Growth Hormone Antibody [Unconjugated]

Western blot shows lysates of human, mouse, and rat pituitary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Growth Hormone at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Growth Hormone was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Recombinant Human Growth Hormone (Catalog # 1067-GH) stimulates proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Growth Hormone (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067). The ND50 is typically 1.5-7.5 ng/mL.

Recombinant Human Growth Hormone protein was serially diluted 2-fold and captured by Mouse Anti-Human Growth Hormone Monoclonal Antibody (Catalog # MAB10671) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Growth Hormone Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Growth Hormone
  Phe27-Phe217
  Accession # CAA23779
• Specificity: Detects human Growth Hormone in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GH1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 1.5-7.5 ng/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Growth Hormone Antibody [Unconjugated]

• GH1
• GHB5
• GHIGHD1B
• GHN
• GH-N
• GHNGrowth hormone
• GH-Nsomatotropin
• growth hormone 1Pituitary growth hormone
• Growth Hormone
• hGH-N
• IGHD1A
• IGHD2
• Somatotropin

Background

Growth Hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretary granules. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones - GH-releasing hormone (GHRH) and somatostatin (SST) - as well as by signals from the periphery - ghrelin (3) and leptin (4). The human GH cDNA encodes a 217 amino acid (aa) residue precursor protein with a 26 aa putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified (5).

Human GH is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor, which is present in many cell types (1, 2). GH stimulates the liver and other tissues to produce IGF-I, which regulates growth and metabolism. GH has also been shown to have direct effects on growth that is independent of IGF-I. GH, directly or indirectly via IGF-I, can act on B cells, T cells, NK cells, macrophages, and neutrophils to exert immunomodulatory activities (6). In addition, GH can act directly on various cell types to induce lipolysis, lactation, amino acid uptake, and protein synthesis (1, 2, 6).

1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
2. Le Roith, D. et al. (2001) Endocrine Rev. 22:53.
3. Kojima, K. et al. (1999) Nature, 402:656.
4. Tannenbaum, G. et al. (1998) Endocrinol. 139:3871.
5. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Growth Hormone Antibody (AF1067)(9)

Publications using AF1067

• V Chesnokova, S Zonis, A Apostolou, HQ Estrada, S Knott, K Wawrowsky, K Michelsen, A Ben-Shlomo, R Barrett, V Gorbunova, K Karalis, S Melmed Local non-pituitary growth hormone is induced with aging and facilitates epithelial damage Cell Reports, 2021-12-14;37(11):110068. 2021-12-14 [PMID: 34910915] (Western Blot, Human)
• EJ Vélez, S Unniappan Nesfatin-1 and nesfatin-1-like peptide suppress growth hormone synthesis via the AC/PKA/CREB pathway in mammalian somatotrophs Sci Rep, 2020-10-07;10(1):16686. 2020-10-07 [PMID: 33028951] (Western Blot, Rat)
• D Abboud, AF Daly, N Dupuis, MA Bahri, A Inoue, A Chevigné, F Ectors, A Plenevaux, B Pirotte, A Beckers, J Hanson GPR101 drives growth hormone hypersecretion and gigantism in mice via constitutive activation of Gs and Gq/11 Nat Commun, 2020-09-21;11(1):4752. 2020-09-21 [PMID: 32958754] (IHC, Mouse)
• H Sasanuma, M Nakata, K Parmila, J Nakae, T Yada PDK1-FoxO1 pathway in AgRP neurons of arcuate nucleus promotes bone formation via GHRH-GH-IGF1 axis Mol Metab, 2017-02-17;6(5):428-439. 2017-02-17 [PMID: 28462077] (IHC-P, Mouse)
• Growth hormone is permissive for neoplastic colon growth Proc Natl Acad Sci USA, 2016-05-25;0(0):. 2016-05-25 [PMID: 27226307] (Western Blot, Human)
• Zhou , Cuiqi, Jiao , Yonghui, Wang , Renzhi, Ren , Song-Gua, Wawrowsky , Kolja, Melmed , Shlomo STAT3 upregulation in pituitary somatotroph adenomas induces growth hormone hypersecretion. J Clin Invest, 2015-03-16;125(4):1692-702. 2015-03-16 [PMID: 25774503] (Western Blot, Human)
• Myler HA, McVay S, Kratzsch J Troubleshooting PEG-hGH detection supporting pharmacokinetic evaluation in growth hormone deficient patients. J Pharmacol Toxicol Methods, 2010-01-04;61(2):92-7. 2010-01-04 [PMID: 20044010] (Electrochemiluminescent Assay, Human)
• Harvey S, Parker E, Macdonald I, Sanders EJ Growth hormone is present in the human retina and vitreous fluid. Neurosci. Lett., 2009-03-27;455(3):199-202. 2009-03-27 [PMID: 19429121] (IHC-P, Western Blot, Human)
• Lange T, Dimitrov S, Fehm HL, Westermann J, Born J Shift of monocyte function toward cellular immunity during sleep. Arch. Intern. Med., 2006-09-18;166(16):1695-700. 2006-09-18 [PMID: 16983046] (Neutralization, Human)

Bioinformatics

• Gene Symbol: GH1
• Uniprot:
  • CAA23779()