A20/TNFAIP3 Antibody (59A426)

Images

 
Western Blot: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Human Jurkat lysate probed with A20 antibody at 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Human placenta probed with A20 antibody at 5 ug/ml, cytoplasmic staining of decidual cells is observed. Human tissue TMA was used for this ...read more
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on HeLa cells with A20/TNFAIP3 (59A426) antibody NBP1-77533F (blue) and a matched isotype control (orange). Cells were ...read more
Biological Strategies: Western Blot: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Analysis of A20 using mAb 59A426. A20 migrated as a stack of bands and LPS treatment (200 ng, ) induced a ~58 kDa putative A20 ...read more
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Intracellular flow cytometric analysis of A20 in ThP-1 cells using A20 antibody (red) and isotype control (green) at 0.5 ug/ml
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Analysis of PE conjugate of NBP1-77533. An intracellular stain was performed on Jurkat cells with A20/TNFAIP3 antibody (59A426) NBP1-77533PE (blue) and a ...read more
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on Daudi cells with A20/TNFAIP3 (59A426) antibody NBP1-77533AF488 (blue) and a matched isotype control NBP2-27287AF488 ...read more
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on SK-MEL-28 cells with A20/TNFAIP3 (59A426) antibody NBP1-77533AF647 (blue) and a matched isotype control (orange). ...read more
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on SK-MEL-28 cells with A20/TNFAIP3 (59A426) antibody NBP1-77533PE (blue) and a matched isotype control (orange). Cells ...read more
Simple Western: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Lane view shows a specific band for A20/TNFAIP3 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
59A426
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

A20/TNFAIP3 Antibody (59A426) Summary

Immunogen
Full length recombinant human A20. The epitope has been mapped to the C-terminal portion of A20 (amino acids 440-790)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNFAIP3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • Simple Western 1:100
  • Flow Cytometry 1:20-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1-2 ug/ml
  • CyTOF-ready
Application Notes
The A20 antibody (clone 59A426) has also been used in IHC-P (Metellus, 2010) and Flow Cytometry (intracellular) (Hjelmeland, 2010). Multiple A20 cleavage fragments have been described in Western Blot, see Coornaert (2008) and Hailfinger (2009) for additional details.

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. This antibody is CyTOF ready.
Control
A20/TNFAIP3 Knockout HeLa Cell Lysate
Publications
Read Publications using
NBP1-77533 in the following applications:

  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for A20/TNFAIP3 Antibody (59A426)

  • A20
  • A20TNFA1P2
  • EC 3.4.19.12
  • EC 6.3.2.-
  • MGC104522
  • MGC138687
  • OTU domain-containing protein 7C
  • OTUD7C
  • OTUD7CMGC138688
  • Putative DNA-binding protein A20
  • TNF alpha-induced protein 3
  • TNFA1P2
  • TNFAIP3
  • tumor necrosis factor alpha-induced protein 3
  • tumor necrosis factor inducible protein A20
  • tumor necrosis factor, alpha-induced protein 3
  • Zinc finger protein A20

Background

A20 (TNFAIP3) is an important regulator of proinflammatory signaling pathways, including NK-kB sginaling. A20 is upregulated through NF-kB (p50/p55) activation, and subsequently down regulates NF-kB through its dual function as a deubiquitinase and ubiquitin ligase (Evans, 2005). The A20 monoclonal antibody (clone 59A426) has been instrumental in discovering various nuances of NF-kB regulation and key to defining A20 biology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for A20/TNFAIP3 Antibody (NBP1-77533)(39)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(5)
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Filter By Species
Human
(3)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using NBP1-77533 Applications Species
Ma J, Xiao Y, Tian B et al. Genome-wide analyses of long non-coding RNA expression profiles and functional network analysis in esophageal squamous cell carcinoma Sci Rep Jun 24 2019 [PMID: 31235759] (WB, Human) WB Human
Lim MCC, Maubach G, Sokolova O et al. Pathogen-induced ubiquitin-editing enzyme A20 bifunctionally shuts off NF-kB and caspase-8-dependent apoptotic cell death. Cell Death Differ. 2017 Jun 02 [PMID: 28574503] (WB, Human) WB Human
Reihill JA, Malcomson B, Bertelsen A et al. Induction of the inflammatory regulator A20 by gibberellic acid in airway epithelial cells. Br. J. Pharmacol. 2015 May 25 [PMID: 26013851] (WB, Human) WB Human
Xuan NT, Wang X, Nishanth G et al. A20 expression in dendritic cells protects mice from LPS-induced mortality. Eur. J. Immunol. 2014 Dec 04 [PMID: 25530110] (WB, Mouse)

Details:
A20 antibody used for WB on lysates of bone marrow-derived dendritic cells (BMDCs) isolated from CD11c-Cre A20fl/fl and A20fl/fl control mice (Figure 1A).
WB Mouse
De A, Dainichi T, Rathinam CV, Ghosh S. The deubiquitinase activity of A20 is dispensable for NF-kB signaling. EMBO Rep. 2014 May 30 [PMID: 24878851] (WB, Mouse)

Details:
Primary mouse bone marrow derived macrophages, WB: Figs 1C, 5B, 5D; IP: S9. For IP, the immunoprecipitated A20 was was used in an in vitro deubiquitinase (DUB) assay (S9). Notes: TNFa and LPS treatment upregulated A20 expression (Fig 1C). The A20 mAb reco
WB Mouse
Lai Ting-Yu, Wu Shang-Duen, Tsai Mong-Hsun et al. Transcription of Tnfaip3 is regulated by NF-kappaB and p38 via C/EBPbeta in activated macrophages. PLoS One. 2013 [PMID: 24023826] (Mouse) Mouse
Svingerud T, Solstad T, Sun B et al. Atlantic salmon type I IFN subtypes show differences in antiviral activity and cell-dependent expression: evidence for high IFNb/IFNc-producing cells in fish lymphoid tissues. J Immunol. 2012 Dec 15 [PMID: 23169587]
Karube K, Nakagawa M, Tsuzuki S et al. Identification of FOXO3 and PRDM1 as tumor-suppressor gene candidates in NK-cell neoplasms by genomic and functional analyses. Blood. 2011 Sep 22 [PMID: 21690554]
Gerlach B, Cordier SM, Schmukle AC et al. Linear ubiquitination prevents inflammation and regulates immune signalling. Nature. 2011 Mar 31 [PMID: 21455173]
H0velmeyer N, Reissig S, Xuan NT et al. A20 deficiency in B cells enhances B-cell proliferation and results in the development of autoantibodies. Eur J Immunol. 2011 Mar [PMID: 21341261]
Show All 39 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for A20/TNFAIP3 Antibody (NBP1-77533) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-77533AF350
Alexa Fluor 405 NBP1-77533AF405
Alexa Fluor 488 NBP1-77533AF488
Alexa Fluor 532 NBP1-77533AF532
Alexa Fluor 594 NBP1-77533AF594
Alexa Fluor 647 NBP1-77533AF647
Alexa Fluor 700 NBP1-77533AF700
Alexa Fluor 750 NBP1-77533AF750
Allophycocyanin NBP1-77533APC
Biotin NBP1-77533B
DyLight 350 NBP1-77533UV
DyLight 405 NBP1-77533V
DyLight 488 NBP1-77533G
DyLight 550 NBP1-77533R
DyLight 594 NBP1-77533DL594
DyLight 650 NBP1-77533C
DyLight 680 NBP1-77533FR
DyLight 755 NBP1-77533IR
FITC NBP1-77533F
HRP NBP1-77533H
Janelia Fluor 549 NBP1-77533JF549
Janelia Fluor 646 NBP1-77533JF646
PE NBP1-77533PE
PerCP NBP1-77533PCP

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Bioinformatics

Gene Symbol TNFAIP3
Uniprot