KIR2DL4/CD158d Antibody (181703)

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Human KIR2DL4/CD158d Monoclonal Antibody induces IFN‑ gamma secretion in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner, as measured using the Quantikine Human IFN‑ gamma ELISA Kit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-reported, Func
Clone
181703
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

KIR2DL4/CD158d Antibody (181703) Summary

Immunogen
BaF3 mouse pro-B cell line transfected with human KIR2DL4/CD158d
Accession # Q99706
Specificity
Stains human KIR2DL4/CD158d transfected BaF/3 cells. It does not recognize transfectants that express KIR2DL1, 2DL2, 2DL3, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
KIR2DL4
Purity
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Agonist Activity 0.5-2 ug/mL
  • CyTOF-reported
Publications
Read Publications using
MAB2238 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KIR2DL4/CD158d Antibody (181703)

  • 103AS
  • 15.212
  • CD158 antigen-like family member D
  • CD158d antigen
  • CD158d
  • CD158DKIR103ASKiller cell inhibitory receptor 103AS
  • G9P
  • killer cell immunoglobulin-like receptor 2DL4
  • killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4
  • killer Ig receptor
  • KIR103
  • KIR-103AS
  • KIR2DL4
  • MHC class I NK cell receptor KIR103AS
  • natural killer cell inhibitory receptor
  • NK cell receptor

Background

KIR2DL4 (also known as 2DL4, p49, CD158d, KIR103) is a type I transmembrane protein of the killer cell Ig-like receptor (KIR) family expressed on NK and subsets of gamma δT and memory/effector alpha beta T cells. KIR2DL4 is a unique KIR (1-3); alleles are not clonally restricted but are expressed codominantly (4) in all activated NK cells and constitutively on CD56hi NK cells. KIR members with two Ig-like domains (2D) usually express domains D1 and D2, but KIR2DL4 expresses D0 and D2. Other long-tailed (L) KIR have two cytoplasmic inhibitory signaling domains (ITIM), but KIR2DL4 has one ITIM and also exhibits characteristics of activating KIR (2). An arginine within the transmembrane sequence of KIR2DL4 interacts with the signaling molecule Fc epsilon RI-gamma, while in activating KIR, a transmembrane lysine interacts with DAP12 (1, 5). The KIR2DL4 gene is highly polymorphic. Seven splice variants missing one or more exons have been identified, but it is not clear whether these are expressed. Several of the nine alleles identified encode a frameshift creating a prematurely truncated protein. It is estimated that up to 25% of humans do not express KIR2DL4 capable of reaching the cell surface (1, 7, 10). Human KIR2DL4 is 65-83% amino acid identical to other primates. KIR receptors have no structural orthologs in non-primates, although mouse Ly49 proteins are functional orthologs. Cross-linking of KIR2DL4 induces NK cells to produce IFN-gamma (6, 7); stimulation with IL-2 upregulates cell surface expression on CD56dim cells and allows cytotoxicity (7). Although a role in immune privilege of the fetus has been suggested due to reported recognition of fetal trophoblast HLA-G by KIR2DL4 in the maternal decidua (11), subsequent data have not supported this recognition (1, 9).

  1. Lanier, L.L. (2005) Annu. Rev. Immunol. 23:225.
  2. Faure, M. and E.O. Long (2002) J. Immunol. 168:6208.
  3. Selvakumar, A. et al. (1996) Tissue Antigens 48:285.
  4. Chan, H-W. et al. (2003) J. Exp. Med. 197:245.
  5. Kikuchi-Maki, A. et al. (2005) J. Immunol. 174:3859.
  6. Rajagopalan, S. et al. (2001) J. Immunol. 167:1877.
  7. Kikuchi-Maki, A. et al. (2003) J. Immunol. 171:3415.
  8. Gedil, M.A. et al. (2005) Tissue Antigens 65:402.
  9. Witt, C.S. et al. (2002) Eur. J. Immunol. 32:18.
  10. Goodridge, J.P. et al. (2003) J. Immunol. 171:1768.
  11. Ponte, M. et al. (1999) Proc. Natl. Acad. Sci. USA 96:5674.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KIR2DL4/CD158d Antibody (MAB2238)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Flow, Functional Assay, ICC, IP.


Filter By Application
Flow
(3)
Functional Assay
(1)
ICC
(1)
IP
(1)
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Filter By Species
Human
(6)
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Showing Publications 1 - 6 of 6.
Publications using MAB2238 Applications Species
Y Takei, C Ueshima, TR Kataoka, M Hirata, A Sugimoto, M Rokutan-Ku, K Moriyoshi, K Ono, I Murakami, S Iwamoto, H Haga Killer cell immunoglobulin-like receptor 2DL4 is expressed in and suppresses the cell growth of Langerhans cell histiocytosis Oncotarget, 2017;8(23):36964-36972. 2017 [PMID: 28445138] (Functional Assay,, Human) Functional Assay Human
Ashley Moffett Activating KIR2DS4 Is Expressed by Uterine NK Cells and Contributes to Successful Pregnancy J. Immunol., 2016;0(0):. 2016 [PMID: 27815424] (Flow,, Human) Flow Human
Rajagopalan S, Lee E, DuPrie M, Long E TNFR-associated factor 6 and TGF-beta-activated kinase 1 control signals for a senescence response by an endosomal NK cell receptor. J Immunol, 2014;192(2):714-21. 2014 [PMID: 24337384] (IP,, Human) IP Human
Le Page M, Goodridge J, John E, Christiansen F, Witt C Killer Ig-like receptor 2DL4 does not mediate NK cell IFN-gamma responses to soluble HLA-G preparations. J Immunol, 2014;192(2):732-40. 2014 [PMID: 24337374] (Flow,, Human) Flow Human
Siewiera J, El Costa H, Tabiasco J, Berrebi A, Cartron G, Le Bouteiller P, Jabrane-Ferrat N Human cytomegalovirus infection elicits new decidual natural killer cell effector functions. PLoS Pathog, 2013;9(4):e1003257. 2013 [PMID: 23592985] (Flow,, Human) Flow Human
Esin S, Batoni G, Counoupas C, Stringaro A, Brancatisano FL, Colone M, Maisetta G, Florio W, Arancia G, Campa M Direct binding of human NK cell natural cytotoxicity receptor NKp44 to the surfaces of mycobacteria and other bacteria. Infect. Immun., 2008;76(4):1719-27. 2008 [PMID: 18212080] (ICC,, Human) ICC Human

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Bioinformatics

Gene Symbol KIR2DL4
Entrez
Uniprot