TLR4 Antibody (MTS510)

Images

 
Flow (Cell Surface): TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide/BSA FREE version of NBP2-24865. Cell surface analysis of TLR4 on mouse RAW cells using 0.5 ug/10^6 cells of TLR4 anitbody, anti-rat APC ...read more
Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide Free version of NB100-56560. Cell surface TLR4 in mouse peritoneal cells using this antibody. The green histogram represents the isotype ...read more
Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide Free version of NB100-56560. Cell surface TLR4 FITC in RAW cells using 0.5 ug of this antibody. Black histogram represents cells without ...read more
Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis of PE conjugate of NB100-56560. Cell surface TLR4 in mouse peritoneal cells using 0.5 ug of this antibody. Shaded histogram represents cells without ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, Flow-CS, IP
Clone
MTS510
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

TLR4 Antibody (MTS510) Summary

Immunogen
This antibody was developed by immunizing rats with the Ba/F3 cell line expressing mouse TLR4 and MD-2 (Akashi et al, 2000).
Specificity
In FA, the antibody blocks activation of monocytes with LPS (Akashi et al. 2000). This antibody preferentially recognizes TLR4-MD-2 complex than of TLR4 alone. The optimal condition has to be determined for individual experiments.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
TLR4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/10^6 cells
  • Immunoprecipitation 1:10-1:500
  • Flow (Cell Surface) 1-2 ug/10^6 cells
Application Notes
Immunoprecipitation: Please see Akashi et al (2000) for details
Agonist
Antagonist
Reviewed Applications
Read 1 Review rated 4
using
NB100-56560 in the following applications:

Publications
Read Publications using
NB100-56560 in the following applications:

Reactivity Notes

Mouse. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for TLR4 Antibody (MTS510)

  • ARMD10
  • CD284 antigen
  • CD284
  • hTollhomolog of Drosophila toll
  • mts510 tlr4
  • mts510
  • TLR4
  • TOLL
  • toll-like receptor 4

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The mouse TLR4 cDNA codes for a protein consisting of 835 amino acids with approximate molecular weight of 90 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TLR4 Antibody (NB100-56560)(13)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Flow, Flow-CS, ICC/IF, IP.


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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-56560 Applications Species
Tsai CC, Lin CR, Tsai HY et al. The immunologically active oligosaccharides isolated from wheatgrass modulate monocytes via Toll-like receptor-2 signaling. J Biol Chem. 2013 Jun 14 [PMID: 23629653]

Details:
Antibodies cited (THP-1 cells): 1. TLR2, clone TL2.1 (IMG-416E): Neutralization assays (Fig 7), pre-treatment with the TLR2 mAb inhibited maltoheptaose-stimulated cytokine expression. Inhibition was TLR2 mAb dose dependent; WB (Fig 7), TLR2 was detected a
Kim MY, Shu Y, Carsillo T et al. hsp70 and a novel axis of type I interferon-dependent antiviral immunity in the measles virus-infected brain. J Virol. 2013 Jan [PMID: 23135720]
Ma CY, Shi GY, Shi CS et al. Monocytic thrombomodulin triggers LPS- and gram-negative bacteria-induced inflammatory response. J Immunol. 2012 Jun 15 [PMID: 22573811] (ICC/IF, Mouse)

Details:
TLR4 (IMG-428A). IF: mouse peritoneal macrophages (Fig 5D).
ICC/IF Mouse
Bruscia EM, Zhang PX, Satoh A et al. Abnormal trafficking and degradation of TLR4 underlie the elevated inflammatory response in cystic fibrosis. J Immunol. 2011 Jun 15 [PMID: 21593379]

Details:
1. TLR4/CD284 (IMG-428C) & TLR2/CD282 (IMG-6320C) : WB, IF/ICC, Flow (cell surface): Human macrophages with cystic fibrosis (CF) and wild type (WT) mouse macrophages, Fig 1 & S4. 2. TLR4/CD284 WB: CF and wild type macrophages treated with protein synthesi
Park HJ, Hong JH, Kwon HJ et al. TLR4-mediated activation of mouse macrophages by Korean mistletoe lectin-C (KML-C). Biochem Biophys Res Commun. 2010 Jun 4 [PMID: 20450885]

Details:
The following antibodies were used: TLR4/CD284 (IMG-428E), TLR4/CD284 (IMG-577), and TLR4 (IMG-6307A). IMG-577 & IMG-6307A were used in Fig 1 (WB): Peritoneal primary mouse (BALB/C) macrophages stimulated with LPS and then treated with KML-C (Korean mistl
Tsukamoto H, K Fukudome, S Takao, N Tsuneyoshi, M Kimoto. Lipopolysaccharide-binding protein-mediated Toll-like receptor 4 dimerization enables rapid signal transduction against lipopolysaccharide stimulation on membrane-associated CD-14 expressing cells. Int Immuno 22: 271-280. 2010 [PMID: 20133493] (Flow-CS)

Details:
Products cited: 1. TLR4 (IMG-578): WB, TLR4 stably transfected Ba/F3 cells. Note: These cells were immunoprecipitated with a TLR4 mAb (clone UT41) then western blotted with the IMG-578A TLR4 pAb Fig 3B. 3. TLR4 (IMG-428A): Flow (cell surface), RAW264 and
Flow-CS
Macedo GC, Magnani DM, Carvalho NB et al. Central role of MyD88-dependent dendritic cell maturation and proinflammatory cytokine production to control Brucella abortus infection. J Immunol. 2008 Jan 15 [PMID: 18178848] (Flow, Mouse)

Details:
IMG-428E: FA (mouse bone marrow derived and splenic dentritic cells), Fig. 7C,D.
Flow Mouse
Corbucci C, Cenci E, Skrzypek F et al. Immune response to Candida albicans is preserved despite defect in O-mannosylation of secretory proteins. Med Mycol. 2007 Dec [PMID: 17885949] (Flow-CS)

Details:
Products cited (mouse macrophages): 1. IMG-428C (TLR4): Flow (Cell Surface), Fig. 5A 2. IMG-428E (TLR4): FA, Fig. 5B.
Flow-CS
El Shikh ME, El Sayed RM, Wu Y et al. TLR4 on follicular dendritic cells: an activation pathway that promotes accessory activity. J Immunol. 2007 Oct 1 [PMID: 17878340]

Details:
Products cited: 1. TLR4-FITC (IMG-428C): Flow (Cell Surface), Fig 1C, D [primary follicular dendritic (FDC) and B cells isolated from BALB/c mouse splenic leukocytes]. FDC cells expressed TLR4, but the B cells did not. 2. TLR4 (IMG-579A): IHC (F), Fig 1A
Konno K, Wakabayashi Y, Akashi-Takamura S et al. A molecule that is associated with Toll-like receptor 4 and regulates its cell surface expression. Biochem Biophys Res Commun. 2006 Jan 27 [PMID: 16338228] (IP)

Details:
1. IMG-428A (TLR4, Clone MTS510): IP (Figs 3 & 4), TLR4-transfected Ba/F3 cells. The antibody immunoprecipitated both the immature (less glycosylated) and mature (more glycosylated) forms of TLR4. 2. IMG-417A (TLR4, Clone HTA125): Flow (Cell Surface) (Fig
IP
Show All 13 Publications.

Review for TLR4 Antibody (NB100-56560) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-56560:
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 TLR4 NB100-56560
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reviewed by:
Jim Hsiao
Mouse 06/07/2017
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Summary

Sample TestedPrimary Mouse Lung Endothelial Cells
SpeciesMouse
LotAB032309A-01

Comments

CommentsTLR4 (MTS510): 20 ug/ml

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Blogs on TLR4.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR4: Playing Roles in Apoptosis and Autoimmunity
TLR4 (Toll-like receptor 4) is a member of the Toll-like receptor (TLR) family that plays a key role in pathogen recognition and activation of innate immunity. Scientists have found that TLRs are highly conserved from Drosophila to humans, with a high...  Read full blog post.

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Jim Hsiao
06/07/2017
Application:
Species: Mouse

Bioinformatics

Gene Symbol TLR4
Entrez
Uniprot