A20/TNFAIP3 Antibody (59A426)

Images

 
Western Blot: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Human Jurkat lysate probed with A20 antibody at 4 ug/ml.
Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Formalin-fixed, paraffin-embedded human placenta probed with A20 antibody at 5 ug/ml, cytoplasmic staining of decidual cells is observed. Human ...read more
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on Daudi cells with A20/TNFAIP3 (59A426) antibody NBP1-77533AF488 (blue) and a matched isotype control NBP2-27287AF488 ...read more
Western Blot: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Analysis of A20 using mAb 59A426. A20 migrated as a stack of bands and LPS treatment (200 ng, ) induced a ~58 kDa putative A20 cleavage band* not seen in the ...read more
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Intracellular flow cytometric analysis of A20 in ThP-1 cells using A20 antibody (red) and isotype control (green) at 0.5 ug/ml
Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Analysis of PE conjugate of NBP1-77533. An intracellular stain was performed on Jurkat cells with A20/TNFAIP3 antibody (59A426) NBP1-77533PE (blue) and a ...read more
Simple Western: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Simple Western lane view shows a specific band for A20/TNFAIP3 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC-P, IP, CyTOF-ready
Clone
59A426
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

A20/TNFAIP3 Antibody (59A426) Summary

Immunogen
Full length recombinant human A20. The epitope has been mapped to the C-terminal portion of A20 (amino acids 440-790)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNFAIP3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • Simple Western 1:100
  • Flow Cytometry 1:20-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1-2 ug/ml
  • CyTOF-ready
Application Notes
The A20 antibody (clone 59A426) has also been used in Immunohistochemistry-Paraffin (Metellus, 2010) and Flow Cytometry (intracellular) (Hjelmeland, 2010). Multiple A20 cleavage fragments have been described in Western Blot, see Coornaert (2008) and Hailfinger (2009) for additional details.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Publications
Read Publications using
NBP1-77533 in the following applications:

  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for A20/TNFAIP3 Antibody (59A426)

  • A20
  • A20TNFA1P2
  • EC 3.4.19.12
  • EC 6.3.2.-
  • MGC104522
  • MGC138687
  • OTU domain-containing protein 7C
  • OTUD7C
  • OTUD7CMGC138688
  • Putative DNA-binding protein A20
  • TNF alpha-induced protein 3
  • TNFA1P2
  • TNFAIP3
  • tumor necrosis factor alpha-induced protein 3
  • tumor necrosis factor inducible protein A20
  • tumor necrosis factor, alpha-induced protein 3
  • Zinc finger protein A20

Background

A20 (TNFAIP3) is an important regulator of proinflammatory signaling pathways, including NK-kB sginaling. A20 is upregulated through NF-kB (p50/p55) activation, and subsequently down regulates NF-kB through its dual function as a deubiquitinase and ubiquitin ligase (Evans, 2005). The A20 monoclonal antibody (clone 59A426) has been instrumental in discovering various nuances of NF-kB regulation and key to defining A20 biology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
NB100-56507
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
NB100-56715
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-77077
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
DDX0130P-100
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-P
NBP1-89306
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB100-56170
Species: Hu, Bv, Ca
Applications: WB, IHC, IHC-P, IP
NB120-13550
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56104
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NB100-56566
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, Flow-CS, Flow-IC
NB100-56098
Species: Hu, Mu, Ca
Applications: WB, IHC, IHC-P, IP
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
796-IC
Species: Mu
NBP1-97507
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Supplier Logo

Publications for A20/TNFAIP3 Antibody (NBP1-77533)(38)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(4)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using NBP1-77533 Applications Species
Lim MCC, Maubach G, Sokolova O et al. Pathogen-induced ubiquitin-editing enzyme A20 bifunctionally shuts off NF-kB and caspase-8-dependent apoptotic cell death. Cell Death Differ. 2017 Jun 02 [PMID: 28574503] (WB, Human) WB Human
Reihill JA, Malcomson B, Bertelsen A et al. Induction of the inflammatory regulator A20 by gibberellic acid in airway epithelial cells. Br. J. Pharmacol. 2015 May 25 [PMID: 26013851] (WB, Human) WB Human
Xuan NT, Wang X, Nishanth G et al. A20 expression in dendritic cells protects mice from LPS-induced mortality. Eur. J. Immunol. 2014 Dec 04 [PMID: 25530110] (WB, Mouse)

Details:
A20 antibody used for WB on lysates of bone marrow-derived dendritic cells (BMDCs) isolated from CD11c-Cre A20fl/fl and A20fl/fl control mice (Figure 1A).
WB Mouse
De A, Dainichi T, Rathinam CV, Ghosh S. The deubiquitinase activity of A20 is dispensable for NF-kB signaling. EMBO Rep. 2014 May 30 [PMID: 24878851] (WB, Mouse)

Details:
Primary mouse bone marrow derived macrophages, WB: Figs 1C, 5B, 5D; IP: S9. For IP, the immunoprecipitated A20 was was used in an in vitro deubiquitinase (DUB) assay (S9). Notes: TNFa and LPS treatment upregulated A20 expression (Fig 1C). The A20 mAb reco
WB Mouse
Lai Ting-Yu, Wu Shang-Duen, Tsai Mong-Hsun et al. Transcription of Tnfaip3 is regulated by NF-kappaB and p38 via C/EBPbeta in activated macrophages. PLoS One. 2013 [PMID: 24023826] (Mouse) Mouse
Svingerud T, Solstad T, Sun B et al. Atlantic salmon type I IFN subtypes show differences in antiviral activity and cell-dependent expression: evidence for high IFNb/IFNc-producing cells in fish lymphoid tissues. J Immunol. 2012 Dec 15 [PMID: 23169587]
Karube K, Nakagawa M, Tsuzuki S et al. Identification of FOXO3 and PRDM1 as tumor-suppressor gene candidates in NK-cell neoplasms by genomic and functional analyses. Blood. 2011 Sep 22 [PMID: 21690554]
Gerlach B, Cordier SM, Schmukle AC et al. Linear ubiquitination prevents inflammation and regulates immune signalling. Nature. 2011 Mar 31 [PMID: 21455173]
H0velmeyer N, Reissig S, Xuan NT et al. A20 deficiency in B cells enhances B-cell proliferation and results in the development of autoantibodies. Eur J Immunol. 2011 Mar [PMID: 21341261]
Adrianto I, Wen F, Templeton A et al. Association of a functional variant downstream of TNFAIP3 with systemic lupus erythematosus. Nat Genet. 2011 Mar [PMID: 21336280]
Show All 38 Publications.

Reviews for A20/TNFAIP3 Antibody (NBP1-77533) (0)

There are no reviews for A20/TNFAIP3 Antibody (NBP1-77533). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for A20/TNFAIP3 Antibody (NBP1-77533) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-77533AF405
Alexa Fluor 488 Labeled NBP1-77533AF488
Alexa Fluor 647 Labeled NBP1-77533AF647
Alexa Fluor 700 Labeled NBP1-77533AF700
Allophycocyanin Labeled NBP1-77533APC
Biotin Labeled NBP1-77533B
DyLight 350 Labeled NBP1-77533UV
DyLight 405 Labeled NBP1-77533V
DyLight 488 Labeled NBP1-77533G
DyLight 550 Labeled NBP1-77533R
DyLight 650 Labeled NBP1-77533C
DyLight 680 Labeled NBP1-77533FR
DyLight 755 Labeled NBP1-77533IR
HRP Labeled NBP1-77533H
PE Labeled NBP1-77533PE
PerCP Labeled NBP1-77533PCP

Additional A20/TNFAIP3 Products

Bioinformatics Tool for A20/TNFAIP3 Antibody (NBP1-77533)

Discover related pathways, diseases and genes to A20/TNFAIP3 Antibody (NBP1-77533). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for A20/TNFAIP3 Antibody (NBP1-77533)

Discover more about diseases related to A20/TNFAIP3 Antibody (NBP1-77533).
 

Pathways for A20/TNFAIP3 Antibody (NBP1-77533)

View related products by pathway.

PTMs for A20/TNFAIP3 Antibody (NBP1-77533)

Learn more about PTMs related to A20/TNFAIP3 Antibody (NBP1-77533).
 

Research Areas for A20/TNFAIP3 Antibody (NBP1-77533)

Find related products by research area.

Blogs on A20/TNFAIP3

There are no specific blogs for A20/TNFAIP3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our A20/TNFAIP3 Antibody (59A426) and receive a gift card or discount.

Bioinformatics

Gene Symbol TNFAIP3
Entrez
Uniprot