A20/TNFAIP3 Antibody (59A426)


Western Blot: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Human Jurkat lysate probed with A20 antibody at 4 ug/ml.
Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Human placenta probed with A20 antibody at 5 ug/ml, cytoplasmic staining of decidual cells is observed. Human tissue TMA was used for this ...read more
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on HeLa cells with A20/TNFAIP3 (59A426) antibody NBP1-77533F (blue) and a matched isotype control (orange). Cells were ...read more
Biological Strategies: Western Blot: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Analysis of A20 using mAb 59A426. A20 migrated as a stack of bands and LPS treatment (200 ng, ) induced a ~58 kDa putative A20 ...read more
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Intracellular flow cytometric analysis of A20 in ThP-1 cells using A20 antibody (red) and isotype control (green) at 0.5 ug/ml
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Analysis of PE conjugate of NBP1-77533. An intracellular stain was performed on Jurkat cells with A20/TNFAIP3 antibody (59A426) NBP1-77533PE (blue) and a ...read more
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on Daudi cells with A20/TNFAIP3 (59A426) antibody NBP1-77533AF488 (blue) and a matched isotype control NBP2-27287AF488 ...read more
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on SK-MEL-28 cells with A20/TNFAIP3 (59A426) antibody NBP1-77533AF647 (blue) and a matched isotype control (orange). ...read more
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on SK-MEL-28 cells with A20/TNFAIP3 (59A426) antibody NBP1-77533PE (blue) and a matched isotype control (orange). Cells ...read more
Simple Western: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Lane view shows a specific band for A20/TNFAIP3 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC-P, IP, CyTOF-ready
1.0 mg/ml
Validated by:

Biological Strategies


Order Details

A20/TNFAIP3 Antibody (59A426) Summary

Full length recombinant human A20. The epitope has been mapped to the C-terminal portion of A20 (amino acids 440-790)
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 2-4 ug/ml
  • Simple Western 1:100
  • Flow Cytometry 1:20-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1-2 ug/ml
  • CyTOF-ready
Application Notes
The A20 antibody (clone 59A426) has also been used in IHC-P (Metellus, 2010) and Flow Cytometry (intracellular) (Hjelmeland, 2010). Multiple A20 cleavage fragments have been described in Western Blot, see Coornaert (2008) and Hailfinger (2009) for additional details.

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. This antibody is CyTOF ready.
Read Publications using
NBP1-77533 in the following applications:

  • WB
    4 publications

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for A20/TNFAIP3 Antibody (59A426)

  • A20
  • A20TNFA1P2
  • EC
  • EC 6.3.2.-
  • MGC104522
  • MGC138687
  • OTU domain-containing protein 7C
  • OTUD7C
  • OTUD7CMGC138688
  • Putative DNA-binding protein A20
  • TNF alpha-induced protein 3
  • TNFA1P2
  • tumor necrosis factor alpha-induced protein 3
  • tumor necrosis factor inducible protein A20
  • tumor necrosis factor, alpha-induced protein 3
  • Zinc finger protein A20


A20 (TNFAIP3) is an important regulator of proinflammatory signaling pathways, including NK-kB sginaling. A20 is upregulated through NF-kB (p50/p55) activation, and subsequently down regulates NF-kB through its dual function as a deubiquitinase and ubiquitin ligase (Evans, 2005). The A20 monoclonal antibody (clone 59A426) has been instrumental in discovering various nuances of NF-kB regulation and key to defining A20 biology.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P
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Applications: WB, Simple Western, IP
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, ELISA(Cap), Flow-CS, Flow-IC
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Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
Species: Mu
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC-P, IP, CyTOF-ready
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Publications for A20/TNFAIP3 Antibody (NBP1-77533)(38)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.

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Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using NBP1-77533 Applications Species
Lim MCC, Maubach G, Sokolova O et al. Pathogen-induced ubiquitin-editing enzyme A20 bifunctionally shuts off NF-kB and caspase-8-dependent apoptotic cell death. Cell Death Differ. 2017 Jun 02 [PMID: 28574503] (WB, Human) WB Human
Reihill JA, Malcomson B, Bertelsen A et al. Induction of the inflammatory regulator A20 by gibberellic acid in airway epithelial cells. Br. J. Pharmacol. 2015 May 25 [PMID: 26013851] (WB, Human) WB Human
Xuan NT, Wang X, Nishanth G et al. A20 expression in dendritic cells protects mice from LPS-induced mortality. Eur. J. Immunol. 2014 Dec 04 [PMID: 25530110] (WB, Mouse)

A20 antibody used for WB on lysates of bone marrow-derived dendritic cells (BMDCs) isolated from CD11c-Cre A20fl/fl and A20fl/fl control mice (Figure 1A).
WB Mouse
De A, Dainichi T, Rathinam CV, Ghosh S. The deubiquitinase activity of A20 is dispensable for NF-kB signaling. EMBO Rep. 2014 May 30 [PMID: 24878851] (WB, Mouse)

Primary mouse bone marrow derived macrophages, WB: Figs 1C, 5B, 5D; IP: S9. For IP, the immunoprecipitated A20 was was used in an in vitro deubiquitinase (DUB) assay (S9). Notes: TNFa and LPS treatment upregulated A20 expression (Fig 1C). The A20 mAb reco
WB Mouse
Lai Ting-Yu, Wu Shang-Duen, Tsai Mong-Hsun et al. Transcription of Tnfaip3 is regulated by NF-kappaB and p38 via C/EBPbeta in activated macrophages. PLoS One. 2013 [PMID: 24023826] (Mouse) Mouse
Svingerud T, Solstad T, Sun B et al. Atlantic salmon type I IFN subtypes show differences in antiviral activity and cell-dependent expression: evidence for high IFNb/IFNc-producing cells in fish lymphoid tissues. J Immunol. 2012 Dec 15 [PMID: 23169587]
Karube K, Nakagawa M, Tsuzuki S et al. Identification of FOXO3 and PRDM1 as tumor-suppressor gene candidates in NK-cell neoplasms by genomic and functional analyses. Blood. 2011 Sep 22 [PMID: 21690554]
Gerlach B, Cordier SM, Schmukle AC et al. Linear ubiquitination prevents inflammation and regulates immune signalling. Nature. 2011 Mar 31 [PMID: 21455173]
H0velmeyer N, Reissig S, Xuan NT et al. A20 deficiency in B cells enhances B-cell proliferation and results in the development of autoantibodies. Eur J Immunol. 2011 Mar [PMID: 21341261]
Adrianto I, Wen F, Templeton A et al. Association of a functional variant downstream of TNFAIP3 with systemic lupus erythematosus. Nat Genet. 2011 Mar [PMID: 21336280]
Show All 38 Publications.

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WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-77533AF405
Alexa Fluor 488 Labeled NBP1-77533AF488
Alexa Fluor 647 Labeled NBP1-77533AF647
Alexa Fluor 700 Labeled NBP1-77533AF700
Allophycocyanin Labeled NBP1-77533APC
Biotin Labeled NBP1-77533B
DyLight 350 Labeled NBP1-77533UV
DyLight 405 Labeled NBP1-77533V
DyLight 488 Labeled NBP1-77533G
DyLight 550 Labeled NBP1-77533R
DyLight 650 Labeled NBP1-77533C
DyLight 680 Labeled NBP1-77533FR
DyLight 755 Labeled NBP1-77533IR
HRP Labeled NBP1-77533H
PE Labeled NBP1-77533PE
PerCP Labeled NBP1-77533PCP

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Gene Symbol TNFAIP3