Flow Cytometry: TLR4 Antibody (MTS510) [FITC] [NBP2-24450] - Cell surface analysis of TLR4 in RAW cells using 0.5 ug of this antibody. Black histogram represents cells without antibody; green represents isotype control; ...read more
Flow Cytometry: TLR4 Antibody (MTS510) [FITC] [NBP2-24450] - Cell surface analysis of TLR4 in mouse peritoneal cells using 1 ug of this antibody. Shaded histogram represents cells without antibody; green represents ...read more
Flow Cytometry: TLR4 Antibody (MTS510) [FITC] [NBP2-24450] - Cell surface analysis of TLR4 in mouse splenocytes using 2 ug of this antibody. Cells were stimulated with 1 ug/ml anti-CD3 for 72 hours. Shaded histogram ...read more
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This antibody was developed by immunizing rats with the Ba/F3 cell line expressing mouse TLR4 and MD-2 (Akashi et al, 2000).
In FA, the antibody blocks activation of monocytes with LPS (Akashi et al. 2000). This antibody preferentially recognizes TLR4-MD-2 complex than of TLR4 alone. The optimal condition has to be determined for individual experiments.
Protein G purified
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