Western Blot: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Human Jurkat lysate probed with A20 antibody at 4 ug/ml.
Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Formalin-fixed, paraffin-embedded human placenta probed with A20 antibody at 5 ug/ml, cytoplasmic staining of decidual cells is observed. Human ...read more
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Intracellular flow cytometric analysis of A20 in ThP-1 cells using A20 antibody (red) and isotype control (green) at 0.5 ug/ml
Western Blot: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Analysis of A20 using mAb 59A426. A20 migrated as a stack of bands and LPS treatment (200 ng, ) induced a ~58 kDa putative A20 cleavage band* not seen in the ...read more
Simple Western: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Simple Western lane view shows a specific band for A20/TNFAIP3 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using ...read more
The A20 antibody (clone 59A426) has also been used in Immunohistochemistry-Paraffin (Metellus, 2010) and Flow Cytometry (intracellular) (Hjelmeland, 2010). Multiple A20 cleavage fragments have been described in Western Blot, see Coornaert (2008) and Hailfinger (2009) for additional details. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publications using NBP1-77533 in the following applications:
A20 (TNFAIP3) is an important regulator of proinflammatory signaling pathways, including NK-kB sginaling. A20 is upregulated through NF-kB (p50/p55) activation, and subsequently down regulates NF-kB through its dual function as a deubiquitinase and ubiquitin ligase (Evans, 2005). The A20 monoclonal antibody (clone 59A426) has been instrumental in discovering various nuances of NF-kB regulation and key to defining A20 biology.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.