Flow (Cell Surface): TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide/BSA FREE version of NBP2-24865. Cell surface analysis of TLR4 on mouse RAW cells using 0.5 ug/10^6 cells of TLR4 anitbody, anti-rat APC ...read more
Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide Free version of NB100-56560. Cell surface TLR4 in mouse peritoneal cells using this antibody. The green histogram represents the isotype ...read more
Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide Free version of NB100-56560. Cell surface TLR4 FITC in RAW cells using 0.5 ug of this antibody. Black histogram represents cells without ...read more
Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis of PE conjugate of NB100-56560. Cell surface TLR4 in mouse peritoneal cells using 0.5 ug of this antibody. Shaded histogram represents cells without ...read more
This antibody was developed by immunizing rats with the Ba/F3 cell line expressing mouse TLR4 and MD-2 (Akashi et al, 2000).
In FA, the antibody blocks activation of monocytes with LPS (Akashi et al. 2000). This antibody preferentially recognizes TLR4-MD-2 complex than of TLR4 alone. The optimal condition has to be determined for individual experiments.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 0.05% BSA
0.05% Sodium Azide
Protein G purified
Alternate Names for TLR4 Antibody (MTS510)
hTollhomolog of Drosophila toll
toll-like receptor 4
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The mouse TLR4 cDNA codes for a protein consisting of 835 amino acids with approximate molecular weight of 90 kDa.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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