Human TNF RI/TNFRSF1A Quantikine ELISA Kit

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Human TNF RI/TNFRSF1A Quantikine ELISA Kit Summary

Background
The Quantikine Human sTNF RI Immunoassay is a 4.5 hour solid phase ELISA designed to measure sTNF RI in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human sTNF RI, as well as antibodies raised against this polypeptide. The recombinant protein represents the non-glycosylated, N-terminal methionyl form of the naturally occurring human so...luble Type I receptor for TNF with an apparent molecular weight of approximately 18.6 kDa. This immunoassay has been shown to accurately quantitate the recombinant sTNF RI. Results obtained on samples containing natural sTNF RI showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural sTNF RI. Since the measurement of human sTNF RI by this immunoassay is relatively insensitive to added TNF-alpha or TNF-beta, it is probable that this measurement corresponds to the total amount of the soluble receptor present in samples (i.e. the total amount of free receptor plus the total amount of receptor bound to TNF).
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Specificity
Natural and recombinant human sTNF RI
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
TNFRSF1A

Applications/Dilutions

Application Notes
Interference observed with 1 or more available related molecules.
Publications
Read Publications using DRT100.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TNF RI/TNFRSF1A Quantikine ELISA Kit

  • CD120a antigen
  • CD120a
  • FPF
  • p55
  • p55-R
  • p60
  • TNF RI
  • TNFARMGC19588
  • TNF-R
  • TNF-R1
  • TNFR1TBP1
  • TNFR55
  • TNF-R55
  • TNFR60
  • TNFRI
  • TNF-RI
  • TNF-R-I
  • TNFR-I
  • TNFRSF1A
  • tumor necrosis factor binding protein 1
  • Tumor necrosis factor receptor 1
  • tumor necrosis factor receptor 1A isoform beta
  • tumor necrosis factor receptor superfamily member 1A
  • tumor necrosis factor receptor superfamily, member 1A
  • tumor necrosis factor receptor type 1
  • Tumor necrosis factor receptor type I
  • tumor necrosis factor-alpha receptor

Background

Tumor necrosis factors (TNFs) are pleiotropic cytokines that are considered primary modifiers of the inflammatory and immune reactions of animals produced in response to injury or infection. Two forms of TNF, designated TNF-alpha (or cachectin) and TNF-beta (or lymphotoxin), have been described that share 30% sequence similarity and compete for binding to the same receptors. TNFs play a necessary and beneficial role as mediators of host resistance to infections and tumor formation. However, over-production or inappropriate expression of these factors can lead to a variety of pathological conditions, including wasting, systemic toxicity, and septic shock. For reviews of the literature relating to these factors, see references 1 and 2. 
The actions of TNFs are produced subsequent to binding of the factors to cell surface receptors. Two distinct TNF receptors have been identified and cloned. Virtually all cell types studied show the presence of one or both of these receptor types. One receptor type, termed TNF RII (Type A, Type a, 75 kDa or utr antigen), shows an apparent molecular weight of 75 kDa. The gene for this receptor encodes a presumptive transmembrane protein of 439 amino acid (aa) residues (3, 19). The other receptor type, termed TNF RI (Type B, Type b, 55 kDa or htr antigen), shows an apparent molecular weight of 55 kDa. The gene for this protein encodes a transmembrane protein of 426 aa residues (4, 5, 19). Both receptor types show high affinity binding of either TNF-alpha or TNF-beta. The two receptor types are immunologically distinct but their extracellular domains show similarities in the pattern of cysteine residue locations in four domains (3). The intracellular domains of the two receptor types are apparently unrelated, suggesting the possibility that the two receptor types employ different signal transduction pathways. 
Several groups have identified soluble TNF binding proteins in human serum and urine (6-8) that can neutralize the biological activities of TNF-alpha and TNF-beta. Two types have been identified and designated sTNF RI (or TNF BPI) and sTNF RII (or TNF BPII). These soluble forms have now been shown to represent truncated forms of the two types of TNF receptors discussed above. The soluble receptor forms apparently arise as a result of shedding of the extracellular domains of the receptors, and concentrations of about 1-2 ng/mL are found in the serum and urine of healthy subjects (9, 10). The levels of the soluble receptors vary from individual to individual but are stable over time for given individuals (9). 
Elevated levels of TNF receptors have been found in the amniotic fluid and urine of pregnant women (11), in serum or plasma in association with pathological conditions such as endotoxinemia (12, 13), meningiococcemia (14), and HIV infection (15), and in plasma and ascites of patients in association with infections and malignancies (16). The mechanisms involved in the induction of shedding of the TNF receptors are not well understood. There are reports of correlations between increased TNF levels and soluble receptor levels, suggesting generally that stimuli that cause TNF levels to rise also induce shedding of TNF receptors (12-14, 17). There is also evidence, however, that suggests the shedding of the two types of soluble receptors is independently regulated (13). 2 For research use only. Not for use in diagnostic procedures. 
The physiological role of the soluble TNF receptors is not known. It is known that both types of soluble receptors can bind to TNF in vitro and inhibit its biological activity by competing with cell surface receptors for TNF binding. Consequently it has been suggested that shedding of soluble receptors in response to TNF release could serve as a mechanism for binding and inhibiting the TNF not immediately bound to surface receptors, thus protecting other cells from the effects of TNF and localizing the inflammatory response (12, 17). It is also possible that shedding of receptors represents a mechanism for desensitizing the cells that shed the receptors from the effects of TNF (17). On the other hand, it has been reported that at low concentrations of TNF, binding to soluble receptors can stabilize TNF and augment some of its activities (18). Thus it is possible that under some conditions the pool of TNF bound to soluble receptors could represent a reservoir for the stabilization and controlled release of TNF

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TNF RI/TNFRSF1A (DRT100)(69)

We have publications tested in 5 confirmed species: Human, Primate - Cholrocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio cynocephalus (Yellow Baboon), Primate - Papio hamadryas (Hamadyras Baboon).


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Human
(64)
Primate - Cholrocebus pygerythrus (Vervet Monkey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Primate - Papio cynocephalus (Yellow Baboon)
(2)
Primate - Papio hamadryas (Hamadyras Baboon)
(1)
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Showing Publications 1 - 10 of 69. Show All 69 Publications.
Publications using DRT100 Applications Species
M Murakoshi, T Gohda, Y Suzuki Circulating Tumor Necrosis Factor Receptors: A Potential Biomarker for the Progression of Diabetic Kidney Disease Int J Mol Sci, 2020;21(6):. 2020 [PMID: 32183005] (Human) Human
JV Baker, J Wolfson, T Peterson, M Mooberry, M Gissel, H Mystakelis, MW Henderson, K Garcia-Mye, FS Rhame, TW Schacker, KE Brummel-Zi, I Sereti, NS Key, RP Tracy Factor Xa Inhibition Reduces Coagulation Activity but Not Inflammation Among People With HIV: A Randomized Clinical Trial Open Forum Infect Dis, 2020;7(2):ofaa026. 2020 [PMID: 32055640] (Human) Human
JF Camargo, S Pallikkuth, I Moroz, Y Natori, ML Alcaide, A Rodriguez, G Guerra, GW Burke, S Pahwa Pretransplant Levels of C-Reactive Protein,�Soluble TNF Receptor-1, and�CD38+HLADR+ CD8 T Cells Predict Risk of Allograft Rejection in HIV+ Kidney Transplant Recipients Kidney Int Rep, 2019;4(12):1705-1716. 2019 [PMID: 31844807] (Human) Human
FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk, Systemic Inflammation Precedes Microalbuminuria in Diabetes Kidney Int Rep, 2019;4(10):1373-1386. 2019 [PMID: 31701047] (Human) Human
J Uwada, T Yazawa, H Nakazawa, D Mikami, SM Krug, M Fromm, K Sada, I Muramatsu, T Taniguchi Store-operated calcium entry (SOCE) contributes to phosphorylation of p38 MAPK and suppression of TNF-α signalling in the intestinal epithelial cells Cell. Signal., 2019;63(0):109358. 2019 [PMID: 31295519] (Human) Human
KP Loh, JA Tooze, BJ Nicklas, SB Kritchevsk, JD Williamson, LR Ellis, BL Powell, TS Pardee, NG Goyal, HD Klepin Inflammatory biomarkers, geriatric assessment, and treatment outcomes in acute myeloid leukemia J Geriatr Oncol, 2019;0(0):. 2019 [PMID: 30962090] (Human) Human
R Grzanka, A Damasiewic, A Kasperska- Tumor necrosis factor-alpha and Fas/Fas ligand signaling pathways in chronic spontaneous urticaria Allergy Asthma Clin Immunol, 2019;15(0):15. 2019 [PMID: 30911316] (Human) Human
H Yogasundar, A Nikhanj, BN Putko, M Boutin, S Jain-Ghai, A Khan, C Auray-Blai, ML West, GY Oudit Elevated Inflammatory Plasma Biomarkers in Patients With Fabry Disease: A Critical Link to Heart Failure With Preserved Ejection Fraction J Am Heart Assoc, 2018;7(21):e009098. 2018 [PMID: 30571380] (Human) Human
SS Foo, W Chen, Y Chan, WS Lee, SA Lee, G Cheng, K Nielsen-Sa, P Brasil, JU Jung Biomarkers and immunoprofiles associated with fetal abnormalities of ZIKV-positive pregnancies JCI Insight, 2018;3(21):. 2018 [PMID: 30385728] (Human) Human
Y Matsukiyo, H Nagai, T Matsui, Y Igarashi Host Immunological Effects of Partial Splenic Embolization in Patients with Liver Cirrhosis J Immunol Res, 2018;2018(0):1746391. 2018 [PMID: 30116748] (Human) Human
Show All 69 Publications.

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FAQs for TNF RI/TNFRSF1A (DRT100). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP DRT100
HRP DRT100
HRP DRT100

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Bioinformatics

Gene Symbol TNFRSF1A