TLR4 Antibody (MTS510) [FITC]


Flow Cytometry: TLR4 Antibody (MTS510) [FITC] [NBP2-24450] - Cell surface analysis of TLR4 in RAW cells using 0.5 ug of this antibody. Black histogram represents cells without antibody; green represents isotype control; more
Flow Cytometry: TLR4 Antibody (MTS510) [FITC] [NBP2-24450] - Cell surface analysis of TLR4 in mouse peritoneal cells using 1 ug of this antibody. Shaded histogram represents cells without antibody; green represents more
Flow Cytometry: TLR4 Antibody (MTS510) [FITC] [NBP2-24450] - Cell surface analysis of TLR4 in mouse splenocytes using 2 ug of this antibody. Cells were stimulated with 1 ug/ml anti-CD3 for 72 hours. Shaded histogram more

Product Details

Reactivity MuSpecies Glossary
Applications Flow
Please see the vial label for concentration. If unlisted please contact technical services.

TLR4 Antibody (MTS510) [FITC] Summary

This antibody was developed by immunizing rats with the Ba/F3 cell line expressing mouse TLR4 and MD-2 (Akashi et al, 2000).
In FA, the antibody blocks activation of monocytes with LPS (Akashi et al. 2000). This antibody preferentially recognizes TLR4-MD-2 complex than of TLR4 alone. The optimal condition has to be determined for individual experiments.
IgG2a Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
FITC (A=495, E=519)
50mM Sodium Borate
0.05% Sodium Azide
Protein G purified


  • Flow Cytometry 1ul/1 million cells
Read Publications using
NBP2-24450 in the following applications:

Alternate Names for TLR4 Antibody (MTS510) [FITC]

  • ARMD10
  • CD284 antigen
  • CD284
  • hTollhomolog of Drosophila toll
  • TOLL
  • toll-like receptor 4


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TLR4 Antibody (NBP2-24450)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow, Flow-CS, ICC/IF, WB.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-24450 Applications Species
Tsai CC, Lin CR, Tsai HY et al. The immunologically active oligosaccharides isolated from wheatgrass modulate monocytes via Toll-like receptor-2 signaling. J Biol Chem. 2013 Jun 14 [PMID:23629653]
Kim MY, Shu Y, Carsillo T et al. hsp70 and a novel axis of type I interferon-dependent antiviral immunity in the measles virus-infected brain. J Virol. 2013 Jan [PMID:23135720]
Ma CY, Shi GY, Shi CS et al. Monocytic thrombomodulin triggers LPS- and gram-negative bacteria-induced inflammatory response. J Immunol. 2012 Jun 15 [PMID:22573811] (ICC/IF, Mouse) ICC/IF Mouse
Bruscia EM, Zhang PX, Satoh A et al. Abnormal trafficking and degradation of TLR4 underlie the elevated inflammatory response in cystic fibrosis. J Immunol. 2011 Jun 15 [PMID:21593379] (WB, ICC/IF, Flow-CS, Human, Mouse) WB, ICC/IF, Flow-CS Human, Mouse
Park HJ, Hong JH, Kwon HJ et al. TLR4-mediated activation of mouse macrophages by Korean mistletoe lectin-C (KML-C). Biochem Biophys Res Commun. 2010 Jun 4 [PMID:20450885] (WB, Mouse) WB Mouse
Tsukamoto H, Fukudome K, Takao S et al. Lipopolysaccharide-binding protein-mediated Toll-like receptor 4 dimerization enables rapid signal transduction against lipopolysaccharide stimulation on membrane-associated CD14-expressing cells. Int Immunol. 2010 Apr [PMID:20133493] (WB) WB
Macedo GC, Magnani DM, Carvalho NB et al. Central role of MyD88-dependent dendritic cell maturation and proinflammatory cytokine production to control Brucella abortus infection. J Immunol. 2008 Jan 15 [PMID:18178848] (Flow, Mouse) Flow Mouse
Corbucci C, Cenci E, Skrzypek F et al. Immune response to Candida albicans is preserved despite defect in O-mannosylation of secretory proteins. Med Mycol. 2007 Dec [PMID:17885949] (Flow-CS) Flow-CS
El Shikh ME, El Sayed RM, Wu Y et al. TLR4 on follicular dendritic cells: an activation pathway that promotes accessory activity. J Immunol. 2007 Oct 1 [PMID:17878340] (Flow-CS) Flow-CS
Konno K, Wakabayashi Y, Akashi-Takamura S et al. A molecule that is associated with Toll-like receptor 4 and regulates its cell surface expression. Biochem Biophys Res Commun. 2006 Jan 27 [PMID:16338228]
Show All 11 Publications.

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Secondary Antibodies


Isotype Controls

Other Available Formats

DyLight 350 Labeled NBP2-24865UV
DyLight 405 Labeled NBP2-24865V
DyLight 405LS Labeled NBP2-24865V3
DyLight 488 Labeled NBP2-24865G
DyLight 550 Labeled NBP2-24865R
DyLight 650 Labeled NBP2-24865C
DyLight 680 Labeled NBP2-24865FR
DyLight 755 Labeled NBP2-24865IR
HRP Labeled NBP2-24865H
PE Labeled NBP2-24741
PerCP Labeled NBP2-24865PCP

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Blogs on TLR4.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR4: Playing Roles in Apoptosis and Autoimmunity
TLR4 (Toll-like receptor 4) is a member of the Toll-like receptor (TLR) family that plays a key role in pathogen recognition and activation of innate immunity. Scientists have found that TLRs are highly conserved from Drosophila to humans, with a high...  Read full blog post.

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Gene Symbol TLR4

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