RIPK1/RIP1 Antibody

Images

 
Western Blot: RIPK1/RIP1 Antibody [NBP1-77077] - Rat kidney tissue lysate with RIPK1 antibody at 1 ug/mL.
Immunohistochemistry: RIPK1/RIP1 Antibody [NBP1-77077] - Staining of mouse kidney tissue with antibody at 2.5 ug/ml.
Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-77077] - Mouse kidney tissue with RIPK1 antibody at 2.5 ug/ml.
Immunofluorescence: RIPK1/RIP1 Antibody [NBP1-77077] - Mouse Kidney cells with RIPK1 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, ICC/IF, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

RIPK1/RIP1 Antibody Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human RIPK1. The immunogen is located within amino acids 180 - 230 of RIPK1. Amino Acid Squence: DVNAKPTEKSDVYS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RIPK1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Immunofluorescence 20 ug/ml
  • Knockdown Validated
Theoretical MW
70.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
RIPK1/RIP1 Peptide (NBP1-77077PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-77077 in the following applications:

Publications
Read Publications using
NBP1-77077 in the following applications:

  • IHC
    1 publication
  • 2 publications
  • KD
    1 publication
  • WB
    6 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for RIPK1/RIP1 Antibody

  • Cell death protein RIP
  • EC 2.7.11.1
  • receptor (TNFRSF)-interacting serine-threonine kinase 1
  • receptor interacting protein
  • Receptor-interacting protein 1
  • receptor-interacting serine/threonine-protein kinase 1
  • RIP
  • Rip1 kinase
  • RIP1
  • RIP-1
  • RIPFLJ39204
  • RIPK1
  • Serine/threonine-protein kinase RIP

Background

RIPK1 (Receptor Interacting Protein) is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell's decision to live or die. RIPK1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIPK1 is important for signallling to NF-kappa-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIPK1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-kappaB activation. RIPK1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, IHC, IHC-P, ICC/IF, KD

Publications for RIPK1/RIP1 Antibody (NBP1-77077)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: IHC, IHC-P, KD, WB.


Filter By Application
IHC
(1)
IHC-P
(2)
KD
(1)
WB
(6)
All Applications
Filter By Species
Human
(2)
Mouse
(5)
Rat
(2)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-77077 Applications Species
Zhao Y, Zhu X, Zhang L et al. Mesenchymal stem/stromal cells and their extracellular vesicle progeny decrease injury in post-stenotic swine kidney through different mechanisms Stem Cells Dev. Jul 12 2020 [PMID: 32657229]
Li J, Shi J, Pan Y et al. Transcription modulation by CDK9 regulates inflammatory genes and RIPK3-MLKL-mediated necroptosis in periodontitis progression Sci Rep. Nov 22 2019 [PMID: 31758083] (WB, Mouse) WB Mouse
Du XK, Ge WY, Jing R, Pan LH Necroptosis in pulmonary macrophages mediates lipopolysaccharide-induced lung inflammatory injury by activating ZBP-1 Int. Immunopharmacol. 2019 Oct 23 [PMID: 31655343] (WB, KD, Mouse) WB, KD Mouse
Tsuiji H, Inoue I, Takeuchi M et al. TDP-43 accelerates age-dependent degeneration of interneurons Sci Rep. 2017 Nov 02 [PMID: 29097807] (IHC-P, Mouse)

Details:
Novus RIPK1 IHC antibody was used to investigate necroptosis in TDP-43 Tg mice
IHC-P Mouse
Al-Lamki RS, Lu W, Manalo P et al. Tubular epithelial cells in renal clear cell carcinoma express high RIPK1/3 and show increased susceptibility to TNF receptor 1-induced necroptosis Cell Death Dis 2016 Jul 01 [PMID: 27362805]
Miki Y, Akimoto J, Moritake K et al. Photodynamic therapy using talaporfin sodium induces concentration-dependent programmed necroptosis in human glioblastoma T98G cells Lasers Med Sci. 2015 Jun 25 [PMID: 26109138] (WB, Human)

Details:
human glioblastoma multiforma tumor cell line T98G treated with Control siRNA or (Fig. 4)
WB Human
Nilsson P, Loganathan K, Sekiguchi M et al. AB secretion and plaque formation depend on autophagy Cell Rep. 2013 Oct 17 [PMID: 24095740] (IHC-P, Mouse) IHC-P Mouse
Liu H, Deng X, Zhang L et al. Knockdown RIP-1 inhibits production of oxLDL-induced inflammatory cytokines and promotes ABCA1 expression in macrophages. Int J Clin Exp Med (WB, Mouse) WB Mouse
Bao Z, Fan L, Zhao L et al. Silencing of A20 Aggravates Neuronal Death and Inflammation After Traumatic Brain Injury: A Potential Trigger of Necroptosis Front. Mol. Neurosci. Sep 19 2019 [PMID: 31607859] (WB, Rat) WB Rat
Wu Z, Fournel L, Stadler N et al. Modulation of lung cancer cell plasticity and heterogeneity with the restoration of cisplatin sensitivity by Neurotensin antibody Cancer Lett. Dec 21 2018 [PMID: 30583074] (IHC, Human) IHC Human
Show All 11 Publications.

Review for RIPK1/RIP1 Antibody (NBP1-77077) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-77077:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Mouse 12/24/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 24095740
SpeciesMouse
Special ApplicationsSee PMID 24095740

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Recent Reviews

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5
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4
1
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1
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Anonymous
12/24/2014
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol RIPK1
Entrez
Uniprot