RPAIN Antibody

Images

 
Western Blot: RPAIN Antibody [NBP1-76749] - Jurkat cell lysate with RPAIN antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry-Paraffin: RPAIN Antibody [NBP1-76749] - Mouse stomach tissue with RPAIN antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

RPAIN Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RPA IP. The immunogen is located within the last 50 amino acids of RPA Interacting Protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPAIN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody is useful in ELISA, Western Blot, Immunohistochemistry-Paraffin. RPA Interacting Protein antibody can be used for detection of RPA Interacting Protein by Western blot at 0.5 - 1 mg/ml.
Control Peptide
RPAIN Peptide (NBP1-76749PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RPAIN Antibody

  • FLJ25625
  • FLJ30490
  • FLJ42429
  • hRIP
  • MGC4189
  • nuclear transporter
  • RAP interaction protein
  • RIPHRIP
  • RPA interacting protein
  • RPA-interacting protein

Background

Replication protein A (RPA) is a single-stranded-DNA binding protein involved in numerous eukaryotic DNA processes including replication, repair and recombination. RPA interacting protein (RPA IP) has been identified as an adapter protein that is involved in RPA nuclear import instead of the prototypical importin proteins that normally mediate nuclear import. Multiple isoforms of RPA IP are known to exist, with the longest isoform localized to the cytoplasm. Isoform 2 is sumoylated and is located in the PML nuclear body within the nucleus. It has been suggested that this isoform mediates the localization of the RPA complex into the PML nuclear body, thereby participating in RPA function in DNA metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RPAIN
Uniprot