaded nano-niosomes confer cardioprotection against myocardial ischemia/reperfusion injury Drug delivery and translational research Jun 24 2021 [PMID: 34165730]
Sharifi M, Nazarinia D, Ramezani F et al. Necroptosis and RhoA/ROCK pathways: molecular targets of Nesfatin-1 in cardioprotection against myocardial ischemia/reperfusion injury in a rat model Molecular biology reports Mar 23 2021 [PMID: 33755849]
Zhao Y, Zhu X, Zhang L et al. Mesenchymal stem/stromal cells and their extracellular vesicle progeny decrease injury in post-stenotic swine kidney through different mechanisms Stem Cells Dev. Jul 12 2020 [PMID: 32657229]
Li J, Shi J, Pan Y et al. Transcription modulation by CDK9 regulates inflammatory genes and RIPK3-MLKL-mediated necroptosis in periodontitis progression Sci Rep. Nov 22 2019 [PMID: 31758083] (WB, Mouse) WB Mouse
Du XK, Ge WY, Jing R, Pan LH Necroptosis in pulmonary macrophages mediates lipopolysaccharide-induced lung inflammatory injury by activating ZBP-1 Int. Immunopharmacol. 2019 Oct 23 [PMID: 31655343] (WB, KD, Mouse) WB, KD Mouse
Bao Z, Fan L, Zhao L et al. Silencing of A20 Aggravates Neuronal Death and Inflammation After Traumatic Brain Injury: A Potential Trigger of Necroptosis Front. Mol. Neurosci. Sep 19 2019 [PMID: 31607859] (WB, Rat) WB Rat
Show All 17 Publications.

Review for RIPK1/RIP1 Antibody (NBP1-77077) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-77077:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IHC-P Mouse 12/24/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 24095740
SpeciesMouse
Special ApplicationsSee PMID 24095740

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RIPK1/RIP1 Antibody (NBP1-77077) (0)

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Secondary Antibodies

 

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Recent Reviews

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Verified Customer
12/24/2014
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol RIPK1
Entrez
Uniprot