TLR4 Antibody (MTS510)


Flow (Cell Surface): TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide/BSA FREE version of NBP2-24865. Cell surface analysis of TLR4 on mouse RAW cells using 0.5 ug/10^6 cells of TLR4 anitbody, anti-rat APC more
Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide Free version of NB100-56560. Cell surface TLR4 in mouse peritoneal cells. Green: isotype control. Red: anti-TLR4 antibody.
Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide Free version of NB100-56560. Cell surface TLR4 FITC in RAW cells using 0.5 ug of this antibody. Black: cells without antibody. Green: isotype more
Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the FITC conjugate of NBP2-24865. Staining of TLR4 in mouse peritoneal cells using 1 ug of this antibody. Shaded histogram: Cells without antibody; more
Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis of PE conjugate of NB100-56560. Cell surface TLR4 in mouse peritoneal cells using 0.5 ug of this antibody. Shaded histogram: Cells without antibody. Green: more
Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the PE conjugate of NBP2-24865. Staining of cell surface TLR4 using this antibody at 0.625 ug/10^6 Raw cells (pretreated with anti-CD16/32). Shaded more

Product Details

Reactivity MuSpecies Glossary
Applications ELISA, Flow, Flow-CS, ICC/IF, IP
0.5 mg/ml

Order Details

TLR4 Antibody (MTS510) Summary

This antibody was developed by immunizing rats with the Ba/F3 cell line expressing mouse TLR4 and MD-2 (Akashi et al, 2000).
In FA, the antibody blocks activation of monocytes with LPS (Akashi et al. 2000). This antibody preferentially recognizes TLR4-MD-2 complex than of TLR4 alone.
IgG2a Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 1 - 2 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 1:10 - 1:500
  • Flow (Cell Surface) 1 - 2 ug/10^6 cells
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 22573811). Use in ELISA reported in scientific literature (PMID: 23629653).
Reviewed Applications
Read 1 Review rated 4
NB100-56560 in the following applications:

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NB100-56560 in the following applications:

Reactivity Notes

Mouse. Not yet tested in other species.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 0.05% BSA
0.05% Sodium Azide
0.5 mg/ml
Protein G purified

Alternate Names for TLR4 Antibody (MTS510)

  • ARMD10
  • CD284 antigen
  • CD284
  • hTollhomolog of Drosophila toll
  • mts510 tlr4
  • mts510
  • TLR4
  • TOLL
  • toll-like receptor 4


The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The mouse TLR4 cDNA codes for a protein consisting of 835 amino acids with approximate molecular weight of 90 kDa.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TLR4 Antibody (NB100-56560)(14)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Flow, Flow-CS, ICC/IF, IP.

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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB100-56560 Applications Species
Tsai CC, Lin CR, Tsai HY et al. The immunologically active oligosaccharides isolated from wheatgrass modulate monocytes via Toll-like receptor-2 signaling. J Biol Chem. 2013 Jun 14 [PMID: 23629653]

Antibodies cited (THP-1 cells): 1. TLR2, clone TL2.1 (IMG-416E): Neutralization assays (Fig 7), pre-treatment with the TLR2 mAb inhibited maltoheptaose-stimulated cytokine expression. Inhibition was TLR2 mAb dose dependent; WB (Fig 7), TLR2 was detected a
Schaefer L, Babelova A, Kiss E et al. The matrix component biglycan is proinflammatory and signals through Toll-like receptors 4 and 2 in macrophages J Clin Invest 2005 Aug [PMID: 16025156]

This reference used the Azide Free version of NB100-56560.
Jung DY, Lee H, Jung BY et al. TLR4, but not TLR2, signals autoregulatory apoptosis of cultured microglia: a critical role of IFN-beta as a decision maker J Immunol 2005 May 15 [PMID: 15879150] (Flow-CS)

This reference used the Azide Free version of NB100-56560.
Jung DY, Lee H, Jung BY et al. TLR4, but not TLR2, signals autoregulatory apoptosis of cultured microglia: a critical role of IFN-beta as a decision maker. J Immunol. (Flow-CS)

This citation used the Azide Free form of this antibody.
Kim MY, Shu Y, Carsillo T et al. hsp70 and a novel axis of type I interferon-dependent antiviral immunity in the measles virus-infected brain. J Virol. 2013 Jan [PMID: 23135720]
Ma CY, Shi GY, Shi CS et al. Monocytic thrombomodulin triggers LPS- and gram-negative bacteria-induced inflammatory response. J Immunol. 2012 Jun 15 [PMID: 22573811] (ICC/IF, Mouse)

TLR4 (IMG-428A). IF: mouse peritoneal macrophages (Fig 5D).
ICC/IF Mouse
Bruscia EM, Zhang PX, Satoh A et al. Abnormal trafficking and degradation of TLR4 underlie the elevated inflammatory response in cystic fibrosis. J Immunol. 2011 Jun 15 [PMID: 21593379]

1. TLR4/CD284 (IMG-428C) & TLR2/CD282 (IMG-6320C) : WB, IF/ICC, Flow (cell surface): Human macrophages with cystic fibrosis (CF) and wild type (WT) mouse macrophages, Fig 1 & S4. 2. TLR4/CD284 WB: CF and wild type macrophages treated with protein synthesi
Park HJ, Hong JH, Kwon HJ et al. TLR4-mediated activation of mouse macrophages by Korean mistletoe lectin-C (KML-C). Biochem Biophys Res Commun. 2010 Jun 4 [PMID: 20450885]

The following antibodies were used: TLR4/CD284 (IMG-428E), TLR4/CD284 (IMG-577), and TLR4 (IMG-6307A). IMG-577 & IMG-6307A were used in Fig 1 (WB): Peritoneal primary mouse (BALB/C) macrophages stimulated with LPS and then treated with KML-C (Korean mistl
Tsukamoto H, K Fukudome, S Takao, N Tsuneyoshi, M Kimoto. Lipopolysaccharide-binding protein-mediated Toll-like receptor 4 dimerization enables rapid signal transduction against lipopolysaccharide stimulation on membrane-associated CD-14 expressing cells. Int Immuno 22: 271-280. 2010 [PMID: 20133493] (Flow-CS)

Products cited: 1. TLR4 (IMG-578): WB, TLR4 stably transfected Ba/F3 cells. Note: These cells were immunoprecipitated with a TLR4 mAb (clone UT41) then western blotted with the IMG-578A TLR4 pAb Fig 3B. 3. TLR4 (IMG-428A): Flow (cell surface), RAW264 and
Macedo GC, Magnani DM, Carvalho NB et al. Central role of MyD88-dependent dendritic cell maturation and proinflammatory cytokine production to control Brucella abortus infection. J Immunol. 2008 Jan 15 [PMID: 18178848] (Flow, Mouse)

IMG-428E: FA (mouse bone marrow derived and splenic dentritic cells), Fig. 7C,D.
Flow Mouse
Show All 14 Publications.

Review for TLR4 Antibody (NB100-56560) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-56560:
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Images Ratings Applications Species Date Details
Block/Neutralize TLR4 NB100-56560
reviewed by:
Jim Hsiao
B/N Mouse 06/07/2017


Sample TestedPrimary Mouse Lung Endothelial Cells


CommentsTLR4 (MTS510): 20 ug/ml

Product General Protocols

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ICC/IF Video Protocol

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Secondary Antibodies


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Blogs on TLR4. Showing 1-10 of 11 blog posts - Show all blog posts.

PAMPs and DAMPs: What is the same and what is different about these molecules?
By Victoria OsinskiWhat are PAMPs and DAMPsInflammation results from stimuli signaling damage or infection. The inflammatory response can be beneficial or harmful depending on the type and duration of stimuli. The s...  Read full blog post.

How to identify B cell subsets using flow cytometry
By Victoria OsinskiUsing flow cytometry to identify B cell subsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a...  Read full blog post.

Lipopolysaccharide from gut microbiome localizes in human atherosclerotic plaques and promotes TLR4-mediated oxidative stress
By Jamshed Arslan, Pharm. D., PhD. Atherosclerosis is a chronic inflammatory condition in which plaques of fats and other substances slowly buildup on the inner walls of arteries to restrict blood flow. In atheroscle...  Read full blog post.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.

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Recent Reviews


Jim Hsiao
Application: B/N
Species: Mouse


Gene Symbol TLR4